CK739740 | Citrus Genome Database

Overview


Name	CK739740
Unique Name	CK739740
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	539

Libraries


Library Name	Type
Ridge pineapple sweet orange entire seedling	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig1582	contig	Csv1_Contig1582:264..806.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CK739740 vs. ExPASy Swiss-Prot
Match: 1433E_BOVIN (14-3-3 protein epsilon OS=Bos taurus GN=YWHAE PE=2 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 9.551e-49
Identity = 74/134 (55.22%), Postives = 88/134 (65.67%), Query Frame = 1

Query:    1 RASWRILSSIEQKEEAKG----MNKCEED*GI*AEG*R*ACKDL**YIVCH**TPNPSSTSGESTVFYYKMKGDYYRYLAEFKGADDRKEAADHSLKXXXXXXXXXXSELPPTHPIRLGLALNFSVFYYEILNS 390
            RASWRI+SSIEQKEE KG    +    E   +     +  C D+   +  H     P++ +GES VFYYKMKGDY+RYLAEF   +DRKEAA++SL           +ELPPTHPIRLGLALNFSVFYYEILNS
Sbjct:   57 RASWRIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKH---LIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNS 187

HSP 2 Score: 87.4261 bits (215), Expect = 9.551e-49
Identity = 41/48 (85.42%), Postives = 46/48 (95.83%), Query Frame = 3

Query:  390 PERACHLAKQAFDEAIAELDSLNEESYKDSTLIMQLLRDNLTLWTSDL 533
            P+RAC LAK AFD+AIAELD+L+EESYKDSTLIMQLLRDNLTLWTSD+
Sbjct:  188 PDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDM 235

[Close]

BLAST of CK739740 vs. ExPASy Swiss-Prot
Match: 14331_ARATH (14-3-3-like protein GF14 chi OS=Arabidopsis thaliana GN=GRF1 PE=1 SV=3)

HSP 1 Score: 122.094 bits (305), Expect = 1.244e-48
Identity = 73/141 (51.77%), Postives = 88/141 (62.41%), Query Frame = 1

Query:    1 RASWRILSSIEQKEEAKG-----------MNKCEED*GI*AEG*R*ACKDL**YIVCH**TPNPSSTSGESTVFYYKMKGDYYRYLAEFKGADDRKEAADHSLKXXXXXXXXXXSELPPTHPIRLGLALNFSVFYYEILNS 390
            RASWRI+SSIEQKEE++G            +K E +     +G     K L   +V       P++ SG+S VFY KMKGDY+RYLAEFK   +RK+AA+H+L           SEL PTHPIRLGLALNFSVFYYEILNS
Sbjct:   65 RASWRIISSIEQKEESRGNDDHVSLIRDYRSKIETELSDICDG---ILKLLDTILV-------PAAASGDSKVFYLKMKGDYHRYLAEFKSGQERKDAAEHTLTAYKAAQDIANSELAPTHPIRLGLALNFSVFYYEILNS 195

HSP 2 Score: 91.2781 bits (225), Expect = 1.244e-48
Identity = 43/50 (86.00%), Postives = 48/50 (96.00%), Query Frame = 3

Query:  390 PERACHLAKQAFDEAIAELDSLNEESYKDSTLIMQLLRDNLTLWTSDLPE 539
            P+RAC+LAKQAFDEAIAELD+L EESYKDSTLIMQLLRDNLTLWTSD+ +
Sbjct:  196 PDRACNLAKQAFDEAIAELDTLGEESYKDSTLIMQLLRDNLTLWTSDMQD 245

[Close]

BLAST of CK739740 vs. ExPASy Swiss-Prot
Match: 14332_ORYSJ (14-3-3-like protein GF14-B OS=Oryza sativa subsp. japonica GN=GF14B PE=1 SV=2)

HSP 1 Score: 121.709 bits (304), Expect = 1.244e-48
Identity = 74/141 (52.48%), Postives = 89/141 (63.12%), Query Frame = 1

Query:    1 RASWRILSSIEQKEEAKG-----------MNKCEED*GI*AEG*R*ACKDL**YIVCH**TPNPSSTSGESTVFYYKMKGDYYRYLAEFKGADDRKEAADHSLKXXXXXXXXXXSELPPTHPIRLGLALNFSVFYYEILNS 390
            RASWRI+SSIEQKEE++G             K E +     +G     K L  ++V       PSST+ ES VFY KMKGDYYRYLAEFK   +RK+AA++++           +ELPPTHPIRLGLALNFSVFYYEILNS
Sbjct:   64 RASWRIISSIEQKEESRGNEDRVTLIKDYRGKIETELTKICDG---ILKLLESHLV-------PSSTAPESKVFYLKMKGDYYRYLAEFKTGAERKDAAENTMVAYKAAQDIALAELPPTHPIRLGLALNFSVFYYEILNS 194

HSP 2 Score: 91.6633 bits (226), Expect = 1.244e-48
Identity = 43/50 (86.00%), Postives = 49/50 (98.00%), Query Frame = 3

Query:  390 PERACHLAKQAFDEAIAELDSLNEESYKDSTLIMQLLRDNLTLWTSDLPE 539
            P+RAC+LAKQAFDEAI+ELD+L+EESYKDSTLIMQLLRDNLTLWTSD+ E
Sbjct:  195 PDRACNLAKQAFDEAISELDTLSEESYKDSTLIMQLLRDNLTLWTSDISE 244

[Close]

BLAST of CK739740 vs. ExPASy Swiss-Prot
Match: 1433_PEA (14-3-3-like protein OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 2.114e-48
Identity = 73/141 (51.77%), Postives = 89/141 (63.12%), Query Frame = 1

Query:    1 RASWRILSSIEQKEEAKG-----------MNKCEED*GI*AEG*R*ACKDL**YIVCH**TPNPSSTSGESTVFYYKMKGDYYRYLAEFKGADDRKEAADHSLKXXXXXXXXXXSELPPTHPIRLGLALNFSVFYYEILNS 390
            RASWRI+SSIEQKEE++G            +K E +     +G     K L   ++       PS++SG+S VFY KMKGDY+RYLAEFK   +RKEAA+ +L           +ELPPTHPIRLGLALNFSVFYYEILNS
Sbjct:   63 RASWRIISSIEQKEESRGNEDHVAVIRDYRSKIESELSNICDG---ILKLLDTRLI-------PSASSGDSKVFYLKMKGDYHRYLAEFKTGAERKEAAESTLTGYKSAQDIANAELPPTHPIRLGLALNFSVFYYEILNS 193

HSP 2 Score: 91.2781 bits (225), Expect = 2.114e-48
Identity = 43/50 (86.00%), Postives = 48/50 (96.00%), Query Frame = 3

Query:  390 PERACHLAKQAFDEAIAELDSLNEESYKDSTLIMQLLRDNLTLWTSDLPE 539
            P+RAC+LAKQAFDEAIAELD+L EESYKDSTLIMQLLRDNLTLWTSD+ +
Sbjct:  194 PDRACNLAKQAFDEAIAELDTLGEESYKDSTLIMQLLRDNLTLWTSDMQD 243

[Close]

BLAST of CK739740 vs. ExPASy Swiss-Prot
Match: RAD24_SCHPO (DNA damage checkpoint protein rad24 OS=Schizosaccharomyces pombe GN=rad24 PE=1 SV=2)

HSP 1 Score: 120.939 bits (302), Expect = 3.590e-48
Identity = 72/141 (51.06%), Postives = 86/141 (60.99%), Query Frame = 1

Query:    1 RASWRILSSIEQKEEAKG-----------MNKCEED*GI*AEG*R*ACKDL**YIVCH**TPNPSSTSGESTVFYYKMKGDYYRYLAEFKGADDRKEAADHSLKXXXXXXXXXXSELPPTHPIRLGLALNFSVFYYEILNS 390
            RASWRI+SSIEQKEE+KG             K E++           C+D+   +  H     P++ S ES VFYYKMKGDYYRYLAEF   + R+ +AD SL+          +EL PTHPIRLGLALNFSVFYYEILNS
Sbjct:   59 RASWRIVSSIEQKEESKGNTAQVELIKEYRQKIEQELDT-------ICQDILTVLEKH---LIPNAASAESKVFYYKMKGDYYRYLAEFAVGEKRQHSADQSLEGYKAASEIATAELAPTHPIRLGLALNFSVFYYEILNS 189

HSP 2 Score: 90.8929 bits (224), Expect = 3.590e-48
Identity = 43/47 (91.49%), Postives = 47/47 (100.00%), Query Frame = 3

Query:  390 PERACHLAKQAFDEAIAELDSLNEESYKDSTLIMQLLRDNLTLWTSD 530
            P+RAC+LAKQAFDEAI+ELDSL+EESYKDSTLIMQLLRDNLTLWTSD
Sbjct:  190 PDRACYLAKQAFDEAISELDSLSEESYKDSTLIMQLLRDNLTLWTSD 236

[Close]

BLAST of CK739740 vs. ExPASy Swiss-Prot
Match: 14334_ARATH (14-3-3-like protein GF14 phi OS=Arabidopsis thaliana GN=GRF4 PE=1 SV=2)

HSP 1 Score: 120.168 bits (300), Expect = 4.680e-48
Identity = 72/141 (51.06%), Postives = 88/141 (62.41%), Query Frame = 1

Query:    1 RASWRILSSIEQKEEAKG-----------MNKCEED*GI*AEG*R*ACKDL**YIVCH**TPNPSSTSGESTVFYYKMKGDYYRYLAEFKGADDRKEAADHSLKXXXXXXXXXXSELPPTHPIRLGLALNFSVFYYEILNS 390
            RASWRI+SSIEQKEE++G            +K E +     +G     K L   +V       P+S +G+S VFY KMKGDY+RYLAEFK   +RK+AA+H+L           +EL PTHPIRLGLALNFSVFYYEILNS
Sbjct:   66 RASWRIISSIEQKEESRGNDDHVTTIRDYRSKIESELSKICDG---ILKLLDTRLV-------PASANGDSKVFYLKMKGDYHRYLAEFKTGQERKDAAEHTLTAYKAAQDIANAELAPTHPIRLGLALNFSVFYYEILNS 196

HSP 2 Score: 91.2781 bits (225), Expect = 4.680e-48
Identity = 43/50 (86.00%), Postives = 48/50 (96.00%), Query Frame = 3

Query:  390 PERACHLAKQAFDEAIAELDSLNEESYKDSTLIMQLLRDNLTLWTSDLPE 539
            P+RAC+LAKQAFDEAIAELD+L EESYKDSTLIMQLLRDNLTLWTSD+ +
Sbjct:  197 PDRACNLAKQAFDEAIAELDTLGEESYKDSTLIMQLLRDNLTLWTSDMQD 246

[Close]

BLAST of CK739740 vs. ExPASy Swiss-Prot
Match: BMH1_YEAST (Protein BMH1 OS=Saccharomyces cerevisiae GN=BMH1 PE=1 SV=4)

HSP 1 Score: 118.242 bits (295), Expect = 4.680e-48
Identity = 72/136 (52.94%), Postives = 87/136 (63.97%), Query Frame = 1

Query:    1 RASWRILSSIEQKEEAKGMNK------CEED*GI*AEG*R*ACKDL**YIVCH**TPNPSSTSGESTVFYYKMKGDYYRYLAEFKGADDRKEAADHSLKXXXXXXXXXXSELPPTHPIRLGLALNFSVFYYEILNS 390
            RASWRI+SSIEQKEE+K  ++      C     I  E  +    D+   +  H     PS+T+GES VFYYKMKGDY+RYLAEF   D R++A + SL+          +ELPPTHPIRLGLALNFSVFYYEI NS
Sbjct:   58 RASWRIVSSIEQKEESKEKSEHQVELICSYRSKIETELTK-ISDDILSVLDSH---LIPSATTGESKVFYYKMKGDYHRYLAEFSSGDAREKATNASLEAYKTASEIATTELPPTHPIRLGLALNFSVFYYEIQNS 189

HSP 2 Score: 93.2041 bits (230), Expect = 4.680e-48
Identity = 43/50 (86.00%), Postives = 49/50 (98.00%), Query Frame = 3

Query:  390 PERACHLAKQAFDEAIAELDSLNEESYKDSTLIMQLLRDNLTLWTSDLPE 539
            P++ACHLAKQAFD+AIAELD+L+EESYKDSTLIMQLLRDNLTLWTSD+ E
Sbjct:  190 PDKACHLAKQAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMSE 239

[Close]

BLAST of CK739740 vs. ExPASy Swiss-Prot
Match: 1433_CANAL (14-3-3 protein homolog OS=Candida albicans GN=BMH1 PE=1 SV=2)

HSP 1 Score: 126.716 bits (317), Expect = 4.680e-48
Identity = 79/135 (58.52%), Postives = 91/135 (67.41%), Query Frame = 1

Query:    1 RASWRILSSIEQKEEAKGMNKC-----EED*GI*AEG*R*ACKDL**YIVCH**TPNPSSTSGESTVFYYKMKGDYYRYLAEFKGADDRKEAADHSLKXXXXXXXXXXSELPPTHPIRLGLALNFSVFYYEILNS 390
            RASWRI+SSIEQKEEAKG         +    I AE  +  C+D+   +  H  T   S+ +GES VFYYKMKGDY+RYLAEF  A+ RKEAAD SL+          +ELPPTHPIRLGLALNFSVFYYEILNS
Sbjct:   58 RASWRIVSSIEQKEEAKGNESQVALIRDYRAKIEAELSK-ICEDILSVLSDHLIT---SAQTGESKVFYYKMKGDYHRYLAEFAIAEKRKEAADLSLEAYKAASDVAVTELPPTHPIRLGLALNFSVFYYEILNS 188

HSP 2 Score: 84.7297 bits (208), Expect = 4.680e-48
Identity = 40/50 (80.00%), Postives = 48/50 (96.00%), Query Frame = 3

Query:  390 PERACHLAKQAFDEAIAELDSLNEESYKDSTLIMQLLRDNLTLWTSDLPE 539
            P+RACHLAKQAFD+A+A+L++L+E+SYKDSTLIMQLLRDNLTLWT DL E
Sbjct:  189 PDRACHLAKQAFDDAVADLETLSEDSYKDSTLIMQLLRDNLTLWT-DLSE 237

[Close]

BLAST of CK739740 vs. ExPASy Swiss-Prot
Match: 14333_SOLLC (14-3-3 protein 3 OS=Solanum lycopersicum GN=TFT3 PE=2 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 6.101e-48
Identity = 73/141 (51.77%), Postives = 88/141 (62.41%), Query Frame = 1

Query:    1 RASWRILSSIEQKEEAKG-----------MNKCEED*GI*AEG*R*ACKDL**YIVCH**TPNPSSTSGESTVFYYKMKGDYYRYLAEFKGADDRKEAADHSLKXXXXXXXXXXSELPPTHPIRLGLALNFSVFYYEILNS 390
            RASWRI+SSIEQKEE++G            +K E +     +G     K L   ++       PS+TSG+S VFY KMKGDY+RYLAEFK   +RKEAA+ +L           +EL PTHPIRLGLALNFSVFYYEILNS
Sbjct:   64 RASWRIISSIEQKEESRGNEEHVNSIREYRSKIENELSKICDG---ILKLLDSKLI-------PSATSGDSKVFYLKMKGDYHRYLAEFKTGAERKEAAESTLTAYKAAQDIASAELAPTHPIRLGLALNFSVFYYEILNS 194

HSP 2 Score: 91.2781 bits (225), Expect = 6.101e-48
Identity = 43/50 (86.00%), Postives = 48/50 (96.00%), Query Frame = 3

Query:  390 PERACHLAKQAFDEAIAELDSLNEESYKDSTLIMQLLRDNLTLWTSDLPE 539
            P+RAC+LAKQAFDEAIAELD+L EESYKDSTLIMQLLRDNLTLWTSD+ +
Sbjct:  195 PDRACNLAKQAFDEAIAELDTLGEESYKDSTLIMQLLRDNLTLWTSDMQD 244

[Close]

BLAST of CK739740 vs. ExPASy Swiss-Prot
Match: 1433_LILLO (14-3-3-like protein OS=Lilium longiflorum PE=2 SV=1)

HSP 1 Score: 119.398 bits (298), Expect = 1.037e-47
Identity = 74/141 (52.48%), Postives = 87/141 (61.70%), Query Frame = 1

Query:    1 RASWRILSSIEQKEEAKG-----------MNKCEED*GI*AEG*R*ACKDL**YIVCH**TPNPSSTSGESTVFYYKMKGDYYRYLAEFKGADDRKEAADHSLKXXXXXXXXXXSELPPTHPIRLGLALNFSVFYYEILNS 390
            RASWRI+SSIEQKEE++G             K E +     +G     K L  ++V       PSST+ ES VFY KMKGDY+RYLAEFK   +RKEAA+ +L           +EL PTHPIRLGLALNFSVFYYEILNS
Sbjct:   63 RASWRIISSIEQKEESRGNEDHVALIKDYRGKIEAELSKICDG---ILKLLDSHLV-------PSSTAPESKVFYLKMKGDYHRYLAEFKSGAERKEAAESTLLAYKSAQDIALAELAPTHPIRLGLALNFSVFYYEILNS 193

HSP 2 Score: 90.8929 bits (224), Expect = 1.037e-47
Identity = 43/50 (86.00%), Postives = 48/50 (96.00%), Query Frame = 3

Query:  390 PERACHLAKQAFDEAIAELDSLNEESYKDSTLIMQLLRDNLTLWTSDLPE 539
            P+RAC+LAKQAFDEAI+ELD+L EESYKDSTLIMQLLRDNLTLWTSD+ E
Sbjct:  194 PDRACNLAKQAFDEAISELDTLGEESYKDSTLIMQLLRDNLTLWTSDINE 243

The following BLAST results are available for this feature:

BLAST of CK739740 vs. ExPASy Swiss-Prot

BLAST of CK739740 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 143


Match Name	E-value	Identity	Description
1433E_BOVIN	9.551e-49	55.22	14-3-3 protein epsilon OS=Bos taurus GN=YWHAE PE=2...	[more]
14331_ARATH	1.244e-48	51.77	14-3-3-like protein GF14 chi OS=Arabidopsis thalia...	[more]
14332_ORYSJ	1.244e-48	52.48	14-3-3-like protein GF14-B OS=Oryza sativa subsp. ...	[more]
1433_PEA	2.114e-48	51.77	14-3-3-like protein OS=Pisum sativum PE=2 SV=1	[more]
RAD24_SCHPO	3.590e-48	51.06	DNA damage checkpoint protein rad24 OS=Schizosacch...	[more]
14334_ARATH	4.680e-48	51.06	14-3-3-like protein GF14 phi OS=Arabidopsis thalia...	[more]
BMH1_YEAST	4.680e-48	52.94	Protein BMH1 OS=Saccharomyces cerevisiae GN=BMH1 P...	[more]
1433_CANAL	4.680e-48	58.52	14-3-3 protein homolog OS=Candida albicans GN=BMH1...	[more]
14333_SOLLC	6.101e-48	51.77	14-3-3 protein 3 OS=Solanum lycopersicum GN=TFT3 P...	[more]
1433_LILLO	1.037e-47	52.48	14-3-3-like protein OS=Lilium longiflorum PE=2 SV=...	[more]

Pages

Properties


Property Name	Value
Genbank description	USDA-FP_6129 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE59B04 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK739740 ID=CK739740; Name=CK739740; organism=Citrus sinensis; type=EST; length=539bp
AGAGCATCATGGAGGATTTTGTCTTCCATTGAACAGAAGGAGGAGGCCAA
GGGTATGAACAAATGTGAAGAGGATTAAGGAATATAGGCAGAGGGTTGAA
GATGAGCTTGCAAAGATCTGTAATGATATATTGTCTGTCATTGATGAACA
CCTAATCCATCCTCCACATCTGGAGAATCAACCGTGTTTTACTATAAGAT
GAAAGGAGATTATTACCGTTACCTAGCTGAGTTCAAAGGTGCTGATGATC
GTAAGGAAGCTGCAGATCATTCACTTAAGGCTTACGAGGCTGCTACAGCT
GCAGCAGCCTCAGAGTTACCCCCAACTCATCCAATCAGACTTGGCCTGGC
TCTTAACTTTTCTGTTTTCTACTATGAGATTTTGAACTCCCTGAGAGGGC
TTGCCACCTGGCTAAACAAGCATTTGATGAGGCGATTGCTGAACTTGATA
GCCTTAATGAAGAGTCCTACAAGGACAGCACCCTTATTATGCAGCTTCTT
CGGGATAATCTCACCTTGTGGACCTCGGATCTACCGGAG