CK740108

Overview
NameCK740108
Unique NameCK740108
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length623
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK740108 vs. ExPASy Swiss-Prot
Match: CH601_ANAVT (60 kDa chaperonin 1 OS=Anabaena variabilis (strain ATCC 29413 / PCC 7937) GN=groL1 PE=3 SV=1)

HSP 1 Score: 160.999 bits (406), Expect = 1.431e-43
Identity = 84/170 (49.41%), Postives = 118/170 (69.41%), Query Frame = 2 
Query:  110 RKVTIRKDSTTIIADAASKDEIQARIAQLKKELAETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSDHVPAIKDKLEDADERLGADIVQKALVAPASLIAHNAXXXXXXXXXKVKDSEWTTGYNAMTDKYENM 619
            R++TI KDSTTI+A+  +   ++ R+ Q+++++ ET+S YD EKL ER+AKLSGGVAV+KVGAATETE++D+KLR+EDA NAT AA+EEGIVPGGG  L HL+  +    +     +E  GA IV +AL AP   IA NA         +VK+  +  G+NA T+++ +M
Sbjct:  320 RRITITKDSTTIVAEG-NDVAVKGRVEQIRRQMEETESSYDKEKLQERLAKLSGGVAVVKVGAATETEMKDKKLRLEDAINATKAAVEEGIVPGGGTTLAHLTPELEVWANSNLKDEELTGALIVARALPAPLKRIAENAGQNGAVIAERVKEKAFNVGFNAATNEFVDM 488

HSP 2 Score: 35.8094 bits (81), Expect = 1.431e-43
Identity = 16/30 (53.33%), Postives = 22/30 (73.33%), Query Frame = 3 
Query:    3 KALLQDIAIVTGAEFQAGDLGLLIENTSVE 92
            KA+L+DIAI+TG +    D GL +ENT +E
Sbjct:  285 KAMLEDIAILTGGQLITEDAGLKLENTKLE 314
[Close]
BLAST of CK740108 vs. ExPASy Swiss-Prot
Match: CH601_ANASP (60 kDa chaperonin 1 OS=Anabaena sp. (strain PCC 7120) GN=groL1 PE=3 SV=1)

HSP 1 Score: 160.999 bits (406), Expect = 1.431e-43
Identity = 84/170 (49.41%), Postives = 118/170 (69.41%), Query Frame = 2 
Query:  110 RKVTIRKDSTTIIADAASKDEIQARIAQLKKELAETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSDHVPAIKDKLEDADERLGADIVQKALVAPASLIAHNAXXXXXXXXXKVKDSEWTTGYNAMTDKYENM 619
            R++TI KDSTTI+A+  +   ++ R+ Q+++++ ET+S YD EKL ER+AKLSGGVAV+KVGAATETE++D+KLR+EDA NAT AA+EEGIVPGGG  L HL+  +    +     +E  GA IV +AL AP   IA NA         +VK+  +  G+NA T+++ +M
Sbjct:  320 RRITITKDSTTIVAEG-NDVAVKGRVEQIRRQMEETESSYDKEKLQERLAKLSGGVAVVKVGAATETEMKDKKLRLEDAINATKAAVEEGIVPGGGTTLAHLTPELEVWANSNLKDEELTGALIVARALPAPLKRIAENAGQNGAVIAERVKEKAFNVGFNAATNEFVDM 488

HSP 2 Score: 35.8094 bits (81), Expect = 1.431e-43
Identity = 16/30 (53.33%), Postives = 22/30 (73.33%), Query Frame = 3 
Query:    3 KALLQDIAIVTGAEFQAGDLGLLIENTSVE 92
            KA+L+DIAI+TG +    D GL +ENT +E
Sbjct:  285 KAMLEDIAILTGGQLITEDAGLKLENTKLE 314
[Close]
BLAST of CK740108 vs. ExPASy Swiss-Prot
Match: CH60_EMIHU (60 kDa chaperonin, chloroplastic OS=Emiliania huxleyi GN=groL PE=3 SV=1)

HSP 1 Score: 164.466 bits (415), Expect = 1.432e-43
Identity = 87/170 (51.18%), Postives = 121/170 (71.18%), Query Frame = 2 
Query:  110 RKVTIRKDSTTIIADAASKDEIQARIAQLKKELAETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSDHVPAIKDKLEDADERLGADIVQKALVAPASLIAHNAXXXXXXXXXKVKDSEWTTGYNAMTDKYENM 619
            R+V + K++TTII+D+ +K E+ AR  QL++++  +DS Y+ EKL ER+AKL+GGVAVIKVGAATETE++DRKLR+EDA NAT AA+EEGIVPGGG  L+H++  +     +    DE LG  IV+KAL AP   IA NA         ++K+S++  GYNA T++  +M
Sbjct:  322 RRVVVGKENTTIISDS-NKQEVLARCEQLRRQMETSDSTYEKEKLQERLAKLTGGVAVIKVGAATETEMKDRKLRLEDAVNATKAAVEEGIVPGGGTTLIHIAAELLEWVKETLTGDELLGGLIVEKALQAPLKKIALNAGENGSIIVERIKESDFEIGYNAATNEIVDM 490

HSP 2 Score: 32.3426 bits (72), Expect = 1.432e-43
Identity = 13/30 (43.33%), Postives = 20/30 (66.67%), Query Frame = 3 
Query:    3 KALLQDIAIVTGAEFQAGDLGLLIENTSVE 92
            KA+LQD+A++TG +    D GL +E   +E
Sbjct:  287 KAILQDLAVLTGGDVITADAGLSLERMDIE 316
[Close]
BLAST of CK740108 vs. ExPASy Swiss-Prot
Match: CH602_PROMP (60 kDa chaperonin 2 OS=Prochlorococcus marinus subsp. pastoris (strain CCMP1986 / MED4) GN=groL2 PE=3 SV=1)

HSP 1 Score: 166.007 bits (419), Expect = 1.865e-43
Identity = 88/170 (51.76%), Postives = 116/170 (68.24%), Query Frame = 2 
Query:  110 RKVTIRKDSTTIIADAASKDEIQARIAQLKKELAETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSDHVPAIKDKLEDADERLGADIVQKALVAPASLIAHNAXXXXXXXXXKVKDSEWTTGYNAMTDKYENM 619
            R++TI K++TTI+A+   K  + AR  Q+KK++ ETDS YD EKL ER+AKL+GGVAVIKVGAATETE++D+KLR+EDA NAT AA+EEGIVPGGG  L HL+  +    DK    +E +GA+IV+ +L +P   IA NA          VK   +  G+NA T  Y +M
Sbjct:  320 RRITINKETTTIVAEGNEK-AVTARCDQIKKQMDETDSSYDKEKLQERLAKLAGGVAVIKVGAATETEMKDKKLRLEDAINATKAAVEEGIVPGGGTTLAHLAPILKEWADKALSGEELIGANIVEASLTSPLMRIAENAGSNGAVIAENVKSKPFNDGFNAATGDYVDM 488

HSP 2 Score: 30.4166 bits (67), Expect = 1.865e-43
Identity = 12/30 (40.00%), Postives = 21/30 (70.00%), Query Frame = 3 
Query:    3 KALLQDIAIVTGAEFQAGDLGLLIENTSVE 92
            KA+L+D+A++T  +    D GL +EN ++E
Sbjct:  285 KAMLEDMAVLTNGQLITEDAGLKLENATLE 314
[Close]
BLAST of CK740108 vs. ExPASy Swiss-Prot
Match: CH602_SYNPW (60 kDa chaperonin 2 OS=Synechococcus sp. (strain WH7803) GN=groL2 PE=3 SV=1)

HSP 1 Score: 165.236 bits (417), Expect = 2.430e-43
Identity = 89/171 (52.05%), Postives = 115/171 (67.25%), Query Frame = 2 
Query:  110 RKVTIRKDSTTIIADAASKDEIQARIAQLKKELAETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSDHVPAIKDKLEDADERLGADIVQKALVAPASLIAHNAXXXXXXXXXKVKDSEWTTGYNAMTDKYENML 622
            R+VTI KD+TTI+A+  ++  +QAR  Q+KK++ ET+S YD EKL ER+AKL+GGVAV+KVGAATETE++D+KLR+EDA NAT AA+EEGIVPGGG  L HL+  +          +E +GA+IV  AL AP   IA NA          VK   +  GYNA    Y +ML
Sbjct:  320 RRVTINKDTTTIVAEG-NEVAVQARCEQIKKQMDETESTYDKEKLQERLAKLAGGVAVVKVGAATETEMKDKKLRLEDAINATKAAVEEGIVPGGGTTLAHLAPALEQWAASTLSGEELIGANIVAAALTAPLMRIAENAGVNGAVVAENVKAKSFNEGYNAANGDYVDML 489

HSP 2 Score: 30.8018 bits (68), Expect = 2.430e-43
Identity = 12/30 (40.00%), Postives = 20/30 (66.67%), Query Frame = 3 
Query:    3 KALLQDIAIVTGAEFQAGDLGLLIENTSVE 92
            KA+L+D+A++T  +    D GL +EN  +E
Sbjct:  285 KAMLEDMAVLTNGQLITEDAGLKLENAKIE 314
[Close]
BLAST of CK740108 vs. ExPASy Swiss-Prot
Match: CH60_SYNE7 (60 kDa chaperonin OS=Synechococcus elongatus (strain PCC 7942) GN=groL PE=3 SV=2)

HSP 1 Score: 163.31 bits (412), Expect = 2.430e-43
Identity = 85/170 (50.00%), Postives = 118/170 (69.41%), Query Frame = 2 
Query:  110 RKVTIRKDSTTIIADAASKDEIQARIAQLKKELAETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSDHVPAIKDKLEDADERLGADIVQKALVAPASLIAHNAXXXXXXXXXKVKDSEWTTGYNAMTDKYENM 619
            R++TI KD+TTI+A+  ++  ++AR+ Q+++++ ET+S YD EKL ER+AKLSGGVAV+KVGAATETE++DRKLR+EDA NAT AA+EEGIVPGGG  L HL+  +          +E  GA IV +AL AP   IA NA         +VK+  +  GY+A  +++ NM
Sbjct:  320 RRITITKDNTTIVAEG-NEAAVKARVDQIRRQIEETESSYDKEKLQERLAKLSGGVAVVKVGAATETEMKDRKLRLEDAINATKAAVEEGIVPGGGTTLAHLAPQLEEWATANLSGEELTGAQIVARALTAPLKRIAENAGLNGAVISERVKELPFDEGYDASNNQFVNM 488

HSP 2 Score: 32.7278 bits (73), Expect = 2.430e-43
Identity = 13/31 (41.94%), Postives = 22/31 (70.97%), Query Frame = 3 
Query:    3 KALLQDIAIVTGAEFQAGDLGLLIENTSVEQ 95
            KA+L+DIA++TG +    D GL ++ T ++Q
Sbjct:  285 KAMLEDIAVLTGGQLITEDAGLKLDTTKLDQ 315
[Close]
BLAST of CK740108 vs. ExPASy Swiss-Prot
Match: CH601_SYNP6 (60 kDa chaperonin 1 OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) GN=groL1 PE=3 SV=2)

HSP 1 Score: 163.31 bits (412), Expect = 2.430e-43
Identity = 85/170 (50.00%), Postives = 118/170 (69.41%), Query Frame = 2 
Query:  110 RKVTIRKDSTTIIADAASKDEIQARIAQLKKELAETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSDHVPAIKDKLEDADERLGADIVQKALVAPASLIAHNAXXXXXXXXXKVKDSEWTTGYNAMTDKYENM 619
            R++TI KD+TTI+A+  ++  ++AR+ Q+++++ ET+S YD EKL ER+AKLSGGVAV+KVGAATETE++DRKLR+EDA NAT AA+EEGIVPGGG  L HL+  +          +E  GA IV +AL AP   IA NA         +VK+  +  GY+A  +++ NM
Sbjct:  320 RRITITKDNTTIVAEG-NEAAVKARVDQIRRQIEETESSYDKEKLQERLAKLSGGVAVVKVGAATETEMKDRKLRLEDAINATKAAVEEGIVPGGGTTLAHLAPQLEEWATANLSGEELTGAQIVARALTAPLKRIAENAGLNGAVISERVKELPFDEGYDASNNQFVNM 488

HSP 2 Score: 32.7278 bits (73), Expect = 2.430e-43
Identity = 13/31 (41.94%), Postives = 22/31 (70.97%), Query Frame = 3 
Query:    3 KALLQDIAIVTGAEFQAGDLGLLIENTSVEQ 95
            KA+L+DIA++TG +    D GL ++ T ++Q
Sbjct:  285 KAMLEDIAVLTGGQLITEDAGLKLDTTKLDQ 315
[Close]
BLAST of CK740108 vs. ExPASy Swiss-Prot
Match: CH60_EUBR3 (60 kDa chaperonin OS=Eubacterium rectale (strain ATCC 33656 / VPI 0990) GN=groL PE=3 SV=1)

HSP 1 Score: 161.384 bits (407), Expect = 2.430e-43
Identity = 84/171 (49.12%), Postives = 119/171 (69.59%), Query Frame = 2 
Query:  110 RKVTIRKDSTTIIADAASKDEIQARIAQLKKELAETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSDHVPAIKDKLEDADERLGADIVQKALVAPASLIAHNAXXXXXXXXXKVKDSEWTTGYNAMTDKYENML 622
            + V ++K++T I+  A  KD I  R++Q++ ++ ET S +D EKL ER+AK++GGVAVI+VGAATETE+++ KLR+EDA NAT AA+EEGI+ GGG+A +H S  V  + D LE  DE+ GA ++ KAL AP   IA NA         KVK+S   TG+NA T++Y +M+
Sbjct:  319 KSVKVQKENTVIVDGAGDKDAIAGRVSQIRGQIDETTSEFDKEKLQERLAKMAGGVAVIRVGAATETEMKEAKLRMEDALNATRAAVEEGIIAGGGSAYIHASKKVAELVDTLE-GDEKTGAKVILKALEAPLYYIAANAGLEGAVIINKVKESAPGTGFNAATEEYVDMV 488

HSP 2 Score: 34.6538 bits (78), Expect = 2.430e-43
Identity = 14/30 (46.67%), Postives = 25/30 (83.33%), Query Frame = 3 
Query:    3 KALLQDIAIVTGAEFQAGDLGLLIENTSVE 92
            KA+L+DIAI+TG +  + DLGL +++T+++
Sbjct:  284 KAMLEDIAILTGGQVISSDLGLELKDTTID 313
[Close]
BLAST of CK740108 vs. ExPASy Swiss-Prot
Match: CH602_PROM5 (60 kDa chaperonin 2 OS=Prochlorococcus marinus (strain MIT 9515) GN=groL2 PE=3 SV=1)

HSP 1 Score: 165.236 bits (417), Expect = 3.167e-43
Identity = 87/170 (51.18%), Postives = 116/170 (68.24%), Query Frame = 2 
Query:  110 RKVTIRKDSTTIIADAASKDEIQARIAQLKKELAETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSDHVPAIKDKLEDADERLGADIVQKALVAPASLIAHNAXXXXXXXXXKVKDSEWTTGYNAMTDKYENM 619
            R++TI K++TTI+A+   K  + +R  Q+KK++ ETDS YD EKL ER+AKL+GGVAVIKVGAATETE++D+KLR+EDA NAT AA+EEGIVPGGG  L HL+  +    D     +E +GA+IV+ +L AP   IA NA          VK   +  G+NA T +Y +M
Sbjct:  320 RRITINKETTTIVAEGNEK-AVNSRCDQIKKQMEETDSSYDKEKLQERLAKLAGGVAVIKVGAATETEMKDKKLRLEDAINATKAAVEEGIVPGGGTTLAHLAPILKEWADATLSGEELIGANIVEASLTAPLMRIAENAGSNGAVIAENVKSKPFNDGFNAATGEYVDM 488

HSP 2 Score: 30.4166 bits (67), Expect = 3.167e-43
Identity = 12/30 (40.00%), Postives = 21/30 (70.00%), Query Frame = 3 
Query:    3 KALLQDIAIVTGAEFQAGDLGLLIENTSVE 92
            KA+L+D+A++T  +    D GL +EN ++E
Sbjct:  285 KAMLEDMAVLTNGQLITEDAGLKLENATLE 314
[Close]
BLAST of CK740108 vs. ExPASy Swiss-Prot
Match: CH602_PROM1 (60 kDa chaperonin 2 OS=Prochlorococcus marinus (strain NATL1A) GN=groL2 PE=3 SV=1)

HSP 1 Score: 166.392 bits (420), Expect = 3.167e-43
Identity = 92/174 (52.87%), Postives = 120/174 (68.97%), Query Frame = 2 
Query:  110 RKVTIRKDSTTIIADAASKDEIQARIAQLKKELAETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSDHVPAIKDKLE---DADERLGADIVQKALVAPASLIAHNAXXXXXXXXXKVKDSEWTTGYNAMTDKYENML 622
            R+VTI KD++TI+A+  ++  + AR  Q+KK++ ETDS YD EKL ER+AKLSGGVAV+KVGAATETE++D+KLR+EDA NAT AA+EEGIVPGGG  L HL+   PA++D        +E +GA+IV+ AL +P   IA NA          VK      GYNA T +Y +ML
Sbjct:  320 RRVTINKDTSTIVAEG-NEVAVNARCEQIKKQMDETDSTYDKEKLQERLAKLSGGVAVVKVGAATETEMKDKKLRLEDAINATKAAVEEGIVPGGGTTLAHLA---PALEDWSSTNLSGEELIGANIVEAALTSPLMRIAENAGANGAVVAENVKSKPVNDGYNAATGEYVDML 489

HSP 2 Score: 29.261 bits (64), Expect = 3.167e-43
Identity = 11/30 (36.67%), Postives = 21/30 (70.00%), Query Frame = 3 
Query:    3 KALLQDIAIVTGAEFQAGDLGLLIENTSVE 92
            KA+L+D+A++T  +    D GL +EN +++
Sbjct:  285 KAMLEDMAVLTNGQLITEDAGLKLENATLD 314
The following BLAST results are available for this feature:
BLAST of CK740108 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
CH601_ANAVT1.431e-4349.4160 kDa chaperonin 1 OS=Anabaena variabilis (strain... [more]
CH601_ANASP1.431e-4349.4160 kDa chaperonin 1 OS=Anabaena sp. (strain PCC 71... [more]
CH60_EMIHU1.432e-4351.1860 kDa chaperonin, chloroplastic OS=Emiliania huxl... [more]
CH602_PROMP1.865e-4351.7660 kDa chaperonin 2 OS=Prochlorococcus marinus sub... [more]
CH602_SYNPW2.430e-4352.0560 kDa chaperonin 2 OS=Synechococcus sp. (strain W... [more]
CH60_SYNE72.430e-4350.0060 kDa chaperonin OS=Synechococcus elongatus (stra... [more]
CH601_SYNP62.430e-4350.0060 kDa chaperonin 1 OS=Synechococcus sp. (strain A... [more]
CH60_EUBR32.430e-4349.1260 kDa chaperonin OS=Eubacterium rectale (strain A... [more]
CH602_PROM53.167e-4351.1860 kDa chaperonin 2 OS=Prochlorococcus marinus (st... [more]
CH602_PROM13.167e-4352.8760 kDa chaperonin 2 OS=Prochlorococcus marinus (st... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_6602 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE65E07 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK740108 ID=CK740108; Name=CK740108; organism=Citrus sinensis; type=EST; length=623bp
GAAAAGCTCTCCTTCAAGATATTGCCATTGTGACTGGAGCTGAGTTTCAA
GCTGGAGACCTGGGCTTGCTTATTGAAAACACTTCAGTTGAGCAGCCTAG
GTACAGCCTAGAAAGGTGACCATCAGAAAGGACTCCACTACCATTATTGC
TGATGCTGCATCAAAGGATGAAATACAAGCTAGAATTGCACAGCTGAAAA
AGGAGTTGGCTGAGACGGATTCTGTTTATGACTCAGAGAAACTTGCTGAG
AGAATTGCTAAATTGTCTGGCGGGGTTGCTGTTATTAAAGTTGGAGCTGC
CACAGAAACTGAGCTTGAGGACCGTAAACTCCGTATTGAAGATGCCAAGA
ATGCAACTTTTGCAGCCATAGAGGAAGGTATTGTTCCTGGTGGTGGTGCT
GCTCTGGTTCATCTGTCTGATCATGTTCCTGCTATCAAGGACAAGCTTGA
AGATGCAGATGAGCGATTAGGTGCTGACATTGTGCAGAAGGCACTGGTAG
CACCTGCATCACTGATAGCTCACAATGCTGGAGTTGAAGGTGAGGGTGTT
GTGGAGAAGGTGAAAGATAGTGAATGGACGACTGGGTACAATGCAATGAC
AGACAAGTATGAGAACATGCTTC
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