CF834867

Overview
NameCF834867
Unique NameCF834867
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length594
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig2387 contig Csv1_Contig2387:467..1060. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF834867 vs. ExPASy Swiss-Prot
Match: P5CR3_HUMAN (Pyrroline-5-carboxylate reductase 3 OS=Homo sapiens GN=PYCRL PE=1 SV=2)

HSP 1 Score: 75.0998 bits (183), Expect = 6.951e-18
Identity = 39/89 (43.82%), Postives = 52/89 (58.43%), Query Frame = -3 
Query:  137 LLGSGPAYIFXXXXXXXXXXXXXXLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAA 403
            L GSG A++               +P  LA  +A+QT+LG A M+   G+HP QL+ DV +PGGTTI G+H LE+ G R   M+AV AA
Sbjct:  176 LSGSGVAFVCAFSEALAEGAVKMGMPSSLAHRIAAQTLLGTAKMLLHEGQHPAQLRSDVCTPGGTTIYGLHALEQGGLRAATMSAVEAA 264

HSP 2 Score: 36.1946 bits (82), Expect = 6.951e-18
Identity = 20/60 (33.33%), Postives = 29/60 (48.33%), Query Frame = -2 
Query:  414 HSRFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 593
            ++R +RV+PN P  V E A VM+ G      +  L+  L  + G+     E   D  TGL
Sbjct:  117 NTRVLRVLPNLPCVVQEGAIVMARGRHVGSSETNLLQHLLEACGRCEEVPEAYVDIHTGL 176
[Close]
BLAST of CF834867 vs. ExPASy Swiss-Prot
Match: P5CR_AQUAE (Pyrroline-5-carboxylate reductase OS=Aquifex aeolicus GN=proC PE=3 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 4.369e-17
Identity = 33/86 (38.37%), Postives = 47/86 (54.65%), Query Frame = -3 
Query:  146 LLGSGPAYIFXXXXXXXXXXXXXXLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAV 403
            L GSGPA++F                 E AL +A  TV+G+A ++ +   +P +L   V SPGGTTI GI  LE+ GF+G +M  +
Sbjct:  170 LAGSGPAFVFSFIDALALAGVHQGFSYEQALRIALDTVMGSAKLLKEFQVNPNELIAKVTSPGGTTIEGIKYLEEKGFKGTVMECI 255

HSP 2 Score: 46.595 bits (109), Expect = 4.369e-17
Identity = 22/58 (37.93%), Postives = 34/58 (58.62%), Query Frame = -2 
Query:  414 RFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 587
            + +RVMPN   AVG     ++  G  +EE+   + +L  S G ++R +E+LFDA T L
Sbjct:  113 KIVRVMPNVNVAVGSGVMAITDNGNLSEEERSKVEELLLSCGTLYRIEERLFDAFTAL 170
[Close]
BLAST of CF834867 vs. ExPASy Swiss-Prot
Match: P5CR_CORML (Pyrroline-5-carboxylate reductase OS=Corynebacterium melassecola GN=proC PE=3 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 1.247e-16
Identity = 38/88 (43.18%), Postives = 49/88 (55.68%), Query Frame = -3 
Query:  134 GSGPAYIFXXXXXXXXXXXXXXLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAAA 397
            GS PAY+F              LPR  A  LA  +  GAA+M+ ++GK P +L+  V+SP GTT+A I ELE+SG RG    A  A A
Sbjct:  174 GSSPAYLFLVTEALIEAGVNLGLPRATAKKLAVASFEGAATMMKETGKEPSELRAGVSSPAGTTVAAIRELEESGIRGAFYRAAQACA 261

HSP 2 Score: 38.891 bits (89), Expect = 1.247e-16
Identity = 19/56 (33.93%), Postives = 29/56 (51.79%), Query Frame = -2 
Query:  414 IRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 581
            +RVMPNTP  VG+  + ++ G     E  E +  L  +VG +    E   DA+T +
Sbjct:  117 VRVMPNTPMLVGKGMSTVTKGRYVDAEQLEQVKDLLSTVGDVLEVAESDIDAVTAM 172
[Close]
BLAST of CF834867 vs. ExPASy Swiss-Prot
Match: P5CR_CORGL (Pyrroline-5-carboxylate reductase OS=Corynebacterium glutamicum GN=proC PE=3 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 1.247e-16
Identity = 38/88 (43.18%), Postives = 49/88 (55.68%), Query Frame = -3 
Query:  134 GSGPAYIFXXXXXXXXXXXXXXLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAAA 397
            GS PAY+F              LPR  A  LA  +  GAA+M+ ++GK P +L+  V+SP GTT+A I ELE+SG RG    A  A A
Sbjct:  174 GSSPAYLFLVTEALIEAGVNLGLPRATAKKLAVASFEGAATMMKETGKEPSELRAGVSSPAGTTVAAIRELEESGIRGAFYRAAQACA 261

HSP 2 Score: 38.891 bits (89), Expect = 1.247e-16
Identity = 19/56 (33.93%), Postives = 29/56 (51.79%), Query Frame = -2 
Query:  414 IRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 581
            +RVMPNTP  VG+  + ++ G     E  E +  L  +VG +    E   DA+T +
Sbjct:  117 VRVMPNTPMLVGKGMSTVTKGRYVDAEQLEQVKDLLSTVGDVLEVAESDIDAVTAM 172
[Close]
BLAST of CF834867 vs. ExPASy Swiss-Prot
Match: P5CR_THET2 (Pyrroline-5-carboxylate reductase OS=Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) GN=proC PE=3 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 3.564e-16
Identity = 35/86 (40.70%), Postives = 45/86 (52.33%), Query Frame = -3 
Query:  137 SGPAYIFXXXXXXXXXXXXXXLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAA 394
            S PAY+               +PR LAL LA+  +     ++   G+HP Q+KD+VASPGGTTI G+H LE    R     AV AA
Sbjct:  167 SAPAYLAVVAEALADAGVKMGMPRALALRLAADALAATGELL--KGRHPAQVKDEVASPGGTTIHGLHALEARAVRAAFYEAVEAA 250

HSP 2 Score: 43.1282 bits (100), Expect = 3.564e-16
Identity = 23/59 (38.98%), Postives = 33/59 (55.93%), Query Frame = -2 
Query:  414 RFIRVMPNTPSAVGEAATVMSLGGTATE-EDGELIGKLFGSVGKIWRADEKLFDAITGL 587
            R +R MPN    +GE++T ++    A E ED      LF +VG ++   E LFDA TG+
Sbjct:  106 RVVRAMPNLAVVIGESSTALTALKEAREAEDLAFARALFATVGDVYEIPEHLFDAFTGM 164
[Close]
BLAST of CF834867 vs. ExPASy Swiss-Prot
Match: P5CR_SHIFL (Pyrroline-5-carboxylate reductase OS=Shigella flexneri GN=proC PE=3 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 6.015e-16
Identity = 37/84 (44.05%), Postives = 50/84 (59.52%), Query Frame = -3 
Query:  146 GSGPAYIFXXXXXXXXXXXXXXLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAV 397
            GS PAY+F              +PR  A   A+Q V+G+A MV ++G+HPG LKD V SPGGTTI  +  LE+ GFR  ++ A+
Sbjct:  173 GSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLETGEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAM 256

HSP 2 Score: 30.0314 bits (66), Expect = 6.015e-16
Identity = 16/58 (27.59%), Postives = 26/58 (44.83%), Query Frame = -2 
Query:  414 RFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 587
            + IR MPNTP+ V    T ++     T ED   +  +F   G+     E +   + G+
Sbjct:  114 KIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVIAEPMIHPVVGV 171
[Close]
BLAST of CF834867 vs. ExPASy Swiss-Prot
Match: P5CR_ECOLI (Pyrroline-5-carboxylate reductase OS=Escherichia coli (strain K12) GN=proC PE=1 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 6.015e-16
Identity = 37/84 (44.05%), Postives = 50/84 (59.52%), Query Frame = -3 
Query:  146 GSGPAYIFXXXXXXXXXXXXXXLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAV 397
            GS PAY+F              +PR  A   A+Q V+G+A MV ++G+HPG LKD V SPGGTTI  +  LE+ GFR  ++ A+
Sbjct:  173 GSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLETGEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAM 256

HSP 2 Score: 30.0314 bits (66), Expect = 6.015e-16
Identity = 16/58 (27.59%), Postives = 26/58 (44.83%), Query Frame = -2 
Query:  414 RFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 587
            + IR MPNTP+ V    T ++     T ED   +  +F   G+     E +   + G+
Sbjct:  114 KIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVIAEPMIHPVVGV 171
[Close]
BLAST of CF834867 vs. ExPASy Swiss-Prot
Match: P5CR_YEAST (Pyrroline-5-carboxylate reductase OS=Saccharomyces cerevisiae GN=PRO3 PE=1 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 4.888e-15
Identity = 33/90 (36.67%), Postives = 48/90 (53.33%), Query Frame = -3 
Query:  134 LLGSGPAYIFXXXXXXXXXXXXXXLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAAA 403
            L+GSGPA++               +P + +   A + + G   MV KSG HP  LK  V +PGGTTIAG+  +E+ G +  ++N V  AA
Sbjct:  186 LVGSGPAFVLLMLESLMESGLKLGIPLQESKECAMKVLEGTVKMVEKSGAHPSVLKHQVCTPGGTTIAGLCVMEEKGVKSGIINGVEEAA 275

HSP 2 Score: 40.0466 bits (92), Expect = 4.888e-15
Identity = 22/59 (37.29%), Postives = 28/59 (47.46%), Query Frame = -2 
Query:  414 SRFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 590
            S   RVM NTP+  G    V+S     ++E   L+ +L   VGK     EK  DA T L
Sbjct:  128 STVCRVMTNTPAKYGYGCAVVSYSADVSKEQKPLVNELISQVGKYVELPEKNMDAATAL 186
[Close]
BLAST of CF834867 vs. ExPASy Swiss-Prot
Match: P5CR_SCHPO (Pyrroline-5-carboxylate reductase OS=Schizosaccharomyces pombe GN=pro3 PE=2 SV=1)

HSP 1 Score: 50.8322 bits (120), Expect = 2.354e-14
Identity = 28/87 (32.18%), Postives = 45/87 (51.72%), Query Frame = -3 
Query:  137 GSGPAYIFXXXXXXXXXXXXXXLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAA 397
            GSGPA++               +P   A  LA+QT++G   MV + G+HP  +++DV++P G TI+G+  LE    R  +   +  A
Sbjct:  188 GSGPAFVATMIEAMTDGGVMMGIPFPQAQELAAQTMVGTGRMVLQ-GQHPAMIRNDVSTPAGCTISGLLALEDGKIRSTIARGIEQA 273

HSP 2 Score: 48.521 bits (114), Expect = 2.354e-14
Identity = 24/59 (40.68%), Postives = 36/59 (61.02%), Query Frame = -2 
Query:  414 SRFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 590
            +R IR+MPNT S + E+ +V+  G  ATEED +    +F  +G+  +  EKL DA T +
Sbjct:  128 TRVIRIMPNTASRIRESMSVICPGPNATEEDIKFAEWVFNGIGRSMKLPEKLIDAATAV 186
[Close]
BLAST of CF834867 vs. ExPASy Swiss-Prot
Match: P5CR2_BACSU (Pyrroline-5-carboxylate reductase 2 OS=Bacillus subtilis GN=proI PE=3 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 3.059e-14
Identity = 32/90 (35.56%), Postives = 49/90 (54.44%), Query Frame = -3 
Query:  134 LLGSGPAYIFXXXXXXXXXXXXXXLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAAA 403
            + GSGPAY++              L +E A  L  QT+ GA  M+ +SGK P +L+ ++ SPGGTT AG+  L+ S F   +++ +   A
Sbjct:  170 IAGSGPAYVYRYIEAMEKAAQKVGLDKETAKALILQTMAGATDMLLQSGKQPEKLRKEITSPGGTTEAGLRALQDSRFEEAIIHCIEETA 259

HSP 2 Score: 36.5798 bits (83), Expect = 3.059e-14
Identity = 19/56 (33.93%), Postives = 32/56 (57.14%), Query Frame = -2 
Query:  414 IRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 581
            IRVMPNT +A+ ++AT  S+   A++ D      L  ++G     +E+  DA+T +
Sbjct:  115 IRVMPNTSAAIRKSATGFSVSTEASKNDIIAAKALLETIGDATLVEERHLDAVTAI 170
The following BLAST results are available for this feature:
BLAST of CF834867 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 35
Match NameE-valueIdentityDescription
P5CR3_HUMAN6.951e-1843.82Pyrroline-5-carboxylate reductase 3 OS=Homo sapien... [more]
P5CR_AQUAE4.369e-1738.37Pyrroline-5-carboxylate reductase OS=Aquifex aeoli... [more]
P5CR_CORML1.247e-1643.18Pyrroline-5-carboxylate reductase OS=Corynebacteri... [more]
P5CR_CORGL1.247e-1643.18Pyrroline-5-carboxylate reductase OS=Corynebacteri... [more]
P5CR_THET23.564e-1640.70Pyrroline-5-carboxylate reductase OS=Thermus therm... [more]
P5CR_SHIFL6.015e-1644.05Pyrroline-5-carboxylate reductase OS=Shigella flex... [more]
P5CR_ECOLI6.015e-1644.05Pyrroline-5-carboxylate reductase OS=Escherichia c... [more]
P5CR_YEAST4.888e-1536.67Pyrroline-5-carboxylate reductase OS=Saccharomyces... [more]
P5CR_SCHPO2.354e-1432.18Pyrroline-5-carboxylate reductase OS=Schizosacchar... [more]
P5CR2_BACSU3.059e-1435.56Pyrroline-5-carboxylate reductase 2 OS=Bacillus su... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_06E05_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa06E05, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF834867 ID=CF834867; Name=CF834867; organism=Citrus sinensis; type=EST; length=594bp
ATGCAACTGCCCCTTTCAATATCCATACAGAAAACAAAAAAAAGGAGAGG
GTTAATCGCCATACTAGAAGTGGAAGAGCTGAGAAACCGGAATCAAAATG
AATTAGCTCTAGGACAGTTCTCGGCTTCGCGTGAGCAGCAGCAACAACAG
CATTCATTAATATCCCACGAAATCCACTTTTCTCCAACTCATGAATACCA
GCAATAGTAGTCCCACCAGGCGATGCAACATCATCTTTTAGCTGACCAGG
ATGCTTCCCACTTTTAGTGACCATTGATGCTGCTCCTAATACTGTCTGAG
AAGCTAGACCAAGTGCAAGTTCTCGCGGTAGCCCTGCAGCCACAGCTGCA
TCAGCCAAAGCTTCAATTGCTAAAAATATATATGCTGGACCGCTGCCAAG
AAGCCACATACCTCAGGCCCGTGATTGCATCAAACAATTTCTCATCCGCT
CGCCATATCTTGCCAACTGATCCGAATAATTTACCGATTAGTTCCCCATC
CTCTTCTGTTGCAGTCCCTCCCAAGCTCATAACTGTTGCAGCCTCACCTA
CAGCAGAAGGTGTATTAGGCATAACTCTAATAAACCGGCTGTGA
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