CF835164

Overview
NameCF835164
Unique NameCF835164
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length548
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CF835164_ssr33 microsatellite CF835164_ssr33:33..42. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig12594 contig Csv1_Contig12594:11..558. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK_MUSAC (S-adenosylmethionine synthetase OS=Musa acuminata GN=METK PE=2 SV=1)

HSP 1 Score: 231.491 bits (589), Expect = 2.055e-60
Identity = 109/118 (92.37%), Postives = 112/118 (94.92%), Query Frame = 1 
Query:  193 ETFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            +TFLFTSESVNEGHPDKLCDQ+SDAVLDACL QDPDSKVACETCTKTNM MVFGEITTK N+DYEKIVRDTCR IGFTS DVGLDADHCKVLVNIEQQSPDIAQGVHGH TKRPEEIG
Sbjct:    3 DTFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKGNIDYEKIVRDTCRDIGFTSEDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIG 120
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK2_SOLLC (S-adenosylmethionine synthetase 2 OS=Solanum lycopersicum GN=SAM2 PE=2 SV=1)

HSP 1 Score: 231.491 bits (589), Expect = 2.055e-60
Identity = 110/119 (92.44%), Postives = 115/119 (96.64%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDP+SKVACETCTKTN+ MVFGEITTKAN+DYEKIVRDTCR IGF S DVGLDAD+C+VLVNIEQQSPDIAQGVHGHLTKRPEEIG
Sbjct:    1 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPESKVACETCTKTNLVMVFGEITTKANIDYEKIVRDTCREIGFVSPDVGLDADNCRVLVNIEQQSPDIAQGVHGHLTKRPEEIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK2_HORVU (S-adenosylmethionine synthetase 2 OS=Hordeum vulgare GN=SAM2 PE=2 SV=1)

HSP 1 Score: 231.491 bits (589), Expect = 2.055e-60
Identity = 111/118 (94.07%), Postives = 112/118 (94.92%), Query Frame = 1 
Query:  193 ETFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            ETFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNM MVFGEITTKA VDYEKIVRDTCR IGF S DVGLDADHCKVLVNIEQQSPDIAQGVHGH TKRPEE+G
Sbjct:    4 ETFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRDIGFISDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEVG 121
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK2_CATRO (S-adenosylmethionine synthetase 2 OS=Catharanthus roseus GN=SAMS2 PE=1 SV=1)

HSP 1 Score: 231.491 bits (589), Expect = 2.055e-60
Identity = 111/119 (93.28%), Postives = 115/119 (96.64%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            METFLFTSESVNEGHPDKLCDQ+SDAVLDACLAQDP+SKVACETCTKTNM MVFGEITTKA VDYEKIVRDTCR+IGF S DVGLDAD+CKVLVNIEQQSPDIAQGVHGHLTKRPEEIG
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLAQDPESKVACETCTKTNMVMVFGEITTKAQVDYEKIVRDTCRAIGFVSDDVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK1_HORVU (S-adenosylmethionine synthetase 1 OS=Hordeum vulgare GN=SAM1 PE=2 SV=1)

HSP 1 Score: 231.491 bits (589), Expect = 2.055e-60
Identity = 111/118 (94.07%), Postives = 112/118 (94.92%), Query Frame = 1 
Query:  193 ETFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            ETFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNM MVFGEITTKA VDYEKIVRDTCR IGF S DVGLDADHCKVLVNIEQQSPDIAQGVHGH TKRPEE+G
Sbjct:    4 ETFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRDIGFISDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEVG 121
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK3_SOLLC (S-adenosylmethionine synthetase 3 OS=Solanum lycopersicum GN=SAM3 PE=2 SV=1)

HSP 1 Score: 230.335 bits (586), Expect = 4.577e-60
Identity = 110/119 (92.44%), Postives = 114/119 (95.80%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            METFLFTSESVNEGHPDKLCDQVSDA+LDACL QDP+SKVACETCTKTNM MVFGEITTKA VDYEKIVRDTCR IGF SADVGLDAD+CKVLVNIEQQSPDIAQGVHGHLTK+PEEIG
Sbjct:    1 METFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRGIGFVSADVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKKPEEIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK1_PICSI (S-adenosylmethionine synthetase 1 OS=Picea sitchensis GN=METK1 PE=2 SV=1)

HSP 1 Score: 230.335 bits (586), Expect = 4.577e-60
Identity = 107/119 (89.92%), Postives = 115/119 (96.64%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            M+TFLFTSESVNEGHPDKLCDQ+SDA+LDACL QDP+SKVACETCTKTNM M+FGEITTKAN+DYEKIVRDTCR IGF SADVGLDAD+CKVLVNIEQQSPDIAQGVHGHLTK+PEEIG
Sbjct:    1 MDTFLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNMVMIFGEITTKANIDYEKIVRDTCRGIGFVSADVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKKPEEIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK2_SOLTU (S-adenosylmethionine synthetase 2 OS=Solanum tuberosum GN=METK2 PE=2 SV=1)

HSP 1 Score: 229.95 bits (585), Expect = 5.978e-60
Identity = 110/119 (92.44%), Postives = 114/119 (95.80%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            METFL TSESVNEGHPDKLCDQVSDA+LDACL QDP+SKVACETCTKTNM MVFGEITTKANVDYEKIVRDTCR IGF SADVGLDAD+CKVLVNIEQQSPDIAQGVHGHLTK+PEEIG
Sbjct:    1 METFLLTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCREIGFISADVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKKPEEIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK_PINBN (S-adenosylmethionine synthetase OS=Pinus banksiana GN=METK PE=2 SV=1)

HSP 1 Score: 229.565 bits (584), Expect = 7.807e-60
Identity = 110/119 (92.44%), Postives = 113/119 (94.96%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            METFLFTSESVNEGHPDKLCDQ+SDAVLDACLAQDPDSKVACETCTKTNM MVFGEITTKA+VDYE+IVR TCR IGF S DVGLDADHCKVLVNIEQQSPDIAQGVHGH TKRPEEIG
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKADVDYEQIVRKTCREIGFISDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK5_POPTR (S-adenosylmethionine synthetase 5 OS=Populus trichocarpa GN=METK5 PE=2 SV=1)

HSP 1 Score: 229.565 bits (584), Expect = 7.807e-60
Identity = 110/118 (93.22%), Postives = 115/118 (97.46%), Query Frame = 1 
Query:  193 ETFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            ETFLFTSESVNEGHPDKLCDQ+SDAVLDACLAQDPDSKVACETCTKTNM MVFGEITTKA+VDYEKIVRDTCR+IGFTSADVGLDAD+CKVLVNIEQQS DIAQGVHGH +KRPEEIG
Sbjct:    3 ETFLFTSESVNEGHPDKLCDQISDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKADVDYEKIVRDTCRNIGFTSADVGLDADNCKVLVNIEQQSSDIAQGVHGHFSKRPEEIG 120
The following BLAST results are available for this feature:
BLAST of CF835164 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK_MUSAC2.055e-6092.37S-adenosylmethionine synthetase OS=Musa acuminata ... [more]
METK2_SOLLC2.055e-6092.44S-adenosylmethionine synthetase 2 OS=Solanum lycop... [more]
METK2_HORVU2.055e-6094.07S-adenosylmethionine synthetase 2 OS=Hordeum vulga... [more]
METK2_CATRO2.055e-6093.28S-adenosylmethionine synthetase 2 OS=Catharanthus ... [more]
METK1_HORVU2.055e-6094.07S-adenosylmethionine synthetase 1 OS=Hordeum vulga... [more]
METK3_SOLLC4.577e-6092.44S-adenosylmethionine synthetase 3 OS=Solanum lycop... [more]
METK1_PICSI4.577e-6089.92S-adenosylmethionine synthetase 1 OS=Picea sitchen... [more]
METK2_SOLTU5.978e-6092.44S-adenosylmethionine synthetase 2 OS=Solanum tuber... [more]
METK_PINBN7.807e-6092.44S-adenosylmethionine synthetase OS=Pinus banksiana... [more]
METK5_POPTR7.807e-6093.22S-adenosylmethionine synthetase 5 OS=Populus trich... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_06M13_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa06M13, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF835164 ID=CF835164; Name=CF835164; organism=Citrus sinensis; type=EST; length=548bp
GAAACTACAAATCCTTGCTGCCGTTTTACCTCCTCTCTCTCTTCTCTCAG
CGTATGCATATCGGCTTTCTCTCGTTCTTCTGGGTACTTACCATTTTCGT
ATATCACCACCTTCAATTCCATTTGTACTCGCACCACGGCCTCCTGCAAA
GCGAAAGCTGCAGCTTCAGGTTAAATCAAATTAAAGAAAATGGAAACATT
TCTTTTCACATCAGAATCAGTGAACGAGGGTCACCCGGACAAGCTGTGTG
ACCAAGTTTCGGACGCAGTACTGGACGCGTGTTTGGCTCAGGATCCTGAC
AGCAAAGTTGCTTGTGAAACATGCACAAAGACCAACATGGACATGGTTTT
TGGAGAAATTACAACCAAGGCCAATGTGGATTACGAGAAAATCGTGCGTG
ATACATGCAGATCGATTGGGTTCACATCGGCCGATGTGGGGCTTGATGCT
GATCACTGCAAAGTTCTTGTTAATATTGAGCAGCAAAGCCCTGACATTGC
TCAGGGTGTTCATGGGCATCTCACAAAGCGGCCTGAGGAGATTGGTGC
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