CK740285 | Citrus Genome Database

Overview


Name	CK740285
Unique Name	CK740285
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	563

Libraries


Library Name	Type
Ridge pineapple sweet orange entire seedling	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_TERTT (S-adenosylmethionine synthetase OS=Teredinibacter turnerae (strain ATCC 39867 / T7901) GN=metK PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 9.519e-26
Identity = 46/81 (56.79%), Postives = 60/81 (74.07%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEV 345
            C +QVSYAIGV EP S+ V+++GTGKI D +I+++VKE FD RP  +   LDL R     + KTAAYGHFGR+ P+FTWE+
Sbjct:  296 CEIQVSYAIGVAEPTSISVNTFGTGKIDDGKIVELVKEHFDLRPRGLIEMLDLMR---PIYRKTAAYGHFGRELPEFTWEI 373

HSP 2 Score: 43.1282 bits (100), Expect = 9.519e-26
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK+IVA G
Sbjct:  263 AFSGKDPSKVDRSAAYAGRYVAKNIVAAG 291

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK5_CAEEL (Probable S-adenosylmethionine synthetase 5 OS=Caenorhabditis elegans GN=sams-5 PE=2 SV=2)

HSP 1 Score: 89.7373 bits (221), Expect = 1.238e-25
Identity = 48/89 (53.93%), Postives = 61/89 (68.54%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEK 369
            C+VQVSYAIGV +PLSV V S+GT  + + E+LKIV ++FD RPGM+  NLDLKR     +  TA  GHFG +  DF WE  + LK +K
Sbjct:  302 CLVQVSYAIGVAKPLSVMVFSFGTSALNEAELLKIVNDNFDLRPGMIIKNLDLKR---AIYEPTAENGHFGHN--DFPWEQARKLKIDK 385

HSP 2 Score: 46.2098 bits (108), Expect = 1.238e-25
Identity = 22/31 (70.97%), Postives = 23/31 (74.19%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANGPC 95
            AFSGKDPTKVDRS AY  R  AKS+V  G C
Sbjct:  269 AFSGKDPTKVDRSAAYAARWVAKSLVKAGLC 299

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_COXBU (S-adenosylmethionine synthetase OS=Coxiella burnetii GN=metK PE=3 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.238e-25
Identity = 42/80 (52.50%), Postives = 57/80 (71.25%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ V+++GTGKI +  I +++KE FD RPG +   L+L R     +  TA YGHFGR++P+FTWE
Sbjct:  296 CEIQVSYAIGVAEPTSISVETFGTGKIDEVRIQQLIKEHFDLRPGKIIEELNLLR---SIYKATATYGHFGREEPEFTWE 372

HSP 2 Score: 45.8246 bits (107), Expect = 1.238e-25
Identity = 21/28 (75.00%), Postives = 23/28 (82.14%), Query Frame = 3

Query:    6 FSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            FSGKDP+KVDRSGAY  R  AK+IVA G
Sbjct:  264 FSGKDPSKVDRSGAYAARYVAKNIVAGG 291

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_COXBR (S-adenosylmethionine synthetase OS=Coxiella burnetii (strain RSA 331 / Henzerling II) GN=metK PE=3 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.238e-25
Identity = 42/80 (52.50%), Postives = 57/80 (71.25%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ V+++GTGKI +  I +++KE FD RPG +   L+L R     +  TA YGHFGR++P+FTWE
Sbjct:  296 CEIQVSYAIGVAEPTSISVETFGTGKIDEVRIQQLIKEHFDLRPGKIIEELNLLR---SIYKATATYGHFGREEPEFTWE 372

HSP 2 Score: 45.8246 bits (107), Expect = 1.238e-25
Identity = 21/28 (75.00%), Postives = 23/28 (82.14%), Query Frame = 3

Query:    6 FSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            FSGKDP+KVDRSGAY  R  AK+IVA G
Sbjct:  264 FSGKDPSKVDRSGAYAARYVAKNIVAGG 291

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_COXB2 (S-adenosylmethionine synthetase OS=Coxiella burnetii (strain CbuG_Q212) GN=metK PE=3 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.238e-25
Identity = 42/80 (52.50%), Postives = 57/80 (71.25%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ V+++GTGKI +  I +++KE FD RPG +   L+L R     +  TA YGHFGR++P+FTWE
Sbjct:  296 CEIQVSYAIGVAEPTSISVETFGTGKIDEVRIQQLIKEHFDLRPGKIIEELNLLR---SIYKATATYGHFGREEPEFTWE 372

HSP 2 Score: 45.8246 bits (107), Expect = 1.238e-25
Identity = 21/28 (75.00%), Postives = 23/28 (82.14%), Query Frame = 3

Query:    6 FSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            FSGKDP+KVDRSGAY  R  AK+IVA G
Sbjct:  264 FSGKDPSKVDRSGAYAARYVAKNIVAGG 291

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_COXB1 (S-adenosylmethionine synthetase OS=Coxiella burnetii (strain CbuK_Q154) GN=metK PE=3 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.238e-25
Identity = 42/80 (52.50%), Postives = 57/80 (71.25%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ V+++GTGKI +  I +++KE FD RPG +   L+L R     +  TA YGHFGR++P+FTWE
Sbjct:  296 CEIQVSYAIGVAEPTSISVETFGTGKIDEVRIQQLIKEHFDLRPGKIIEELNLLR---SIYKATATYGHFGREEPEFTWE 372

HSP 2 Score: 45.8246 bits (107), Expect = 1.238e-25
Identity = 21/28 (75.00%), Postives = 23/28 (82.14%), Query Frame = 3

Query:    6 FSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            FSGKDP+KVDRSGAY  R  AK+IVA G
Sbjct:  264 FSGKDPSKVDRSGAYAARYVAKNIVAGG 291

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_ACACA (S-adenosylmethionine synthetase OS=Acanthamoeba castellanii GN=metK PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 1.239e-25
Identity = 49/88 (55.68%), Postives = 61/88 (69.32%), Query Frame = 1

Query:  106 IVQVSYAIGVPEPLSVFVDSYGT--GKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKW 363
            +VQVSYAIGV  PLSVFVDSYGT  G   D+++L I K +FD RPG +  +L L+R     + KTA +GHFGR+DPDF WE  K L +
Sbjct:  304 LVQVSYAIGVSHPLSVFVDSYGTAQGGRTDEDLLAISKSNFDLRPGKIINDLQLRR---PIYEKTAYHGHFGRNDPDFLWEAPKKLNF 388

HSP 2 Score: 44.669 bits (104), Expect = 1.239e-25
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AKS+VA G
Sbjct:  270 AFSGKDPSKVDRSAAYAARWIAKSLVARG 298

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_POLSQ (S-adenosylmethionine synthetase OS=Polynucleobacter sp. (strain QLW-P1DMWA-1) GN=metK PE=3 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 1.612e-25
Identity = 43/80 (53.75%), Postives = 60/80 (75.00%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C++Q+SYAIGV +P SV V ++GTGKI D++I ++V E+FD RP  +   L+L R     + KTAAYGHFGR++P+FTWE
Sbjct:  298 CLIQISYAIGVAKPTSVMVSTFGTGKISDEKIAQLVSENFDLRPKGIVKMLNLLR---PIYRKTAAYGHFGREEPEFTWE 374

HSP 2 Score: 43.5134 bits (101), Expect = 1.612e-25
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQ 101
            AFSGKDP+KVDRS AY  R  AK++VA G  S+
Sbjct:  265 AFSGKDPSKVDRSAAYAGRYVAKNVVAAGLASK 297

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_METFK (S-adenosylmethionine synthetase OS=Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) GN=metK PE=3 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 1.612e-25
Identity = 44/80 (55.00%), Postives = 59/80 (73.75%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C+VQVSYAIGV +P SV V++YGTGK+ ++ + ++V + FD RP  +   LDL R     + KTAAYGHFGRD+P+FTWE
Sbjct:  297 CLVQVSYAIGVAKPTSVMVETYGTGKVSNEILTQLVLDHFDLRPKGIVKMLDLLR---PIYAKTAAYGHFGRDEPEFTWE 373

HSP 2 Score: 43.8986 bits (102), Expect = 1.612e-25
Identity = 22/33 (66.67%), Postives = 25/33 (75.76%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQ 101
            AFSGKDP+KVDRS AY  R  AK+IVA G  S+
Sbjct:  264 AFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASR 296

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_PELCD (S-adenosylmethionine synthetase OS=Pelobacter carbinolicus (strain DSM 2380 / Gra Bd 1) GN=metK PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 2.098e-25
Identity = 44/80 (55.00%), Postives = 58/80 (72.50%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C VQ++YAIGV EP+S+ V+++GTG +P  EI +IV+E FD RP  +   LDL R     + KTAAYGHFGR+ P+FTWE
Sbjct:  299 CEVQIAYAIGVAEPVSIMVNTFGTGVLPSNEIARIVREEFDMRPAAIIETLDLLR---PIYRKTAAYGHFGRELPEFTWE 375

HSP 2 Score: 41.9726 bits (97), Expect = 2.098e-25
Identity = 18/27 (66.67%), Postives = 23/27 (85.19%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVA 83
            AFSGKDP+KVDRS +Y+ R  AK++VA
Sbjct:  266 AFSGKDPSKVDRSASYMARYVAKNVVA 292

The following BLAST results are available for this feature:

BLAST of CK740285 vs. ExPASy Swiss-Prot

BLAST of CK740285 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
METK_TERTT	9.519e-26	56.79	S-adenosylmethionine synthetase OS=Teredinibacter ...	[more]
METK5_CAEEL	1.238e-25	53.93	Probable S-adenosylmethionine synthetase 5 OS=Caen...	[more]
METK_COXBU	1.238e-25	52.50	S-adenosylmethionine synthetase OS=Coxiella burnet...	[more]
METK_COXBR	1.238e-25	52.50	S-adenosylmethionine synthetase OS=Coxiella burnet...	[more]
METK_COXB2	1.238e-25	52.50	S-adenosylmethionine synthetase OS=Coxiella burnet...	[more]
METK_COXB1	1.238e-25	52.50	S-adenosylmethionine synthetase OS=Coxiella burnet...	[more]
METK_ACACA	1.239e-25	55.68	S-adenosylmethionine synthetase OS=Acanthamoeba ca...	[more]
METK_POLSQ	1.612e-25	53.75	S-adenosylmethionine synthetase OS=Polynucleobacte...	[more]
METK_METFK	1.612e-25	55.00	S-adenosylmethionine synthetase OS=Methylobacillus...	[more]
METK_PELCD	2.098e-25	55.00	S-adenosylmethionine synthetase OS=Pelobacter carb...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_6428 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE63C12 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK740285 ID=CK740285; Name=CK740285; organism=Citrus sinensis; type=EST; length=563bp
GTGCTTTCTCTGGAAAAGACCCTACTAAGGTGGATAGGAGTGGTGCATAC
ATTGTTAGGCAGGCTGCTAAGAGCATTGTTGCAAACGGGCCTTGCTCGCA
GGTGCATTGTCCAAGTCTCCTATGCCATTGGTGTGCCTGAGCCATTGTCT
GTTTTTGTGGACTCATATGGAACCGGCAAGATTCCTGACAAGGAGATCCT
CAAAATTGTGAAGGAAAGCTTTGATTTCAGGCCAGGAATGATGACCATCA
ATTTGGATCTCAAAAGGGGCGGTAATGGCAGGTTCTTGAAGACTGCTGCC
TATGGACACTTTGGCAGGGATGACCCAGACTTCACTTGGGAAGTTGTGAA
GCCCCTCAAGTGGGAAAAGCCTCAAGCTTAAATTTGAGTTGAAAAGTGAA
AACTATCCCTGCTGTTCAAGTTTGCTTCTATTGGTAGTGGATGAATAATT
TGAATATTATCTTGCTGCTGCTTTGTAATACTAAGCAAATCTCCTGTGTT
CAGAACTCATACATGTTCCCCCATTCAATTCCTTTCTTTTTCTTCTTTCC
CACTTCTTTTTTG