CK740044

Overview
NameCK740044
Unique NameCK740044
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length348
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig3336 contig Csv1_Contig3336:567..914. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CK740044 vs. ExPASy Swiss-Prot
Match: SUCD_STAAN (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Staphylococcus aureus (strain N315) GN=sucD PE=1 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 9.900e-32
Identity = 66/108 (61.11%), Postives = 75/108 (69.44%), Query Frame = 2 
Query:   14 KTRLIGPNCPGIIKPGECKIGIMPGYIHKPGRVGIVSRSGTLTYEAVFQTTVVGLGQSTCVGIGGDPFNGTNFVDCLEKFLVDPQTXXXXXXXXXXXXXXXDAAALIK 337
            KTRL+GPNCPG+I   ECKIGIMPGYIHK G VG+VSRSGTLTYEAV Q T  G+GQ+T VGIGGDP NGTNF+D L+ F  D +T               +AA  IK
Sbjct:  116 KTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVVSRSGTLTYEAVHQLTEEGIGQTTAVGIGGDPVNGTNFIDVLKAFNEDDETKAVVMIGEIGGTAEEEAAEWIK 223
[Close]
BLAST of CK740044 vs. ExPASy Swiss-Prot
Match: SUCD_STAAM (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) GN=sucD PE=1 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 9.900e-32
Identity = 66/108 (61.11%), Postives = 75/108 (69.44%), Query Frame = 2 
Query:   14 KTRLIGPNCPGIIKPGECKIGIMPGYIHKPGRVGIVSRSGTLTYEAVFQTTVVGLGQSTCVGIGGDPFNGTNFVDCLEKFLVDPQTXXXXXXXXXXXXXXXDAAALIK 337
            KTRL+GPNCPG+I   ECKIGIMPGYIHK G VG+VSRSGTLTYEAV Q T  G+GQ+T VGIGGDP NGTNF+D L+ F  D +T               +AA  IK
Sbjct:  116 KTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVVSRSGTLTYEAVHQLTEEGIGQTTAVGIGGDPVNGTNFIDVLKAFNEDDETKAVVMIGEIGGTAEEEAAEWIK 223
[Close]
BLAST of CK740044 vs. ExPASy Swiss-Prot
Match: SUCD_STAAC (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Staphylococcus aureus (strain COL) GN=sucD PE=3 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 9.900e-32
Identity = 66/108 (61.11%), Postives = 75/108 (69.44%), Query Frame = 2 
Query:   14 KTRLIGPNCPGIIKPGECKIGIMPGYIHKPGRVGIVSRSGTLTYEAVFQTTVVGLGQSTCVGIGGDPFNGTNFVDCLEKFLVDPQTXXXXXXXXXXXXXXXDAAALIK 337
            KTRL+GPNCPG+I   ECKIGIMPGYIHK G VG+VSRSGTLTYEAV Q T  G+GQ+T VGIGGDP NGTNF+D L+ F  D +T               +AA  IK
Sbjct:  116 KTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVVSRSGTLTYEAVHQLTEEGIGQTTAVGIGGDPVNGTNFIDVLKAFNEDDETKAVVMIGEIGGTAEEEAAEWIK 223
[Close]
BLAST of CK740044 vs. ExPASy Swiss-Prot
Match: SUCD_AQUAE (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Aquifex aeolicus GN=sucD PE=3 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 1.479e-27
Identity = 55/84 (65.48%), Postives = 66/84 (78.57%), Query Frame = 2 
Query:   20 RLIGPNCPGIIKPGECKIGIMPGYIHKPGRVGIVSRSGTLTYEAVFQTTVVGLGQSTCVGIGGDPFNGTNFVDCLEKFLVDPQT 271
            +L+GPNCPG+I PGE K+GIMPG+I K G++GIVSRSGTLTYEA +Q T  GLGQST VGIGGDP +G    D +E F  DP+T
Sbjct:  120 KLVGPNCPGVITPGEAKVGIMPGHIFKRGKIGIVSRSGTLTYEAAYQLTKYGLGQSTAVGIGGDPVHGLTHRDVIEMFNKDPET 203
[Close]
BLAST of CK740044 vs. ExPASy Swiss-Prot
Match: MTKB_METEA (Malate--CoA ligase subunit alpha OS=Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1) GN=mtkB PE=3 SV=2)

HSP 1 Score: 115.931 bits (289), Expect = 6.215e-26
Identity = 55/113 (48.67%), Postives = 76/113 (67.26%), Query Frame = 2 
Query:    5 RQSKTRLIGPNCPGIIKPGECKIGIMPGYIHKPGRVGIVSRSGTLTYEAVFQTTVVGLGQSTCVGIGGDPFNGTNFVDCLEKFLVDPQTXXXXXXXXXXXXXXXDAAALIKES 343
            ++ +T ++GPNC GII PG+  +GIMPG+I+ PG+VG++SRSGTL YEA  Q   +G+G ST VGIGGDP NG++F+D L  F  DP+T               +A+A IKE+
Sbjct:  117 KEKRTMVVGPNCAGIISPGKSMLGIMPGHIYLPGKVGVISRSGTLGYEAAAQMKELGIGISTSVGIGGDPINGSSFLDHLALFEQDPETEAVLMIGEIGGPQEAEASAWIKEN 229
[Close]
BLAST of CK740044 vs. ExPASy Swiss-Prot
Match: SUCA_YEAST (Succinyl-CoA ligase [ADP-forming] subunit alpha, mitochondrial OS=Saccharomyces cerevisiae GN=LSC1 PE=1 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 6.872e-25
Identity = 58/112 (51.79%), Postives = 72/112 (64.29%), Query Frame = 2 
Query:    8 QSKTRLIGPNCPGIIKPG-ECKIGIMPGYIHKPGRVGIVSRSGTLTYEAVFQTTVVGLGQSTCVGIGGDPFNGTNFVDCLEKFLVDPQTXXXXXXXXXXXXXXXDAAALIKE 340
            Q KTRL+GPNCPGII P  + +IGI P  I + G++GI+SRSGTLTYEAV QTT   LGQS  +G+GGD F GT+F+D L+ FL D  T               +AA  +KE
Sbjct:  143 QDKTRLVGPNCPGIINPATKVRIGIQPPKIFQAGKIGIISRSGTLTYEAVQQTTKTDLGQSLVIGMGGDAFPGTDFIDALKLFLEDETTEGIIMLGEIGGKAEIEAAQFLKE 254
[Close]
BLAST of CK740044 vs. ExPASy Swiss-Prot
Match: SUCA3_TRIVA (Succinyl-CoA ligase [GDP-forming] subunit alpha-3, mitochondrial OS=Trichomonas vaginalis GN=ALPHA-SCS3 PE=3 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 8.975e-25
Identity = 57/114 (50.00%), Postives = 74/114 (64.91%), Query Frame = 2 
Query:    5 RQSKTRLIGPNCPGIIKPG-ECKIGIMPGYIHKPGRVGIVSRSGTLTYEAVFQTTVVGLGQSTCVGIGGDPFNGTNFVDCLEKFLVDPQTXXXXXXXXXXXXXXXDAAALIKES 343
            +++  +LIGPNCPG+I+PG   K+GI+P  I   G++GIVSRSGTLTYEA + TT+ GLGQST VGIGGDPF G    D +++F  DPQT               DAA  I ++
Sbjct:  124 KETGCQLIGPNCPGLIQPGTHTKLGIIPTNIFNNGKIGIVSRSGTLTYEAAYATTLAGLGQSTVVGIGGDPFAGQLHTDVVKRFAADPQTEGIILIGEIGGTSEEDAAEWIAKT 237
[Close]
BLAST of CK740044 vs. ExPASy Swiss-Prot
Match: SUCA2_TRIVA (Succinyl-CoA ligase [GDP-forming] subunit alpha-2, mitochondrial OS=Trichomonas vaginalis GN=ALPHA-SCS2 PE=3 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 8.975e-25
Identity = 57/114 (50.00%), Postives = 74/114 (64.91%), Query Frame = 2 
Query:    5 RQSKTRLIGPNCPGIIKPG-ECKIGIMPGYIHKPGRVGIVSRSGTLTYEAVFQTTVVGLGQSTCVGIGGDPFNGTNFVDCLEKFLVDPQTXXXXXXXXXXXXXXXDAAALIKES 343
            +++  +LIGPNCPG+I+PG   K+GI+P  I + G++GIVSRSGTLTYEA + TT  GLGQST VGIGGDPF G    D +++F  DPQT               DAA  I ++
Sbjct:  124 KETGCQLIGPNCPGLIQPGTHTKLGIIPTNIFRNGKIGIVSRSGTLTYEAAYATTQAGLGQSTVVGIGGDPFAGQLHTDVIKRFAADPQTEGIILIGEIGGTSEEDAAEWIAKT 237
[Close]
BLAST of CK740044 vs. ExPASy Swiss-Prot
Match: SUCA1_TRIVA (Succinyl-CoA ligase [GDP-forming] subunit alpha-1, mitochondrial OS=Trichomonas vaginalis GN=ALPHA-SCS1 PE=3 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 8.975e-25
Identity = 57/114 (50.00%), Postives = 74/114 (64.91%), Query Frame = 2 
Query:    5 RQSKTRLIGPNCPGIIKPG-ECKIGIMPGYIHKPGRVGIVSRSGTLTYEAVFQTTVVGLGQSTCVGIGGDPFNGTNFVDCLEKFLVDPQTXXXXXXXXXXXXXXXDAAALIKES 343
            +++  +LIGPNCPG+I+PG   K+GI+P  I   G++GIVSRSGTLTYEA + TT+ GLGQST VGIGGDPF G    D +++F  DPQT               DAA  I ++
Sbjct:  124 KETGCQLIGPNCPGLIQPGTHTKLGIIPTNIFNNGKIGIVSRSGTLTYEAAYATTLAGLGQSTVVGIGGDPFAGQLHTDVVKRFAADPQTEGIILIGEIGGTSEEDAAEWIAKT 237
[Close]
BLAST of CK740044 vs. ExPASy Swiss-Prot
Match: SUCD_THETH (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Thermus thermophilus GN=sucD PE=1 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 2.611e-24
Identity = 58/108 (53.70%), Postives = 68/108 (62.96%), Query Frame = 2 
Query:   17 TRLIGPNCPGIIKPGECKIGIMPGYIHKPGRVGIVSRSGTLTYEAVFQTTVVGLGQSTCVGIGGDPFNGTNFVDCLEKFLVDPQTXXXXXXXXXXXXXXXDAAALIKE 340
            +RLIG NCPGII   E KIGIMPG++ K GRVGI+SRSGTLTYEA    +  GLG +T VGIGGDP  GT F D L  F  DP+T               +AAA +K+
Sbjct:  116 SRLIGGNCPGIISAEETKIGIMPGHVFKRGRVGIISRSGTLTYEAAAALSQAGLGTTTTVGIGGDPVIGTTFKDLLPLFNEDPETEAVVLIGEIGGSDEEEAAAWVKD 223
The following BLAST results are available for this feature:
BLAST of CK740044 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 46
Match NameE-valueIdentityDescription
SUCD_STAAN9.900e-3261.11Succinyl-CoA ligase [ADP-forming] subunit alpha OS... [more]
SUCD_STAAM9.900e-3261.11Succinyl-CoA ligase [ADP-forming] subunit alpha OS... [more]
SUCD_STAAC9.900e-3261.11Succinyl-CoA ligase [ADP-forming] subunit alpha OS... [more]
SUCD_AQUAE1.479e-2765.48Succinyl-CoA ligase [ADP-forming] subunit alpha OS... [more]
MTKB_METEA6.215e-2648.67Malate--CoA ligase subunit alpha OS=Methylobacteri... [more]
SUCA_YEAST6.872e-2551.79Succinyl-CoA ligase [ADP-forming] subunit alpha, m... [more]
SUCA3_TRIVA8.975e-2550.00Succinyl-CoA ligase [GDP-forming] subunit alpha-3,... [more]
SUCA2_TRIVA8.975e-2550.00Succinyl-CoA ligase [GDP-forming] subunit alpha-2,... [more]
SUCA1_TRIVA8.975e-2550.00Succinyl-CoA ligase [GDP-forming] subunit alpha-1,... [more]
SUCD_THETH2.611e-2453.70Succinyl-CoA ligase [ADP-forming] subunit alpha OS... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_6538 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE64G06 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK740044 ID=CK740044; Name=CK740044; organism=Citrus sinensis; type=EST; length=348bp
AAACAGGCAATCTAAAACTCGGCTGATTGGTCCCAACTGCCCTGGTATCA
TCAAACCTGGAGAATGTAAGATTGGCATTATGCCTGGGTATATTCACAAA
CCTGGGCGGGTTGGAATTGTTTCTCGATCTGGTACCTTGACCTACGAAGC
AGTTTTTCAAACAACTGTAGTTGGCCTTGGACAATCAACCTGTGTAGGTA
TAGGTGGGGATCCTTTTAATGGGACAAACTTTGTTGATTGCCTTGAAAAG
TTCCTAGTTGATCCCCAGACAGAAGGTATTATTCTAATAGGAGAGATCGG
TGGGACTGCAGAAGAGGATGCCGCTGCCTTGATAAAGGAAAGTGGGAC
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