CF835163

Overview
NameCF835163
Unique NameCF835163
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length657
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CF835163_ssr113 microsatellite CF835163_ssr113:113..128. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig10947 contig Csv1_Contig10947:93..750. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK_PINBN (S-adenosylmethionine synthetase OS=Pinus banksiana GN=METK PE=2 SV=1)

HSP 1 Score: 245.358 bits (625), Expect = 2.007e-64
Identity = 122/145 (84.14%), Postives = 128/145 (88.28%), Query Frame = -3 
Query:  221 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 655
            LTGRKIIIDTY            GKDPTKVDRSGAYIVRQAAKSIVA+GLARRCIVQVSYAIGVPEPLS+FVD+YGTG IPDKEIL+I+KE+FDFRPGMISINLDLKRGGNGR  KTAAYGHFGRDDPDFTWE VKPLKWEK QA
Sbjct:  249 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSLFVDTYGTGSIPDKEILEIIKEHFDFRPGMISINLDLKRGGNGRSQKTAAYGHFGRDDPDFTWETVKPLKWEKAQA 393
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK2_POPTR (S-adenosylmethionine synthetase 2 OS=Populus trichocarpa GN=METK2 PE=2 SV=1)

HSP 1 Score: 245.358 bits (625), Expect = 2.007e-64
Identity = 124/144 (86.11%), Postives = 128/144 (88.89%), Query Frame = -3 
Query:  224 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQ 655
            LTGRKIIIDTY            GKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVD+YGTGKIPDKEIL IVKE FDFRPGMI+I+LDLKRGGNGRFLKTAAYGHFGRDDPDFTWE+VKPLK EK Q
Sbjct:  249 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILNIVKEKFDFRPGMIAISLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKSEKPQ 392
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK2_DAUCA (S-adenosylmethionine synthetase 2 OS=Daucus carota GN=SAMS2 PE=2 SV=1)

HSP 1 Score: 245.358 bits (625), Expect = 2.007e-64
Identity = 123/145 (84.83%), Postives = 127/145 (87.59%), Query Frame = -3 
Query:  221 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 655
            LTGR IIIDTY            GKDPTKVDRSGAYIVRQAAKSIVA+GLARRCIVQVSYAIGVP+PLSVFVDSYGTG IPDKEILKIVKE FDFRPGMI+INLDLKRGGN RFLKTAAYGHFGRDD DFTWE+VKPLKWEK QA
Sbjct:  249 LTGRMIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPDPLSVFVDSYGTGNIPDKEILKIVKETFDFRPGMIAINLDLKRGGNNRFLKTAAYGHFGRDDADFTWEVVKPLKWEKPQA 393
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK_BRARP (S-adenosylmethionine synthetase OS=Brassica rapa subsp. pekinensis GN=SAMS PE=2 SV=1)

HSP 1 Score: 244.973 bits (624), Expect = 2.622e-64
Identity = 120/145 (82.76%), Postives = 130/145 (89.66%), Query Frame = -3 
Query:  221 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 655
            LTGRKIIIDTY            GKDPTKVDRSGAYIVRQAAKS+VA+G+ARR +VQVSYAIGVPEPLSVFVD+YGTG IPDKEILKIVKE+FDFRPGM++INLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE+VKPLKW+K QA
Sbjct:  249 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSVVANGMARRALVQVSYAIGVPEPLSVFVDTYGTGLIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWDKPQA 393
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK5_BRAJU (S-adenosylmethionine synthetase 5 OS=Brassica juncea GN=MSAMS5 PE=2 SV=1)

HSP 1 Score: 244.973 bits (624), Expect = 2.622e-64
Identity = 120/145 (82.76%), Postives = 130/145 (89.66%), Query Frame = -3 
Query:  221 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 655
            LTGRKIIIDTY            GKDPTKVDRSGAYIVRQAAKS+VA+G+ARR +VQVSYAIGVPEPLSVFVD+YGTG IPDKEILKIVKE+FDFRPGM++INLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE+VKPLKW+K QA
Sbjct:  249 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSVVANGMARRALVQVSYAIGVPEPLSVFVDTYGTGLIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWDKPQA 393
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK3_BRAJU (S-adenosylmethionine synthetase 3 OS=Brassica juncea GN=MSAMS3 PE=2 SV=1)

HSP 1 Score: 244.973 bits (624), Expect = 2.622e-64
Identity = 120/145 (82.76%), Postives = 130/145 (89.66%), Query Frame = -3 
Query:  221 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 655
            LTGRKIIIDTY            GKDPTKVDRSGAYIVRQAAKS+VA+G+ARR +VQVSYAIGVPEPLSVFVD+YGTG IPDKEILKIVKE+FDFRPGM++INLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE+VKPLKW+K QA
Sbjct:  249 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSVVANGMARRALVQVSYAIGVPEPLSVFVDTYGTGLIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWDKPQA 393
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK4_ARATH (S-adenosylmethionine synthetase 4 OS=Arabidopsis thaliana GN=METK4 PE=1 SV=1)

HSP 1 Score: 244.588 bits (623), Expect = 3.424e-64
Identity = 125/145 (86.21%), Postives = 129/145 (88.97%), Query Frame = -3 
Query:  221 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 655
            LTGRKIIIDTY            GKDPTKVDRSGAYIVRQAAKSIVASGLARR IVQVSYAIGVPEPLSVFVDSYGTGKIPDKEIL+IVKE+FDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDD DFTWE+VKPLK  K+QA
Sbjct:  249 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGLARRVIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILEIVKESFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDADFTWEVVKPLKSNKVQA 393
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK4_HORVU (S-adenosylmethionine synthetase 4 OS=Hordeum vulgare GN=SAM4 PE=2 SV=1)

HSP 1 Score: 242.662 bits (618), Expect = 1.301e-63
Identity = 121/145 (83.45%), Postives = 127/145 (87.59%), Query Frame = -3 
Query:  221 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 655
            LTGRKIIIDTY            GKDPTKVDRSGAYI RQAAKSIVASG+ARRCIVQVSYAIGVPEPLSVFVD+YGTGKIPDKEIL+IVKENFDFRPGMI INLDLKRGG+GR+LKTAAYGHFGRD  DFTWE+VKPLKWEK  A
Sbjct:  252 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIARQAAKSIVASGIARRCIVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILEIVKENFDFRPGMIIINLDLKRGGSGRYLKTAAYGHFGRDGADFTWEVVKPLKWEKPSA 396
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK2_ARATH (S-adenosylmethionine synthetase 2 OS=Arabidopsis thaliana GN=SAM2 PE=1 SV=1)

HSP 1 Score: 242.276 bits (617), Expect = 1.699e-63
Identity = 119/145 (82.07%), Postives = 128/145 (88.28%), Query Frame = -3 
Query:  221 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 655
            LTGRKIIIDTY            GKDPTKVDRSGAYIVRQAAKS+VA+G+ARR +VQVSYAIGVPEPLSVFVD+YGTG IPDKEILKIVKE FDFRPGM++INLDLKRGGNGRF KTAAYGHFGRDDPDFTWE+VKPLKW+K QA
Sbjct:  249 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSVVANGMARRALVQVSYAIGVPEPLSVFVDTYGTGLIPDKEILKIVKETFDFRPGMMTINLDLKRGGNGRFQKTAAYGHFGRDDPDFTWEVVKPLKWDKPQA 393
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK1_BRAJU (S-adenosylmethionine synthetase 1 OS=Brassica juncea GN=SAMS1 PE=2 SV=1)

HSP 1 Score: 242.276 bits (617), Expect = 1.699e-63
Identity = 118/145 (81.38%), Postives = 129/145 (88.97%), Query Frame = -3 
Query:  221 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 655
            LTGRKIIIDTY            GKDPTKVDRSGAYIVRQ+AKS+VA+G+ARR +VQVSYAIGVPEP SVFVD+YGTG IPDKEILKIVKE+FDFRPGM++INLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE+VKPLKW+K QA
Sbjct:  249 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQSAKSVVANGMARRALVQVSYAIGVPEPFSVFVDTYGTGLIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWDKPQA 393
The following BLAST results are available for this feature:
BLAST of CF835163 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK_PINBN2.007e-6484.14S-adenosylmethionine synthetase OS=Pinus banksiana... [more]
METK2_POPTR2.007e-6486.11S-adenosylmethionine synthetase 2 OS=Populus trich... [more]
METK2_DAUCA2.007e-6484.83S-adenosylmethionine synthetase 2 OS=Daucus carota... [more]
METK_BRARP2.622e-6482.76S-adenosylmethionine synthetase OS=Brassica rapa s... [more]
METK5_BRAJU2.622e-6482.76S-adenosylmethionine synthetase 5 OS=Brassica junc... [more]
METK3_BRAJU2.622e-6482.76S-adenosylmethionine synthetase 3 OS=Brassica junc... [more]
METK4_ARATH3.424e-6486.21S-adenosylmethionine synthetase 4 OS=Arabidopsis t... [more]
METK4_HORVU1.301e-6383.45S-adenosylmethionine synthetase 4 OS=Hordeum vulga... [more]
METK2_ARATH1.699e-6382.07S-adenosylmethionine synthetase 2 OS=Arabidopsis t... [more]
METK1_BRAJU1.699e-6381.38S-adenosylmethionine synthetase 1 OS=Brassica junc... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_06M13_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa06M13, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF835163 ID=CF835163; Name=CF835163; organism=Citrus sinensis; type=EST; length=657bp
TTGGTTTACCCTTAAGAGAATTTCAGAACAATTCATATTCGCCTCTCCCC
CCATTATGCCTTGGATTGAGAATCCATTAAACATATTTATTAAAAAAAAA
GGGGAAAAGAAGAAGAAAGAAAGAAAGAAAACATTCATGTAACTGAAACA
GAAGTAAAAAAGAAAAGTAGACATATATAAGCACCAATGAGAGGCCAGCA
GCTCATCCTTGATTAGTTTAAGCTTGAAGTTTCTCCCACTTGAGTGGCTT
AACAATCTCCCAAGTGAAATCAGGATCATCTCTGCCAAAGTGCCCATAAG
CGGCAGTCTTCAAGAACCTGCCGTTGCCACCCCTCTTGAGATCAAGATTA
ATAGAAATCATCCCAGGCCTAAAATCAAAATTCTCCTTCACAATCTTCAA
AATTTCCTTGTCAGGGATCTTCCCAGTCCCATAACTATCAACAAAAACTG
ACAAAGGCTCGGGCACACCAATAGCGTAAGAAACCTGAACAATGCATCTC
CTGGCAAGCCCGCTTGCCACAATACTCTTAGCAGCTTGCCTTACAATGTA
AGCACCGCTTCTATCAACCTTGGTAGGATCTTTCCCTGAGAAAGCCCCAC
CACCGTGTGCCCCCCAGCCACCATAAGTATCAATAATAATCTTCCGGCCG
GTGAGCC
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