CK740285

Overview
NameCK740285
Unique NameCK740285
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length563
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_NEIG2 (S-adenosylmethionine synthetase OS=Neisseria gonorrhoeae (strain NCCP11945) GN=metK PE=3 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 2.098e-25
Identity = 43/80 (53.75%), Postives = 59/80 (73.75%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ +D++GTGKI +++++ +V E FD RP  +   LDL R   G   K+AAYGHFGR++P+FTWE
Sbjct:  299 CQIQVSYAIGVAEPTSISIDTFGTGKISEEKLIALVCEHFDLRPKGIVQMLDLLRPIYG---KSAAYGHFGREEPEFTWE 375

HSP 2 Score: 45.0542 bits (105), Expect = 2.098e-25
Identity = 22/33 (66.67%), Postives = 25/33 (75.76%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQ 101
            AFSGKDP+KVDRS AY  R  AK+IVA G  +Q
Sbjct:  266 AFSGKDPSKVDRSAAYACRYVAKNIVAAGLATQ 298
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_NEIG1 (S-adenosylmethionine synthetase OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) GN=metK PE=3 SV=2)

HSP 1 Score: 90.1225 bits (222), Expect = 2.098e-25
Identity = 43/80 (53.75%), Postives = 59/80 (73.75%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ +D++GTGKI +++++ +V E FD RP  +   LDL R   G   K+AAYGHFGR++P+FTWE
Sbjct:  299 CQIQVSYAIGVAEPTSISIDTFGTGKISEEKLIALVCEHFDLRPKGIVQMLDLLRPIYG---KSAAYGHFGREEPEFTWE 375

HSP 2 Score: 45.0542 bits (105), Expect = 2.098e-25
Identity = 22/33 (66.67%), Postives = 25/33 (75.76%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQ 101
            AFSGKDP+KVDRS AY  R  AK+IVA G  +Q
Sbjct:  266 AFSGKDPSKVDRSAAYACRYVAKNIVAAGLATQ 298
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_DESMR (S-adenosylmethionine synthetase OS=Desulfovibrio magneticus (strain ATCC 700980 / DSM 13731 / RS-1) GN=metK PE=3 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 2.098e-25
Identity = 42/81 (51.85%), Postives = 53/81 (65.43%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEV 345
            C VQ++Y IGV EPLSV V S G+  IPD+ + K V+E FD RP  ++  LDLKR     +  T+ YGHFGR+   FTWEV
Sbjct:  299 CEVQIAYCIGVAEPLSVLVTSMGSSDIPDEALTKAVREVFDLRPYYISKRLDLKR---PIYKPTSCYGHFGREQAGFTWEV 376

HSP 2 Score: 51.6026 bits (122), Expect = 2.098e-25
Identity = 23/41 (56.10%), Postives = 29/41 (70.73%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQVHCPSLLC 125
            AFSGKDP+KVDRSGAY+ R  AK++VA+G C +       C
Sbjct:  266 AFSGKDPSKVDRSGAYMARYVAKNMVASGACKRCEVQIAYC 306
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_FRAP2 (S-adenosylmethionine synthetase OS=Francisella philomiragia subsp. philomiragia (strain ATCC 25017) GN=metK PE=3 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 2.099e-25
Identity = 44/82 (53.66%), Postives = 60/82 (73.17%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVV 348
            C VQV+YAIGV +P+S+ V+++GTGKI D +I K+V E+FD R G +  NLDL R     + KT+ YGHFGR+ P+FTWE +
Sbjct:  296 CEVQVAYAIGVAKPVSLMVNTFGTGKISDSKIEKLVSETFDLRVGKIIENLDLLR---PIYRKTSNYGHFGRELPEFTWEKI 374

HSP 2 Score: 45.4394 bits (106), Expect = 2.099e-25
Identity = 21/29 (72.41%), Postives = 25/29 (86.21%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRSGAY+ R  AK++VA G
Sbjct:  263 AFSGKDPSKVDRSGAYMGRYIAKNVVAAG 291
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_THESQ (S-adenosylmethionine synthetase OS=Thermotoga sp. (strain RQ2) GN=metK PE=3 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 2.730e-25
Identity = 40/79 (50.63%), Postives = 60/79 (75.95%), Query Frame = 1 
Query:  106 IVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            ++Q+SYAIGV +P+S+ +D+YGT K+ + ++LK++ E FDFRPG +   L+L R     + KTAAYGHFGR++ +FTWE
Sbjct:  306 LIQLSYAIGVAKPVSIMIDTYGTAKVDEDKLLKVITELFDFRPGAIIKKLNLLR---PIYRKTAAYGHFGRNEEEFTWE 381

HSP 2 Score: 43.8986 bits (102), Expect = 2.730e-25
Identity = 20/29 (68.97%), Postives = 23/29 (79.31%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRS  Y+ R  AK++VA G
Sbjct:  272 AFSGKDPTKVDRSAHYMARYVAKNVVAAG 300
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_THEP1 (S-adenosylmethionine synthetase OS=Thermotoga petrophila (strain RKU-1 / ATCC BAA-488 / DSM 13995) GN=metK PE=3 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 2.730e-25
Identity = 40/79 (50.63%), Postives = 60/79 (75.95%), Query Frame = 1 
Query:  106 IVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            ++Q+SYAIGV +P+S+ +D+YGT K+ + ++LK++ E FDFRPG +   L+L R     + KTAAYGHFGR++ +FTWE
Sbjct:  306 LIQLSYAIGVAKPVSIMIDTYGTAKVDEDKLLKVITELFDFRPGAIIKKLNLLR---PIYRKTAAYGHFGRNEEEFTWE 381

HSP 2 Score: 43.8986 bits (102), Expect = 2.730e-25
Identity = 20/29 (68.97%), Postives = 23/29 (79.31%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRS  Y+ R  AK++VA G
Sbjct:  272 AFSGKDPTKVDRSAHYMARYVAKNVVAAG 300
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_THEMA (S-adenosylmethionine synthetase OS=Thermotoga maritima GN=metK PE=3 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 2.730e-25
Identity = 40/79 (50.63%), Postives = 60/79 (75.95%), Query Frame = 1 
Query:  106 IVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            ++Q+SYAIGV +P+S+ +D+YGT K+ + ++LK++ E FDFRPG +   L+L R     + KTAAYGHFGR++ +FTWE
Sbjct:  306 LIQLSYAIGVAKPVSIMIDTYGTAKVDEDKLLKVITELFDFRPGAIIKKLNLLR---PIYRKTAAYGHFGRNEEEFTWE 381

HSP 2 Score: 43.8986 bits (102), Expect = 2.730e-25
Identity = 20/29 (68.97%), Postives = 23/29 (79.31%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRS  Y+ R  AK++VA G
Sbjct:  272 AFSGKDPTKVDRSAHYMARYVAKNVVAAG 300
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_LARHH (S-adenosylmethionine synthetase OS=Laribacter hongkongensis (strain HLHK9) GN=metK PE=3 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 2.731e-25
Identity = 43/80 (53.75%), Postives = 59/80 (73.75%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIG+ EP S+ VD++GT  IP+++I+++VK  FD RP  +   LDL R     + KTAAYGHFGR++P+FTWE
Sbjct:  299 CQIQVSYAIGIAEPTSISVDTFGTNAIPNEKIVELVKRHFDLRPKGIIQMLDLLR---PIYTKTAAYGHFGREEPEFTWE 375

HSP 2 Score: 43.5134 bits (101), Expect = 2.731e-25
Identity = 22/33 (66.67%), Postives = 24/33 (72.73%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQ 101
            AFSGKDP+KVDRS AY  R  AK+IVA G   Q
Sbjct:  266 AFSGKDPSKVDRSAAYAGRYVAKNIVAAGLARQ 298
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_MAGSM (S-adenosylmethionine synthetase OS=Magnetococcus sp. (strain MC-1) GN=metK PE=3 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 2.731e-25
Identity = 43/80 (53.75%), Postives = 59/80 (73.75%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QV+YAIGV +P+SV VD++GTGK+ D+ I ++VK+ FD RP  +   LDL R     + K+AAYGHFGR+ P+FTWE
Sbjct:  299 CEIQVAYAIGVSQPMSVMVDTFGTGKLDDEAITELVKKHFDLRPKAIVQQLDLLR---PIYKKSAAYGHFGRELPEFTWE 375

HSP 2 Score: 43.8986 bits (102), Expect = 2.731e-25
Identity = 21/29 (72.41%), Postives = 24/29 (82.76%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY+ R  AK+IVA G
Sbjct:  266 AFSGKDPSKVDRSSAYMGRYVAKNIVAAG 294
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_BORPE (S-adenosylmethionine synthetase OS=Bordetella pertussis GN=metK PE=3 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 2.731e-25
Identity = 45/80 (56.25%), Postives = 58/80 (72.50%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C VQVSYAIGV EP+++ V + GTG IPD++I K+V+E FD RP  +   LDL R     + KTAAYGHFGR +P+F+WE
Sbjct:  299 CQVQVSYAIGVAEPINITVYTEGTGVIPDEQIAKLVREHFDLRPKGIVNMLDLLR---PIYTKTAAYGHFGRSEPEFSWE 375

HSP 2 Score: 44.2838 bits (103), Expect = 2.731e-25
Identity = 21/33 (63.64%), Postives = 24/33 (72.73%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQ 101
            AFSGKDP+KVDRS AY  R  AK++VA G   Q
Sbjct:  266 AFSGKDPSKVDRSAAYAARYVAKNVVAAGLARQ 298
The following BLAST results are available for this feature:
BLAST of CK740285 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK_NEIG22.098e-2553.75S-adenosylmethionine synthetase OS=Neisseria gonor... [more]
METK_NEIG12.098e-2553.75S-adenosylmethionine synthetase OS=Neisseria gonor... [more]
METK_DESMR2.098e-2551.85S-adenosylmethionine synthetase OS=Desulfovibrio m... [more]
METK_FRAP22.099e-2553.66S-adenosylmethionine synthetase OS=Francisella phi... [more]
METK_THESQ2.730e-2550.63S-adenosylmethionine synthetase OS=Thermotoga sp. ... [more]
METK_THEP12.730e-2550.63S-adenosylmethionine synthetase OS=Thermotoga petr... [more]
METK_THEMA2.730e-2550.63S-adenosylmethionine synthetase OS=Thermotoga mari... [more]
METK_LARHH2.731e-2553.75S-adenosylmethionine synthetase OS=Laribacter hong... [more]
METK_MAGSM2.731e-2553.75S-adenosylmethionine synthetase OS=Magnetococcus s... [more]
METK_BORPE2.731e-2556.25S-adenosylmethionine synthetase OS=Bordetella pert... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_6428 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE63C12 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK740285 ID=CK740285; Name=CK740285; organism=Citrus sinensis; type=EST; length=563bp
GTGCTTTCTCTGGAAAAGACCCTACTAAGGTGGATAGGAGTGGTGCATAC
ATTGTTAGGCAGGCTGCTAAGAGCATTGTTGCAAACGGGCCTTGCTCGCA
GGTGCATTGTCCAAGTCTCCTATGCCATTGGTGTGCCTGAGCCATTGTCT
GTTTTTGTGGACTCATATGGAACCGGCAAGATTCCTGACAAGGAGATCCT
CAAAATTGTGAAGGAAAGCTTTGATTTCAGGCCAGGAATGATGACCATCA
ATTTGGATCTCAAAAGGGGCGGTAATGGCAGGTTCTTGAAGACTGCTGCC
TATGGACACTTTGGCAGGGATGACCCAGACTTCACTTGGGAAGTTGTGAA
GCCCCTCAAGTGGGAAAAGCCTCAAGCTTAAATTTGAGTTGAAAAGTGAA
AACTATCCCTGCTGTTCAAGTTTGCTTCTATTGGTAGTGGATGAATAATT
TGAATATTATCTTGCTGCTGCTTTGTAATACTAAGCAAATCTCCTGTGTT
CAGAACTCATACATGTTCCCCCATTCAATTCCTTTCTTTTTCTTCTTTCC
CACTTCTTTTTTG
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