CF833115

Overview
NameCF833115
Unique NameCF833115
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length800
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF833115 vs. ExPASy Swiss-Prot
Match: GLYA_RALEH (Serine hydroxymethyltransferase OS=Ralstonia eutropha (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) GN=glyA PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.343e-18
Identity = 48/115 (41.74%), Postives = 69/115 (60.00%), Query Frame = -3 
Query:  457 IGALAVALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVTGGTENHLVFWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGD-SSALAPGGVRIGTPAMTSRGLLEKDFEQIG 798
            I   AVA K+A TP FK Y +QV  NA  L   L  +G  +V+G TE+H++  DLR   +TG + E++    +ITVNKNA+  D        G+R+G+PAMT+RG  E++   +G
Sbjct:  267 IAGKAVAFKEALTPEFKEYQQQVVKNAAVLAETLIARGLRIVSGRTESHVMLVDLRAKNITGKEAERILGEAHITVNKNAIPNDPEKPFVTSGIRLGSPAMTTRGFKEEEARIVG 381
[Close]
BLAST of CF833115 vs. ExPASy Swiss-Prot
Match: GLYA_PSEA6 (Serine hydroxymethyltransferase OS=Pseudoalteromonas atlantica (strain T6c / BAA-1087) GN=glyA PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.343e-18
Identity = 51/114 (44.74%), Postives = 69/114 (60.53%), Query Frame = -3 
Query:  460 IGALAVALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVTGGTENHLVFWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGD-SSALAPGGVRIGTPAMTSRGLLEKDFEQI 798
            I A AVA K+A  P FKAY +QV  NA A+ + +  +GY +V+GGT+NHL   DL    +TG   +      NITVNKN+V  D  S     G+RIG+PA+T RG  E+  +Q+
Sbjct:  270 IAAKAVAFKEALQPDFKAYQQQVLLNAKAMVSVMQERGYDIVSGGTDNHLFLLDLISKDITGKDADAALGRANITVNKNSVPNDPRSPFVTSGLRIGSPAITRRGFKEEQAKQV 383
[Close]
BLAST of CF833115 vs. ExPASy Swiss-Prot
Match: GLYA_PROM9 (Serine hydroxymethyltransferase OS=Prochlorococcus marinus (strain MIT 9312) GN=glyA PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.343e-18
Identity = 50/116 (43.10%), Postives = 68/116 (58.62%), Query Frame = -3 
Query:  454 IGALAVALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVTGGTENHLVFWDLRPLGLTGNKVEKLCDLCNITVNKNAV-FGDSSALAPGGVRIGTPAMTSRGLLEKDFEQIGE 798
            I A AVA  +A  P F  Y++QV  NA  L + L  +G  +V+GGT+NH+V  DLR + +TG   + L    NIT NKN V F   S     G+R+GT A+T+RG  E  F ++GE
Sbjct:  267 IAAKAVAFGEALQPDFVNYSQQVIKNAKVLASTLISRGIDIVSGGTDNHIVLLDLRSINMTGKIADLLVSAVNITANKNTVPFDPESPFVTSGLRLGTAALTTRGFNETAFAEVGE 382
[Close]
BLAST of CF833115 vs. ExPASy Swiss-Prot
Match: GLYA_PROM5 (Serine hydroxymethyltransferase OS=Prochlorococcus marinus (strain MIT 9515) GN=glyA PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.343e-18
Identity = 50/116 (43.10%), Postives = 69/116 (59.48%), Query Frame = -3 
Query:  454 IGALAVALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVTGGTENHLVFWDLRPLGLTGNKVEKLCDLCNITVNKNAV-FGDSSALAPGGVRIGTPAMTSRGLLEKDFEQIGE 798
            I A AVA  +A  P F  Y+KQV  NA  L + L  +G  +V+GGT+NH+V  DLR + +TG   + L    NIT NKN V F   S     G+R+GT A+T+RG  ++ F ++GE
Sbjct:  267 IAAKAVAFGEALQPNFVNYSKQVIKNAKVLSSTLINRGIDIVSGGTDNHIVLLDLRSINMTGKVADLLVSEVNITANKNTVPFDPESPFVTSGLRLGTAALTTRGFNDEAFIEVGE 382
[Close]
BLAST of CF833115 vs. ExPASy Swiss-Prot
Match: GLYA_MYCCT (Serine hydroxymethyltransferase OS=Mycoplasma capricolum subsp. capricolum (strain California kid / ATCC 27343 / NCTC 10154) GN=glyA PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.343e-18
Identity = 49/110 (44.55%), Postives = 67/110 (60.91%), Query Frame = -3 
Query:  457 ALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVTGGTENHLVFWDLR-PLGLTGNKVEKLCDLCNITVNKNAV-FGDSSALAPGGVRIGTPAMTSRGLLEKDFEQIG 780
            AL +AST  FK Y KQ+  N  AL N L   G  LV GG++NHL+  D++  L +TG K EK+ +   I  NKN + F         G+R+GTPAMT+RG  E++F+Q+G
Sbjct:  267 ALLEASTDEFKEYGKQIVKNTKALANVLQENGLRLVAGGSDNHLINVDIKSTLQITGKKAEKILESIGIICNKNMIPFDTEKPFYTSGIRLGTPAMTTRGFKEEEFKQVG 376
[Close]
BLAST of CF833115 vs. ExPASy Swiss-Prot
Match: GLYA_LISMH (Serine hydroxymethyltransferase OS=Listeria monocytogenes serotype 4a (strain HCC23) GN=glyA PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.343e-18
Identity = 49/115 (42.61%), Postives = 68/115 (59.13%), Query Frame = -3 
Query:  457 IGALAVALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVTGGTENHLVFWDLRPLGLTGNKVEKLCDLCNITVNKNAV-FGDSSALAPGGVRIGTPAMTSRGLLEKDFEQIG 798
            I A AVA  +A  P F AY +Q+  N+  L   L     +++TGG++NHL+  DL+PLGLTG   EK+ D   ITVNKN + F   S     G+R+G  A+T+RG  E   E++G
Sbjct:  263 IAAKAVAFGEALQPEFTAYCEQIIRNSKKLAETLQANDVAVLTGGSDNHLLLIDLKPLGLTGKAAEKVLDEVGITVNKNTIPFETESPFVTSGIRVGVAAVTTRGFDEVAIEKVG 377
[Close]
BLAST of CF833115 vs. ExPASy Swiss-Prot
Match: GLYA_LISMF (Serine hydroxymethyltransferase OS=Listeria monocytogenes serotype 4b (strain F2365) GN=glyA PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.343e-18
Identity = 49/115 (42.61%), Postives = 68/115 (59.13%), Query Frame = -3 
Query:  457 IGALAVALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVTGGTENHLVFWDLRPLGLTGNKVEKLCDLCNITVNKNAV-FGDSSALAPGGVRIGTPAMTSRGLLEKDFEQIG 798
            I A AVA  +A  P F AY +Q+  N+  L   L     +++TGG++NHL+  DL+PLGLTG   EK+ D   ITVNKN + F   S     G+R+G  A+T+RG  E   E++G
Sbjct:  263 IAAKAVAFGEALQPEFTAYCEQIIRNSKKLAETLQANDVAVLTGGSDNHLLLIDLKPLGLTGKAAEKVLDEVGITVNKNTIPFETESPFVTSGIRVGVAAVTTRGFDEVAIEKVG 377
[Close]
BLAST of CF833115 vs. ExPASy Swiss-Prot
Match: GLYA_LISMC (Serine hydroxymethyltransferase OS=Listeria monocytogenes serotype 4b (strain Clip81459) GN=glyA PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.343e-18
Identity = 49/115 (42.61%), Postives = 68/115 (59.13%), Query Frame = -3 
Query:  457 IGALAVALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVTGGTENHLVFWDLRPLGLTGNKVEKLCDLCNITVNKNAV-FGDSSALAPGGVRIGTPAMTSRGLLEKDFEQIG 798
            I A AVA  +A  P F AY +Q+  N+  L   L     +++TGG++NHL+  DL+PLGLTG   EK+ D   ITVNKN + F   S     G+R+G  A+T+RG  E   E++G
Sbjct:  263 IAAKAVAFGEALQPEFTAYCEQIIRNSKKLAETLQANDVAVLTGGSDNHLLLIDLKPLGLTGKAAEKVLDEVGITVNKNTIPFETESPFVTSGIRVGVAAVTTRGFDEVAIEKVG 377
[Close]
BLAST of CF833115 vs. ExPASy Swiss-Prot
Match: GLYA_LISIN (Serine hydroxymethyltransferase OS=Listeria innocua GN=glyA PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.343e-18
Identity = 49/115 (42.61%), Postives = 68/115 (59.13%), Query Frame = -3 
Query:  457 IGALAVALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVTGGTENHLVFWDLRPLGLTGNKVEKLCDLCNITVNKNAV-FGDSSALAPGGVRIGTPAMTSRGLLEKDFEQIG 798
            I A AVA  +A  P F AY +Q+  N+  L   L     +++TGG++NHL+  DL+PLGLTG   EK+ D   ITVNKN + F   S     G+R+G  A+T+RG  E   E++G
Sbjct:  263 IAAKAVAFGEALQPEFTAYCEQIIRNSKKLAETLQANDVAVLTGGSDNHLLLIDLKPLGLTGKAAEKVLDEVGITVNKNTIPFETESPFVTSGIRVGVAAVTTRGFDEVAIEKVG 377
[Close]
BLAST of CF833115 vs. ExPASy Swiss-Prot
Match: GLYA_HYDS0 (Serine hydroxymethyltransferase OS=Hydrogenobaculum sp. (strain Y04AAS1) GN=glyA PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.343e-18
Identity = 51/122 (41.80%), Postives = 72/122 (59.02%), Query Frame = -3 
Query:  436 IGALAVALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVTGGTENHLVFWDLRPLGLTGNKVEKLCDLCNITVNKNAV-FGDSSALAPGGVRIGTPAMTSRGLLEKDFEQIGEFFHRAV 798
            I A AV  K+A +  FK YA+QV  NA  L   L  +G +++TGGT++H+V  DLR +G+TG + E       ITVNKNAV F         G+RIGTPA+T+RG+ E   + I +   + +
Sbjct:  262 IAAKAVCFKEAMSDEFKQYAQQVVENARVLAEELLKEGINVLTGGTDSHMVLVDLRNIGITGKEAENRLGEAGITVNKNAVPFDPLPPTKTSGIRIGTPALTTRGMKEDQMKIIAKIIAKVL 383
The following BLAST results are available for this feature:
BLAST of CF833115 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
GLYA_RALEH2.343e-1841.74Serine hydroxymethyltransferase OS=Ralstonia eutro... [more]
GLYA_PSEA62.343e-1844.74Serine hydroxymethyltransferase OS=Pseudoalteromon... [more]
GLYA_PROM92.343e-1843.10Serine hydroxymethyltransferase OS=Prochlorococcus... [more]
GLYA_PROM52.343e-1843.10Serine hydroxymethyltransferase OS=Prochlorococcus... [more]
GLYA_MYCCT2.343e-1844.55Serine hydroxymethyltransferase OS=Mycoplasma capr... [more]
GLYA_LISMH2.343e-1842.61Serine hydroxymethyltransferase OS=Listeria monocy... [more]
GLYA_LISMF2.343e-1842.61Serine hydroxymethyltransferase OS=Listeria monocy... [more]
GLYA_LISMC2.343e-1842.61Serine hydroxymethyltransferase OS=Listeria monocy... [more]
GLYA_LISIN2.343e-1842.61Serine hydroxymethyltransferase OS=Listeria innocu... [more]
GLYA_HYDS02.343e-1841.80Serine hydroxymethyltransferase OS=Hydrogenobaculu... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_03A20_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa03A20, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF833115 ID=CF833115; Name=CF833115; organism=Citrus sinensis; type=EST; length=800bp
TTTTTTTTTTTTTTTTTAAGAAATAAAATACATAAATTTGTTCTTTTATT
TGTAGATGAACAATCATCATTTGACCAAAGAGAAACGGATCTCACTTTCA
CAATATGGTGGGTACCAATTAAAAAAAAAAAAAAAGTTAAAACGAAAGCG
TTTAAACCTAGGACAACAAAAGGCAAAGATGCATGCAATTTTTTTTCTTC
TCTTTCTGTTTTTCAACACTAAAGAGTTGCTAACAACACAGTAACAATAA
TGATGATACTTTGAGACCTAATCCTTGTACTTCATCTCGGACATTTTAAA
GCCGGGCATGTCAAATGAGCTGGCGAACTTCTCAACATCAGCCTTGAGGG
CCTCGATGTCCTTGTTGTTCACCAAACCCTTGTTGAAGTCCTTCAAGAGC
TTTCCATACTCCTTTTGAATTTCCAACGTGAGGGTCACAGCGCGGTGAAA
AAACTCTCCAATTTGCTCGAAGTCCTTCTCCAACAAACCTCTGGAAGTCA
TGGCAGGAGTACCGATTCTTACTCCTCCAGGGGCCAAGGCACTGCTGTCA
CCAAACACAGCATTCTTGTTCACAGTAATGTTGCAAAGGTCACAGAGCTT
CTCAACCTTATTGCCAGTCAATCCAAGAGGGCGTAAATCCCAAAAAACAA
GGTGGTTCTCAGTTCCACCAGTGACAAGGCTGTATCCCTTGCCCGTCAGG
TAGTTCCCAAGTGCAACTGCATTGGCCTTGACTTGTTTCGCATAGGCCTT
GAATGCAGGGGTCGAGGCCTGTTTCAGGGCAACAGCTAGAGCACCAATCT
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