CK740285

Overview
NameCK740285
Unique NameCK740285
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length563
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig1234 contig Csv1_Contig1234:1050..1610. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK1_SOLLC (S-adenosylmethionine synthetase 1 OS=Solanum lycopersicum GN=SAM1 PE=2 SV=1)

HSP 1 Score: 185.652 bits (470), Expect = 1.080e-57
Identity = 86/91 (94.51%), Postives = 90/91 (98.90%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQ 375
            CIVQVSYAIGVPEPLSVFVD+YGTGKIPD+EILKIVKE+FDFRPGMM+INLDLKRGGN RFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQ
Sbjct:  302 CIVQVSYAIGVPEPLSVFVDTYGTGKIPDREILKIVKENFDFRPGMMSINLDLKRGGNRRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQ 392

HSP 2 Score: 57.7658 bits (138), Expect = 1.080e-57
Identity = 28/29 (96.55%), Postives = 29/29 (100.00%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRSGAYIVRQAAKSIVA+G
Sbjct:  269 AFSGKDPTKVDRSGAYIVRQAAKSIVASG 297
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK1_BRAJU (S-adenosylmethionine synthetase 1 OS=Brassica juncea GN=SAMS1 PE=2 SV=1)

HSP 1 Score: 184.496 bits (467), Expect = 1.836e-57
Identity = 86/91 (94.51%), Postives = 89/91 (97.80%), Query Frame = 1 
Query:  106 IVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 378
            +VQVSYAIGVPEP SVFVD+YGTG IPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKW+KPQA
Sbjct:  303 LVQVSYAIGVPEPFSVFVDTYGTGLIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWDKPQA 393

HSP 2 Score: 58.151 bits (139), Expect = 1.836e-57
Identity = 27/29 (93.10%), Postives = 29/29 (100.00%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRSGAYIVRQ+AKS+VANG
Sbjct:  269 AFSGKDPTKVDRSGAYIVRQSAKSVVANG 297
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK2_SOLLC (S-adenosylmethionine synthetase 2 OS=Solanum lycopersicum GN=SAM2 PE=2 SV=1)

HSP 1 Score: 182.956 bits (463), Expect = 1.836e-57
Identity = 84/92 (91.30%), Postives = 90/92 (97.83%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 378
            CIVQVSYAIGVPEPLSVFVD+YGTGKIPDKEIL IVKE+FDFRPGM++INLDL RGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKW+KP+A
Sbjct:  302 CIVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILNIVKENFDFRPGMISINLDLLRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWDKPEA 393

HSP 2 Score: 59.6918 bits (143), Expect = 1.836e-57
Identity = 29/29 (100.00%), Postives = 29/29 (100.00%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRSGAYIVRQAAKSIVANG
Sbjct:  269 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 297
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK1_ARATH (S-adenosylmethionine synthetase 1 OS=Arabidopsis thaliana GN=SAM1 PE=1 SV=2)

HSP 1 Score: 182.956 bits (463), Expect = 2.394e-57
Identity = 86/91 (94.51%), Postives = 89/91 (97.80%), Query Frame = 1 
Query:  106 IVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 378
            +VQVSYAIGVPEPLSVFVD+Y TG IPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKW+KPQA
Sbjct:  303 LVQVSYAIGVPEPLSVFVDTYETGLIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWDKPQA 393

HSP 2 Score: 59.3066 bits (142), Expect = 2.394e-57
Identity = 28/29 (96.55%), Postives = 29/29 (100.00%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRSGAYIVRQAAKS+VANG
Sbjct:  269 AFSGKDPTKVDRSGAYIVRQAAKSVVANG 297
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK2_ARATH (S-adenosylmethionine synthetase 2 OS=Arabidopsis thaliana GN=SAM2 PE=1 SV=1)

HSP 1 Score: 182.57 bits (462), Expect = 3.121e-57
Identity = 85/91 (93.41%), Postives = 89/91 (97.80%), Query Frame = 1 
Query:  106 IVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 378
            +VQVSYAIGVPEPLSVFVD+YGTG IPDKEILKIVKE+FDFRPGMMTINLDLKRGGNGRF KTAAYGHFGRDDPDFTWEVVKPLKW+KPQA
Sbjct:  303 LVQVSYAIGVPEPLSVFVDTYGTGLIPDKEILKIVKETFDFRPGMMTINLDLKRGGNGRFQKTAAYGHFGRDDPDFTWEVVKPLKWDKPQA 393

HSP 2 Score: 59.3066 bits (142), Expect = 3.121e-57
Identity = 28/29 (96.55%), Postives = 29/29 (100.00%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRSGAYIVRQAAKS+VANG
Sbjct:  269 AFSGKDPTKVDRSGAYIVRQAAKSVVANG 297
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK2_DAUCA (S-adenosylmethionine synthetase 2 OS=Daucus carota GN=SAMS2 PE=2 SV=1)

HSP 1 Score: 182.185 bits (461), Expect = 3.121e-57
Identity = 85/92 (92.39%), Postives = 88/92 (95.65%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 378
            CIVQVSYAIGVP+PLSVFVDSYGTG IPDKEILKIVKE+FDFRPGM+ INLDLKRGGN RFLKTAAYGHFGRDD DFTWEVVKPLKWEKPQA
Sbjct:  302 CIVQVSYAIGVPDPLSVFVDSYGTGNIPDKEILKIVKETFDFRPGMIAINLDLKRGGNNRFLKTAAYGHFGRDDADFTWEVVKPLKWEKPQA 393

HSP 2 Score: 59.6918 bits (143), Expect = 3.121e-57
Identity = 29/29 (100.00%), Postives = 29/29 (100.00%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRSGAYIVRQAAKSIVANG
Sbjct:  269 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 297
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK3_ACTCH (S-adenosylmethionine synthetase 3 (Fragment) OS=Actinidia chinensis GN=SAM3 PE=2 SV=1)

HSP 1 Score: 184.111 bits (466), Expect = 3.123e-57
Identity = 86/91 (94.51%), Postives = 89/91 (97.80%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQ 375
            CIVQVSYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKESFDFRPGM+ I+LDLKRGGNGRFLKTAAYGHFGRDD DFTWEVVKPLKWEKPQ
Sbjct:  269 CIVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKESFDFRPGMIAIHLDLKRGGNGRFLKTAAYGHFGRDDADFTWEVVKPLKWEKPQ 359

HSP 2 Score: 57.7658 bits (138), Expect = 3.123e-57
Identity = 28/29 (96.55%), Postives = 29/29 (100.00%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRSGAYIVRQAAKSIVA+G
Sbjct:  236 AFSGKDPTKVDRSGAYIVRQAAKSIVASG 264
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_SOLBR (S-adenosylmethionine synthetase OS=Solanum brevidens GN=SAMS PE=2 SV=1)

HSP 1 Score: 183.726 bits (465), Expect = 4.068e-57
Identity = 85/91 (93.41%), Postives = 89/91 (97.80%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQ 375
            CIVQVSYAIGVPEPLSVFVD+YGTGKIPD+EILKIVKE+FDFRPGMM+INLDLKRGGNGRFLKTAAYGHFGRDD DFTWEVVKPLKWE PQ
Sbjct:  302 CIVQVSYAIGVPEPLSVFVDTYGTGKIPDREILKIVKENFDFRPGMMSINLDLKRGGNGRFLKTAAYGHFGRDDADFTWEVVKPLKWENPQ 392

HSP 2 Score: 57.7658 bits (138), Expect = 4.068e-57
Identity = 28/29 (96.55%), Postives = 29/29 (100.00%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRSGAYIVRQAAKSIVA+G
Sbjct:  269 AFSGKDPTKVDRSGAYIVRQAAKSIVASG 297
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_LITCN (S-adenosylmethionine synthetase OS=Litchi chinensis GN=SAMS PE=2 SV=1)

HSP 1 Score: 183.341 bits (464), Expect = 5.304e-57
Identity = 84/92 (91.30%), Postives = 91/92 (98.91%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 378
            CIVQVSYAIGVPEPLSVFVDSYGTGKIPD+EILKIVKE+FDFRPGM+++NLDLKRGGNGRFLKTAAYGHFGR+DPDFTWEVVKPLKW+K QA
Sbjct:  302 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDREILKIVKENFDFRPGMISVNLDLKRGGNGRFLKTAAYGHFGREDPDFTWEVVKPLKWDKVQA 393

HSP 2 Score: 57.7658 bits (138), Expect = 5.304e-57
Identity = 28/29 (96.55%), Postives = 29/29 (100.00%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRSGAYIVRQAAKSIVA+G
Sbjct:  269 AFSGKDPTKVDRSGAYIVRQAAKSIVASG 297
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK1_CATRO (S-adenosylmethionine synthetase 1 OS=Catharanthus roseus GN=SAMS1 PE=1 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 5.304e-57
Identity = 84/89 (94.38%), Postives = 88/89 (98.88%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEK 369
            CIVQVSYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKE+FDFRPGM+ INLDLKRGG+GRFLKTAAYGHFGRDDPDFTWEVVKPLKWEK
Sbjct:  302 CIVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKENFDFRPGMIAINLDLKRGGSGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEK 390

HSP 2 Score: 59.6918 bits (143), Expect = 5.304e-57
Identity = 29/29 (100.00%), Postives = 29/29 (100.00%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRSGAYIVRQAAKSIVANG
Sbjct:  269 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 297
The following BLAST results are available for this feature:
BLAST of CK740285 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK1_SOLLC1.080e-5794.51S-adenosylmethionine synthetase 1 OS=Solanum lycop... [more]
METK1_BRAJU1.836e-5794.51S-adenosylmethionine synthetase 1 OS=Brassica junc... [more]
METK2_SOLLC1.836e-5791.30S-adenosylmethionine synthetase 2 OS=Solanum lycop... [more]
METK1_ARATH2.394e-5794.51S-adenosylmethionine synthetase 1 OS=Arabidopsis t... [more]
METK2_ARATH3.121e-5793.41S-adenosylmethionine synthetase 2 OS=Arabidopsis t... [more]
METK2_DAUCA3.121e-5792.39S-adenosylmethionine synthetase 2 OS=Daucus carota... [more]
METK3_ACTCH3.123e-5794.51S-adenosylmethionine synthetase 3 (Fragment) OS=Ac... [more]
METK_SOLBR4.068e-5793.41S-adenosylmethionine synthetase OS=Solanum brevide... [more]
METK_LITCN5.304e-5791.30S-adenosylmethionine synthetase OS=Litchi chinensi... [more]
METK1_CATRO5.304e-5794.38S-adenosylmethionine synthetase 1 OS=Catharanthus ... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_6428 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE63C12 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK740285 ID=CK740285; Name=CK740285; organism=Citrus sinensis; type=EST; length=563bp
GTGCTTTCTCTGGAAAAGACCCTACTAAGGTGGATAGGAGTGGTGCATAC
ATTGTTAGGCAGGCTGCTAAGAGCATTGTTGCAAACGGGCCTTGCTCGCA
GGTGCATTGTCCAAGTCTCCTATGCCATTGGTGTGCCTGAGCCATTGTCT
GTTTTTGTGGACTCATATGGAACCGGCAAGATTCCTGACAAGGAGATCCT
CAAAATTGTGAAGGAAAGCTTTGATTTCAGGCCAGGAATGATGACCATCA
ATTTGGATCTCAAAAGGGGCGGTAATGGCAGGTTCTTGAAGACTGCTGCC
TATGGACACTTTGGCAGGGATGACCCAGACTTCACTTGGGAAGTTGTGAA
GCCCCTCAAGTGGGAAAAGCCTCAAGCTTAAATTTGAGTTGAAAAGTGAA
AACTATCCCTGCTGTTCAAGTTTGCTTCTATTGGTAGTGGATGAATAATT
TGAATATTATCTTGCTGCTGCTTTGTAATACTAAGCAAATCTCCTGTGTT
CAGAACTCATACATGTTCCCCCATTCAATTCCTTTCTTTTTCTTCTTTCC
CACTTCTTTTTTG
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