CF835073

Overview
NameCF835073
Unique NameCF835073
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length706
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDIA1_PONAB (Protein disulfide-isomerase OS=Pongo abelii GN=P4HB PE=2 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 1.036e-24
Identity = 69/225 (30.67%), Postives = 123/225 (54.67%), Query Frame = -3
Query:   63 RFMFTAVDIADEDLAKPFLTLFGLEESKNTVVTAFD-NKAISKFLLESD-LTPSNIEEFCSRLLHGTLTPYLKSQPIPDNTNAN-VQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANEHPKLQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIK----------EQLKEKDQSPKDEQLKERDQ-APKDEL 695
            + +F  +D +D    +  L  FGL++ +   V      + ++K+  ES+ LT   I EFC R L G + P+L SQ +PD+ +   V+++VGK F+D+  +  K+V +E Y PWC  C+  +   +KL + +K  +N+VIAK+D++ANE   ++V  +PTL F+PA      I  +   +      F++          + L++ +++ + +  ++ DQ A KDEL
Sbjct:  285 KILFIFID-SDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESEELTAERITEFCHRFLEGKIKPHLMSQELPDDWDKQPVKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQDGAGDDDDLEDLEEAEEPDMEEDDDQKAVKDEL 508          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDIA3_CHICK (Protein disulfide-isomerase A3 OS=Gallus gallus GN=PDIA3 PE=2 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 1.353e-24
Identity = 63/196 (32.14%), Postives = 106/196 (54.08%), Query Frame = -3
Query:   84 LTLFGLEESKNTV-VTAFDNKAISKFLLESDLTPSN--IEEFCSRLLHGTLTPYLKSQPIPDNTNANVQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANEHPK-LQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIKEQ------LKEKDQSPKDEQLKERD 641
            L+ FGL+ S     V A       KF+++ + +     +E F      G L  YLKS+P+P+N +  V++VV + FD++V    KDVL+E Y PWC  C+    + ++L +      N+VIAK+DA+AN+ P   +V  +PT+ F PAG K +P K        +  +++K +      L+E+D++ K ++  + D
Sbjct:  309 LSEFGLDNSVGEAPVVAIRTAKGDKFVMQEEFSRDGKALERFLQDYFDGNLKKYLKSEPVPENNDGPVKVVVAENFDEIVNAEDKDVLIEFYAPWCGHCKNLEPKYKELGEKLSKDPNIVIAKMDATANDVPSPYEVRGFPTIYFAPAGKKQSPKKYEGGREVSDFISYLKREATSTPVLQEEDKAKKSKKKAKED 504          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDIA1_MACFU (Protein disulfide-isomerase OS=Macaca fuscata fuscata GN=P4HB PE=2 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 1.768e-24
Identity = 68/225 (30.22%), Postives = 124/225 (55.11%), Query Frame = -3
Query:   63 RFMFTAVDIADEDLAKPFLTLFGLEESKNTVVTAFD-NKAISKFLLESD-LTPSNIEEFCSRLLHGTLTPYLKSQPIPDNTNAN-VQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANEHPKLQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIK----------EQLKEKDQSPKDEQLKERDQ-APKDEL 695
            + +F  +D +D    +  L  FGL++ +   V      + ++K+  ESD LT   I EFC R L G + P+L SQ +P++ +   V+++VGK F+++  + +K+V +E Y PWC  C+  +   +KL + +K  +N+VIAK+D++ANE   ++V  +PTL F+PA      I  +   +      F++          + L++ +++ + +  ++ DQ A KDEL
Sbjct:  287 KILFIFID-SDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESDELTAERITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEEVAFDENKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAIKVHSFPTLKFFPASVDRTVIDYNGERTLDGFKKFLESGGQDGAGDDDDLEDLEEAEEPDMEEDDDQKAVKDEL 510          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDI2_DICDI (Protein disulfide-isomerase 2 OS=Dictyostelium discoideum GN=pdi2 PE=3 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 2.309e-24
Identity = 61/187 (32.62%), Postives = 105/187 (56.15%), Query Frame = -3
Query:   63 VTAFDNKAISKFLLESDLTPSNIEEFCSRLLHGTLTPYLKSQPIPDNTNANVQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANEHPK-LQVEEYPTLLFYPAGDKANPIKVSA-RSSSKNIAAFIKEQLKEKDQSPKDE-----QLKERDQAPKDEL 602
            V +  NKA   F  +   +   + ++   ++ G ++P++KSQP P++ +A V++ VG TF  LVL+S KDVL+E Y PWC  C+  +   +KL ++ K ++++ I KIDA +N+ P  +++  YPT++ + A DK NPI     R+   N   FI++    + + P  +     + K+   A  DEL
Sbjct:  327 VDSVANKARYNFDEKETFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFYAPWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKENPISYEGQRNDHMNFVEFIQDNAAIEFKLPSSQTDDNVESKKDSSAKHDEL 513          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDIA1_HUMAN (Protein disulfide-isomerase OS=Homo sapiens GN=P4HB PE=1 SV=3)

HSP 1 Score: 112.079 bits (279), Expect = 3.015e-24
Identity = 68/225 (30.22%), Postives = 123/225 (54.67%), Query Frame = -3
Query:   63 RFMFTAVDIADEDLAKPFLTLFGLEESKNTVVTAFD-NKAISKFLLESD-LTPSNIEEFCSRLLHGTLTPYLKSQPIPDNTNAN-VQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANEHPKLQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIK----------EQLKEKDQSPKDEQLKERDQ-APKDEL 695
            + +F  +D +D    +  L  FGL++ +   V      + ++K+  ES+ LT   I EFC R L G + P+L SQ +P++ +   V+++VGK F+D+  +  K+V +E Y PWC  C+  +   +KL + +K  +N+VIAK+D++ANE   ++V  +PTL F+PA      I  +   +      F++          + L++ +++ + +  ++ DQ A KDEL
Sbjct:  285 KILFIFID-SDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESEELTAERITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQDGAGDDDDLEDLEEAEEPDMEEDDDQKAVKDEL 508          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDIA1_RABIT (Protein disulfide-isomerase OS=Oryctolagus cuniculus GN=P4HB PE=2 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 5.143e-24
Identity = 69/223 (30.94%), Postives = 115/223 (51.57%), Query Frame = -3
Query:   81 FMFTAVDIADEDLAKPFLTLFGLEESKNTVVTAFD-NKAISKFLLESD-LTPSNIEEFCSRLLHGTLTPYLKSQPIPDNTNAN-VQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANEHPKLQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIK----------------EQLKEKDQSPKDEQLKERDQ 692
            F+F   D AD    +  L  FGL++ +   V      + ++K+  ESD LT   I EFC R L G + P+L SQ +P++ +   V+++VGK F+++  +  K+V +E Y PWC  C+  +   +KL + +K   ++VIAK+D++ANE   ++V  +PTL F+PAG     I  +   +      F++                E+ +E D    D+Q   RD+
Sbjct:  289 FIFIDSDHADN---QRILEFFGLKKEECPAVRLITLEEEMTKYKPESDELTAEGITEFCQRFLEGKIKPHLMSQELPEDWDRQPVKVLVGKNFEEVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKEHQDIVIAKMDSTANEVEAVKVHSFPTLKFFPAGPGRTVIDYNGERTLDGFKKFLESGGQDGAGDEDGLEDLEEAEEPDLEEDDDQKAVRDE 508          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDIA3_MOUSE (Protein disulfide-isomerase A3 OS=Mus musculus GN=Pdia3 PE=1 SV=2)

HSP 1 Score: 110.923 bits (276), Expect = 6.717e-24
Identity = 66/197 (33.50%), Postives = 106/197 (53.81%), Query Frame = -3
Query:   69 LTLFGLEESKNTV-VTAFDNKAISKFLLESDLTPSN--IEEFCSRLLHGTLTPYLKSQPIPDNTNANVQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANEHPK-LQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIKEQLKEKDQSP--KDEQLKERDQAPKD 641
            L+ FGLE +   V V A       KF+++ + +     +E+F      G L  YLKS+PIP++    V++VV + FDD+V    KDVL+E Y PWC  C+    + ++L +      N+VIAK+DA+AN+ P   +V+ +PT+ F PA  K  P K       + +  FI    +E    P  ++E+ K++ +A +D
Sbjct:  311 LSDFGLESTTGEVPVVAIRTAKGEKFVMQEEFSRDGKALEQFLQEYFDGNLKRYLKSEPIPESNEGPVKVVVAENFDDIVNEEDKDVLIEFYAPWCGHCKNLEPKYKELGEKLSKDPNIVIAKMDATANDVPSPYEVKGFPTIYFSPANKKLTPKKY---EGGRELNDFISYLQREATNPPIIQEEKPKKKKKAQED 504          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDI1_SCHPO (Putative protein disulfide-isomerase C1F5.02 OS=Schizosaccharomyces pombe GN=SPAC1F5.02 PE=2 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 1.496e-23
Identity = 58/171 (33.92%), Postives = 100/171 (58.48%), Query Frame = -3
Query:   63 SDLTPSNIEEFCSRLLHGTLTPYLKSQPIPDNTNANVQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANEHPKLQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIKEQL------KEKDQSPKDEQLKERDQAPKDEL 557
            ++LT   + +F    + G L P +KSQPIP++   ++ ++V   FDD+V++  KDVL+E Y PWC  C+  +   EKLA+ +    N+V+AKIDA+ N+   + +  +PT++F+ A DK NP++     + ++++AFI +        KEK+  P  +   E   A +DE+
Sbjct:  322 TELTAKAMTKFVGDFVDGKLQPKIKSQPIPESQE-DLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEYSDDSNVVVAKIDATEND-ISVSISGFPTIMFFKANDKVNPVRYEGDRTLEDLSAFIDKHASFEPIKKEKESVPAPD--LEDQVAVEDEM 488          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDI1_ARATH (Probable protein disulfide-isomerase 1 OS=Arabidopsis thaliana GN=At1g21750 PE=1 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 1.496e-23
Identity = 66/205 (32.20%), Postives = 112/205 (54.63%), Query Frame = -3
Query:   63 IADEDLAKPFLTLFGLEESKNTVVTAFDNKAISKFLLESDLTPSNIEEFCSRLLHGTLTPYLKSQPIPDNTNANVQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANEHPK--LQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIKEQLKEKDQSPKDEQLKERDQAPKDEL 671
            + D + ++     FGLEES+  ++      A  K  L++++    IE +      G + P+ KSQPIP   N  V++VV  + DD+VLNS K+VLLE Y PWC  C+  +  ++++A  ++   ++VIAK+DA+AN+ PK    V+ +PT+ F  A    N +      + ++  +F+    K KD   + ++ +E  +  KDEL
Sbjct:  304 LGDAENSQGAFQYFGLEESQVPLIII--QTADDKKYLKTNVEVDQIESWVKDFKDGKIAPHKKSQPIPAENNEPVKVVVSDSLDDIVLNSGKNVLLEFYAPWCGHCQKLAPILDEVAVSYQSDSSVVIAKLDATANDFPKDTFDVKGFPTIYFKSA--SGNVVVYEGDRTKEDFISFVD---KNKDTVGEPKKEEETTEEVKDEL 501          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDIA3_RAT (Protein disulfide-isomerase A3 OS=Rattus norvegicus GN=Pdia3 PE=1 SV=2)

HSP 1 Score: 108.997 bits (271), Expect = 2.552e-23
Identity = 65/197 (32.99%), Postives = 105/197 (53.30%), Query Frame = -3
Query:   69 LTLFGLEESKNTV-VTAFDNKAISKFLLESDLTPSN--IEEFCSRLLHGTLTPYLKSQPIPDNTNANVQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANEHPK-LQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIKEQLKEKDQSP--KDEQLKERDQAPKD 641
            L+ FGLE +   + V A       KF+++ + +     +E F      G L  YLKS+PIP+     V++VV ++FDD+V    KDVL+E Y PWC  C+    + ++L +      N+VIAK+DA+AN+ P   +V+ +PT+ F PA  K  P K       + +  FI    +E    P  ++E+ K++ +A +D
Sbjct:  311 LSDFGLESTTGEIPVVAIRTAKGEKFVMQEEFSRDGKALERFLQEYFDGNLKRYLKSEPIPETNEGPVKVVVAESFDDIVNAEDKDVLIEFYAPWCGHCKNLEPKYKELGEKLSKDPNIVIAKMDATANDVPSPYEVKGFPTIYFSPANKKLTPKKY---EGGRELNDFISYLQREATNPPIIQEEKPKKKKKAQED 504          
The following BLAST results are available for this feature:
BLAST of CF835073 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 51
Match NameE-valueIdentityDescription
PDIA1_PONAB1.036e-2430.67Protein disulfide-isomerase OS=Pongo abelii GN=P4H... [more]
PDIA3_CHICK1.353e-2432.14Protein disulfide-isomerase A3 OS=Gallus gallus GN... [more]
PDIA1_MACFU1.768e-2430.22Protein disulfide-isomerase OS=Macaca fuscata fusc... [more]
PDI2_DICDI2.309e-2432.62Protein disulfide-isomerase 2 OS=Dictyostelium dis... [more]
PDIA1_HUMAN3.015e-2430.22Protein disulfide-isomerase OS=Homo sapiens GN=P4H... [more]
PDIA1_RABIT5.143e-2430.94Protein disulfide-isomerase OS=Oryctolagus cunicul... [more]
PDIA3_MOUSE6.717e-2433.50Protein disulfide-isomerase A3 OS=Mus musculus GN=... [more]
PDI1_SCHPO1.496e-2333.92Putative protein disulfide-isomerase C1F5.02 OS=Sc... [more]
PDI1_ARATH1.496e-2332.20Probable protein disulfide-isomerase 1 OS=Arabidop... [more]
PDIA3_RAT2.552e-2332.99Protein disulfide-isomerase A3 OS=Rattus norvegicu... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_06J24_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa06J24, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF835073 ID=CF835073; Name=CF835073; organism=Citrus sinensis; type=EST; length=706bp
TTTTTTTTTTTTTTTCCTTTTTCTGTTAAAGTTTCAATATTCCACCCCTT
TTATAAGATCTATAATTCATCTTTGGGAGCTTGATCCCTTTCTTTCAATT
GTTCATCTTTTGGGGATTGATCTTTTTCTTTCAATTGTTCCTTGATAAAT
GCTGCTATGTTCTTTGAGCTGGACCTTGCTGAAACTTTGATCGGATTTGC
TTTATCACCAGCTGGGTAGAATAAAAGTGTTGGGTACTCTTCAACCTGCA
GTTTTGGATGCTCATTTGCAGAAGCATCTATCTTTGCAATGACTAGATTG
TCCAAACCCTTGAAGTGCTTAGCCAGCTTTTCGATTTGCTTGCTAGTTGT
CTCACATCTGACACACCATGGTGTATATACCTCTAGAAGAACATCCTTGT
GACTGTTCAAAACCAAGTCGTCAAATGTCTTTCCCACAACAATCTGCACG
TTTGCGTTTGTGTTATCTGGTATTGGCTGTGATTTCAAGTAAGGCGTCAG
AGTACCATGCAAAAGCCTTGAGCAAAACTCTTCTATGTTGCTTGGCGTGA
GATCTGACTCCAACAAAAACTTTGAAATGGCTTTGTTATCAAAGGCAGTC
ACCACAGTGTTCTTTGATTCTTCAAGCCCAAATAAAGTTAAAAATGGCTT
CGCCAGGTCTTCATCTGCGATATCTACTGCTGTAAACATGAATCTTTCCC
TTGAAA
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