CF835073

Overview
NameCF835073
Unique NameCF835073
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length706
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDIA1_CRIGR (Protein disulfide-isomerase OS=Cricetulus griseus GN=P4HB PE=2 SV=1)

HSP 1 Score: 108.997 bits (271), Expect = 2.552e-23
Identity = 69/229 (30.13%), Postives = 118/229 (51.53%), Query Frame = -3
Query:   63 RFMFTAVDIADEDLAKPFLTLFGLEESKNTVVTAFD-NKAISKFLLESD-LTPSNIEEFCSRLLHGTLTPYLKSQPIPDNTNAN-VQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANEHPKLQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIK---------------EQLKEKDQSPKDEQLKERDQAPKDEL 695
            + +F  +D +D    +  L  FGL++ +   V      + ++K+  ESD LT   I EFC R L G + P+L SQ +P++ +   V+++VGK F+++  +  K+V +E Y PWC  C+  +   +KL + +K  +N++IAK+D++ANE   ++V  +PTL F+PA      I  +   +      F++               E+  E D    D+Q     +A KDEL
Sbjct:  287 KILFIFID-SDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESDELTAEKITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEEVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIIIAKMDSTANEVEAVKVHSFPTLKFFPATADRTVIDYNGERTLDGFKKFLESGGQDGAGDDDDVDLEEALEPDMEEDDDQ-----KAVKDEL 509          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDIA1_MOUSE (Protein disulfide-isomerase OS=Mus musculus GN=P4hb PE=1 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 4.354e-23
Identity = 66/224 (29.46%), Postives = 117/224 (52.23%), Query Frame = -3
Query:   63 RFMFTAVDIADEDLAKPFLTLFGLEESKNTVVTAFD-NKAISKFLLESD-LTPSNIEEFCSRLLHGTLTPYLKSQPIPDNTNAN-VQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANEHPKLQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIK----------EQLKEKDQSPKDEQLKERDQAPKDEL 695
            + +F  +D +D    +  L  FGL++ +   V      + ++K+  ESD LT   I EFC R L G + P+L SQ +P++ +   V+++VG  F+++  +  K+V +E Y PWC  C+  +   +KL + +K  +N++IAK+D++ANE   ++V  +PTL F+PA      I  +   +      F++          E L  ++    D +  +  +A KDEL
Sbjct:  287 KILFIFID-SDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESDELTAEKITEFCHRFLEGKIKPHLMSQEVPEDWDKQPVKVLVGANFEEVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIIIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQDGAGDDEDLDLEEALEPDMEEDDDQKAVKDEL 509          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDI_HUMIN (Protein disulfide-isomerase OS=Humicola insolens PE=1 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 9.699e-23
Identity = 65/195 (33.33%), Postives = 103/195 (52.82%), Query Frame = -3
Query:   99 AKPFLTLFGLEESKNTVVTAFDNKAISK-----FLLESDLTPSNIEEFCSRLLHGTLTPYLKSQPIPDNTNANVQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLA-----KHFKGLDNLVIAKIDASANEHPKLQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIKEQLKEKDQSPKDEQ 653
            AK F    G    K     AF  + ++K     F  E ++T   I+ F    + G + P +KS+PIP+     V +VV K ++++VL+  KDVL+E Y PWC  C+  + + E+L        FK  D +VIAK+DA+AN+ P  +++ +PT+  YPAG K  P+  S   + +++  FI E  K K    +D +
Sbjct:  284 AKAFGAHAGNLNLKTDKFPAFAIQEVAKNQKFPFDQEKEITFEAIKAFVDDFVAGKIEPSIKSEPIPEKQEGPVTVVVAKNYNEIVLDDTKDVLIEFYAPWCGHCKALAPKYEELGALYAKSEFK--DRVVIAKVDATANDVPD-EIQGFPTIKLYPAGAKGQPVTYSGSRTVEDLIKFIAENGKYKAAISEDAE 475          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDI_ASPNG (Protein disulfide-isomerase OS=Aspergillus niger GN=pdiA PE=2 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 9.699e-23
Identity = 55/146 (37.67%), Postives = 89/146 (60.96%), Query Frame = -3
Query:  129 DLTPSNIEEFCSRLLHGTLTPYLKSQPIPDNTNANVQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNL----VIAKIDASANEHPKLQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIKEQLK 554
            +L    +E+F   +L G + P +KS+P+P++    V +VV  ++ DLV+++ KDVLLE Y PWC  C+  + + ++LA  +    +L     IAKIDA+AN+ P   +  +PTL  YPAG K +PI+ S   + +++A F+KE  K
Sbjct:  326 ELNADEVEKFIQDVLDGKVEPSIKSEPVPESQEGPVTVVVAHSYKDLVIDNDKDVLLEFYAPWCGHCKALAPKYDELAALYADHPDLAAKVTIAKIDATANDVPD-PITGFPTLRLYPAGAKDSPIEYSGSRTVEDLANFVKENGK 470          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDIA1_RAT (Protein disulfide-isomerase OS=Rattus norvegicus GN=P4hb PE=1 SV=2)

HSP 1 Score: 107.071 bits (266), Expect = 9.699e-23
Identity = 68/229 (29.69%), Postives = 117/229 (51.09%), Query Frame = -3
Query:   63 RFMFTAVDIADEDLAKPFLTLFGLEESKNTVVTAFD-NKAISKFLLESD-LTPSNIEEFCSRLLHGTLTPYLKSQPIPDNTNAN-VQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANEHPKLQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIK---------------EQLKEKDQSPKDEQLKERDQAPKDEL 695
            + +F  +D +D    +  L  FGL++ +   V      + ++K+  ESD LT   I +FC   L G + P+L SQ +P++ +   V+++VGK F+++  +  K+V +E Y PWC  C+  +   +KL + +K  +N+VIAK+D++ANE   ++V  +PTL F+PA      I  +   +      F++               E+  E D    D+Q     +A KDEL
Sbjct:  287 KILFIFID-SDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESDELTAEKITQFCHHFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEEVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQDGAGDNDDLDLEEALEPDMEEDDDQ-----KAVKDEL 509          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDI_ASPOR (Protein disulfide-isomerase OS=Aspergillus oryzae GN=pdiA PE=3 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 1.267e-22
Identity = 54/163 (33.13%), Postives = 94/163 (57.67%), Query Frame = -3
Query:   66 DLTPSNIEEFCSRLLHGTLTPYLKSQPIPDNTNANVQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANEHPKLQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIKEQLKEKDQSPKDEQLKERDQAPKDE 554
            ++   +I +F   +L   + P +KS+ IP+     V +VV  ++ DLVL++ KDVLLE Y PWC  C+  + + E+LA  +K +  + IAKIDA+AN+ P   +  +PT+  + AG K +P++     + +++A F+KE  K K  + + +  KE++     E
Sbjct:  330 EVKAKDIGKFIQDVLDDKVEPSIKSEAIPETQEGPVTVVVAHSYKDLVLDNEKDVLLEFYAPWCGHCKALAPKYEELASLYKDIPEVTIAKIDATANDVPD-SITGFPTIKLFAAGAKDSPVEYEGSRTVEDLANFVKENGKHKVDALEVDPKKEQESGDATE 491          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDI_MEDSA (Protein disulfide-isomerase OS=Medicago sativa GN=PDI PE=2 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 3.686e-22
Identity = 70/215 (32.56%), Postives = 117/215 (54.42%), Query Frame = -3
Query:   63 IADEDLAKPFLTLFGLEESKNT--VVTAFDNKAISKFLLESDLTPSNIEEFCSRLLHGTLTPYLKSQPIPDNTNANVQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANEHP--KLQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIKEQLKEKDQSPKDEQLKERDQA--------PKDEL 671
            + D + ++     FGL+E +    ++   D K   K  LE D  P+ ++ +      G + P++KS+PIP+  N  V++VVG+T +D+V  S K+VL+E Y PWC  C+  +  ++++A  F+   ++VIAK+DA+AN+ P     V+ YPTL F  A  K +  +     + ++I  FI+   K KD++    Q  E+ +A        PKDEL
Sbjct:  307 VGDVESSQGAFQYFGLKEEQVPLIIIQHNDGKKFFKPNLELDQLPTWLKAYKD----GKVEPFVKSEPIPETNNEPVKVVVGQTLEDVVFKSGKNVLIEFYAPWCGHCKQLAPILDEVAVSFQSDADVVIAKLDATANDIPTDTFDVQGYPTLYFRSASGKLS--QYDGGRTKEDIIEFIE---KNKDKTGAAHQEVEQPKAAAQPEAEQPKDEL 512          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDI_DATGL (Protein disulfide-isomerase OS=Datisca glomerata GN=PDI PE=2 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 1.072e-21
Identity = 60/203 (29.56%), Postives = 110/203 (54.19%), Query Frame = -3
Query:   69 IADEDLAKPFLTLFGLEESKNTVVTAFDNKAISKFLLESDLTPSNIEEFCSRLLHGTLTPYLKSQPIPDNTNANVQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANEHP--KLQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIKEQLKEKDQSPKDEQLKERDQAPKD 671
            I D + ++     FGL + +  ++   +N       L+ +L P +I  +        L+PY KS+PIP++ N  V++VV  + D++V  S K+VLLE Y PWC  C+  +  ++++A  F+   +++IAK+DA+AN++P     V+ YPTL F  A  +   ++     + ++   FI+   K +++S K E + + DQ   +
Sbjct:  307 IGDTESSQGAFQYFGLRDDQVPLIVIQNNDGTK--YLKPNLEPDHIASWVKEYKDCKLSPYRKSEPIPEHNNEPVKVVVADSLDEIVFKSGKNVLLEFYAPWCGHCKQLAPILDEVAVSFENDPDVLIAKLDATANDYPTNTFDVKGYPTLYFKSASGEL--LQYDGGRTKEDFIEFIE---KNREKSSKKESIVKDDQTDSE 502          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDIA4_CAEEL (Probable protein disulfide-isomerase A4 OS=Caenorhabditis elegans GN=C14B9.2 PE=2 SV=2)

HSP 1 Score: 103.605 bits (257), Expect = 1.072e-21
Identity = 70/206 (33.98%), Postives = 107/206 (51.94%), Query Frame = -3
Query:  102 RERFMFTAVDIADEDLAKPFLTLFGLEES---KNTVVTAFDNKAISKFLLESD-LTPSNIEEFCSRLLHGTLTPYLKSQPIPDNTNANVQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLD-NLVIAKIDASANEHP-KLQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIKEQLKEKDQSPKDE 701
            ++++ F   D  +E+ AK    L GL +S    N VV  +D K       E D     N+E F  ++  G    ++KS P P +    V+ VVG  FD +V +  KDVL+E Y PWC  C++   +  +LA+  K    N+V+AK+DA+ N+ P +  VE +PT+ F PAG K+ PIK S     +++  F+ +    K    KDE
Sbjct:  416 KDKYKFAVAD--EEEFAKELEEL-GLGDSGLEHNVVVFGYDGKKYPMNPDEFDGELDENLEAFMKQISSGKAKAHVKSAPAPKDDKGPVKTVVGSNFDKIVNDESKDVLIEFYAPWCGHCKSFESKYVELAQALKKTQPNVVLAKMDATINDAPSQFAVEGFPTIYFAPAGKKSEPIKYSGNRDLEDLKKFMTKH-GVKSFQKKDE 617          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDI2_ARATH (Protein disulfide-isomerase 2 OS=Arabidopsis thaliana GN=At1g77510 PE=1 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 1.401e-21
Identity = 66/213 (30.99%), Postives = 115/213 (53.99%), Query Frame = -3
Query:   63 IADEDLAKPFLTLFGLEESKNT--VVTAFDNKAISKFLLESDLTPSNIEEFCSRLLHGTLTPYLKSQPIPDNTNANVQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANEHPK--LQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIKEQLKEK-----DQSPKDEQLKERDQ-APKDEL 671
            + D + ++     FGLEES+    ++   DNK   K  +E D     IE +      G +  + KSQPIP   N  V++VV ++ DD+V  S K+VL+E Y PWC  C+  +  ++++A  F+   +++IAK+DA+AN+ P     V+ +PT+ F  A    N +      + ++   F+++  ++K     ++S K E+ K+ ++ A KDEL
Sbjct:  302 VGDAESSQGAFQYFGLEESQVPLIIIQTPDNKKYLKVNVEVD----QIESWFKDFQDGKVAVHKKSQPIPAENNEPVKVVVAESLDDIVFKSGKNVLIEFYAPWCGHCQKLAPILDEVALSFQNDPSVIIAKLDATANDIPSDTFDVKGFPTIYFRSA--SGNVVVYEGDRTKEDFINFVEKNSEKKPTSHGEESTKSEEPKKTEETAAKDEL 508          
The following BLAST results are available for this feature:
BLAST of CF835073 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 51
Match NameE-valueIdentityDescription
PDIA1_CRIGR2.552e-2330.13Protein disulfide-isomerase OS=Cricetulus griseus ... [more]
PDIA1_MOUSE4.354e-2329.46Protein disulfide-isomerase OS=Mus musculus GN=P4h... [more]
PDI_HUMIN9.699e-2333.33Protein disulfide-isomerase OS=Humicola insolens P... [more]
PDI_ASPNG9.699e-2337.67Protein disulfide-isomerase OS=Aspergillus niger G... [more]
PDIA1_RAT9.699e-2329.69Protein disulfide-isomerase OS=Rattus norvegicus G... [more]
PDI_ASPOR1.267e-2233.13Protein disulfide-isomerase OS=Aspergillus oryzae ... [more]
PDI_MEDSA3.686e-2232.56Protein disulfide-isomerase OS=Medicago sativa GN=... [more]
PDI_DATGL1.072e-2129.56Protein disulfide-isomerase OS=Datisca glomerata G... [more]
PDIA4_CAEEL1.072e-2133.98Probable protein disulfide-isomerase A4 OS=Caenorh... [more]
PDI2_ARATH1.401e-2130.99Protein disulfide-isomerase 2 OS=Arabidopsis thali... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_06J24_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa06J24, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF835073 ID=CF835073; Name=CF835073; organism=Citrus sinensis; type=EST; length=706bp
TTTTTTTTTTTTTTTCCTTTTTCTGTTAAAGTTTCAATATTCCACCCCTT
TTATAAGATCTATAATTCATCTTTGGGAGCTTGATCCCTTTCTTTCAATT
GTTCATCTTTTGGGGATTGATCTTTTTCTTTCAATTGTTCCTTGATAAAT
GCTGCTATGTTCTTTGAGCTGGACCTTGCTGAAACTTTGATCGGATTTGC
TTTATCACCAGCTGGGTAGAATAAAAGTGTTGGGTACTCTTCAACCTGCA
GTTTTGGATGCTCATTTGCAGAAGCATCTATCTTTGCAATGACTAGATTG
TCCAAACCCTTGAAGTGCTTAGCCAGCTTTTCGATTTGCTTGCTAGTTGT
CTCACATCTGACACACCATGGTGTATATACCTCTAGAAGAACATCCTTGT
GACTGTTCAAAACCAAGTCGTCAAATGTCTTTCCCACAACAATCTGCACG
TTTGCGTTTGTGTTATCTGGTATTGGCTGTGATTTCAAGTAAGGCGTCAG
AGTACCATGCAAAAGCCTTGAGCAAAACTCTTCTATGTTGCTTGGCGTGA
GATCTGACTCCAACAAAAACTTTGAAATGGCTTTGTTATCAAAGGCAGTC
ACCACAGTGTTCTTTGATTCTTCAAGCCCAAATAAAGTTAAAAATGGCTT
CGCCAGGTCTTCATCTGCGATATCTACTGCTGTAAACATGAATCTTTCCC
TTGAAA
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