CK740285 | Citrus Genome Database

Overview


Name	CK740285
Unique Name	CK740285
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	563

Libraries


Library Name	Type
Ridge pineapple sweet orange entire seedling	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_BORPA (S-adenosylmethionine synthetase OS=Bordetella parapertussis GN=metK PE=3 SV=2)

HSP 1 Score: 90.5077 bits (223), Expect = 2.731e-25
Identity = 45/80 (56.25%), Postives = 58/80 (72.50%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C VQVSYAIGV EP+++ V + GTG IPD++I K+V+E FD RP  +   LDL R     + KTAAYGHFGR +P+F+WE
Sbjct:  299 CQVQVSYAIGVAEPINITVYTEGTGVIPDEQIAKLVREHFDLRPKGIVNMLDLLR---PIYTKTAAYGHFGRSEPEFSWE 375

HSP 2 Score: 44.2838 bits (103), Expect = 2.731e-25
Identity = 21/33 (63.64%), Postives = 24/33 (72.73%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQ 101
            AFSGKDP+KVDRS AY  R  AK++VA G   Q
Sbjct:  266 AFSGKDPSKVDRSAAYAARYVAKNVVAAGLARQ 298

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_BORBR (S-adenosylmethionine synthetase OS=Bordetella bronchiseptica GN=metK PE=3 SV=2)

HSP 1 Score: 90.5077 bits (223), Expect = 2.731e-25
Identity = 45/80 (56.25%), Postives = 58/80 (72.50%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C VQVSYAIGV EP+++ V + GTG IPD++I K+V+E FD RP  +   LDL R     + KTAAYGHFGR +P+F+WE
Sbjct:  299 CQVQVSYAIGVAEPINITVYTEGTGVIPDEQIAKLVREHFDLRPKGIVNMLDLLR---PIYTKTAAYGHFGRSEPEFSWE 375

HSP 2 Score: 44.2838 bits (103), Expect = 2.731e-25
Identity = 21/33 (63.64%), Postives = 24/33 (72.73%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQ 101
            AFSGKDP+KVDRS AY  R  AK++VA G   Q
Sbjct:  266 AFSGKDPSKVDRSAAYAARYVAKNVVAAGLARQ 298

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_SACD2 (S-adenosylmethionine synthetase OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=metK PE=3 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 2.732e-25
Identity = 45/80 (56.25%), Postives = 57/80 (71.25%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ V+++GTG I D EI K+V + FD RP  +   LDL R     + KTAAYGHFGR++P+FTWE
Sbjct:  296 CEIQVSYAIGVAEPTSISVNTFGTGSIADSEISKLVAQHFDLRPRGIIEMLDLLR---PIYRKTAAYGHFGREEPEFTWE 372

HSP 2 Score: 43.8986 bits (102), Expect = 2.732e-25
Identity = 22/33 (66.67%), Postives = 25/33 (75.76%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQ 101
            AFSGKDP+KVDRS AY  R  AK+IVA G  S+
Sbjct:  263 AFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASR 295

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_CALS8 (S-adenosylmethionine synthetase OS=Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903) GN=metK PE=3 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 3.553e-25
Identity = 45/80 (56.25%), Postives = 57/80 (71.25%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C VQ+SYAIGV  PLS+ VD++GTGKI D++I +IVK  FD RP  +  +LDL+R     + K AAYGHFGR+D D  WE
Sbjct:  305 CEVQLSYAIGVARPLSIRVDTFGTGKIDDEKIAEIVKRVFDLRPAAIIRDLDLRR---PIYKKVAAYGHFGREDLDLPWE 381

HSP 2 Score: 44.2838 bits (103), Expect = 3.553e-25
Identity = 21/29 (72.41%), Postives = 22/29 (75.86%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRS  Y  R  AK+IVA G
Sbjct:  272 AFSGKDPTKVDRSATYAARYVAKNIVAAG 300

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_CLOCE (S-adenosylmethionine synthetase OS=Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10) GN=metK PE=3 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 3.553e-25
Identity = 41/80 (51.25%), Postives = 59/80 (73.75%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C VQ++YAIGV +P+SV VD++GT  IP+++I ++V + FD RP  +   L+L+R     + KTAAYGHFGRDD +F+WE
Sbjct:  306 CEVQLAYAIGVAKPVSVLVDTFGTAVIPEEKISELVNKHFDLRPAAIINKLNLRR---PIYRKTAAYGHFGRDDAEFSWE 382

HSP 2 Score: 46.9802 bits (110), Expect = 3.553e-25
Identity = 22/29 (75.86%), Postives = 24/29 (82.76%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRS AY  R  AK+IVA+G
Sbjct:  273 AFSGKDPTKVDRSAAYAARYVAKNIVASG 301

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK2_PONAB (S-adenosylmethionine synthetase isoform type-2 OS=Pongo abelii GN=MAT2A PE=2 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 3.554e-25
Identity = 46/86 (53.49%), Postives = 63/86 (73.26%), Query Frame = 1

Query:  106 IVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKW 363
            +VQVSYAIGV  PLS+ +  YGT +  ++E+L+IVK++FD RPG++  +LDLK+     + +TAAYGHFGRD   F WEV K LK+
Sbjct:  315 LVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKKNFDLRPGVIVRDLDLKK---PIYQRTAAYGHFGRD--SFPWEVPKKLKY 395

HSP 2 Score: 44.669 bits (104), Expect = 3.554e-25
Identity = 22/34 (64.71%), Postives = 24/34 (70.59%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQV 104
            AFSGKD TKVDRS AY  R  AKS+V  G C +V
Sbjct:  281 AFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRV 314

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK2_MOUSE (S-adenosylmethionine synthetase isoform type-2 OS=Mus musculus GN=Mat2a PE=2 SV=2)

HSP 1 Score: 89.7373 bits (221), Expect = 3.554e-25
Identity = 46/86 (53.49%), Postives = 63/86 (73.26%), Query Frame = 1

Query:  106 IVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKW 363
            +VQVSYAIGV  PLS+ +  YGT +  ++E+L+IVK++FD RPG++  +LDLK+     + +TAAYGHFGRD   F WEV K LK+
Sbjct:  315 LVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKKNFDLRPGVIVRDLDLKK---PIYQRTAAYGHFGRD--SFPWEVPKKLKY 395

HSP 2 Score: 44.669 bits (104), Expect = 3.554e-25
Identity = 22/34 (64.71%), Postives = 24/34 (70.59%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQV 104
            AFSGKD TKVDRS AY  R  AKS+V  G C +V
Sbjct:  281 AFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRV 314

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK2_MACFA (S-adenosylmethionine synthetase isoform type-2 OS=Macaca fascicularis GN=MAT2A PE=2 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 3.554e-25
Identity = 46/86 (53.49%), Postives = 63/86 (73.26%), Query Frame = 1

Query:  106 IVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKW 363
            +VQVSYAIGV  PLS+ +  YGT +  ++E+L+IVK++FD RPG++  +LDLK+     + +TAAYGHFGRD   F WEV K LK+
Sbjct:  315 LVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKKNFDLRPGVIVRDLDLKK---PIYQRTAAYGHFGRD--SFPWEVPKKLKY 395

HSP 2 Score: 44.669 bits (104), Expect = 3.554e-25
Identity = 22/34 (64.71%), Postives = 24/34 (70.59%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQV 104
            AFSGKD TKVDRS AY  R  AKS+V  G C +V
Sbjct:  281 AFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRV 314

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK2_HUMAN (S-adenosylmethionine synthetase isoform type-2 OS=Homo sapiens GN=MAT2A PE=1 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 3.554e-25
Identity = 46/86 (53.49%), Postives = 63/86 (73.26%), Query Frame = 1

Query:  106 IVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKW 363
            +VQVSYAIGV  PLS+ +  YGT +  ++E+L+IVK++FD RPG++  +LDLK+     + +TAAYGHFGRD   F WEV K LK+
Sbjct:  315 LVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKKNFDLRPGVIVRDLDLKK---PIYQRTAAYGHFGRD--SFPWEVPKKLKY 395

HSP 2 Score: 44.669 bits (104), Expect = 3.554e-25
Identity = 22/34 (64.71%), Postives = 24/34 (70.59%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQV 104
            AFSGKD TKVDRS AY  R  AKS+V  G C +V
Sbjct:  281 AFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRV 314

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_CLOPS (S-adenosylmethionine synthetase OS=Clostridium perfringens (strain SM101 / Type A) GN=metK PE=3 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 3.554e-25
Identity = 43/78 (55.13%), Postives = 58/78 (74.36%), Query Frame = 1

Query:  109 VQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            VQV+YAIGV +P+S+ VD++GTGKI D+EI+ I+ + FD RPG +  +LDL+R     + +TAAYGHFGR D D  WE
Sbjct:  305 VQVAYAIGVAKPVSIIVDTFGTGKISDEEIVNIINKVFDLRPGAIIRDLDLRR---PIYRQTAAYGHFGRTDLDLPWE 379

HSP 2 Score: 44.669 bits (104), Expect = 3.554e-25
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRS AY  R  AK++VA G
Sbjct:  270 AFSGKDPTKVDRSAAYAARWVAKNLVAAG 298

The following BLAST results are available for this feature:

BLAST of CK740285 vs. ExPASy Swiss-Prot

BLAST of CK740285 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
METK_BORPA	2.731e-25	56.25	S-adenosylmethionine synthetase OS=Bordetella para...	[more]
METK_BORBR	2.731e-25	56.25	S-adenosylmethionine synthetase OS=Bordetella bron...	[more]
METK_SACD2	2.732e-25	56.25	S-adenosylmethionine synthetase OS=Saccharophagus ...	[more]
METK_CALS8	3.553e-25	56.25	S-adenosylmethionine synthetase OS=Caldicellulosir...	[more]
METK_CLOCE	3.553e-25	51.25	S-adenosylmethionine synthetase OS=Clostridium cel...	[more]
METK2_PONAB	3.554e-25	53.49	S-adenosylmethionine synthetase isoform type-2 OS=...	[more]
METK2_MOUSE	3.554e-25	53.49	S-adenosylmethionine synthetase isoform type-2 OS=...	[more]
METK2_MACFA	3.554e-25	53.49	S-adenosylmethionine synthetase isoform type-2 OS=...	[more]
METK2_HUMAN	3.554e-25	53.49	S-adenosylmethionine synthetase isoform type-2 OS=...	[more]
METK_CLOPS	3.554e-25	55.13	S-adenosylmethionine synthetase OS=Clostridium per...	[more]

Pages

Properties


Property Name	Value
Genbank description	USDA-FP_6428 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE63C12 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK740285 ID=CK740285; Name=CK740285; organism=Citrus sinensis; type=EST; length=563bp
GTGCTTTCTCTGGAAAAGACCCTACTAAGGTGGATAGGAGTGGTGCATAC
ATTGTTAGGCAGGCTGCTAAGAGCATTGTTGCAAACGGGCCTTGCTCGCA
GGTGCATTGTCCAAGTCTCCTATGCCATTGGTGTGCCTGAGCCATTGTCT
GTTTTTGTGGACTCATATGGAACCGGCAAGATTCCTGACAAGGAGATCCT
CAAAATTGTGAAGGAAAGCTTTGATTTCAGGCCAGGAATGATGACCATCA
ATTTGGATCTCAAAAGGGGCGGTAATGGCAGGTTCTTGAAGACTGCTGCC
TATGGACACTTTGGCAGGGATGACCCAGACTTCACTTGGGAAGTTGTGAA
GCCCCTCAAGTGGGAAAAGCCTCAAGCTTAAATTTGAGTTGAAAAGTGAA
AACTATCCCTGCTGTTCAAGTTTGCTTCTATTGGTAGTGGATGAATAATT
TGAATATTATCTTGCTGCTGCTTTGTAATACTAAGCAAATCTCCTGTGTT
CAGAACTCATACATGTTCCCCCATTCAATTCCTTTCTTTTTCTTCTTTCC
CACTTCTTTTTTG