CF835058

Overview
NameCF835058
Unique NameCF835058
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length670
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF835058 vs. ExPASy Swiss-Prot
Match: NEDD8_CAEEL (NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 9.125e-20
Identity = 46/76 (60.53%), Postives = 58/76 (76.32%), Query Frame = -2
Query:  409 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 636
            M I VKTLTGK I L++E +D ++ +K K+++KEGIPP QQRLIFAGKQ+ D +T ADY +   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLGGSVLHLVLALRGG 76          
BLAST of CF835058 vs. ExPASy Swiss-Prot
Match: RUB2_ARATH (NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN=RUB2 PE=2 SV=2)

HSP 1 Score: 95.9005 bits (237), Expect = 2.033e-19
Identity = 46/74 (62.16%), Postives = 58/74 (78.38%), Query Frame = -2
Query:  409 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 630
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGG 76          
BLAST of CF835058 vs. ExPASy Swiss-Prot
Match: RS27A_DAUCA (40S ribosomal protein S27a (Fragment) OS=Daucus carota PE=3 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 2.033e-19
Identity = 42/44 (95.45%), Postives = 43/44 (97.73%), Query Frame = -2
Query:  208 LAVLQFYKVDDSGKVQRLRKECPNAECGAGTFMANHFDRHYCGK 339
            LAVLQFYKVD+SGKVQRLRKECPN ECGAGTFMANHFDRHYCGK
Sbjct:   24 LAVLQFYKVDESGKVQRLRKECPNGECGAGTFMANHFDRHYCGK 67          
BLAST of CF835058 vs. ExPASy Swiss-Prot
Match: RS27A_CHICK (40S ribosomal protein S27a OS=Gallus gallus GN=RPS27A PE=3 SV=2)

HSP 1 Score: 93.5893 bits (231), Expect = 1.009e-18
Identity = 38/53 (71.70%), Postives = 47/53 (88.68%), Query Frame = -2
Query:  181 LAVLQFYKVDDSGKVQRLRKECPNAECGAGTFMANHFDRHYCGKCGLTYVYQK 339
            LAVL++YKVD++GK+ RLR+ECP+ ECGAG FMA+HFDRHYCGKC LTY + K
Sbjct:   24 LAVLKYYKVDENGKISRLRRECPSEECGAGVFMASHFDRHYCGKCCLTYCFNK 76          
BLAST of CF835058 vs. ExPASy Swiss-Prot
Match: RS27A_HUMAN (40S ribosomal protein S27a OS=Homo sapiens GN=RPS27A PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.318e-18
Identity = 38/53 (71.70%), Postives = 47/53 (88.68%), Query Frame = -2
Query:  181 LAVLQFYKVDDSGKVQRLRKECPNAECGAGTFMANHFDRHYCGKCGLTYVYQK 339
            LAVL++YKVD++GK+ RLR+ECP+ ECGAG FMA+HFDRHYCGKC LTY + K
Sbjct:   24 LAVLKYYKVDENGKISRLRRECPSDECGAGVFMASHFDRHYCGKCCLTYCFNK 76          
BLAST of CF835058 vs. ExPASy Swiss-Prot
Match: RS27A_CAVPO (40S ribosomal protein S27a OS=Cavia porcellus GN=RPS27A PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.318e-18
Identity = 38/53 (71.70%), Postives = 47/53 (88.68%), Query Frame = -2
Query:  181 LAVLQFYKVDDSGKVQRLRKECPNAECGAGTFMANHFDRHYCGKCGLTYVYQK 339
            LAVL++YKVD++GK+ RLR+ECP+ ECGAG FMA+HFDRHYCGKC LTY + K
Sbjct:   24 LAVLKYYKVDENGKISRLRRECPSDECGAGVFMASHFDRHYCGKCCLTYCFNK 76          
BLAST of CF835058 vs. ExPASy Swiss-Prot
Match: RS27A_BOVIN (40S ribosomal protein S27a OS=Bos taurus GN=RPS27A PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.318e-18
Identity = 38/53 (71.70%), Postives = 47/53 (88.68%), Query Frame = -2
Query:  181 LAVLQFYKVDDSGKVQRLRKECPNAECGAGTFMANHFDRHYCGKCGLTYVYQK 339
            LAVL++YKVD++GK+ RLR+ECP+ ECGAG FMA+HFDRHYCGKC LTY + K
Sbjct:   24 LAVLKYYKVDENGKISRLRRECPSDECGAGVFMASHFDRHYCGKCCLTYCFNK 76          
BLAST of CF835058 vs. ExPASy Swiss-Prot
Match: NEDD8_RAT (NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.318e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -2
Query:  409 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 636
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76          
BLAST of CF835058 vs. ExPASy Swiss-Prot
Match: NEDD8_RABIT (NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.318e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -2
Query:  409 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 636
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76          
BLAST of CF835058 vs. ExPASy Swiss-Prot
Match: NEDD8_MOUSE (NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2)

HSP 1 Score: 93.2041 bits (230), Expect = 1.318e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -2
Query:  409 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 636
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76          
The following BLAST results are available for this feature:
BLAST of CF835058 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 123
Match NameE-valueIdentityDescription
NEDD8_CAEEL9.125e-2060.53NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1[more]
RUB2_ARATH2.033e-1962.16NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN... [more]
RS27A_DAUCA2.033e-1995.4540S ribosomal protein S27a (Fragment) OS=Daucus ca... [more]
RS27A_CHICK1.009e-1871.7040S ribosomal protein S27a OS=Gallus gallus GN=RPS... [more]
RS27A_HUMAN1.318e-1871.7040S ribosomal protein S27a OS=Homo sapiens GN=RPS2... [more]
RS27A_CAVPO1.318e-1871.7040S ribosomal protein S27a OS=Cavia porcellus GN=R... [more]
RS27A_BOVIN1.318e-1871.7040S ribosomal protein S27a OS=Bos taurus GN=RPS27A... [more]
NEDD8_RAT1.318e-1856.58NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1[more]
NEDD8_RABIT1.318e-1856.58NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1[more]
NEDD8_MOUSE1.318e-1856.58NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2[more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_06J16_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa06J16, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF835058 ID=CF835058; Name=CF835058; organism=Citrus sinensis; type=EST; length=670bp
TTTTTTTTTTTTTTTAAATTTAAAACAGATAACCATTTAATTAAACCTGT
TCAAGCAAACAAGTATATAGTCAAACAAAATTACGAAAGAGTCATAATTT
TTGAACCACAAAAACCATAGTAATTAATACTAATTAAAAATAGCAAAACA
ACCACCTAATCTCGATCAATCACCGCCAGCCTTCTGGTAAACATAGGTGA
GTCCACACTTGCCGCAGTAGTGCCTGTCAAAGTGGTTAGCCATGAAGGTG
CCGGCTCCACACTCAGCATTCGGACACTCCTTCCTCAGCCTCTGGACCTT
TCCAGAATCATCGACCTTGTAAAACTGCAAGACGGCGAGCTTGACCTTCT
TCTTCTTGTGCTTGATCTTTTTCGGCTTGGTGTAGGTCTTCTTCTTACGC
TTCTTCGCGCCACCACGGAGACGGAGGACGAGGTGGAGTGTTGACTCCTT
TTGGATGTTGTAGTCGGCTAGGGTTCTGCCGTCCTCGAGCTGCTTTCCGG
CGAAGATGAGACGCTGCTGGTCCGGTGGAATCCCCTCCTTGTCCTGGATC
TTGGCCTTGACATTGTCGATGGTGTCCGATGATTCGACCTCTAGGGTTAT
GGTCTTTCCCGTTAGGGTTTTCACGAAGATCTGCATGGCTGCAGATGAGG
AGAGACCCAGCAAATTTCCC
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