CK740108

Overview
NameCK740108
Unique NameCK740108
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length623
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK740108 vs. ExPASy Swiss-Prot
Match: CH60_EXIS2 (60 kDa chaperonin OS=Exiguobacterium sibiricum (strain DSM 17290 / JCM 13490 / 255-15) GN=groL PE=3 SV=1)

HSP 1 Score: 157.147 bits (396), Expect = 4.469e-42
Identity = 85/170 (50.00%), Postives = 112/170 (65.88%), Query Frame = 2 
Query:  113 KVTIRKDSTTIIADAASKDEIQARIAQLKKELAETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSDHVPAIKDKLEDADERLGADIVQKALVAPASLIAHNAXXXXXXXXXKVKDSEWTTGYNAMTDKYENML 622
            KV + KD+TTI+      D I AR+  ++ ++ ET S +D EKL ER+AKL+GGVAV+KVGAATETEL++RKLRIEDA NAT AA+EEGIV GGG A +H++  V +I   +   DE  G +IV +AL AP   IA NA         ++K      GYNA TD+Y +M+
Sbjct:  320 KVVVSKDNTTIVEGNGHSDSITARVGAIRSQIEETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKERKLRIEDALNATRAAVEEGIVAGGGTAFIHVTKAVESIL-AVTTGDEATGVNIVLRALEAPLRQIAENAGQEGSVIVERLKHEAQGMGYNAATDEYVDMI 488

HSP 2 Score: 34.6538 bits (78), Expect = 4.469e-42
Identity = 16/31 (51.61%), Postives = 23/31 (74.19%), Query Frame = 3 
Query:    3 KALLQDIAIVTGAEFQAGDLGLLIENTSVEQ 95
            KA+L+DI+IVTGAE    +LGL ++ T + Q
Sbjct:  284 KAMLEDISIVTGAELITEELGLDLKETQITQ 314
[Close]
BLAST of CK740108 vs. ExPASy Swiss-Prot
Match: CH60_LACAC (60 kDa chaperonin OS=Lactobacillus acidophilus GN=groL PE=3 SV=2)

HSP 1 Score: 162.54 bits (410), Expect = 4.469e-42
Identity = 93/174 (53.45%), Postives = 120/174 (68.97%), Query Frame = 2 
Query:  104 QPRKVTIRKDSTTIIADAASKDEIQARIAQLKKELAETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSDHVPAIKD-KLEDADERLGADIVQKALVAPASLIAHNAXXXXXXXXXKVKDSEWTTGYNAMTDKYENML 622
            Q R+VT+ KDSTTI+  A SKD I+ R   ++K++ E+ S +D +KL ER+AKL+GGVAVI VGAATETEL++R+ RIEDA N+T AA++EG V GGG ALV   D   AIKD K E +DE+ G +IV +AL AP   IA NA         K++  E   GYNA TDK+ENM+
Sbjct:  317 QARRVTVTKDSTTIVDGAGSKDAIKEREDSIRKQIEESTSDFDKKKLQERLAKLTGGVAVIHVGAATETELKERRYRIEDALNSTRAAVDEGYVAGGGTALV---DVEKAIKDLKGETSDEQTGINIVLRALSAPVRQIAENAGKDGAVVLNKLESQENEIGYNAATDKWENMV 487

HSP 2 Score: 29.261 bits (64), Expect = 4.469e-42
Identity = 13/31 (41.94%), Postives = 20/31 (64.52%), Query Frame = 3 
Query:    3 KALLQDIAIVTGAEFQAGDLGLLIENTSVEQ 95
            KA L+DIA +TG      DLG  +++T ++Q
Sbjct:  284 KAQLEDIAALTGGTVITDDLGFELKDTKIDQ 314
[Close]
BLAST of CK740108 vs. ExPASy Swiss-Prot
Match: CH60_LACH4 (60 kDa chaperonin OS=Lactobacillus helveticus (strain DPC 4571) GN=groL PE=3 SV=1)

HSP 1 Score: 160.229 bits (404), Expect = 4.470e-42
Identity = 86/173 (49.71%), Postives = 118/173 (68.21%), Query Frame = 2 
Query:  104 QPRKVTIRKDSTTIIADAASKDEIQARIAQLKKELAETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSDHVPAIKDKLEDADERLGADIVQKALVAPASLIAHNAXXXXXXXXXKVKDSEWTTGYNAMTDKYENML 622
            Q R++T+ KDSTTI+  A SK+ I  R+  ++K++ ++ S +D +KL ER+AKL+GGVAVI VGAATETEL++R+ RIEDA N+T AA++EG V GGG ALV++   V  +K   E  DE+ G +IV +AL AP   IA NA         K++  E   GYNA TDK+ENM+
Sbjct:  317 QARRITVTKDSTTIVDGAGSKEAIDERVDTIRKQIEDSTSDFDKKKLQERLAKLTGGVAVIHVGAATETELKERRYRIEDALNSTRAAVDEGYVAGGGTALVNVEKAVREVKG--ETTDEQTGINIVLRALSAPVRQIAENAGKDGSVILDKLEHQENEIGYNAATDKWENMV 487

HSP 2 Score: 31.5722 bits (70), Expect = 4.470e-42
Identity = 15/31 (48.39%), Postives = 21/31 (67.74%), Query Frame = 3 
Query:    3 KALLQDIAIVTGAEFQAGDLGLLIENTSVEQ 95
            KA LQDIA +TG      DLGL +++T ++Q
Sbjct:  284 KAQLQDIAALTGGTVITEDLGLELKDTKIDQ 314
[Close]
BLAST of CK740108 vs. ExPASy Swiss-Prot
Match: CH60_STAAW (60 kDa chaperonin OS=Staphylococcus aureus (strain MW2) GN=groL PE=3 SV=1)

HSP 1 Score: 158.303 bits (399), Expect = 4.470e-42
Identity = 86/170 (50.59%), Postives = 118/170 (69.41%), Query Frame = 2 
Query:  113 KVTIRKDSTTIIADAASKDEIQARIAQLKKELAETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSDHVPAIKDKLEDADERLGADIVQKALVAPASLIAHNAXXXXXXXXXKVKDSEWTTGYNAMTDKYENML 622
            KV + KD+TT++     ++ I AR++QLK ++ ET+S +D EKL ER+AKL+GGVAVIKVGAA+ETEL++RKLRIEDA N+T AA+EEGIV GGG ALV++   V  I+    + D   G +IV KAL AP   IA NA         ++K++E   G+NA T+++ NML
Sbjct:  320 KVEVTKDNTTVVDGDGDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKLRIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIE---AEGDIETGVNIVLKALTAPVRQIAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNML 486

HSP 2 Score: 33.4982 bits (75), Expect = 4.470e-42
Identity = 14/30 (46.67%), Postives = 24/30 (80.00%), Query Frame = 3 
Query:    3 KALLQDIAIVTGAEFQAGDLGLLIENTSVE 92
            KA+L+D+AI+TGA+    DLGL +++ S++
Sbjct:  284 KAMLEDLAILTGAQVITDDLGLDLKDASID 313
[Close]
BLAST of CK740108 vs. ExPASy Swiss-Prot
Match: CH60_STAAT (60 kDa chaperonin OS=Staphylococcus aureus (strain USA300 / TCH1516) GN=groL PE=3 SV=1)

HSP 1 Score: 158.303 bits (399), Expect = 4.470e-42
Identity = 86/170 (50.59%), Postives = 118/170 (69.41%), Query Frame = 2 
Query:  113 KVTIRKDSTTIIADAASKDEIQARIAQLKKELAETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSDHVPAIKDKLEDADERLGADIVQKALVAPASLIAHNAXXXXXXXXXKVKDSEWTTGYNAMTDKYENML 622
            KV + KD+TT++     ++ I AR++QLK ++ ET+S +D EKL ER+AKL+GGVAVIKVGAA+ETEL++RKLRIEDA N+T AA+EEGIV GGG ALV++   V  I+    + D   G +IV KAL AP   IA NA         ++K++E   G+NA T+++ NML
Sbjct:  320 KVEVTKDNTTVVDGDGDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKLRIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIE---AEGDIETGVNIVLKALTAPVRQIAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNML 486

HSP 2 Score: 33.4982 bits (75), Expect = 4.470e-42
Identity = 14/30 (46.67%), Postives = 24/30 (80.00%), Query Frame = 3 
Query:    3 KALLQDIAIVTGAEFQAGDLGLLIENTSVE 92
            KA+L+D+AI+TGA+    DLGL +++ S++
Sbjct:  284 KAMLEDLAILTGAQVITDDLGLDLKDASID 313
[Close]
BLAST of CK740108 vs. ExPASy Swiss-Prot
Match: CH60_STAAS (60 kDa chaperonin OS=Staphylococcus aureus (strain MSSA476) GN=groL PE=3 SV=1)

HSP 1 Score: 158.303 bits (399), Expect = 4.470e-42
Identity = 86/170 (50.59%), Postives = 118/170 (69.41%), Query Frame = 2 
Query:  113 KVTIRKDSTTIIADAASKDEIQARIAQLKKELAETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSDHVPAIKDKLEDADERLGADIVQKALVAPASLIAHNAXXXXXXXXXKVKDSEWTTGYNAMTDKYENML 622
            KV + KD+TT++     ++ I AR++QLK ++ ET+S +D EKL ER+AKL+GGVAVIKVGAA+ETEL++RKLRIEDA N+T AA+EEGIV GGG ALV++   V  I+    + D   G +IV KAL AP   IA NA         ++K++E   G+NA T+++ NML
Sbjct:  320 KVEVTKDNTTVVDGDGDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKLRIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIE---AEGDIETGVNIVLKALTAPVRQIAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNML 486

HSP 2 Score: 33.4982 bits (75), Expect = 4.470e-42
Identity = 14/30 (46.67%), Postives = 24/30 (80.00%), Query Frame = 3 
Query:    3 KALLQDIAIVTGAEFQAGDLGLLIENTSVE 92
            KA+L+D+AI+TGA+    DLGL +++ S++
Sbjct:  284 KAMLEDLAILTGAQVITDDLGLDLKDASID 313
[Close]
BLAST of CK740108 vs. ExPASy Swiss-Prot
Match: CH60_STAAN (60 kDa chaperonin OS=Staphylococcus aureus (strain N315) GN=groL PE=1 SV=1)

HSP 1 Score: 158.303 bits (399), Expect = 4.470e-42
Identity = 86/170 (50.59%), Postives = 118/170 (69.41%), Query Frame = 2 
Query:  113 KVTIRKDSTTIIADAASKDEIQARIAQLKKELAETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSDHVPAIKDKLEDADERLGADIVQKALVAPASLIAHNAXXXXXXXXXKVKDSEWTTGYNAMTDKYENML 622
            KV + KD+TT++     ++ I AR++QLK ++ ET+S +D EKL ER+AKL+GGVAVIKVGAA+ETEL++RKLRIEDA N+T AA+EEGIV GGG ALV++   V  I+    + D   G +IV KAL AP   IA NA         ++K++E   G+NA T+++ NML
Sbjct:  320 KVEVTKDNTTVVDGDGDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKLRIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIE---AEGDIETGVNIVLKALTAPVRQIAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNML 486

HSP 2 Score: 33.4982 bits (75), Expect = 4.470e-42
Identity = 14/30 (46.67%), Postives = 24/30 (80.00%), Query Frame = 3 
Query:    3 KALLQDIAIVTGAEFQAGDLGLLIENTSVE 92
            KA+L+D+AI+TGA+    DLGL +++ S++
Sbjct:  284 KAMLEDLAILTGAQVITDDLGLDLKDASID 313
[Close]
BLAST of CK740108 vs. ExPASy Swiss-Prot
Match: CH60_STAAM (60 kDa chaperonin OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) GN=groL PE=1 SV=1)

HSP 1 Score: 158.303 bits (399), Expect = 4.470e-42
Identity = 86/170 (50.59%), Postives = 118/170 (69.41%), Query Frame = 2 
Query:  113 KVTIRKDSTTIIADAASKDEIQARIAQLKKELAETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSDHVPAIKDKLEDADERLGADIVQKALVAPASLIAHNAXXXXXXXXXKVKDSEWTTGYNAMTDKYENML 622
            KV + KD+TT++     ++ I AR++QLK ++ ET+S +D EKL ER+AKL+GGVAVIKVGAA+ETEL++RKLRIEDA N+T AA+EEGIV GGG ALV++   V  I+    + D   G +IV KAL AP   IA NA         ++K++E   G+NA T+++ NML
Sbjct:  320 KVEVTKDNTTVVDGDGDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKLRIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIE---AEGDIETGVNIVLKALTAPVRQIAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNML 486

HSP 2 Score: 33.4982 bits (75), Expect = 4.470e-42
Identity = 14/30 (46.67%), Postives = 24/30 (80.00%), Query Frame = 3 
Query:    3 KALLQDIAIVTGAEFQAGDLGLLIENTSVE 92
            KA+L+D+AI+TGA+    DLGL +++ S++
Sbjct:  284 KAMLEDLAILTGAQVITDDLGLDLKDASID 313
[Close]
BLAST of CK740108 vs. ExPASy Swiss-Prot
Match: CH60_STAAE (60 kDa chaperonin OS=Staphylococcus aureus (strain Newman) GN=groL PE=3 SV=1)

HSP 1 Score: 158.303 bits (399), Expect = 4.470e-42
Identity = 86/170 (50.59%), Postives = 118/170 (69.41%), Query Frame = 2 
Query:  113 KVTIRKDSTTIIADAASKDEIQARIAQLKKELAETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSDHVPAIKDKLEDADERLGADIVQKALVAPASLIAHNAXXXXXXXXXKVKDSEWTTGYNAMTDKYENML 622
            KV + KD+TT++     ++ I AR++QLK ++ ET+S +D EKL ER+AKL+GGVAVIKVGAA+ETEL++RKLRIEDA N+T AA+EEGIV GGG ALV++   V  I+    + D   G +IV KAL AP   IA NA         ++K++E   G+NA T+++ NML
Sbjct:  320 KVEVTKDNTTVVDGDGDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKLRIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIE---AEGDIETGVNIVLKALTAPVRQIAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNML 486

HSP 2 Score: 33.4982 bits (75), Expect = 4.470e-42
Identity = 14/30 (46.67%), Postives = 24/30 (80.00%), Query Frame = 3 
Query:    3 KALLQDIAIVTGAEFQAGDLGLLIENTSVE 92
            KA+L+D+AI+TGA+    DLGL +++ S++
Sbjct:  284 KAMLEDLAILTGAQVITDDLGLDLKDASID 313
[Close]
BLAST of CK740108 vs. ExPASy Swiss-Prot
Match: CH60_STAAC (60 kDa chaperonin OS=Staphylococcus aureus (strain COL) GN=groL PE=3 SV=1)

HSP 1 Score: 158.303 bits (399), Expect = 4.470e-42
Identity = 86/170 (50.59%), Postives = 118/170 (69.41%), Query Frame = 2 
Query:  113 KVTIRKDSTTIIADAASKDEIQARIAQLKKELAETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSDHVPAIKDKLEDADERLGADIVQKALVAPASLIAHNAXXXXXXXXXKVKDSEWTTGYNAMTDKYENML 622
            KV + KD+TT++     ++ I AR++QLK ++ ET+S +D EKL ER+AKL+GGVAVIKVGAA+ETEL++RKLRIEDA N+T AA+EEGIV GGG ALV++   V  I+    + D   G +IV KAL AP   IA NA         ++K++E   G+NA T+++ NML
Sbjct:  320 KVEVTKDNTTVVDGDGDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKLRIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIE---AEGDIETGVNIVLKALTAPVRQIAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNML 486

HSP 2 Score: 33.4982 bits (75), Expect = 4.470e-42
Identity = 14/30 (46.67%), Postives = 24/30 (80.00%), Query Frame = 3 
Query:    3 KALLQDIAIVTGAEFQAGDLGLLIENTSVE 92
            KA+L+D+AI+TGA+    DLGL +++ S++
Sbjct:  284 KAMLEDLAILTGAQVITDDLGLDLKDASID 313
The following BLAST results are available for this feature:
BLAST of CK740108 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
CH60_EXIS24.469e-4250.0060 kDa chaperonin OS=Exiguobacterium sibiricum (st... [more]
CH60_LACAC4.469e-4253.4560 kDa chaperonin OS=Lactobacillus acidophilus GN=... [more]
CH60_LACH44.470e-4249.7160 kDa chaperonin OS=Lactobacillus helveticus (str... [more]
CH60_STAAW4.470e-4250.5960 kDa chaperonin OS=Staphylococcus aureus (strain... [more]
CH60_STAAT4.470e-4250.5960 kDa chaperonin OS=Staphylococcus aureus (strain... [more]
CH60_STAAS4.470e-4250.5960 kDa chaperonin OS=Staphylococcus aureus (strain... [more]
CH60_STAAN4.470e-4250.5960 kDa chaperonin OS=Staphylococcus aureus (strain... [more]
CH60_STAAM4.470e-4250.5960 kDa chaperonin OS=Staphylococcus aureus (strain... [more]
CH60_STAAE4.470e-4250.5960 kDa chaperonin OS=Staphylococcus aureus (strain... [more]
CH60_STAAC4.470e-4250.5960 kDa chaperonin OS=Staphylococcus aureus (strain... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_6602 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE65E07 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK740108 ID=CK740108; Name=CK740108; organism=Citrus sinensis; type=EST; length=623bp
GAAAAGCTCTCCTTCAAGATATTGCCATTGTGACTGGAGCTGAGTTTCAA
GCTGGAGACCTGGGCTTGCTTATTGAAAACACTTCAGTTGAGCAGCCTAG
GTACAGCCTAGAAAGGTGACCATCAGAAAGGACTCCACTACCATTATTGC
TGATGCTGCATCAAAGGATGAAATACAAGCTAGAATTGCACAGCTGAAAA
AGGAGTTGGCTGAGACGGATTCTGTTTATGACTCAGAGAAACTTGCTGAG
AGAATTGCTAAATTGTCTGGCGGGGTTGCTGTTATTAAAGTTGGAGCTGC
CACAGAAACTGAGCTTGAGGACCGTAAACTCCGTATTGAAGATGCCAAGA
ATGCAACTTTTGCAGCCATAGAGGAAGGTATTGTTCCTGGTGGTGGTGCT
GCTCTGGTTCATCTGTCTGATCATGTTCCTGCTATCAAGGACAAGCTTGA
AGATGCAGATGAGCGATTAGGTGCTGACATTGTGCAGAAGGCACTGGTAG
CACCTGCATCACTGATAGCTCACAATGCTGGAGTTGAAGGTGAGGGTGTT
GTGGAGAAGGTGAAAGATAGTGAATGGACGACTGGGTACAATGCAATGAC
AGACAAGTATGAGAACATGCTTC
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