CK740108 | Citrus Genome Database

Overview


Name	CK740108
Unique Name	CK740108
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	623

Libraries


Library Name	Type
Ridge pineapple sweet orange entire seedling	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of CK740108 vs. ExPASy Swiss-Prot
Match: CH60_STAA9 (60 kDa chaperonin OS=Staphylococcus aureus (strain JH9) GN=groL PE=3 SV=1)

HSP 1 Score: 158.303 bits (399), Expect = 4.470e-42
Identity = 86/170 (50.59%), Postives = 118/170 (69.41%), Query Frame = 2

Query:  113 KVTIRKDSTTIIADAASKDEIQARIAQLKKELAETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSDHVPAIKDKLEDADERLGADIVQKALVAPASLIAHNAXXXXXXXXXKVKDSEWTTGYNAMTDKYENML 622
            KV + KD+TT++     ++ I AR++QLK ++ ET+S +D EKL ER+AKL+GGVAVIKVGAA+ETEL++RKLRIEDA N+T AA+EEGIV GGG ALV++   V  I+    + D   G +IV KAL AP   IA NA         ++K++E   G+NA T+++ NML
Sbjct:  320 KVEVTKDNTTVVDGDGDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKLRIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIE---AEGDIETGVNIVLKALTAPVRQIAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNML 486

HSP 2 Score: 33.4982 bits (75), Expect = 4.470e-42
Identity = 14/30 (46.67%), Postives = 24/30 (80.00%), Query Frame = 3

Query:    3 KALLQDIAIVTGAEFQAGDLGLLIENTSVE 92
            KA+L+D+AI+TGA+    DLGL +++ S++
Sbjct:  284 KAMLEDLAILTGAQVITDDLGLDLKDASID 313

BLAST of CK740108 vs. ExPASy Swiss-Prot
Match: CH60_STAA8 (60 kDa chaperonin OS=Staphylococcus aureus (strain NCTC 8325) GN=groL PE=3 SV=1)

HSP 1 Score: 158.303 bits (399), Expect = 4.470e-42
Identity = 86/170 (50.59%), Postives = 118/170 (69.41%), Query Frame = 2

Query:  113 KVTIRKDSTTIIADAASKDEIQARIAQLKKELAETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSDHVPAIKDKLEDADERLGADIVQKALVAPASLIAHNAXXXXXXXXXKVKDSEWTTGYNAMTDKYENML 622
            KV + KD+TT++     ++ I AR++QLK ++ ET+S +D EKL ER+AKL+GGVAVIKVGAA+ETEL++RKLRIEDA N+T AA+EEGIV GGG ALV++   V  I+    + D   G +IV KAL AP   IA NA         ++K++E   G+NA T+++ NML
Sbjct:  320 KVEVTKDNTTVVDGDGDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKLRIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIE---AEGDIETGVNIVLKALTAPVRQIAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNML 486

HSP 2 Score: 33.4982 bits (75), Expect = 4.470e-42
Identity = 14/30 (46.67%), Postives = 24/30 (80.00%), Query Frame = 3

Query:    3 KALLQDIAIVTGAEFQAGDLGLLIENTSVE 92
            KA+L+D+AI+TGA+    DLGL +++ S++
Sbjct:  284 KAMLEDLAILTGAQVITDDLGLDLKDASID 313

BLAST of CK740108 vs. ExPASy Swiss-Prot
Match: CH60_STAA3 (60 kDa chaperonin OS=Staphylococcus aureus (strain USA300) GN=groL PE=3 SV=1)

HSP 1 Score: 158.303 bits (399), Expect = 4.470e-42
Identity = 86/170 (50.59%), Postives = 118/170 (69.41%), Query Frame = 2

Query:  113 KVTIRKDSTTIIADAASKDEIQARIAQLKKELAETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSDHVPAIKDKLEDADERLGADIVQKALVAPASLIAHNAXXXXXXXXXKVKDSEWTTGYNAMTDKYENML 622
            KV + KD+TT++     ++ I AR++QLK ++ ET+S +D EKL ER+AKL+GGVAVIKVGAA+ETEL++RKLRIEDA N+T AA+EEGIV GGG ALV++   V  I+    + D   G +IV KAL AP   IA NA         ++K++E   G+NA T+++ NML
Sbjct:  320 KVEVTKDNTTVVDGDGDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKLRIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIE---AEGDIETGVNIVLKALTAPVRQIAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNML 486

HSP 2 Score: 33.4982 bits (75), Expect = 4.470e-42
Identity = 14/30 (46.67%), Postives = 24/30 (80.00%), Query Frame = 3

Query:    3 KALLQDIAIVTGAEFQAGDLGLLIENTSVE 92
            KA+L+D+AI+TGA+    DLGL +++ S++
Sbjct:  284 KAMLEDLAILTGAQVITDDLGLDLKDASID 313

BLAST of CK740108 vs. ExPASy Swiss-Prot
Match: CH60_STAA2 (60 kDa chaperonin OS=Staphylococcus aureus (strain JH1) GN=groL PE=3 SV=1)

HSP 1 Score: 158.303 bits (399), Expect = 4.470e-42
Identity = 86/170 (50.59%), Postives = 118/170 (69.41%), Query Frame = 2

Query:  113 KVTIRKDSTTIIADAASKDEIQARIAQLKKELAETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSDHVPAIKDKLEDADERLGADIVQKALVAPASLIAHNAXXXXXXXXXKVKDSEWTTGYNAMTDKYENML 622
            KV + KD+TT++     ++ I AR++QLK ++ ET+S +D EKL ER+AKL+GGVAVIKVGAA+ETEL++RKLRIEDA N+T AA+EEGIV GGG ALV++   V  I+    + D   G +IV KAL AP   IA NA         ++K++E   G+NA T+++ NML
Sbjct:  320 KVEVTKDNTTVVDGDGDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKLRIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIE---AEGDIETGVNIVLKALTAPVRQIAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNML 486

HSP 2 Score: 33.4982 bits (75), Expect = 4.470e-42
Identity = 14/30 (46.67%), Postives = 24/30 (80.00%), Query Frame = 3

Query:    3 KALLQDIAIVTGAEFQAGDLGLLIENTSVE 92
            KA+L+D+AI+TGA+    DLGL +++ S++
Sbjct:  284 KAMLEDLAILTGAQVITDDLGLDLKDASID 313

BLAST of CK740108 vs. ExPASy Swiss-Prot
Match: CH60_STAA1 (60 kDa chaperonin OS=Staphylococcus aureus (strain Mu3 / ATCC 700698) GN=groL PE=3 SV=1)

HSP 1 Score: 158.303 bits (399), Expect = 4.470e-42
Identity = 86/170 (50.59%), Postives = 118/170 (69.41%), Query Frame = 2

Query:  113 KVTIRKDSTTIIADAASKDEIQARIAQLKKELAETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSDHVPAIKDKLEDADERLGADIVQKALVAPASLIAHNAXXXXXXXXXKVKDSEWTTGYNAMTDKYENML 622
            KV + KD+TT++     ++ I AR++QLK ++ ET+S +D EKL ER+AKL+GGVAVIKVGAA+ETEL++RKLRIEDA N+T AA+EEGIV GGG ALV++   V  I+    + D   G +IV KAL AP   IA NA         ++K++E   G+NA T+++ NML
Sbjct:  320 KVEVTKDNTTVVDGDGDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKLRIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIE---AEGDIETGVNIVLKALTAPVRQIAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNML 486

HSP 2 Score: 33.4982 bits (75), Expect = 4.470e-42
Identity = 14/30 (46.67%), Postives = 24/30 (80.00%), Query Frame = 3

Query:    3 KALLQDIAIVTGAEFQAGDLGLLIENTSVE 92
            KA+L+D+AI+TGA+    DLGL +++ S++
Sbjct:  284 KAMLEDLAILTGAQVITDDLGLDLKDASID 313

BLAST of CK740108 vs. ExPASy Swiss-Prot
Match: CH602_SYNSC (60 kDa chaperonin 2 OS=Synechococcus sp. (strain CC9605) GN=groL2 PE=3 SV=1)

HSP 1 Score: 161.384 bits (407), Expect = 5.823e-42
Identity = 87/171 (50.88%), Postives = 115/171 (67.25%), Query Frame = 2

Query:  110 RKVTIRKDSTTIIADAASKDEIQARIAQLKKELAETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSDHVPAIKDKLEDADERLGADIVQKALVAPASLIAHNAXXXXXXXXXKVKDSEWTTGYNAMTDKYENML 622
            R++TI KD+TTI+A+  ++  + AR  Q+KK++ ETDS YD EKL ER+AKL+GGVAV+KVGAATETE++D+KLR+EDA NAT AA+EEGIVPGGG  L HL+  +          +E +GA+IV  AL AP   IA NA          VK      G+NA + +Y +ML
Sbjct:  320 RRITINKDTTTIVAEG-NEAAVGARCEQIKKQMDETDSTYDKEKLQERLAKLAGGVAVVKVGAATETEMKDKKLRLEDAINATKAAVEEGIVPGGGTTLAHLAPALEQWAASSLSGEELIGANIVAAALTAPLMRIAENAGANGAVVAENVKARAGAEGFNAASGEYVDML 489

HSP 2 Score: 30.0314 bits (66), Expect = 5.823e-42
Identity = 12/30 (40.00%), Postives = 20/30 (66.67%), Query Frame = 3

Query:    3 KALLQDIAIVTGAEFQAGDLGLLIENTSVE 92
            KA+L+D+A++T  +    D GL +EN  +E
Sbjct:  285 KAMLEDMAVLTNGQLITEDAGLKLENAKLE 314

BLAST of CK740108 vs. ExPASy Swiss-Prot
Match: CH60_ALKOO (60 kDa chaperonin OS=Alkaliphilus oremlandii (strain OhILAs) GN=groL PE=3 SV=1)

HSP 1 Score: 164.081 bits (414), Expect = 5.824e-42
Identity = 90/173 (52.02%), Postives = 120/173 (69.36%), Query Frame = 2

Query:  110 RKVTIRKDSTTIIADAASKDEIQARIAQLKKELAETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSDHVPAIKDKLE--DADERLGADIVQKALVAPASLIAHNAXXXXXXXXXKVKDSEWTTGYNAMTDKYENML 622
            R V + KD+TTI+  A     I+ R+ Q+K+++ ET S +D EKL ER+AKLSGGVAVI+VGAATETEL++RKLRIEDA NAT A +EEG+V GGGA+LVH+   +PA++  LE  + DER G  I+++AL  P   IA NA         KV  SE   G++A+T+KY NM+
Sbjct:  319 RTVKVDKDNTTIVEGAGDSSAIKDRVNQIKRQIEETTSDFDKEKLQERLAKLSGGVAVIQVGAATETELKERKLRIEDALNATRAGVEEGMVAGGGASLVHV---IPAVEALLETTEGDERTGVKIIRRALEEPLRQIAANAGLEGSVIVEKVMSSEKGIGFDALTEKYVNMI 488

HSP 2 Score: 27.335 bits (59), Expect = 5.824e-42
Identity = 11/30 (36.67%), Postives = 21/30 (70.00%), Query Frame = 3

Query:    3 KALLQDIAIVTGAEFQAGDLGLLIENTSVE 92
            KA+L DIA++TGA   + +LG  ++  +++
Sbjct:  284 KAMLDDIAVLTGATVISDELGYDLKTATID 313

BLAST of CK740108 vs. ExPASy Swiss-Prot
Match: CH60_LACPL (60 kDa chaperonin OS=Lactobacillus plantarum GN=groL PE=3 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 5.824e-42
Identity = 85/173 (49.13%), Postives = 117/173 (67.63%), Query Frame = 2

Query:  104 QPRKVTIRKDSTTIIADAASKDEIQARIAQLKKELAETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSDHVPAIKDKLEDADERLGADIVQKALVAPASLIAHNAXXXXXXXXXKVKDSEWTTGYNAMTDKYENML 622
            Q  KVT+ KD+TTI+  A SKD I  R+  ++ ++ ET S +D EKL ER+AKL+GGVAV++VGAATETEL++RK RIEDA NAT AA+EEG V GGG AL+++   V A+K   E  D + G +IV++AL  P   IA NA         K+K+ +   G+NA TD++ +M+
Sbjct:  317 QANKVTVTKDNTTIVEGAGSKDAISERVEFIRNQIGETTSDFDKEKLQERLAKLAGGVAVVRVGAATETELKERKYRIEDALNATRAAVEEGFVAGGGTALINVIKDVAALK---ETGDVQTGINIVKRALEEPVRQIAENAGLEGSVIVEKMKEQKPGVGFNAATDEWVDMI 486

HSP 2 Score: 32.3426 bits (72), Expect = 5.824e-42
Identity = 15/31 (48.39%), Postives = 22/31 (70.97%), Query Frame = 3

Query:    3 KALLQDIAIVTGAEFQAGDLGLLIENTSVEQ 95
            K  LQDIAI+TG      DLGL +++T+++Q
Sbjct:  284 KEQLQDIAILTGGTVITDDLGLELKDTTIDQ 314

BLAST of CK740108 vs. ExPASy Swiss-Prot
Match: CH60_CLOTH (60 kDa chaperonin OS=Clostridium thermocellum (strain ATCC 27405 / DSM 1237) GN=groL PE=1 SV=3)

HSP 1 Score: 153.68 bits (387), Expect = 5.824e-42
Identity = 85/171 (49.71%), Postives = 114/171 (66.67%), Query Frame = 2

Query:  110 RKVTIRKDSTTIIADAASKDEIQARIAQLKKELAETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSDHVPAIKDKLEDADERLGADIVQKALVAPASLIAHNAXXXXXXXXXKVKDSEWTTGYNAMTDKYENML 622
            R+V ++K++T I+  A    EIQ RIA +K ++ ET S +D EKL ER+AKL+GGVAVI+VGAATETE++++KLRIEDA  AT AA+EEGIV GGG ALV++   V  + D +   DE+ G  I+ +AL  P   IA NA         KVK SE   G++A  +KY NM+
Sbjct:  319 RQVKVQKENTIIVDGAGDPKEIQKRIASIKSQIEETTSDFDREKLQERLAKLAGGVAVIQVGAATETEMKEKKLRIEDALAATKAAVEEGIVAGGGTALVNVIPKVAKVLDTV-SGDEKTGVQIILRALEEPVRQIAENAGLEGSVIVEKVKASEPGIGFDAYNEKYVNMI 488

HSP 2 Score: 37.7354 bits (86), Expect = 5.824e-42
Identity = 17/31 (54.84%), Postives = 25/31 (80.65%), Query Frame = 3

Query:    3 KALLQDIAIVTGAEFQAGDLGLLIENTSVEQ 95
            KA+L+DIAI+TG +    DLGL +++T+VEQ
Sbjct:  284 KAMLEDIAILTGGQVITSDLGLELKDTTVEQ 314

BLAST of CK740108 vs. ExPASy Swiss-Prot
Match: RUBB_PEA (RuBisCO large subunit-binding protein subunit beta, chloroplastic OS=Pisum sativum PE=1 SV=2)

HSP 1 Score: 169.088 bits (427), Expect = 7.581e-42
Identity = 88/171 (51.46%), Postives = 122/171 (71.35%), Query Frame = 2

Query:  113 KVTIRKDSTTIIADAASKDEIQARIAQLKKELAETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSDHVPAIKDKLEDADERLGADIVQKALVAPASLIAHNAXXXXXXXXXKVKDSEWTT-GYNAMTDKYENML 622
            KV + KD+TTI+ D ++++ +  R++Q+K ++   +  Y+ EKL+ERIAKLSGGVAVI+VGA TETEL+++KLR+EDA NAT AA+EEGIV GGG  L+ L+  V AIKD L + +E++GADIV++AL  P  LIA NA         KV  S+    GYNA T KYE+++
Sbjct:  370 KVVLTKDTTTIVGDGSTQEAVNKRVSQIKNQIEAAEQEYEKEKLSERIAKLSGGVAVIQVGAQTETELKEKKLRVEDALNATKAAVEEGIVVGGGCTLLRLASKVDAIKDTLANDEEKVGADIVKRALSYPLKLIAKNAGVNGSVVSEKVLSSDNPKYGYNAATGKYEDLM 540

HSP 2 Score: 21.9422 bits (45), Expect = 7.581e-42
Identity = 10/27 (37.04%), Postives = 15/27 (55.56%), Query Frame = 3

Query:   12 LQDIAIVTGAEFQAGDLGLLIENTSVE 92
            L DIAI+TG      ++GL ++    E
Sbjct:  337 LDDIAILTGGTVIREEVGLTLDKADKE 363

The following BLAST results are available for this feature:

BLAST of CK740108 vs. ExPASy Swiss-Prot

BLAST of CK740108 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
CH60_STAA9	4.470e-42	50.59	60 kDa chaperonin OS=Staphylococcus aureus (strain...	[more]
CH60_STAA8	4.470e-42	50.59	60 kDa chaperonin OS=Staphylococcus aureus (strain...	[more]
CH60_STAA3	4.470e-42	50.59	60 kDa chaperonin OS=Staphylococcus aureus (strain...	[more]
CH60_STAA2	4.470e-42	50.59	60 kDa chaperonin OS=Staphylococcus aureus (strain...	[more]
CH60_STAA1	4.470e-42	50.59	60 kDa chaperonin OS=Staphylococcus aureus (strain...	[more]
CH602_SYNSC	5.823e-42	50.88	60 kDa chaperonin 2 OS=Synechococcus sp. (strain C...	[more]
CH60_ALKOO	5.824e-42	52.02	60 kDa chaperonin OS=Alkaliphilus oremlandii (stra...	[more]
CH60_LACPL	5.824e-42	49.13	60 kDa chaperonin OS=Lactobacillus plantarum GN=gr...	[more]
CH60_CLOTH	5.824e-42	49.71	60 kDa chaperonin OS=Clostridium thermocellum (str...	[more]
RUBB_PEA	7.581e-42	51.46	RuBisCO large subunit-binding protein subunit beta...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_6602 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE65E07 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK740108 ID=CK740108; Name=CK740108; organism=Citrus sinensis; type=EST; length=623bp
GAAAAGCTCTCCTTCAAGATATTGCCATTGTGACTGGAGCTGAGTTTCAA
GCTGGAGACCTGGGCTTGCTTATTGAAAACACTTCAGTTGAGCAGCCTAG
GTACAGCCTAGAAAGGTGACCATCAGAAAGGACTCCACTACCATTATTGC
TGATGCTGCATCAAAGGATGAAATACAAGCTAGAATTGCACAGCTGAAAA
AGGAGTTGGCTGAGACGGATTCTGTTTATGACTCAGAGAAACTTGCTGAG
AGAATTGCTAAATTGTCTGGCGGGGTTGCTGTTATTAAAGTTGGAGCTGC
CACAGAAACTGAGCTTGAGGACCGTAAACTCCGTATTGAAGATGCCAAGA
ATGCAACTTTTGCAGCCATAGAGGAAGGTATTGTTCCTGGTGGTGGTGCT
GCTCTGGTTCATCTGTCTGATCATGTTCCTGCTATCAAGGACAAGCTTGA
AGATGCAGATGAGCGATTAGGTGCTGACATTGTGCAGAAGGCACTGGTAG
CACCTGCATCACTGATAGCTCACAATGCTGGAGTTGAAGGTGAGGGTGTT
GTGGAGAAGGTGAAAGATAGTGAATGGACGACTGGGTACAATGCAATGAC
AGACAAGTATGAGAACATGCTTC