CK739913

Overview
NameCK739913
Unique NameCK739913
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length405
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig13176 contig Csv1_Contig13176:750..1153. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CK739913 vs. ExPASy Swiss-Prot
Match: INO2_ARATH (Inositol-3-phosphate synthase isozyme 2 OS=Arabidopsis thaliana GN=At2g22240 PE=2 SV=2)

HSP 1 Score: 200.675 bits (509), Expect = 5.906e-58
Identity = 98/114 (85.96%), Postives = 109/114 (95.61%), Query Frame = 3 
Query:    3 SMVPLPGIYDLDFIAANQGSRANNVIKGTKKEQMLQIIKDIREFKEKNKVDRVVVLWTANTERYSNVIVGLNDTVENLLASLDKNEAEISPSTLYAIACVLENIPFINGSPQNT 344
            +M+PLPGIYD DFIAANQGSRAN+VIKGTKKEQ+  IIKD+REFKEKNKVD++VVLWTANTERYSNVIVGLNDT ENLLAS++K+E+EISPSTLYAIACVLE IPFINGSPQNT
Sbjct:  173 NMIPLPGIYDPDFIAANQGSRANSVIKGTKKEQVDHIIKDMREFKEKNKVDKLVVLWTANTERYSNVIVGLNDTTENLLASVEKDESEISPSTLYAIACVLEGIPFINGSPQNT 286

HSP 2 Score: 43.5134 bits (101), Expect = 5.906e-58
Identity = 18/25 (72.00%), Postives = 22/25 (88.00%), Query Frame = 1 
Query:  331 APKTPFVPGLIDLAIRRNCLIGGDD 405
            +P+  FVPGLI+LAI +NCLIGGDD
Sbjct:  282 SPQNTFVPGLIELAISKNCLIGGDD 306
[Close]
BLAST of CK739913 vs. ExPASy Swiss-Prot
Match: INO1_BRANA (Inositol-3-phosphate synthase OS=Brassica napus PE=2 SV=2)

HSP 1 Score: 202.216 bits (513), Expect = 7.700e-58
Identity = 99/114 (86.84%), Postives = 110/114 (96.49%), Query Frame = 3 
Query:    3 SMVPLPGIYDLDFIAANQGSRANNVIKGTKKEQMLQIIKDIREFKEKNKVDRVVVLWTANTERYSNVIVGLNDTVENLLASLDKNEAEISPSTLYAIACVLENIPFINGSPQNT 344
            ++VPLPGIYD DFIAANQGSRANNVIKGTKKEQ+ QIIKD+REFKEKNKVD+VVVLWTANTERYSNVIVGLNDT+ENL+ S+D++E+EISPSTLYAIACVLE IPFINGSPQNT
Sbjct:  173 NIVPLPGIYDPDFIAANQGSRANNVIKGTKKEQVDQIIKDMREFKEKNKVDKVVVLWTANTERYSNVIVGLNDTMENLMNSVDRDESEISPSTLYAIACVLEGIPFINGSPQNT 286

HSP 2 Score: 41.5874 bits (96), Expect = 7.700e-58
Identity = 18/25 (72.00%), Postives = 21/25 (84.00%), Query Frame = 1 
Query:  331 APKTPFVPGLIDLAIRRNCLIGGDD 405
            +P+  FVPGLIDLAI+ N LIGGDD
Sbjct:  282 SPQNTFVPGLIDLAIKNNVLIGGDD 306
[Close]
BLAST of CK739913 vs. ExPASy Swiss-Prot
Match: INO1_ARATH (Inositol-3-phosphate synthase isozyme 1 OS=Arabidopsis thaliana GN=At4g39800 PE=2 SV=3)

HSP 1 Score: 197.208 bits (500), Expect = 1.857e-56
Identity = 95/114 (83.33%), Postives = 109/114 (95.61%), Query Frame = 3 
Query:    3 SMVPLPGIYDLDFIAANQGSRANNVIKGTKKEQMLQIIKDIREFKEKNKVDRVVVLWTANTERYSNVIVGLNDTVENLLASLDKNEAEISPSTLYAIACVLENIPFINGSPQNT 344
            ++VPLPGI+D DFIAANQGSRAN+VIKGTKKEQ+  IIKD+REFKEKNKVD+VVVLWTANTERYSNV+VG+NDT+ENL+ S+D++EAEISPSTLYAIACVLE IPFINGSPQNT
Sbjct:  174 NIVPLPGIFDPDFIAANQGSRANHVIKGTKKEQVDHIIKDMREFKEKNKVDKVVVLWTANTERYSNVVVGMNDTMENLMESVDRDEAEISPSTLYAIACVLEGIPFINGSPQNT 287

HSP 2 Score: 41.9726 bits (97), Expect = 1.857e-56
Identity = 18/25 (72.00%), Postives = 21/25 (84.00%), Query Frame = 1 
Query:  331 APKTPFVPGLIDLAIRRNCLIGGDD 405
            +P+  FVPGLID+AIR N LIGGDD
Sbjct:  283 SPQNTFVPGLIDMAIRNNVLIGGDD 307
[Close]
BLAST of CK739913 vs. ExPASy Swiss-Prot
Match: INO1_PHAVU (Inositol-3-phosphate synthase OS=Phaseolus vulgaris PE=2 SV=1)

HSP 1 Score: 191.045 bits (484), Expect = 2.196e-54
Identity = 92/114 (80.70%), Postives = 108/114 (94.74%), Query Frame = 3 
Query:    3 SMVPLPGIYDLDFIAANQGSRANNVIKGTKKEQMLQIIKDIREFKEKNKVDRVVVLWTANTERYSNVIVGLNDTVENLLASLDKNEAEISPSTLYAIACVLENIPFINGSPQNT 344
            ++VPLPGI+D DFIAANQGSRAN+VIKGTKKEQ+  IIKD+REFKEKNKVD+VVVLWTANTERYSN +VG+NDT+E+L+ S+D++EAEISPSTLYAIACVLE IPFINGSPQ+T
Sbjct:  174 NIVPLPGIFDPDFIAANQGSRANHVIKGTKKEQVDHIIKDMREFKEKNKVDKVVVLWTANTERYSNEVVGMNDTMEDLMESVDRDEAEISPSTLYAIACVLEGIPFINGSPQHT 287

HSP 2 Score: 41.2022 bits (95), Expect = 2.196e-54
Identity = 18/25 (72.00%), Postives = 21/25 (84.00%), Query Frame = 1 
Query:  331 APKTPFVPGLIDLAIRRNCLIGGDD 405
            +P+  FVPGLID+AIR N LIGGDD
Sbjct:  283 SPQHTFVPGLIDMAIRNNVLIGGDD 307
[Close]
BLAST of CK739913 vs. ExPASy Swiss-Prot
Match: INO1_SPIPO (Inositol-3-phosphate synthase OS=Spirodela polyrrhiza GN=TUR1 PE=2 SV=1)

HSP 1 Score: 186.422 bits (472), Expect = 5.289e-53
Identity = 86/114 (75.44%), Postives = 108/114 (94.74%), Query Frame = 3 
Query:    3 SMVPLPGIYDLDFIAANQGSRANNVIKGTKKEQMLQIIKDIREFKEKNKVDRVVVLWTANTERYSNVIVGLNDTVENLLASLDKNEAEISPSTLYAIACVLENIPFINGSPQNT 344
            SMVPLPGIY+ DFIAANQGSRANNVIKG KK+Q+ +II DIREFKE+ KV++VVVLWTANTERYS+++VGLNDT+ENLLA+++++EAEISPS+LYA+AC++E +PF+NGSPQNT
Sbjct:  173 SMVPLPGIYNPDFIAANQGSRANNVIKGPKKQQVQRIIDDIREFKEREKVEKVVVLWTANTERYSDLVVGLNDTMENLLAAVERDEAEISPSSLYALACIMEGVPFVNGSPQNT 286

HSP 2 Score: 41.2022 bits (95), Expect = 5.289e-53
Identity = 17/25 (68.00%), Postives = 22/25 (88.00%), Query Frame = 1 
Query:  331 APKTPFVPGLIDLAIRRNCLIGGDD 405
            +P+  FVPGLI++AI+RN LIGGDD
Sbjct:  282 SPQNTFVPGLIEMAIKRNSLIGGDD 306
[Close]
BLAST of CK739913 vs. ExPASy Swiss-Prot
Match: INO1_DICDI (Inositol-3-phosphate synthase OS=Dictyostelium discoideum GN=ino1 PE=3 SV=1)

HSP 1 Score: 162.925 bits (411), Expect = 3.833e-44
Identity = 76/114 (66.67%), Postives = 98/114 (85.96%), Query Frame = 3 
Query:    3 SMVPLPGIYDLDFIAANQGSRANNVIKGTKKEQMLQIIKDIREFKEKNKVDRVVVLWTANTERYSNVIVGLNDTVENLLASLDKNEAEISPSTLYAIACVLENIPFINGSPQNT 344
            ++ PLP IY  DFIAANQ  RANNV+ GTKKEQM QI KDIR+FKE NK+D VVV+W+ANTER+S+++ G+NDT+ENL+A++D++E EISPSTL+A+A +LEN  +INGSPQNT
Sbjct:  173 NITPLPSIYFPDFIAANQKDRANNVLTGTKKEQMEQIRKDIRDFKESNKLDTVVVMWSANTERFSSLVPGVNDTIENLMAAIDRSEEEISPSTLFAVASILENTTYINGSPQNT 286

HSP 2 Score: 35.039 bits (79), Expect = 3.833e-44
Identity = 14/25 (56.00%), Postives = 19/25 (76.00%), Query Frame = 1 
Query:  331 APKTPFVPGLIDLAIRRNCLIGGDD 405
            +P+  FVP ++DLAI+ N  IGGDD
Sbjct:  282 SPQNTFVPAVVDLAIQHNVSIGGDD 306
[Close]
BLAST of CK739913 vs. ExPASy Swiss-Prot
Match: INO1_DROME (Inositol-3-phosphate synthase OS=Drosophila melanogaster GN=Inos PE=1 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 8.662e-37
Identity = 67/111 (60.36%), Postives = 88/111 (79.28%), Query Frame = 3 
Query:   12 PLPGIYDLDFIAANQGSRANNVIKGTKKEQMLQIIKDIREFKEKNKVDRVVVLWTANTERYSNVIVGLNDTVENLLASLDKNEAEISPSTLYAIACVLENIPFINGSPQNT 344
            P P IYD DFIAANQ  RA+NVI+GT+ EQ  QI KDIR+F+E++ VD V+VLWTANTER+++V  GLN T + L+ASL+ N +E+SPST++A+A + E   +INGSPQNT
Sbjct:  177 PRPSIYDPDFIAANQSDRADNVIRGTRLEQYEQIRKDIRDFRERSGVDSVIVLWTANTERFADVQPGLNTTSQELIASLEANHSEVSPSTIFAMASIAEGCTYINGSPQNT 287

HSP 2 Score: 32.7278 bits (73), Expect = 8.662e-37
Identity = 14/25 (56.00%), Postives = 17/25 (68.00%), Query Frame = 1 
Query:  331 APKTPFVPGLIDLAIRRNCLIGGDD 405
            +P+  FVPGLI LA  +N  I GDD
Sbjct:  283 SPQNTFVPGLIQLAEEKNVFIAGDD 307
[Close]
BLAST of CK739913 vs. ExPASy Swiss-Prot
Match: INO1_RAT (Inositol-3-phosphate synthase 1 OS=Rattus norvegicus GN=Isyna1 PE=2 SV=2)

HSP 1 Score: 129.413 bits (324), Expect = 9.013e-33
Identity = 63/115 (54.78%), Postives = 86/115 (74.78%), Query Frame = 3 
Query:    3 SMVPLPGIYDLDFIAANQGSRANNVIKGTKKEQMLQIIKDIREFKEKNKVDRVVVLWTANTERYSNVIVGLNDTVENLLASLDKNEAEISPSTLYAIACVLENIPFINGSPQNTL 347
            S+ P P +Y  +FIAANQ +RA+N+I GT+ +Q+ QI KDIR+F+    +D+V+VLWTANTER+  V+ G NDT ENLL ++     E+SPSTL+A+A +LE   F+NGSPQNTL
Sbjct:  171 SLRPRPSVYIPEFIAANQTARADNLIPGTRAQQLEQIRKDIRDFRSSAGLDKVIVLWTANTERFCEVVPGRNDTAENLLRTIQLG-LEVSPSTLFAVASILEGCAFLNGSPQNTL 284

HSP 2 Score: 30.4166 bits (67), Expect = 9.013e-33
Identity = 11/25 (44.00%), Postives = 18/25 (72.00%), Query Frame = 1 
Query:  331 APKTPFVPGLIDLAIRRNCLIGGDD 405
            +P+   VPG ++LA +R+  +GGDD
Sbjct:  279 SPQNTLVPGALELASQRHVFVGGDD 303
[Close]
BLAST of CK739913 vs. ExPASy Swiss-Prot
Match: INO1_MOUSE (Inositol-3-phosphate synthase 1 OS=Mus musculus GN=Isyna1 PE=2 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 9.013e-33
Identity = 63/115 (54.78%), Postives = 87/115 (75.65%), Query Frame = 3 
Query:    3 SMVPLPGIYDLDFIAANQGSRANNVIKGTKKEQMLQIIKDIREFKEKNKVDRVVVLWTANTERYSNVIVGLNDTVENLLASLDKNEAEISPSTLYAIACVLENIPFINGSPQNTL 347
            S+ P P +Y  +FIAANQ +RA+N+I GT+ +Q+ QI KDIR+F+    +D+V+VLWTANTER+  V+ G NDT ENLL ++     E+SPSTL+A+A +LE+  F+NGSPQNTL
Sbjct:  171 SLRPRPSVYIPEFIAANQTARADNLIPGTRAQQLEQIRKDIRDFRSSAGLDKVIVLWTANTERFCEVVPGRNDTAENLLHTIQLG-LEVSPSTLFAVASILEDCAFLNGSPQNTL 284

HSP 2 Score: 30.4166 bits (67), Expect = 9.013e-33
Identity = 11/25 (44.00%), Postives = 18/25 (72.00%), Query Frame = 1 
Query:  331 APKTPFVPGLIDLAIRRNCLIGGDD 405
            +P+   VPG ++LA +R+  +GGDD
Sbjct:  279 SPQNTLVPGALELASQRHVFVGGDD 303
[Close]
BLAST of CK739913 vs. ExPASy Swiss-Prot
Match: INO1_CANAL (Inositol-3-phosphate synthase OS=Candida albicans GN=INO1 PE=1 SV=1)

HSP 1 Score: 127.102 bits (318), Expect = 1.530e-32
Identity = 61/119 (51.26%), Postives = 85/119 (71.43%), Query Frame = 3 
Query:   12 PLPGIYDLDFIAANQGSRANNVIKGTKKE--------QMLQIIKDIREFKEKNKVDRVVVLWTANTERYSNVIVGLNDTVENLLASLDKNEAEISPSTLYAIACVLENIPFINGSPQNT 344
            PL  IY  DFIA NQ  RANNV      E         + +I KDIR+FK KN++D+V++LWTANTERY++V+  +NDT +NL+ S+ ++  EI+PST++A+A +LE +P+INGSPQNT
Sbjct:  174 PLESIYYPDFIALNQSERANNVFNQVNGEVKTDNKWADVEKIRKDIRDFKAKNELDKVIILWTANTERYADVLPNVNDTADNLIKSIKESHEEIAPSTVFAVASILEKVPYINGSPQNT 292

HSP 2 Score: 31.9574 bits (71), Expect = 1.530e-32
Identity = 13/25 (52.00%), Postives = 19/25 (76.00%), Query Frame = 1 
Query:  331 APKTPFVPGLIDLAIRRNCLIGGDD 405
            +P+  FVPG+I+LA + +  IGGDD
Sbjct:  288 SPQNTFVPGVIELAEKYDSFIGGDD 312
The following BLAST results are available for this feature:
BLAST of CK739913 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 27
Match NameE-valueIdentityDescription
INO2_ARATH5.906e-5885.96Inositol-3-phosphate synthase isozyme 2 OS=Arabido... [more]
INO1_BRANA7.700e-5886.84Inositol-3-phosphate synthase OS=Brassica napus PE... [more]
INO1_ARATH1.857e-5683.33Inositol-3-phosphate synthase isozyme 1 OS=Arabido... [more]
INO1_PHAVU2.196e-5480.70Inositol-3-phosphate synthase OS=Phaseolus vulgari... [more]
INO1_SPIPO5.289e-5375.44Inositol-3-phosphate synthase OS=Spirodela polyrrh... [more]
INO1_DICDI3.833e-4466.67Inositol-3-phosphate synthase OS=Dictyostelium dis... [more]
INO1_DROME8.662e-3760.36Inositol-3-phosphate synthase OS=Drosophila melano... [more]
INO1_RAT9.013e-3354.78Inositol-3-phosphate synthase 1 OS=Rattus norvegic... [more]
INO1_MOUSE9.013e-3354.78Inositol-3-phosphate synthase 1 OS=Mus musculus GN... [more]
INO1_CANAL1.530e-3251.26Inositol-3-phosphate synthase OS=Candida albicans ... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_6305 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE61G09 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK739913 ID=CK739913; Name=CK739913; organism=Citrus sinensis; type=EST; length=405bp
AATCAATGGTCCCACTTCCTGGAATCTATGACCTTGATTTCATTGCTGCC
AATCAAGGGTCCCGTGCCAACAACGTGATCAAAGGAACCAAGAAAGAACA
AATGCTACAAATCATCAAAGATATTAGAGAGTTTAAGGAGAAAAACAAGG
TGGATAGGGTTGTTGTACTGTGGACTGCTAACACTGAGAGGTACAGTAAT
GTGATTGTTGGGCTAAATGACACAGTGGAGAACCTTCTTGCTTCACTGGA
CAAAAACGAAGCTGAGATTTCTCCTTCCACCTTGTATGCTATTGCTTGTG
TTCTTGAAAATATCCCTTTCATCAATGGAAGCCCCCAAAACACCCTTTGT
ACCAGGGCTTATTGACTTGGCTATTAGGAGGAACTGTTTGATTGGTGGAG
ATGAC
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