CK739758

Overview
NameCK739758
Unique NameCK739758
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length550
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK739758 vs. ExPASy Swiss-Prot
Match: M3K12_MOUSE (Mitogen-activated protein kinase kinase kinase 12 OS=Mus musculus GN=Map3k12 PE=1 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 8.169e-19
Identity = 40/110 (36.36%), Postives = 66/110 (60.00%), Query Frame = 2 
Query:   38 EVNLLVKLRHPNIVQFLGAVTERKPLMLITEYLRGGDLHKYLKEKGALSPSTAVNFALDIARGMAYLHNEPNVIIHRDLKPRNVLLVNSSADHLKVGDFGLSKLIKGQNS 367
            ++  L KL+HPNI+ F G  T+     ++ E+   G L++ L+    ++PS  V++++ IA GM YLH   + IIHRDLK  N+L+     D +K+ DFG SK +  +++
Sbjct:  194 DIKHLRKLKHPNIITFKGVCTQAPCYCILMEFCAQGQLYEVLRAGRPVTPSLLVDWSMGIAGGMNYLH--LHKIIHRDLKSPNMLITYD--DVVKISDFGTSKELSDKST 299

HSP 2 Score: 42.3578 bits (98), Expect = 8.169e-19
Identity = 17/38 (44.74%), Postives = 28/38 (73.68%), Query Frame = 1 
Query:  397 GSYRYMAPEVFKHRKYDKKVDVFSFAMILYEMLEGEPP 510
            G+  +MAPEV ++    +KVD++SF ++L+E+L GE P
Sbjct:  305 GTVAWMAPEVIRNEPVSEKVDIWSFGVVLWELLTGEIP 342
[Close]
BLAST of CK739758 vs. ExPASy Swiss-Prot
Match: M3K12_HUMAN (Mitogen-activated protein kinase kinase kinase 12 OS=Homo sapiens GN=MAP3K12 PE=1 SV=2)

HSP 1 Score: 72.0182 bits (175), Expect = 8.174e-19
Identity = 40/110 (36.36%), Postives = 66/110 (60.00%), Query Frame = 2 
Query:   38 EVNLLVKLRHPNIVQFLGAVTERKPLMLITEYLRGGDLHKYLKEKGALSPSTAVNFALDIARGMAYLHNEPNVIIHRDLKPRNVLLVNSSADHLKVGDFGLSKLIKGQNS 367
            ++  L KL+HPNI+ F G  T+     ++ E+   G L++ L+    ++PS  V++++ IA GM YLH   + IIHRDLK  N+L+     D +K+ DFG SK +  +++
Sbjct:  161 DIKHLRKLKHPNIITFKGVCTQAPCYCILMEFCAQGQLYEVLRAGRPVTPSLLVDWSMGIAGGMNYLH--LHKIIHRDLKSPNMLITYD--DVVKISDFGTSKELSDKST 266

HSP 2 Score: 42.3578 bits (98), Expect = 8.174e-19
Identity = 17/38 (44.74%), Postives = 28/38 (73.68%), Query Frame = 1 
Query:  397 GSYRYMAPEVFKHRKYDKKVDVFSFAMILYEMLEGEPP 510
            G+  +MAPEV ++    +KVD++SF ++L+E+L GE P
Sbjct:  272 GTVAWMAPEVIRNEPVSEKVDIWSFGVVLWELLTGEIP 309
[Close]
BLAST of CK739758 vs. ExPASy Swiss-Prot
Match: M3K2_HUMAN (Mitogen-activated protein kinase kinase kinase 2 OS=Homo sapiens GN=MAP3K2 PE=1 SV=2)

HSP 1 Score: 73.559 bits (179), Expect = 8.226e-19
Identity = 41/108 (37.96%), Postives = 62/108 (57.41%), Query Frame = 2 
Query:   38 EVNLLVKLRHPNIVQFLGAVTE--RKPLMLITEYLRGGDLHKYLKEKGALSPSTAVNFALDIARGMAYLHNEPNVIIHRDLKPRNVLLVNSSADHLKVGDFGLSKLIK 355
            E+ LL  L H  IVQ+ G + +   K L +  EY+ GG +   LK  GAL+ +    +   I  G+ YLH+  N+I+HRD+K  N+L    S  ++K+GDFG SK ++
Sbjct:  406 EIQLLKNLLHERIVQYYGCLRDPQEKTLSIFMEYMPGGSIKDQLKAYGALTENVTRKYTRQILEGVHYLHS--NMIVHRDIKGANIL--RDSTGNVKLGDFGASKRLQ 509

HSP 2 Score: 40.817 bits (94), Expect = 8.226e-19
Identity = 20/52 (38.46%), Postives = 29/52 (55.77%), Query Frame = 1 
Query:  382 MTGETGSYRYMAPEVFKHRKYDKKVDVFSFAMILYEMLEGEPPLANYEPYEA 537
            M   TG+  +M+PEV     Y +K D++S A  + EML  +PP A +E   A
Sbjct:  518 MKSVTGTPYWMSPEVISGEGYGRKADIWSVACTVVEMLTEKPPWAEFEAMAA 569
[Close]
BLAST of CK739758 vs. ExPASy Swiss-Prot
Match: Y5148_ARATH (Probable leucine-rich repeat receptor-like protein kinase At5g61480 OS=Arabidopsis thaliana GN=At5g61480 PE=1 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.059e-18
Identity = 51/120 (42.50%), Postives = 72/120 (60.00%), Query Frame = 2 
Query:   38 EVNLLVKLRHPNIVQFLGAVTERKPLMLITEYLRGGDLHKYL----KEKGALSPSTAV-NFALDIARGMAYLHNEPN-VIIHRDLKPRNVLLVNSSAD-HLKVGDFGLSKLIKGQNSHDV 376
            EV++L  +RH NIV+ LG  T R   ML+ EY+  G L   L    K   A +  TA+   A+ +A+G+ YLH++ + VI+HRDLKP N+LL    AD   +V DFG++KLI+   S  V
Sbjct:  769 EVDVLGNVRHRNIVRLLGCCTNRDCTMLLYEYMPNGSLDDLLHGGDKTMTAAAEWTALYQIAIGVAQGICYLHHDCDPVIVHRDLKPSNILL---DADFEARVADFGVAKLIQTDESMSV 885

HSP 2 Score: 37.7354 bits (86), Expect = 1.059e-18
Identity = 17/41 (41.46%), Postives = 27/41 (65.85%), Query Frame = 1 
Query:  382 MTGETGSYRYMAPEVFKHRKYDKKVDVFSFAMILYEMLEGE 504
            M+   GSY Y+APE     + DKK D++S+ +IL E++ G+
Sbjct:  883 MSVVAGSYGYIAPEYAYTLQVDKKSDIYSYGVILLEIITGK 923
[Close]
BLAST of CK739758 vs. ExPASy Swiss-Prot
Match: LIMK2_MOUSE (LIM domain kinase 2 OS=Mus musculus GN=Limk2 PE=1 SV=2)

HSP 1 Score: 77.411 bits (189), Expect = 1.069e-18
Identity = 45/108 (41.67%), Postives = 62/108 (57.41%), Query Frame = 2 
Query:   29 FRHEVNLLVKLRHPNIVQFLGAVTERKPLMLITEYLRGGDLHKYLKEKGALSPSTAVNFALDIARGMAYLHNEPNVIIHRDLKPRNVLLVNSSADHLKVGDFGLSKLI 352
            F  EV ++  L HPN+++F+G + + K L L+TEY+ GG L  +L+          V FA  I+ GMAYLH+    IIHRDL   N L+       + V DFGLS+LI
Sbjct:  373 FLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRSVDPFPWQQKVRFAKGISSGMAYLHS--MCIIHRDLNSHNCLIKLDKT--VVVADFGLSRLI 476

HSP 2 Score: 36.5798 bits (83), Expect = 1.069e-18
Identity = 15/33 (45.45%), Postives = 24/33 (72.73%), Query Frame = 1 
Query:  397 GSYRYMAPEVFKHRKYDKKVDVFSFAMILYEML 495
            G+  +MAPE+   + YD+ VDVFSF ++L E++
Sbjct:  508 GNPYWMAPEMLNGKSYDETVDVFSFGIVLCEII 540
[Close]
BLAST of CK739758 vs. ExPASy Swiss-Prot
Match: M3K7_RAT (Mitogen-activated protein kinase kinase kinase 7 OS=Rattus norvegicus GN=Map3k7 PE=2 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 1.070e-18
Identity = 43/104 (41.35%), Postives = 62/104 (59.62%), Query Frame = 2 
Query:   38 EVNLLVKLRHPNIVQFLGAVTERKPLMLITEYLRGGDLHKYLKEKGALSPSTA---VNFALDIARGMAYLHN-EPNVIIHRDLKPRNVLLVNSSADHLKVGDFG 337
            E+  L ++ HPNIV+  GA     P+ L+ EY  GG L+  L     L   TA   +++ L  ++G+AYLH+ +P  +IHRDLKP N+LLV +    LK+ DFG
Sbjct:   77 ELRQLSRVNHPNIVKLYGACLN--PVCLVMEYAEGGSLYNVLHGAEPLPYYTAAHAMSWCLQCSQGVAYLHSMQPKALIHRDLKPPNLLLV-AGGTVLKICDFG 177

HSP 2 Score: 43.8986 bits (102), Expect = 1.070e-18
Identity = 20/43 (46.51%), Postives = 28/43 (65.12%), Query Frame = 1 
Query:  382 MTGETGSYRYMAPEVFKHRKYDKKVDVFSFAMILYEMLEGEPP 510
            MT   GS  +MAPEVF+   Y +K DVFS+ +IL+E++    P
Sbjct:  186 MTNNKGSAAWMAPEVFEGSNYSEKCDVFSWGIILWEVITRRKP 228
[Close]
BLAST of CK739758 vs. ExPASy Swiss-Prot
Match: M3K7_PONAB (Mitogen-activated protein kinase kinase kinase 7 OS=Pongo abelii GN=MAP3K7 PE=2 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 1.070e-18
Identity = 43/104 (41.35%), Postives = 62/104 (59.62%), Query Frame = 2 
Query:   38 EVNLLVKLRHPNIVQFLGAVTERKPLMLITEYLRGGDLHKYLKEKGALSPSTA---VNFALDIARGMAYLHN-EPNVIIHRDLKPRNVLLVNSSADHLKVGDFG 337
            E+  L ++ HPNIV+  GA     P+ L+ EY  GG L+  L     L   TA   +++ L  ++G+AYLH+ +P  +IHRDLKP N+LLV +    LK+ DFG
Sbjct:   77 ELRQLSRVNHPNIVKLYGACLN--PVCLVMEYAEGGSLYNVLHGAEPLPYYTAAHAMSWCLQCSQGVAYLHSMQPKALIHRDLKPPNLLLV-AGGTVLKICDFG 177

HSP 2 Score: 43.8986 bits (102), Expect = 1.070e-18
Identity = 20/43 (46.51%), Postives = 28/43 (65.12%), Query Frame = 1 
Query:  382 MTGETGSYRYMAPEVFKHRKYDKKVDVFSFAMILYEMLEGEPP 510
            MT   GS  +MAPEVF+   Y +K DVFS+ +IL+E++    P
Sbjct:  186 MTNNKGSAAWMAPEVFEGSNYSEKCDVFSWGIILWEVITRRKP 228
[Close]
BLAST of CK739758 vs. ExPASy Swiss-Prot
Match: M3K7_HUMAN (Mitogen-activated protein kinase kinase kinase 7 OS=Homo sapiens GN=MAP3K7 PE=1 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 1.070e-18
Identity = 43/104 (41.35%), Postives = 62/104 (59.62%), Query Frame = 2 
Query:   38 EVNLLVKLRHPNIVQFLGAVTERKPLMLITEYLRGGDLHKYLKEKGALSPSTA---VNFALDIARGMAYLHN-EPNVIIHRDLKPRNVLLVNSSADHLKVGDFG 337
            E+  L ++ HPNIV+  GA     P+ L+ EY  GG L+  L     L   TA   +++ L  ++G+AYLH+ +P  +IHRDLKP N+LLV +    LK+ DFG
Sbjct:   77 ELRQLSRVNHPNIVKLYGACLN--PVCLVMEYAEGGSLYNVLHGAEPLPYYTAAHAMSWCLQCSQGVAYLHSMQPKALIHRDLKPPNLLLV-AGGTVLKICDFG 177

HSP 2 Score: 43.8986 bits (102), Expect = 1.070e-18
Identity = 20/43 (46.51%), Postives = 28/43 (65.12%), Query Frame = 1 
Query:  382 MTGETGSYRYMAPEVFKHRKYDKKVDVFSFAMILYEMLEGEPP 510
            MT   GS  +MAPEVF+   Y +K DVFS+ +IL+E++    P
Sbjct:  186 MTNNKGSAAWMAPEVFEGSNYSEKCDVFSWGIILWEVITRRKP 228
[Close]
BLAST of CK739758 vs. ExPASy Swiss-Prot
Match: M3K7_MOUSE (Mitogen-activated protein kinase kinase kinase 7 OS=Mus musculus GN=Map3k7 PE=1 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 1.071e-18
Identity = 43/104 (41.35%), Postives = 62/104 (59.62%), Query Frame = 2 
Query:   38 EVNLLVKLRHPNIVQFLGAVTERKPLMLITEYLRGGDLHKYLKEKGALSPSTA---VNFALDIARGMAYLHN-EPNVIIHRDLKPRNVLLVNSSADHLKVGDFG 337
            E+  L ++ HPNIV+  GA     P+ L+ EY  GG L+  L     L   TA   +++ L  ++G+AYLH+ +P  +IHRDLKP N+LLV +    LK+ DFG
Sbjct:   77 ELRQLSRVNHPNIVKLYGACLN--PVCLVMEYAEGGSLYNVLHGAEPLPYYTAAHAMSWCLQCSQGVAYLHSMQPKALIHRDLKPPNLLLV-AGGTVLKICDFG 177

HSP 2 Score: 43.8986 bits (102), Expect = 1.071e-18
Identity = 20/43 (46.51%), Postives = 28/43 (65.12%), Query Frame = 1 
Query:  382 MTGETGSYRYMAPEVFKHRKYDKKVDVFSFAMILYEMLEGEPP 510
            MT   GS  +MAPEVF+   Y +K DVFS+ +IL+E++    P
Sbjct:  186 MTNNKGSAAWMAPEVFEGSNYSEKCDVFSWGIILWEVITRRKP 228
[Close]
BLAST of CK739758 vs. ExPASy Swiss-Prot
Match: M3K7_BOVIN (Mitogen-activated protein kinase kinase kinase 7 OS=Bos taurus GN=MAP3K7 PE=2 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 1.071e-18
Identity = 43/104 (41.35%), Postives = 62/104 (59.62%), Query Frame = 2 
Query:   38 EVNLLVKLRHPNIVQFLGAVTERKPLMLITEYLRGGDLHKYLKEKGALSPSTA---VNFALDIARGMAYLHN-EPNVIIHRDLKPRNVLLVNSSADHLKVGDFG 337
            E+  L ++ HPNIV+  GA     P+ L+ EY  GG L+  L     L   TA   +++ L  ++G+AYLH+ +P  +IHRDLKP N+LLV +    LK+ DFG
Sbjct:   77 ELRQLSRVNHPNIVKLYGACLN--PVCLVMEYAEGGSLYNVLHGAEPLPYYTAAHAMSWCLQCSQGVAYLHSMQPKALIHRDLKPPNLLLV-AGGTVLKICDFG 177

HSP 2 Score: 43.8986 bits (102), Expect = 1.071e-18
Identity = 20/43 (46.51%), Postives = 28/43 (65.12%), Query Frame = 1 
Query:  382 MTGETGSYRYMAPEVFKHRKYDKKVDVFSFAMILYEMLEGEPP 510
            MT   GS  +MAPEVF+   Y +K DVFS+ +IL+E++    P
Sbjct:  186 MTNNKGSAAWMAPEVFEGSNYSEKCDVFSWGIILWEVITRRKP 228
The following BLAST results are available for this feature:
BLAST of CK739758 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
M3K12_MOUSE8.169e-1936.36Mitogen-activated protein kinase kinase kinase 12 ... [more]
M3K12_HUMAN8.174e-1936.36Mitogen-activated protein kinase kinase kinase 12 ... [more]
M3K2_HUMAN8.226e-1937.96Mitogen-activated protein kinase kinase kinase 2 O... [more]
Y5148_ARATH1.059e-1842.50Probable leucine-rich repeat receptor-like protein... [more]
LIMK2_MOUSE1.069e-1841.67LIM domain kinase 2 OS=Mus musculus GN=Limk2 PE=1 ... [more]
M3K7_RAT1.070e-1841.35Mitogen-activated protein kinase kinase kinase 7 O... [more]
M3K7_PONAB1.070e-1841.35Mitogen-activated protein kinase kinase kinase 7 O... [more]
M3K7_HUMAN1.070e-1841.35Mitogen-activated protein kinase kinase kinase 7 O... [more]
M3K7_MOUSE1.071e-1841.35Mitogen-activated protein kinase kinase kinase 7 O... [more]
M3K7_BOVIN1.071e-1841.35Mitogen-activated protein kinase kinase kinase 7 O... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_6148 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE59C11 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK739758 ID=CK739758; Name=CK739758; organism=Citrus sinensis; type=EST; length=550bp
TTCAGATGATAGATTGGTGATTCAGGACTTCAGGCATGAGGTCAATTTGC
TGGTGAAACTTCGCCATCCTAATATAGTCCAATTCCTGGGAGCGGTCACC
GAGAGGAAGCCCCTTATGTTAATTACCGAGTATTTACGAGGGGGTGATCT
TCATAAGTATCTCAAGGAAAAGGGTGCACTTAGTCCTTCAACAGCTGTTA
ACTTTGCACTGGACATTGCCAGAGGCATGGCTTATCTTCACAATGAACCA
AATGTCATAATTCATCGAGACCTGAAGCCAAGGAATGTTCTTCTGGTCAA
CTCAAGTGCTGACCACTTGAAAGTTGGAGATTTTGGACTAAGCAAGCTCA
TCAAGGGTCAGAATTCTCATGATGTGTACAAATGACTGGGGAGACTGGGA
GTTACCGGTATATGGCTCCTGAGGTCTTCAAGCACCGGAAATACGACAAG
AAGGTTGATGTTTTCTCTTTTGCAATGATACTCTATGAGATGCTTGAAGG
AGAACCACCACTTGCAAATTACGAGCCTTATGAAGCAGCAAAATATGTGG
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