CF834625

Overview
NameCF834625
Unique NameCF834625
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length654
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CF834625_ssr54 microsatellite CF834625_ssr54:54..69. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig10947 contig Csv1_Contig10947:155..805. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF834625 vs. ExPASy Swiss-Prot
Match: METK2_HORVU (S-adenosylmethionine synthetase 2 OS=Hordeum vulgare GN=SAM2 PE=2 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 2.795e-74
Identity = 137/164 (83.54%), Postives = 146/164 (89.02%), Query Frame = -1 
Query:  163 FHLNPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 654
            FHLNPSGRFVIGGPHGDAGLTGRKIIIDTY            GKDPTKVDRSGAYI RQAAKSI+ASGLARRCIVQ+SYAIGVPEPLSVFVDSYGTGKIPD+EILK+VKENFDFRPGMISINLDLK+GG  RF+KTAAYGHFGRDD DFTWE+VKPLK++K  A
Sbjct:  232 FHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIARQAAKSIIASGLARRCIVQISYAIGVPEPLSVFVDSYGTGKIPDREILKLVKENFDFRPGMISINLDLKKGGK-RFIKTAAYGHFGRDDADFTWEVVKPLKFDKASA 394
[Close]
BLAST of CF834625 vs. ExPASy Swiss-Prot
Match: METK3_ATRNU (S-adenosylmethionine synthetase 3 OS=Atriplex nummularia GN=SAMS3 PE=1 SV=1)

HSP 1 Score: 276.944 bits (707), Expect = 6.226e-74
Identity = 138/164 (84.15%), Postives = 146/164 (89.02%), Query Frame = -1 
Query:  163 FHLNPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 654
            FHLNPSGRFVIGGPHGDAGLTGRKIIIDTY            GKDPTKVDRSGAYI RQAAKSIVA+GLARRCIVQ+SYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKE+FDFRPGMI+INLDL +GG+ R+LKTAAYGHFGRDD DFTWE VKPLKWEK QA
Sbjct:  234 FHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIARQAAKSIVAAGLARRCIVQISYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKESFDFRPGMIAINLDLLKGGS-RYLKTAAYGHFGRDDADFTWETVKPLKWEKPQA 396
[Close]
BLAST of CF834625 vs. ExPASy Swiss-Prot
Match: METK1_PEA (S-adenosylmethionine synthetase 1 (Fragment) OS=Pisum sativum GN=SAMS1 PE=2 SV=2)

HSP 1 Score: 276.944 bits (707), Expect = 6.226e-74
Identity = 137/160 (85.62%), Postives = 142/160 (88.75%), Query Frame = -1 
Query:  172 HLNPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEK 651
            HLNPSGRFVIGGPHGDAGLTGRKIIIDTY            GKDPTKVDR GAYIVRQAAKSIVASGLARR IVQ+  AIGVPEPLSVFVD+YGTGKIPD+EILKIVKE FDFRPGMISINLDL RGGNGRFLKTAAYGHFGR+DPDFTWE+VKPLKWEK
Sbjct:  206 HLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRRGAYIVRQAAKSIVASGLARRAIVQLLRAIGVPEPLSVFVDTYGTGKIPDREILKIVKETFDFRPGMISINLDLLRGGNGRFLKTAAYGHFGREDPDFTWEVVKPLKWEK 365
[Close]
BLAST of CF834625 vs. ExPASy Swiss-Prot
Match: METK1_HORVU (S-adenosylmethionine synthetase 1 OS=Hordeum vulgare GN=SAM1 PE=2 SV=1)

HSP 1 Score: 276.944 bits (707), Expect = 6.226e-74
Identity = 136/164 (82.93%), Postives = 146/164 (89.02%), Query Frame = -1 
Query:  163 FHLNPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 654
            FHLNPSGRFVIGGPHGDAGLT RKIIIDTY            GKDPTKVDRSGAYI RQAAKSI+ASGLARRCIVQ+SYAIGVPEPLSVFVDSYGTGKIPD+EILK+VKENFDFRPGMI+INLDLK+GGN RF+KTAAYGHFGRDD DFTWE+VKPLK++K  A
Sbjct:  232 FHLNPSGRFVIGGPHGDAGLTARKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIARQAAKSIIASGLARRCIVQISYAIGVPEPLSVFVDSYGTGKIPDREILKLVKENFDFRPGMITINLDLKKGGN-RFIKTAAYGHFGRDDADFTWEVVKPLKFDKASA 394
[Close]
BLAST of CF834625 vs. ExPASy Swiss-Prot
Match: METK4_ATRNU (S-adenosylmethionine synthetase 4 OS=Atriplex nummularia GN=SAMS4 PE=1 SV=1)

HSP 1 Score: 276.559 bits (706), Expect = 8.131e-74
Identity = 138/164 (84.15%), Postives = 145/164 (88.41%), Query Frame = -1 
Query:  163 FHLNPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 654
            FHLNPSGRFVIGGPHGDAGLTGRKIIIDTY            GKDPTKVDRSGAYI RQAAKSIVA+GLARRCIVQ+SYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKE FDFRPGMI+INLDL +GG+ R+LKTAAYGHFGRDD DFTWE VKPLKWEK QA
Sbjct:  234 FHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIARQAAKSIVAAGLARRCIVQISYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKETFDFRPGMIAINLDLLKGGS-RYLKTAAYGHFGRDDADFTWETVKPLKWEKPQA 396
[Close]
BLAST of CF834625 vs. ExPASy Swiss-Prot
Match: METK2_ATRNU (S-adenosylmethionine synthetase 2 OS=Atriplex nummularia GN=SAMS2 PE=1 SV=1)

HSP 1 Score: 276.559 bits (706), Expect = 8.131e-74
Identity = 138/164 (84.15%), Postives = 145/164 (88.41%), Query Frame = -1 
Query:  163 FHLNPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 654
            FHLNPSGRFVIGGPHGDAGLTGRKIIIDTY            GKDPTKVDRSGAYI RQAAKSIVA+GLARRCIVQ+SYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKE FDFRPGMI+INLDL +GG+ R+LKTAAYGHFGRDD DFTWE VKPLKWEK QA
Sbjct:  234 FHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIARQAAKSIVAAGLARRCIVQISYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKETFDFRPGMIAINLDLLKGGS-RYLKTAAYGHFGRDDADFTWETVKPLKWEKPQA 396
[Close]
BLAST of CF834625 vs. ExPASy Swiss-Prot
Match: METK1_DAUCA (S-adenosylmethionine synthetase 1 OS=Daucus carota GN=SAMS1 PE=2 SV=1)

HSP 1 Score: 276.559 bits (706), Expect = 8.131e-74
Identity = 148/212 (69.81%), Postives = 150/212 (70.75%), Query Frame = -1 
Query:  163 FHLNPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSY------------------------------------------------GTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 654
            FHLNPSGRFVIGGPHGDAGLTGRKIIIDTY            GKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSY                                                GTGKIPD+EILKIVKE FDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE+VKPLKWEK QA
Sbjct:  230 FHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDREILQIVKETFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGTGKIPDREILKIVKETFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 441
[Close]
BLAST of CF834625 vs. ExPASy Swiss-Prot
Match: METK1_ATRNU (S-adenosylmethionine synthetase 1 OS=Atriplex nummularia GN=SAMS1 PE=1 SV=2)

HSP 1 Score: 276.559 bits (706), Expect = 8.131e-74
Identity = 138/164 (84.15%), Postives = 145/164 (88.41%), Query Frame = -1 
Query:  163 FHLNPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 654
            FHLNPSGRFVIGGPHGDAGLTGRKIIIDTY            GKDPTKVDRSGAYI RQAAKSIVA+GLARRCIVQ+SYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKE FDFRPGMI+INLDL +GG+ R+LKTAAYGHFGRDD DFTWE VKPLKWEK QA
Sbjct:  234 FHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIARQAAKSIVAAGLARRCIVQISYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKETFDFRPGMIAINLDLLKGGS-RYLKTAAYGHFGRDDADFTWETVKPLKWEKPQA 396
[Close]
BLAST of CF834625 vs. ExPASy Swiss-Prot
Match: METK_CAMSI (S-adenosylmethionine synthetase OS=Camellia sinensis GN=SAM PE=2 SV=1)

HSP 1 Score: 273.863 bits (699), Expect = 5.271e-73
Identity = 135/152 (88.82%), Postives = 139/152 (91.45%), Query Frame = -1 
Query:  199 FHLNPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 654
            FHLNPSGRFVIGGPHGDAGLTGRKIIIDTY            GKDPTKVDRSGAYIVRQAAKSIVA+GLARRCIVQVSYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKE+FDFRPGMI+INLDLKRGGN RFLKTAAYGHFGRDDPDFTWE
Sbjct:  230 FHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKESFDFRPGMIAINLDLKRGGNSRFLKTAAYGHFGRDDPDFTWE 381
[Close]
BLAST of CF834625 vs. ExPASy Swiss-Prot
Match: METK2_DIACA (S-adenosylmethionine synthetase 2 OS=Dianthus caryophyllus GN=SAM2 PE=2 SV=1)

HSP 1 Score: 273.863 bits (699), Expect = 5.271e-73
Identity = 136/164 (82.93%), Postives = 143/164 (87.20%), Query Frame = -1 
Query:  163 FHLNPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 654
            FHLNPSGRFVIGGPHGDAGLTGRKIIIDTY             KDPTKVDRSGAYI RQAAKSIVASGLARRCIVQ+SYAIGVPEPLSVFVD+YGTGKI D+EILKIVKENFDFRPGMI+I LDLK+GGN R+LKTAAYGHFGR+DPDFTWE  K LKWEK QA
Sbjct:  234 FHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGAHGGGAFSRKDPTKVDRSGAYIARQAAKSIVASGLARRCIVQISYAIGVPEPLSVFVDTYGTGKIHDREILKIVKENFDFRPGMIAIALDLKKGGN-RYLKTAAYGHFGREDPDFTWEAAKTLKWEKPQA 396
The following BLAST results are available for this feature:
BLAST of CF834625 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK2_HORVU2.795e-7483.54S-adenosylmethionine synthetase 2 OS=Hordeum vulga... [more]
METK3_ATRNU6.226e-7484.15S-adenosylmethionine synthetase 3 OS=Atriplex numm... [more]
METK1_PEA6.226e-7485.63S-adenosylmethionine synthetase 1 (Fragment) OS=Pi... [more]
METK1_HORVU6.226e-7482.93S-adenosylmethionine synthetase 1 OS=Hordeum vulga... [more]
METK4_ATRNU8.131e-7484.15S-adenosylmethionine synthetase 4 OS=Atriplex numm... [more]
METK2_ATRNU8.131e-7484.15S-adenosylmethionine synthetase 2 OS=Atriplex numm... [more]
METK1_DAUCA8.131e-7469.81S-adenosylmethionine synthetase 1 OS=Daucus carota... [more]
METK1_ATRNU8.131e-7484.15S-adenosylmethionine synthetase 1 OS=Atriplex numm... [more]
METK_CAMSI5.271e-7388.82S-adenosylmethionine synthetase OS=Camellia sinens... [more]
METK2_DIACA5.271e-7382.93S-adenosylmethionine synthetase 2 OS=Dianthus cary... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_05N14_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa05N14, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF834625 ID=CF834625; Name=CF834625; organism=Citrus sinensis; type=EST; length=654bp
CCTTGGATTGAGAATCCATTAAACATATTTATTAAAAAAAAAAAGGAAAA
GAAAAAGAAAGAAAGAAAGAAAACATTCATGTAACTGAAACAGAAGTAAA
AAAGAAAAGTAGACATATATAAGCACCAATGAGAGGCCAGCAGCTCATCC
TTGATTAGTTTAAGCTTGAAGTTTCTCCCACTTGAGTGGCTTAACAATCT
CCCAAGTGAAATCAGGATCATCTCTGCCAAAGTGCCCATAAGCGGCAGTC
TTCAAGAACCTGCCGTTGCCACCCCTCTTGAGATCAAGATTAATAGAAAT
CATCCCAGGCCTAAAATCAAAATTCTCCTTCACAATCTTCAAAATTTCCT
TGTCAGGGATCTTCCCAGTCCCATAACTATCAACAAAAACTGACAAAGGC
TCGGGCACACCAATAGCGTAAGAAACCTGAACAATGCATCTCCTGGCAAG
CCCGCTTGCCACAATACTCTTAGCAGCTTGCCTTACAATGTAAGCACCGC
TTCTATCAACCTTGGTAGGATCTTTCCCTGAGAAAGCCCCACCACCGTGT
GCCCCCCAGCCACCATAAGTATCAATAATAATCTTCCGGCCGGTGAGCCC
AGCATCACCGTGAGGGCCACCAATCACAAAACGGCCAGATGGGTTAAGAT
GGAA
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