CK740263

Overview
NameCK740263
Unique NameCK740263
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length721
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig4526 contig Csv1_Contig4526:126..850. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CK740263 vs. ExPASy Swiss-Prot
Match: TPIC_SECCE (Triosephosphate isomerase, chloroplastic OS=Secale cereale PE=1 SV=1)

HSP 1 Score: 228.794 bits (582), Expect = 1.631e-64
Identity = 108/171 (63.16%), Postives = 132/171 (77.19%), Query Frame = 3 
Query:   81 FLFLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLGIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPAQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGA 593
            F+++  VKSSL     V+AQN W+ KGGAFTGEISAE LV++G  WVILGHSERR ++ E +EF+G K AYALSQ LKV+AC+GE LE+REAG T DV   Q KA AD ++ W+N+V+AYEPVWAIGTGKVA+P QAQEVH  +R WL  N S ++A+  RIIYGGSVN A
Sbjct:   88 FIYIDQVKSSLTDRIEVSAQNTWIGKGGAFTGEISAEQLVDIGCQWVILGHSERRHVIGEDDEFIGKKAAYALSQNLKVMACIGELLEEREAGKTFDVCFKQMKAFADNITDWTNVVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKTNVSADVASTVRIIYGGSVNAA 258

HSP 2 Score: 37.3502 bits (85), Expect = 1.631e-64
Identity = 16/21 (76.19%), Postives = 18/21 (85.71%), Query Frame = 2 
Query:  593 NCKELAAQPDVDGFLVGGASL 655
            NC ELA + D+DGFLVGGASL
Sbjct:  259 NCAELAKKEDIDGFLVGGASL 279
[Close]
BLAST of CK740263 vs. ExPASy Swiss-Prot
Match: TPIC_ARATH (Triosephosphate isomerase, chloroplastic OS=Arabidopsis thaliana GN=TIM PE=1 SV=1)

HSP 1 Score: 228.794 bits (582), Expect = 1.777e-63
Identity = 112/173 (64.74%), Postives = 131/173 (75.72%), Query Frame = 3 
Query:   81 FLFLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLGIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPAQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGATA 599
            F+++  VKSSL     ++ QN WV KGGAFTGEIS E L +LG  WVILGHSERR ++ E +EF+G K AYALS+GL VIAC+GE LE+REAG T DV  AQ KA AD V SW NIV+AYEPVWAIGTGKVA+P QAQEVH  +R WL  N S E+A+ TRIIYGGSVNG  +
Sbjct:  105 FVYIDQVKSSLTDRIDISGQNSWVGKGGAFTGEISVEQLKDLGCKWVILGHSERRHVIGEKDEFIGKKAAYALSEGLGVIACIGEKLEEREAGKTFDVCFAQLKAFADAVPSWDNIVVAYEPVWAIGTGKVASPQQAQEVHVAVRGWLKKNVSEEVASKTRIIYGGSVNGGNS 277

HSP 2 Score: 33.8834 bits (76), Expect = 1.777e-63
Identity = 15/21 (71.43%), Postives = 17/21 (80.95%), Query Frame = 2 
Query:  593 NCKELAAQPDVDGFLVGGASL 655
            N  ELA + D+DGFLVGGASL
Sbjct:  276 NSAELAKEEDIDGFLVGGASL 296
[Close]
BLAST of CK740263 vs. ExPASy Swiss-Prot
Match: TPIC_FRAAN (Triosephosphate isomerase, chloroplastic OS=Fragaria ananassa GN=TPI PE=2 SV=1)

HSP 1 Score: 227.254 bits (578), Expect = 5.137e-63
Identity = 115/199 (57.79%), Postives = 139/199 (69.85%), Query Frame = 3 
Query:    3 VKKIVSVLNEGQXXXXXXXXXXXXXXFLFLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLGIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPAQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGATA 599
            + K+VS LN  +              FL+L  VKSSL     ++ QN WV KGGAFTGEIS E L ++G  WVILGHSERR ++ E ++F+G K AYAL++GL VIAC+GE LE+REAG T DV   Q KA AD V SW NIV+AYEPVWAIGTGKVA+P QAQEVH  +R+WL  N S E+A+ TRIIYGGSVNG  +
Sbjct:   80 ISKLVSDLNSAKLEPDVDVVVAPP--FLYLDQVKSSLTDRIEISGQNSWVAKGGAFTGEISVEQLKDIGRKWVILGHSERRHVIGEDDQFIGKKAAYALNEGLGVIACIGEKLEEREAGKTFDVCYEQLKAFADAVPSWENIVVAYEPVWAIGTGKVASPQQAQEVHVAVREWLKKNVSAEVASKTRIIYGGSVNGGNS 276

HSP 2 Score: 33.8834 bits (76), Expect = 5.137e-63
Identity = 15/21 (71.43%), Postives = 17/21 (80.95%), Query Frame = 2 
Query:  593 NCKELAAQPDVDGFLVGGASL 655
            N  ELA + D+DGFLVGGASL
Sbjct:  275 NSAELAKEEDIDGFLVGGASL 295
[Close]
BLAST of CK740263 vs. ExPASy Swiss-Prot
Match: TPIS_CULTA (Triosephosphate isomerase OS=Culex tarsalis GN=Tpi PE=1 SV=2)

HSP 1 Score: 214.927 bits (546), Expect = 3.300e-62
Identity = 108/172 (62.79%), Postives = 127/172 (73.84%), Query Frame = 3 
Query:   87 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLGIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPAQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGATAR 602
            +L L +S L     VAAQNC+    GAFTGEIS  ML +L I WVILGHSERR I  ES+E + DKVA+AL++GLKVIAC+GETL++REAG T  V   QTKAIAD+V  WSN+V+AYEPVWAIGTGK A+P QAQEVH  LRKW   N S +++AA RI YGGSV  A  R
Sbjct:   46 YLTLARSQLPDSVCVAAQNCYKVPKGAFTGEISPAMLKDLNIGWVILGHSERRAIFGESDELIADKVAHALAEGLKVIACIGETLQEREAGQTEAVCFRQTKAIADKVKDWSNVVIAYEPVWAIGTGKTASPEQAQEVHAALRKWFTENVSADVSAAIRIQYGGSVTAANCR 217

HSP 2 Score: 43.5134 bits (101), Expect = 3.300e-62
Identity = 18/21 (85.71%), Postives = 21/21 (100.00%), Query Frame = 2 
Query:  593 NCKELAAQPDVDGFLVGGASL 655
            NC+ELAA+PD+DGFLVGGASL
Sbjct:  215 NCRELAAKPDIDGFLVGGASL 235
[Close]
BLAST of CK740263 vs. ExPASy Swiss-Prot
Match: TPIS_RABIT (Triosephosphate isomerase OS=Oryctolagus cuniculus GN=TPI1 PE=1 SV=1)

HSP 1 Score: 211.846 bits (538), Expect = 3.595e-61
Identity = 104/172 (60.47%), Postives = 125/172 (72.67%), Query Frame = 3 
Query:   87 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLGIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPAQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGATAR 602
            ++   +  L P   VAAQNC+    GAFTGEIS  M+ + G  WV+LGHSERR +  ES+E +G KVA+ALS+GL VIAC+GE L++REAG T  VV  QTK IAD V  WS +VLAYEPVWAIGTGK ATP QAQEVH +LR WL +N S  +A +TRIIYGGSV GAT +
Sbjct:   47 YIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALSEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCK 218

HSP 2 Score: 43.1282 bits (100), Expect = 3.595e-61
Identity = 19/20 (95.00%), Postives = 20/20 (100.00%), Query Frame = 2 
Query:  596 CKELAAQPDVDGFLVGGASL 655
            CKELA+QPDVDGFLVGGASL
Sbjct:  217 CKELASQPDVDGFLVGGASL 236
[Close]
BLAST of CK740263 vs. ExPASy Swiss-Prot
Match: TPIS_PONAB (Triosephosphate isomerase OS=Pongo abelii GN=TPI1 PE=2 SV=1)

HSP 1 Score: 210.69 bits (535), Expect = 7.967e-61
Identity = 103/172 (59.88%), Postives = 125/172 (72.67%), Query Frame = 3 
Query:   87 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLGIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPAQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGATAR 602
            ++   +  L P   VAAQNC+    GAFTGEIS  M+ + G  WV+LGHSERR +  ES+E +G KVA+AL++GL VIAC+GE L++REAG T  VV  QTK IAD V  WS +VLAYEPVWAIGTGK ATP QAQEVH +LR WL +N S  +A +TRIIYGGSV GAT +
Sbjct:   48 YIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDYGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCK 219

HSP 2 Score: 43.1282 bits (100), Expect = 7.967e-61
Identity = 19/20 (95.00%), Postives = 20/20 (100.00%), Query Frame = 2 
Query:  596 CKELAAQPDVDGFLVGGASL 655
            CKELA+QPDVDGFLVGGASL
Sbjct:  218 CKELASQPDVDGFLVGGASL 237
[Close]
BLAST of CK740263 vs. ExPASy Swiss-Prot
Match: TPIS_PANTR (Triosephosphate isomerase OS=Pan troglodytes GN=TPI1 PE=2 SV=2)

HSP 1 Score: 210.69 bits (535), Expect = 7.967e-61
Identity = 103/172 (59.88%), Postives = 125/172 (72.67%), Query Frame = 3 
Query:   87 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLGIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPAQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGATAR 602
            ++   +  L P   VAAQNC+    GAFTGEIS  M+ + G  WV+LGHSERR +  ES+E +G KVA+AL++GL VIAC+GE L++REAG T  VV  QTK IAD V  WS +VLAYEPVWAIGTGK ATP QAQEVH +LR WL +N S  +A +TRIIYGGSV GAT +
Sbjct:   48 YIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCK 219

HSP 2 Score: 43.1282 bits (100), Expect = 7.967e-61
Identity = 19/20 (95.00%), Postives = 20/20 (100.00%), Query Frame = 2 
Query:  596 CKELAAQPDVDGFLVGGASL 655
            CKELA+QPDVDGFLVGGASL
Sbjct:  218 CKELASQPDVDGFLVGGASL 237
[Close]
BLAST of CK740263 vs. ExPASy Swiss-Prot
Match: TPIS_MACMU (Triosephosphate isomerase OS=Macaca mulatta GN=TPI1 PE=3 SV=2)

HSP 1 Score: 210.69 bits (535), Expect = 7.967e-61
Identity = 103/172 (59.88%), Postives = 125/172 (72.67%), Query Frame = 3 
Query:   87 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLGIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPAQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGATAR 602
            ++   +  L P   VAAQNC+    GAFTGEIS  M+ + G  WV+LGHSERR +  ES+E +G KVA+AL++GL VIAC+GE L++REAG T  VV  QTK IAD V  WS +VLAYEPVWAIGTGK ATP QAQEVH +LR WL +N S  +A +TRIIYGGSV GAT +
Sbjct:   48 YIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSEAVAQSTRIIYGGSVTGATCK 219

HSP 2 Score: 43.1282 bits (100), Expect = 7.967e-61
Identity = 19/20 (95.00%), Postives = 20/20 (100.00%), Query Frame = 2 
Query:  596 CKELAAQPDVDGFLVGGASL 655
            CKELA+QPDVDGFLVGGASL
Sbjct:  218 CKELASQPDVDGFLVGGASL 237
[Close]
BLAST of CK740263 vs. ExPASy Swiss-Prot
Match: TPIS_MACFA (Triosephosphate isomerase OS=Macaca fascicularis GN=TPI1 PE=2 SV=3)

HSP 1 Score: 210.69 bits (535), Expect = 7.967e-61
Identity = 103/172 (59.88%), Postives = 125/172 (72.67%), Query Frame = 3 
Query:   87 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLGIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPAQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGATAR 602
            ++   +  L P   VAAQNC+    GAFTGEIS  M+ + G  WV+LGHSERR +  ES+E +G KVA+AL++GL VIAC+GE L++REAG T  VV  QTK IAD V  WS +VLAYEPVWAIGTGK ATP QAQEVH +LR WL +N S  +A +TRIIYGGSV GAT +
Sbjct:   48 YIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSEAVAQSTRIIYGGSVTGATCK 219

HSP 2 Score: 43.1282 bits (100), Expect = 7.967e-61
Identity = 19/20 (95.00%), Postives = 20/20 (100.00%), Query Frame = 2 
Query:  596 CKELAAQPDVDGFLVGGASL 655
            CKELA+QPDVDGFLVGGASL
Sbjct:  218 CKELASQPDVDGFLVGGASL 237
[Close]
BLAST of CK740263 vs. ExPASy Swiss-Prot
Match: TPIS_HUMAN (Triosephosphate isomerase OS=Homo sapiens GN=TPI1 PE=1 SV=2)

HSP 1 Score: 210.69 bits (535), Expect = 7.967e-61
Identity = 103/172 (59.88%), Postives = 125/172 (72.67%), Query Frame = 3 
Query:   87 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLGIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPAQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGATAR 602
            ++   +  L P   VAAQNC+    GAFTGEIS  M+ + G  WV+LGHSERR +  ES+E +G KVA+AL++GL VIAC+GE L++REAG T  VV  QTK IAD V  WS +VLAYEPVWAIGTGK ATP QAQEVH +LR WL +N S  +A +TRIIYGGSV GAT +
Sbjct:   48 YIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCK 219

HSP 2 Score: 43.1282 bits (100), Expect = 7.967e-61
Identity = 19/20 (95.00%), Postives = 20/20 (100.00%), Query Frame = 2 
Query:  596 CKELAAQPDVDGFLVGGASL 655
            CKELA+QPDVDGFLVGGASL
Sbjct:  218 CKELASQPDVDGFLVGGASL 237
The following BLAST results are available for this feature:
BLAST of CK740263 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
TPIC_SECCE1.631e-6463.16Triosephosphate isomerase, chloroplastic OS=Secale... [more]
TPIC_ARATH1.777e-6364.74Triosephosphate isomerase, chloroplastic OS=Arabid... [more]
TPIC_FRAAN5.137e-6357.79Triosephosphate isomerase, chloroplastic OS=Fragar... [more]
TPIS_CULTA3.300e-6262.79Triosephosphate isomerase OS=Culex tarsalis GN=Tpi... [more]
TPIS_RABIT3.595e-6160.47Triosephosphate isomerase OS=Oryctolagus cuniculus... [more]
TPIS_PONAB7.967e-6159.88Triosephosphate isomerase OS=Pongo abelii GN=TPI1 ... [more]
TPIS_PANTR7.967e-6159.88Triosephosphate isomerase OS=Pan troglodytes GN=TP... [more]
TPIS_MACMU7.967e-6159.88Triosephosphate isomerase OS=Macaca mulatta GN=TPI... [more]
TPIS_MACFA7.967e-6159.88Triosephosphate isomerase OS=Macaca fascicularis G... [more]
TPIS_HUMAN7.967e-6159.88Triosephosphate isomerase OS=Homo sapiens GN=TPI1 ... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_6405 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE63A10 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK740263 ID=CK740263; Name=CK740263; organism=Citrus sinensis; type=EST; length=721bp
AAGTGAAAAAGATAGTGAGCGTACTCAATGAAGGTCAAGTTCCATCTTCT
GATGTCGTTGAGGTCGTTGTTAGCCCTCCGTTTCTGTTTCTTGGACTGGT
GAAGAGTTCGTTGAGGCCTGATTTTCATGTTGCGGCACAGAATTGCTGGG
TCAAGAAGGGAGGTGCTTTCACCGGTGAGATTAGTGCGGAAATGCTCGTC
AATTTGGGTATTCCTTGGGTCATTCTTGGTCATTCTGAAAGGAGACTCAT
TTTGAATGAATCAAATGAGTTTGTTGGGGATAAGGTTGCATATGCACTTT
CTCAAGGTTTGAAGGTGATTGCTTGTGTTGGTGAGACACTTGAGCAGCGT
GAAGCAGGATCTACTATGGATGTTGTTGCTGCACAAACTAAAGCAATCGC
AGATCGAGTCTCAAGCTGGTCAAACATTGTTTTGGCCTATGAACCTGTGT
GGGCTATTGGAACTGGGAAGGTTGCCACTCCAGCGCAGGCTCAAGAAGTA
CATTTTGAACTGAGGAAATGGCTTTTTGCAAACACCAGTCCTGAAATTGC
TGCAGCAACACGGATCATCTACGGAGGATCCGTAAATGGTGCAACTGCAA
GGAATTGGCTGCACAGCCTGATGTTGACGGATTTTTGGTTGGTGGTGCTT
CGTTGAACCGGAGTTCATTGACATCATCAATCTGCTGAGCTGAAGTAAAA
TCTTGAGCTTTATTTCAAGGG
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