CF835163

Overview
NameCF835163
Unique NameCF835163
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length657
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK_ACACA (S-adenosylmethionine synthetase OS=Acanthamoeba castellanii GN=metK PE=2 SV=1)

HSP 1 Score: 144.05 bits (362), Expect = 6.299e-34
Identity = 82/142 (57.75%), Postives = 94/142 (66.20%), Query Frame = -3 
Query:  236 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGT--GKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKW 655
            LTG KIIIDTY            GKDP+KVDRS AY  R  AKS+VA GLA R +VQVSYAIGV  PLSVFVDSYGT  G   D+++L I K NFD RPG I  +L L+R     + KTA +GHFGR+DPDF WE  K L +
Sbjct:  250 LTGGKIIIDTYGGWGAHGGGAFSGKDPSKVDRSAAYAARWIAKSLVARGLANRALVQVSYAIGVSHPLSVFVDSYGTAQGGRTDEDLLAISKSNFDLRPGKIINDLQLRR---PIYEKTAYHGHFGRNDPDFLWEAPKKLNF 388
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK_LEPCP (S-adenosylmethionine synthetase OS=Leptothrix cholodnii (strain ATCC 51168 / LMG 8142 / SP-6) GN=metK PE=3 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 8.226e-34
Identity = 76/133 (57.14%), Postives = 93/133 (69.92%), Query Frame = -3 
Query:  257 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 655
            LTGRKII+DTY            GKDPTKVDRS AY  R  AK+IVA+GLAR+C +QV+YAIGV  P+++ V + GTG IPD EI K+V+ +FD RP  I   LDL R     + KTAAYGHFGR++P+FTWE
Sbjct:  250 LTGRKIIVDTYGGACPHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAGLARQCQIQVAYAIGVARPMNITVYTEGTGVIPDAEIAKLVEAHFDLRPKGIIQMLDLLR---PIYQKTAAYGHFGREEPEFTWE 379
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK_DIAST (S-adenosylmethionine synthetase OS=Diaphorobacter sp. (strain TPSY) GN=metK PE=3 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 8.226e-34
Identity = 75/133 (56.39%), Postives = 94/133 (70.68%), Query Frame = -3 
Query:  257 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 655
            LTGRKII+DTY            GKDPTKVDRS AY  R  AK+IVA+GLAR+C +QV+YAIGV  P+++ V + GTG IPD +I ++V+E+FD RP  I   LDL R     + KTAAYGHFGR++P+FTWE
Sbjct:  250 LTGRKIIVDTYGGACPHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAGLARQCQIQVAYAIGVARPMNITVYTEGTGVIPDDQIAQLVQEHFDLRPKGIIQMLDLLR---PIYAKTAAYGHFGREEPEFTWE 379
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK_BURTA (S-adenosylmethionine synthetase OS=Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301) GN=metK PE=3 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 8.226e-34
Identity = 75/133 (56.39%), Postives = 95/133 (71.43%), Query Frame = -3 
Query:  257 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 655
            LTGRKII+DTY            GKDP+KVDRS AY  R  AK+IVA+GLA R ++QVSYAIGV EP SV V+++GTG++ D+ I K+V+E+FD RP  I   LDL R     + KTAAYGHFGR++P+F+WE
Sbjct:  245 LTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAEPTSVMVNTFGTGRVSDETITKLVREHFDLRPKGIIQMLDLLR---PIYEKTAAYGHFGREEPEFSWE 374
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK_BURPS (S-adenosylmethionine synthetase OS=Burkholderia pseudomallei GN=metK PE=1 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 8.226e-34
Identity = 75/133 (56.39%), Postives = 95/133 (71.43%), Query Frame = -3 
Query:  257 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 655
            LTGRKII+DTY            GKDP+KVDRS AY  R  AK+IVA+GLA R ++QVSYAIGV EP SV V+++GTG++ D+ I K+V+E+FD RP  I   LDL R     + KTAAYGHFGR++P+F+WE
Sbjct:  245 LTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAEPTSVMVNTFGTGRVSDETITKLVREHFDLRPKGIIQMLDLLR---PIYEKTAAYGHFGREEPEFSWE 374
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK_BURPP (S-adenosylmethionine synthetase OS=Burkholderia phytofirmans (strain DSM 17436 / PsJN) GN=metK PE=3 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 8.226e-34
Identity = 74/133 (55.64%), Postives = 95/133 (71.43%), Query Frame = -3 
Query:  257 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 655
            LTGRKII+DTY            GKDP+KVDRS AY  R  AK+IVA+GLA RC++QVSYAIGV +P SV V+++GTG++ D  I ++V+E+FD RP  I   LDL R     + K+AAYGHFGR++P+FTWE
Sbjct:  245 LTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRCLIQVSYAIGVAQPTSVMVNTFGTGRVSDATITRLVQEHFDLRPKGIIQMLDLLR---PIYEKSAAYGHFGREEPEFTWE 374
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK_BURP6 (S-adenosylmethionine synthetase OS=Burkholderia pseudomallei (strain 668) GN=metK PE=3 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 8.226e-34
Identity = 75/133 (56.39%), Postives = 95/133 (71.43%), Query Frame = -3 
Query:  257 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 655
            LTGRKII+DTY            GKDP+KVDRS AY  R  AK+IVA+GLA R ++QVSYAIGV EP SV V+++GTG++ D+ I K+V+E+FD RP  I   LDL R     + KTAAYGHFGR++P+F+WE
Sbjct:  245 LTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAEPTSVMVNTFGTGRVSDETITKLVREHFDLRPKGIIQMLDLLR---PIYEKTAAYGHFGREEPEFSWE 374
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK_BURP1 (S-adenosylmethionine synthetase OS=Burkholderia pseudomallei (strain 1710b) GN=metK PE=3 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 8.226e-34
Identity = 75/133 (56.39%), Postives = 95/133 (71.43%), Query Frame = -3 
Query:  257 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 655
            LTGRKII+DTY            GKDP+KVDRS AY  R  AK+IVA+GLA R ++QVSYAIGV EP SV V+++GTG++ D+ I K+V+E+FD RP  I   LDL R     + KTAAYGHFGR++P+F+WE
Sbjct:  245 LTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAEPTSVMVNTFGTGRVSDETITKLVREHFDLRPKGIIQMLDLLR---PIYEKTAAYGHFGREEPEFSWE 374
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK_BURP0 (S-adenosylmethionine synthetase OS=Burkholderia pseudomallei (strain 1106a) GN=metK PE=3 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 8.226e-34
Identity = 75/133 (56.39%), Postives = 95/133 (71.43%), Query Frame = -3 
Query:  257 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 655
            LTGRKII+DTY            GKDP+KVDRS AY  R  AK+IVA+GLA R ++QVSYAIGV EP SV V+++GTG++ D+ I K+V+E+FD RP  I   LDL R     + KTAAYGHFGR++P+F+WE
Sbjct:  245 LTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAEPTSVMVNTFGTGRVSDETITKLVREHFDLRPKGIIQMLDLLR---PIYEKTAAYGHFGREEPEFSWE 374
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK_BURMS (S-adenosylmethionine synthetase OS=Burkholderia mallei (strain SAVP1) GN=metK PE=3 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 8.226e-34
Identity = 75/133 (56.39%), Postives = 95/133 (71.43%), Query Frame = -3 
Query:  257 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 655
            LTGRKII+DTY            GKDP+KVDRS AY  R  AK+IVA+GLA R ++QVSYAIGV EP SV V+++GTG++ D+ I K+V+E+FD RP  I   LDL R     + KTAAYGHFGR++P+F+WE
Sbjct:  245 LTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAEPTSVMVNTFGTGRVSDETITKLVREHFDLRPKGIIQMLDLLR---PIYEKTAAYGHFGREEPEFSWE 374
The following BLAST results are available for this feature:
BLAST of CF835163 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK_ACACA6.299e-3457.75S-adenosylmethionine synthetase OS=Acanthamoeba ca... [more]
METK_LEPCP8.226e-3457.14S-adenosylmethionine synthetase OS=Leptothrix chol... [more]
METK_DIAST8.226e-3456.39S-adenosylmethionine synthetase OS=Diaphorobacter ... [more]
METK_BURTA8.226e-3456.39S-adenosylmethionine synthetase OS=Burkholderia th... [more]
METK_BURPS8.226e-3456.39S-adenosylmethionine synthetase OS=Burkholderia ps... [more]
METK_BURPP8.226e-3455.64S-adenosylmethionine synthetase OS=Burkholderia ph... [more]
METK_BURP68.226e-3456.39S-adenosylmethionine synthetase OS=Burkholderia ps... [more]
METK_BURP18.226e-3456.39S-adenosylmethionine synthetase OS=Burkholderia ps... [more]
METK_BURP08.226e-3456.39S-adenosylmethionine synthetase OS=Burkholderia ps... [more]
METK_BURMS8.226e-3456.39S-adenosylmethionine synthetase OS=Burkholderia ma... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_06M13_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa06M13, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF835163 ID=CF835163; Name=CF835163; organism=Citrus sinensis; type=EST; length=657bp
TTGGTTTACCCTTAAGAGAATTTCAGAACAATTCATATTCGCCTCTCCCC
CCATTATGCCTTGGATTGAGAATCCATTAAACATATTTATTAAAAAAAAA
GGGGAAAAGAAGAAGAAAGAAAGAAAGAAAACATTCATGTAACTGAAACA
GAAGTAAAAAAGAAAAGTAGACATATATAAGCACCAATGAGAGGCCAGCA
GCTCATCCTTGATTAGTTTAAGCTTGAAGTTTCTCCCACTTGAGTGGCTT
AACAATCTCCCAAGTGAAATCAGGATCATCTCTGCCAAAGTGCCCATAAG
CGGCAGTCTTCAAGAACCTGCCGTTGCCACCCCTCTTGAGATCAAGATTA
ATAGAAATCATCCCAGGCCTAAAATCAAAATTCTCCTTCACAATCTTCAA
AATTTCCTTGTCAGGGATCTTCCCAGTCCCATAACTATCAACAAAAACTG
ACAAAGGCTCGGGCACACCAATAGCGTAAGAAACCTGAACAATGCATCTC
CTGGCAAGCCCGCTTGCCACAATACTCTTAGCAGCTTGCCTTACAATGTA
AGCACCGCTTCTATCAACCTTGGTAGGATCTTTCCCTGAGAAAGCCCCAC
CACCGTGTGCCCCCCAGCCACCATAAGTATCAATAATAATCTTCCGGCCG
GTGAGCC
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