CF833314

Overview
NameCF833314
Unique NameCF833314
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length741
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF833314 vs. ExPASy Swiss-Prot
Match: UB2D1_MOUSE (Ubiquitin-conjugating enzyme E2 D1 OS=Mus musculus GN=Ube2d1 PE=2 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 8.665e-33
Identity = 69/143 (48.25%), Postives = 94/143 (65.73%), Query Frame = -1 
Query:  268 RRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYA 696
            +RI KE   L  +P    SA P  D++ ++   I+GP  S Y+GGVF L +  P +YP   PK+ F TKIYHPNI+  G ICLDIL+ +WSPAL +  VLLSI +LL  PNPDDPL  +IA+ +K+++ +    A+EWT+ YA
Sbjct:    4 KRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYPFKPPKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPDIAQIYKSDKEKYNRHAREWTQKYA 146
[Close]
BLAST of CF833314 vs. ExPASy Swiss-Prot
Match: UB2D1_HUMAN (Ubiquitin-conjugating enzyme E2 D1 OS=Homo sapiens GN=UBE2D1 PE=1 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 8.665e-33
Identity = 69/143 (48.25%), Postives = 94/143 (65.73%), Query Frame = -1 
Query:  268 RRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYA 696
            +RI KE   L  +P    SA P  D++ ++   I+GP  S Y+GGVF L +  P +YP   PK+ F TKIYHPNI+  G ICLDIL+ +WSPAL +  VLLSI +LL  PNPDDPL  +IA+ +K+++ +    A+EWT+ YA
Sbjct:    4 KRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYPFKPPKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPDIAQIYKSDKEKYNRHAREWTQKYA 146
[Close]
BLAST of CF833314 vs. ExPASy Swiss-Prot
Match: UB2D1_BOVIN (Ubiquitin-conjugating enzyme E2 D1 OS=Bos taurus GN=UBE2D1 PE=2 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 8.665e-33
Identity = 69/143 (48.25%), Postives = 94/143 (65.73%), Query Frame = -1 
Query:  268 RRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYA 696
            +RI KE   L  +P    SA P  D++ ++   I+GP  S Y+GGVF L +  P +YP   PK+ F TKIYHPNI+  G ICLDIL+ +WSPAL +  VLLSI +LL  PNPDDPL  +IA+ +K+++ +    A+EWT+ YA
Sbjct:    4 KRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYPFKPPKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPDIAQIYKSDKEKYNRHAREWTQKYA 146
[Close]
BLAST of CF833314 vs. ExPASy Swiss-Prot
Match: UB2E3_MOUSE (Ubiquitin-conjugating enzyme E2 E3 OS=Mus musculus GN=Ube2e3 PE=1 SV=2)

HSP 1 Score: 139.813 bits (351), Expect = 1.478e-32
Identity = 74/148 (50.00%), Postives = 92/148 (62.16%), Query Frame = -1 
Query:  265 SNLPRRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYAS 708
            S   +RI KE   +  +P P  SA P  DN+  +   ILGP  S YEGGVF L++    +YP   PKV F T+IYH NI+  G ICLDILKD WSPAL I  VLLSI +LL+  NP DPL  +IA  + TN AE    A++WT+ YA+
Sbjct:   60 STSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSSDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYLTNRAEHDRIARQWTKRYAT 207
[Close]
BLAST of CF833314 vs. ExPASy Swiss-Prot
Match: UB2E3_HUMAN (Ubiquitin-conjugating enzyme E2 E3 OS=Homo sapiens GN=UBE2E3 PE=1 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 1.478e-32
Identity = 74/148 (50.00%), Postives = 92/148 (62.16%), Query Frame = -1 
Query:  265 SNLPRRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYAS 708
            S   +RI KE   +  +P P  SA P  DN+  +   ILGP  S YEGGVF L++    +YP   PKV F T+IYH NI+  G ICLDILKD WSPAL I  VLLSI +LL+  NP DPL  +IA  + TN AE    A++WT+ YA+
Sbjct:   60 STSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSSDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYLTNRAEHDRIARQWTKRYAT 207
[Close]
BLAST of CF833314 vs. ExPASy Swiss-Prot
Match: UB2E3_BOVIN (Ubiquitin-conjugating enzyme E2 E3 OS=Bos taurus GN=UBE2E3 PE=2 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 1.478e-32
Identity = 74/148 (50.00%), Postives = 92/148 (62.16%), Query Frame = -1 
Query:  265 SNLPRRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYAS 708
            S   +RI KE   +  +P P  SA P  DN+  +   ILGP  S YEGGVF L++    +YP   PKV F T+IYH NI+  G ICLDILKD WSPAL I  VLLSI +LL+  NP DPL  +IA  + TN AE    A++WT+ YA+
Sbjct:   60 STSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSSDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYLTNRAEHDRIARQWTKRYAT 207
[Close]
BLAST of CF833314 vs. ExPASy Swiss-Prot
Match: UB2E2_MOUSE (Ubiquitin-conjugating enzyme E2 E2 OS=Mus musculus GN=Ube2e2 PE=2 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 1.478e-32
Identity = 74/148 (50.00%), Postives = 92/148 (62.16%), Query Frame = -1 
Query:  265 SNLPRRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYAS 708
            S   +RI KE   +  +P P  SA P  DN+  +   ILGP  S YEGGVF L++    +YP   PKV F T+IYH NI+  G ICLDILKD WSPAL I  VLLSI +LL+  NP DPL  +IA  + TN AE    A++WT+ YA+
Sbjct:   54 STSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYAT 201
[Close]
BLAST of CF833314 vs. ExPASy Swiss-Prot
Match: UB2E2_HUMAN (Ubiquitin-conjugating enzyme E2 E2 OS=Homo sapiens GN=UBE2E2 PE=1 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 1.478e-32
Identity = 74/148 (50.00%), Postives = 92/148 (62.16%), Query Frame = -1 
Query:  265 SNLPRRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYAS 708
            S   +RI KE   +  +P P  SA P  DN+  +   ILGP  S YEGGVF L++    +YP   PKV F T+IYH NI+  G ICLDILKD WSPAL I  VLLSI +LL+  NP DPL  +IA  + TN AE    A++WT+ YA+
Sbjct:   54 STSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYAT 201
[Close]
BLAST of CF833314 vs. ExPASy Swiss-Prot
Match: UBC12_ARATH (Probable ubiquitin-conjugating enzyme E2 12 OS=Arabidopsis thaliana GN=UBC12 PE=2 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 1.251e-31
Identity = 67/146 (45.89%), Postives = 95/146 (65.07%), Query Frame = -1 
Query:  262 RRIIKETQRLLSEPAPGISASP-SEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYASG 696
            +RI +E + +   P    SA P +E+++ ++   I+GP  SPY GGVF + +    +YP   PKV F TK+YHPNID  G ICLDILK++WSPA     VLLSI +LL+ PNP+DPL   IA  +K ++++   TA++WT+ YA G
Sbjct:    4 KRISRELRDMQRHPPANCSAGPVAEEDIFHWQATIMGPHDSPYSGGVFTVSIDFSSDYPFKPPKVNFKTKVYHPNIDSKGSICLDILKEQWSPAPTTSKVLLSICSLLTDPNPNDPLVPEIAHLYKVDKSKYESTAQKWTQKYAMG 149
[Close]
BLAST of CF833314 vs. ExPASy Swiss-Prot
Match: UBC1_YEAST (Ubiquitin-conjugating enzyme E2-24 kDa OS=Saccharomyces cerevisiae GN=UBC1 PE=1 SV=1)

HSP 1 Score: 134.035 bits (336), Expect = 8.110e-31
Identity = 65/146 (44.52%), Postives = 96/146 (65.75%), Query Frame = -1 
Query:  265 RRIIKETQRLLSEPAPGISAS-PSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL-GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYAS 696
            +RI+KE Q +  +PA  I+    SE ++ +     LGP  +PYEGG F +++ +P EYP   PK++F TK+YHPNI  + G ICLDILK+ WSP + +++ L+S+QALL +P P+DP    +A+H+  +     +TA  WTRLYAS
Sbjct:    5 KRIMKEIQAVKDDPAAHITLEFVSESDIHHLKGTFLGPPGTPYEGGKFVVDIEVPMEYPFKPPKMQFDTKVYHPNISSVTGAICLDILKNAWSPVITLKSALISLQALLQSPEPNDPQDAEVAQHYLRDRESFNKTAALWTRLYAS 150
The following BLAST results are available for this feature:
BLAST of CF833314 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 250
Match NameE-valueIdentityDescription
UB2D1_MOUSE8.665e-3348.25Ubiquitin-conjugating enzyme E2 D1 OS=Mus musculus... [more]
UB2D1_HUMAN8.665e-3348.25Ubiquitin-conjugating enzyme E2 D1 OS=Homo sapiens... [more]
UB2D1_BOVIN8.665e-3348.25Ubiquitin-conjugating enzyme E2 D1 OS=Bos taurus G... [more]
UB2E3_MOUSE1.478e-3250.00Ubiquitin-conjugating enzyme E2 E3 OS=Mus musculus... [more]
UB2E3_HUMAN1.478e-3250.00Ubiquitin-conjugating enzyme E2 E3 OS=Homo sapiens... [more]
UB2E3_BOVIN1.478e-3250.00Ubiquitin-conjugating enzyme E2 E3 OS=Bos taurus G... [more]
UB2E2_MOUSE1.478e-3250.00Ubiquitin-conjugating enzyme E2 E2 OS=Mus musculus... [more]
UB2E2_HUMAN1.478e-3250.00Ubiquitin-conjugating enzyme E2 E2 OS=Homo sapiens... [more]
UBC12_ARATH1.251e-3145.89Probable ubiquitin-conjugating enzyme E2 12 OS=Ara... [more]
UBC1_YEAST8.110e-3144.52Ubiquitin-conjugating enzyme E2-24 kDa OS=Saccharo... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_03G16_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa03G16, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF833314 ID=CF833314; Name=CF833314; organism=Citrus sinensis; type=EST; length=741bp
CCCTTTTTTTTTTTTTTGCTTGAAATTTAAGAGTTGAGAAACAATGTATA
TCATCTCCTTTTACAAGTTCAAAGACATGGCTTCCACAAGAAGAAAATAC
TTGACATGTGCGGTAAGATGGAAGCTTTCAGAATGTCAAACTCCGAATGT
TCAAACATCAATTCAATTCTTCCAAATTTTCCATTTTAAACAGACAGTGA
ATAGCATACATTGCACAATTGTTATTTGAATGAAGTGATATTTTCCACCA
TGTCATCAAGCACCACTGGCATATAAACGTGTCCATTCCTTCGCTGTTTC
AACTGCTTCGGCCTCATTTGTTTTCCAGTGTTTGGCAATGTTCTCGGAGA
GAGGATCATCGGGGTTAGGAGCACTCAGAAGTGCTTGAATGCTCAACAGT
ACAGTCCGAATCTGGAGAGCTGGACTCCACTTGTCTTTCAGAATATCAAG
ACAAATCCTTCCAAGCTTATCAATGTTAGGATGATATATCTTGGTAAGAA
ATCGGACCTTTGGAGCAGCCATTGGATATTCTTCAGGCAAAAATAATTCC
AATTTGAACACCCCTCCTTCATATGGAGATTGCGTCGGACCAAGGATCAT
CACATTAAAGTATCGCATATTATCTTCCGAGGGAGATGCACTTATTCCTG
GTGCTGGTTCACTGAGGAGACGCTGTGTTTCCTTGATGATACGTCGAGGG
AGATTACTGTTCGCCATTTCCAAGATCGATTTTTTTTGTTT
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