CF833010

Overview
NameCF833010
Unique NameCF833010
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length849
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF833010 vs. ExPASy Swiss-Prot
Match: METE_SERP5 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Serratia proteamaculans (strain 568) GN=metE PE=3 SV=1)

HSP 1 Score: 213.386 bits (542), Expect = 1.310e-54
Identity = 110/181 (60.77%), Postives = 132/181 (72.93%), Query Frame = -1 
Query:  286 QIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQLA 828
            QIALA++DEVEDLEKAGI +IQIDE ALREGLPLR+S+ A YL+WAV +F++     +D TQIHTHMCY  FNDI+ SI  +DADVITIE SRSD  LL  F+E  +Y   IGPGVYDIHSP +PS E I   + K    +    LWVNPDCGLKTR + E + +L+NMV AA+ LR Q A
Sbjct:  579 QIALALRDEVEDLEKAGIGIIQIDEPALREGLPLRQSDWAAYLNWAVDAFKLNAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMDLLEAFKE-FEYPNEIGPGVYDIHSPNVPSVEWIEALLRKAAQNIPAERLWVNPDCGLKTRGWPETRQSLANMVLAAQRLREQQA 758
[Close]
BLAST of CF833010 vs. ExPASy Swiss-Prot
Match: METE_PSEE4 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Pseudomonas entomophila (strain L48) GN=metE PE=3 SV=1)

HSP 1 Score: 213.386 bits (542), Expect = 1.310e-54
Identity = 109/177 (61.58%), Postives = 128/177 (72.32%), Query Frame = -1 
Query:  298 QIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLR 828
            Q+ALAI+DEV DLE AGI ++QIDEAA REGLPLR+S    YL+WA  +FR+    V+D TQIHTHMCYS FND+I SI  MDADVITIE SRSD +LL  F E   Y   IGPGVYDIHSPR+P+ EE+   + K    +    LWVNPDCGLKTR + E + AL NMVAAA+ LR
Sbjct:  584 QLALAIRDEVVDLEAAGIKIVQIDEAAFREGLPLRRSAWPHYLEWATEAFRLCASDVRDETQIHTHMCYSEFNDVIESIAAMDADVITIETSRSDMELLEAF-ERFDYPNEIGPGVYDIHSPRVPTKEEMVKLLRKAAQRIPAERLWVNPDCGLKTRAWPETEAALVNMVAAARELR 759
[Close]
BLAST of CF833010 vs. ExPASy Swiss-Prot
Match: METE_ACTPJ (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Actinobacillus pleuropneumoniae serotype 3 (strain JL03) GN=metE PE=3 SV=1)

HSP 1 Score: 213.386 bits (542), Expect = 1.310e-54
Identity = 109/188 (57.98%), Postives = 132/188 (70.21%), Query Frame = -1 
Query:  286 PRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQLA 849
            PR   C QI +A+ DEV DLEKAGI VIQIDE A+REGLPL++++   YL WA  +FR++  GV+D TQIHTHMCYS FNDI+ +I  +DADVITIE SRSD +LL+ F +  KY   IGPGVYDIHSPR+P+  EI   + K L V+    LWVNPDCGLKTR + E    L  MV   K LR +LA
Sbjct:  570 PRSTVCKQIGVALSDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLQWAGEAFRLSYMGVKDDTQIHTHMCYSEFNDILPAIAGLDADVITIETSRSDMELLTAFAD-FKYPNDIGPGVYDIHSPRVPTAGEIEHLLRKALKVIPKERLWVNPDCGLKTRGWKETIEQLQVMVEVTKKLRAELA 756
[Close]
BLAST of CF833010 vs. ExPASy Swiss-Prot
Match: METE_ACTP7 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Actinobacillus pleuropneumoniae serotype 7 (strain AP76) GN=metE PE=3 SV=1)

HSP 1 Score: 213.386 bits (542), Expect = 1.310e-54
Identity = 109/188 (57.98%), Postives = 132/188 (70.21%), Query Frame = -1 
Query:  286 PRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQLA 849
            PR   C QI +A+ DEV DLEKAGI VIQIDE A+REGLPL++++   YL WA  +FR++  GV+D TQIHTHMCYS FNDI+ +I  +DADVITIE SRSD +LL+ F +  KY   IGPGVYDIHSPR+P+  EI   + K L V+    LWVNPDCGLKTR + E    L  MV   K LR +LA
Sbjct:  570 PRSTVCKQIGVALSDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLQWAGEAFRLSYMGVKDDTQIHTHMCYSEFNDILPAIAGLDADVITIETSRSDMELLTAFAD-FKYPNDIGPGVYDIHSPRVPTAGEIEHLLRKALKVIPKERLWVNPDCGLKTRGWKETIEQLQVMVEVTKKLRAELA 756
[Close]
BLAST of CF833010 vs. ExPASy Swiss-Prot
Match: METE_ACTP2 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Actinobacillus pleuropneumoniae serotype 5b (strain L20) GN=metE PE=3 SV=1)

HSP 1 Score: 213.386 bits (542), Expect = 1.310e-54
Identity = 109/188 (57.98%), Postives = 132/188 (70.21%), Query Frame = -1 
Query:  286 PRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQLA 849
            PR   C QI +A+ DEV DLEKAGI VIQIDE A+REGLPL++++   YL WA  +FR++  GV+D TQIHTHMCYS FNDI+ +I  +DADVITIE SRSD +LL+ F +  KY   IGPGVYDIHSPR+P+  EI   + K L V+    LWVNPDCGLKTR + E    L  MV   K LR +LA
Sbjct:  570 PRSTVCKQIGVALSDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLQWAGEAFRLSYMGVKDDTQIHTHMCYSEFNDILPAIAGLDADVITIETSRSDMELLTAFAD-FKYPNDIGPGVYDIHSPRVPTAGEIEHLLRKALKVIPKERLWVNPDCGLKTRGWKETIEQLQVMVEVTKKLRAELA 756
[Close]
BLAST of CF833010 vs. ExPASy Swiss-Prot
Match: METE_VIBHB (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Vibrio harveyi (strain ATCC BAA-1116 / BB120) GN=metE PE=3 SV=1)

HSP 1 Score: 213.001 bits (541), Expect = 1.710e-54
Identity = 107/187 (57.22%), Postives = 134/187 (71.66%), Query Frame = -1 
Query:  286 RHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQLA 846
            R E   Q+ALA++DEV DL+ +GIN+IQIDE A+REGLPL+K +H  YL WAV +F+I+    +  TQIHTHMCYS FN+II S+  +DADVITIE SRS+ +LL  F E   Y   IGPGVYDIHSP IP+ E I   + K    +    LWVNPDCGLKTR + E + AL+N+V+AAK LRT+LA
Sbjct:  575 RKEIAEQLALALRDEVSDLQDSGINIIQIDEPAIREGLPLKKRDHDAYLQWAVDAFKISAASAKPETQIHTHMCYSEFNEIIDSVAALDADVITIETSRSNMELLKAF-EAFNYPNEIGPGVYDIHSPNIPTQEWIEALLKKAAEKVPVQRLWVNPDCGLKTRNWDETEAALANLVSAAKKLRTELA 760
[Close]
BLAST of CF833010 vs. ExPASy Swiss-Prot
Match: METE_PASMU (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Pasteurella multocida GN=metE PE=3 SV=1)

HSP 1 Score: 213.001 bits (541), Expect = 1.710e-54
Identity = 109/187 (58.29%), Postives = 131/187 (70.05%), Query Frame = -1 
Query:  289 PRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQL 849
            PR   C QI +A+ DEV DLEKAGI VIQIDE A+REGLPL++++   YL WA  +FR++  GVQD TQIHTHMCYS FNDI+ +I  +DADVITIE SRSD +LL+ F +  KY   IGPGVYDIHSPR+P  EEI   + K L V+    LWVNPDCGLKTR + E    L  M+   K LR +L
Sbjct:  571 PRSTVCKQIGVALSDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLQWAGEAFRLSYMGVQDDTQIHTHMCYSEFNDILPAIAGLDADVITIETSRSDMELLTAFGD-FKYPNDIGPGVYDIHSPRVPKAEEIERLLRKALNVVPKERLWVNPDCGLKTRGWPETIAQLEVMMEVTKKLRAEL 756
[Close]
BLAST of CF833010 vs. ExPASy Swiss-Prot
Match: METE_BIFAA (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083) GN=metE PE=3 SV=1)

HSP 1 Score: 213.001 bits (541), Expect = 1.710e-54
Identity = 110/182 (60.44%), Postives = 134/182 (73.63%), Query Frame = -1 
Query:  286 QIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSE-HAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQLA 828
            Q+ALAI+DEV DLEKAGI VIQIDEAALRE LPLRK++ H  YLDWA+ +FR+ +  V+ TTQIHTHMCYS FNDII  I  MDADVI+ E SR D  +L    +   +    GPGVYDIHSPRIPS +EI DRI ++L  ++   +W+NPDCGLKTR   E  P+L N+VAAAK +R +LA
Sbjct:  587 QLALAIRDEVLDLEKAGIKVIQIDEAALREKLPLRKTDWHKKYLDWAIPAFRLVHSAVKPTTQIHTHMCYSEFNDIIKDIDAMDADVISFEASRGDLVVLDAIHDA-NFETEAGPGVYDIHSPRIPSEQEIEDRIYEILKKMDVEKVWINPDCGLKTRGNAETWPSLENLVAAAKAVRAKLA 767
[Close]
BLAST of CF833010 vs. ExPASy Swiss-Prot
Match: METE_ACIF5 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Acidithiobacillus ferrooxidans (strain ATCC 53993) GN=metE PE=3 SV=1)

HSP 1 Score: 212.231 bits (539), Expect = 2.918e-54
Identity = 108/186 (58.06%), Postives = 129/186 (69.35%), Query Frame = -1 
Query:  292 PRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQ 849
            PR  T  QIALAI+DEV DL  AGI +IQIDE A REGLPL++ +   YL+WA  +FRI+     D  QIHTHMCYS FNDI+ +I  MDADVITIE SRS  +LL  F     Y   IGPGVYDIHSPR+PS EE+   + K + V+    LW+NPDCGLKTRK+ EV PAL NMV AA+ +R +
Sbjct:  598 PRERTALQIALAIRDEVRDLIDAGIGIIQIDEPAYREGLPLKRKDWGHYLEWASRAFRISAQIAPDDVQIHTHMCYSEFNDILPAIAAMDADVITIETSRSQMELLDAFAT-FNYPNEIGPGVYDIHSPRVPSVEEMVGLMEKAVKVVPAERLWINPDCGLKTRKWAEVTPALENMVEAARQVRAR 782
[Close]
BLAST of CF833010 vs. ExPASy Swiss-Prot
Match: METE_ACIF2 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) GN=metE PE=3 SV=1)

HSP 1 Score: 212.231 bits (539), Expect = 2.918e-54
Identity = 108/186 (58.06%), Postives = 129/186 (69.35%), Query Frame = -1 
Query:  292 PRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQ 849
            PR  T  QIALAI+DEV DL  AGI +IQIDE A REGLPL++ +   YL+WA  +FRI+     D  QIHTHMCYS FNDI+ +I  MDADVITIE SRS  +LL  F     Y   IGPGVYDIHSPR+PS EE+   + K + V+    LW+NPDCGLKTRK+ EV PAL NMV AA+ +R +
Sbjct:  598 PRERTALQIALAIRDEVRDLIDAGIGIIQIDEPAYREGLPLKRKDWGHYLEWASRAFRISAQIAPDDVQIHTHMCYSEFNDILPAIAAMDADVITIETSRSQMELLDAFAT-FNYPNEIGPGVYDIHSPRVPSVEEMVGLMEKAVKVVPAERLWINPDCGLKTRKWAEVTPALENMVEAARQVRAR 782
The following BLAST results are available for this feature:
BLAST of CF833010 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 283
Match NameE-valueIdentityDescription
METE_SERP51.310e-5460.775-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_PSEE41.310e-5461.585-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_ACTPJ1.310e-5457.985-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_ACTP71.310e-5457.985-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_ACTP21.310e-5457.985-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_VIBHB1.710e-5457.225-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_PASMU1.710e-5458.295-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BIFAA1.710e-5460.445-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_ACIF52.918e-5458.065-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_ACIF22.918e-5458.065-methyltetrahydropteroyltriglutamate--homocystein... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionUCRCS02_02N01_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa02N01, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF833010 ID=CF833010; Name=CF833010; organism=Citrus sinensis; type=EST; length=849bp
TTTTTTTTTTTTTTATGCACAAACCAGTAAAGGTATTCCATCGATTTACG
GCCACTGATAGCAAGCCGGGGCCTTTAAAAACGGAAAATACAAGCAAAGA
AAAATAGAAGCCAATTGCTCAATAGTGCATAATATGCATACGGAAAATTT
TCATCACATCACGACCCACAGTTATTCGAATAGGTAATAAGACAGGAATT
TTCTTTCTTTGTTTAAAGTGAAAAAAAAAAAAATCAATGGCTCAGGAAGT
ATCCCTCCAATCTCCAGAACCACTCACTTGGCACTGGCAAGCTGGGTGCG
GAGTAGCTTGGCAGCAGCGACCATGTTTGAGAGGGCTGGCTTCACCTCAG
TGTACTTGCGAGTCTTAAGACCACAGTCAGGGTTAACCCACAAAATGTTG
GTTTCTAGCACAGCAAGCATCTTGTTGATTCTGTCAGCAATTTCTTCAGT
TGATGGTATTCTTGGAGAGTGGATGTCATACACACCAGGGCCAATTCCAG
CACCATACTTGACTCCCTCACGGAAGACAGACAGGAGCTTCTCATCAGAA
CGAGAGTTCTCAATGGTGATCACATCAGCATCCATGTCGATAATTGAGTG
GATAATGTCGTTGAAGTTGGAGTAGCACATGTGGGTGTGGATCTGGGTAG
TGTCCTGGACGCCACAGTTGGTGATTCTGAAGGAGTGGACTGCCCAGTCC
AAGTAGAAAGCATGCTCAGATTTCCTAAGAGGCAATCCTTCCCTCAAGGC
AGCCTCATCAATTTGAATAACGTTAATGCCTGCCTTCTCAAGATCCTCGA
CTTCGTCCTTGATGGCCAAAGCAATCTGGTAGCAGGTTTCATGTCTGGG
back to top