CK740285

Overview
NameCK740285
Unique NameCK740285
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length563
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK1_ORYSJ (S-adenosylmethionine synthetase 1 OS=Oryza sativa subsp. japonica GN=SAM1 PE=2 SV=1)

HSP 1 Score: 183.726 bits (465), Expect = 1.532e-56
Identity = 84/92 (91.30%), Postives = 90/92 (97.83%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 378
            CIVQVSYAIGVPEPLSVFVD+YGTG+IPDKEILKIVKE+FDFRPGM+ INLDLK+GGNGR+LKTAAYGHFGRDDPDFTWEVVKPLKWEKP A
Sbjct:  305 CIVQVSYAIGVPEPLSVFVDTYGTGRIPDKEILKIVKENFDFRPGMIIINLDLKKGGNGRYLKTAAYGHFGRDDPDFTWEVVKPLKWEKPSA 396

HSP 2 Score: 55.8398 bits (133), Expect = 1.532e-56
Identity = 26/29 (89.66%), Postives = 28/29 (96.55%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRSGAY+ RQAAKSIVA+G
Sbjct:  272 AFSGKDPTKVDRSGAYVARQAAKSIVASG 300
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK1_ORYSI (S-adenosylmethionine synthetase 1 OS=Oryza sativa subsp. indica GN=SAM1 PE=2 SV=2)

HSP 1 Score: 183.726 bits (465), Expect = 1.532e-56
Identity = 84/92 (91.30%), Postives = 90/92 (97.83%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 378
            CIVQVSYAIGVPEPLSVFVD+YGTG+IPDKEILKIVKE+FDFRPGM+ INLDLK+GGNGR+LKTAAYGHFGRDDPDFTWEVVKPLKWEKP A
Sbjct:  305 CIVQVSYAIGVPEPLSVFVDTYGTGRIPDKEILKIVKENFDFRPGMIIINLDLKKGGNGRYLKTAAYGHFGRDDPDFTWEVVKPLKWEKPSA 396

HSP 2 Score: 55.8398 bits (133), Expect = 1.532e-56
Identity = 26/29 (89.66%), Postives = 28/29 (96.55%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRSGAY+ RQAAKSIVA+G
Sbjct:  272 AFSGKDPTKVDRSGAYVARQAAKSIVASG 300
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_DENCR (S-adenosylmethionine synthetase OS=Dendrobium crumenatum GN=SAMS PE=2 SV=1)

HSP 1 Score: 180.259 bits (456), Expect = 1.532e-56
Identity = 85/92 (92.39%), Postives = 90/92 (97.83%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 378
            CIVQVSYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKE+FDFRPGM+TINLDLKRGGN RF+KTAAYGHFGRDDPDFTWEVVKPLKW+KP A
Sbjct:  305 CIVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKENFDFRPGMITINLDLKRGGN-RFIKTAAYGHFGRDDPDFTWEVVKPLKWDKPAA 395

HSP 2 Score: 59.3066 bits (142), Expect = 1.532e-56
Identity = 28/29 (96.55%), Postives = 29/29 (100.00%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRSGAYIVRQAAKS+VANG
Sbjct:  272 AFSGKDPTKVDRSGAYIVRQAAKSVVANG 300
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK2_BETVU (S-adenosylmethionine synthetase 2 OS=Beta vulgaris GN=SAMS2 PE=2 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 1.998e-56
Identity = 84/88 (95.45%), Postives = 87/88 (98.86%), Query Frame = 1 
Query:  106 IVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEK 369
            IVQVSYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKE+FDFRPGMM+INLDLKRGGNGRF KTAAYGHFGRDDPDFTWEVVKPLKWEK
Sbjct:  303 IVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKETFDFRPGMMSINLDLKRGGNGRFQKTAAYGHFGRDDPDFTWEVVKPLKWEK 390

HSP 2 Score: 59.6918 bits (143), Expect = 1.998e-56
Identity = 29/29 (100.00%), Postives = 29/29 (100.00%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRSGAYIVRQAAKSIVANG
Sbjct:  269 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 297
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK1_PETCR (S-adenosylmethionine synthetase 1 (Fragment) OS=Petroselinum crispum GN=SMS-1 PE=2 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 2.618e-56
Identity = 83/89 (93.26%), Postives = 89/89 (100.00%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEK 369
            CIVQVSYAIGVPEPLSVFVD+YGTGKIPD+EILKIVKE+FDFRPGM++INLDLKRGGNGRFLKTAAYGHFGR+DPDFTWEVVKPLKWEK
Sbjct:  145 CIVQVSYAIGVPEPLSVFVDTYGTGKIPDREILKIVKETFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGREDPDFTWEVVKPLKWEK 233

HSP 2 Score: 57.7658 bits (138), Expect = 2.618e-56
Identity = 28/29 (96.55%), Postives = 29/29 (100.00%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRSGAYIVRQAAKSIVA+G
Sbjct:  112 AFSGKDPTKVDRSGAYIVRQAAKSIVASG 140
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK2_PETCR (S-adenosylmethionine synthetase 2 (Fragment) OS=Petroselinum crispum GN=SMS-2 PE=2 SV=1)

HSP 1 Score: 180.644 bits (457), Expect = 3.441e-56
Identity = 83/89 (93.26%), Postives = 89/89 (100.00%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEK 369
            CIVQVSYAIGVPEPLSVFVDSYGTGKIPD+EIL+IVKE+FDFRPGM++INLDLKRGGNGRFLKTAAYGHFGR+DPDFTWEVVKPLKWEK
Sbjct:   56 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDREILQIVKETFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGREDPDFTWEVVKPLKWEK 144

HSP 2 Score: 57.7658 bits (138), Expect = 3.441e-56
Identity = 28/29 (96.55%), Postives = 29/29 (100.00%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRSGAYIVRQAAKSIVA+G
Sbjct:   23 AFSGKDPTKVDRSGAYIVRQAAKSIVASG 51
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_GOSHI (S-adenosylmethionine synthetase OS=Gossypium hirsutum GN=SAMS PE=2 SV=1)

HSP 1 Score: 177.178 bits (448), Expect = 9.807e-56
Identity = 82/92 (89.13%), Postives = 87/92 (94.57%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 378
            C+VQVSYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKE+FDFRPGM+ INLDLKRGGN RFLKTAAYGHFGRDD DFTWEVVKPLKW+K  A
Sbjct:  302 CVVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKETFDFRPGMIAINLDLKRGGNSRFLKTAAYGHFGRDDTDFTWEVVKPLKWDKVHA 393

HSP 2 Score: 59.6918 bits (143), Expect = 9.807e-56
Identity = 29/29 (100.00%), Postives = 29/29 (100.00%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRSGAYIVRQAAKSIVANG
Sbjct:  269 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 297
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK4_VITVI (S-adenosylmethionine synthetase 4 OS=Vitis vinifera GN=METK4 PE=3 SV=1)

HSP 1 Score: 178.333 bits (451), Expect = 1.667e-55
Identity = 85/91 (93.41%), Postives = 87/91 (95.60%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQ 375
            CIVQVSYAIGVPEPLSVFVD+Y TGKIPDKEILKIVKESFDFRPGM+ INLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLK EK Q
Sbjct:  302 CIVQVSYAIGVPEPLSVFVDTYATGKIPDKEILKIVKESFDFRPGMIAINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKAEKAQ 392

HSP 2 Score: 57.7658 bits (138), Expect = 1.667e-55
Identity = 28/29 (96.55%), Postives = 29/29 (100.00%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRSGAYIVRQAAKSIVA+G
Sbjct:  269 AFSGKDPTKVDRSGAYIVRQAAKSIVASG 297
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK2_POPTR (S-adenosylmethionine synthetase 2 OS=Populus trichocarpa GN=METK2 PE=2 SV=1)

HSP 1 Score: 178.333 bits (451), Expect = 1.667e-55
Identity = 84/91 (92.31%), Postives = 87/91 (95.60%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQ 375
            CIVQVSYAIGVPEPLSVFVD+YGTGKIPDKEIL IVKE FDFRPGM+ I+LDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLK EKPQ
Sbjct:  302 CIVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILNIVKEKFDFRPGMIAISLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKSEKPQ 392

HSP 2 Score: 57.7658 bits (138), Expect = 1.667e-55
Identity = 28/29 (96.55%), Postives = 29/29 (100.00%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRSGAYIVRQAAKSIVA+G
Sbjct:  269 AFSGKDPTKVDRSGAYIVRQAAKSIVASG 297
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK2_PICSI (S-adenosylmethionine synthetase 2 OS=Picea sitchensis GN=METK2 PE=2 SV=1)

HSP 1 Score: 179.104 bits (453), Expect = 2.173e-55
Identity = 81/92 (88.04%), Postives = 88/92 (95.65%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 378
            C+VQVSYAIGVPEPLSVFVDSYGTG IPDKEIL+++KE+FDFRPGM+TINLDLKRGGNGRF KTAAYGHFGRDDPDFTWE VKPLKW+K QA
Sbjct:  302 CLVQVSYAIGVPEPLSVFVDSYGTGSIPDKEILELIKENFDFRPGMITINLDLKRGGNGRFQKTAAYGHFGRDDPDFTWETVKPLKWDKAQA 393

HSP 2 Score: 56.6102 bits (135), Expect = 2.173e-55
Identity = 28/29 (96.55%), Postives = 28/29 (96.55%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRSGAYIVRQAAKSIVA G
Sbjct:  269 AFSGKDPTKVDRSGAYIVRQAAKSIVAAG 297
The following BLAST results are available for this feature:
BLAST of CK740285 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK1_ORYSJ1.532e-5691.30S-adenosylmethionine synthetase 1 OS=Oryza sativa ... [more]
METK1_ORYSI1.532e-5691.30S-adenosylmethionine synthetase 1 OS=Oryza sativa ... [more]
METK_DENCR1.532e-5692.39S-adenosylmethionine synthetase OS=Dendrobium crum... [more]
METK2_BETVU1.998e-5695.45S-adenosylmethionine synthetase 2 OS=Beta vulgaris... [more]
METK1_PETCR2.618e-5693.26S-adenosylmethionine synthetase 1 (Fragment) OS=Pe... [more]
METK2_PETCR3.441e-5693.26S-adenosylmethionine synthetase 2 (Fragment) OS=Pe... [more]
METK_GOSHI9.807e-5689.13S-adenosylmethionine synthetase OS=Gossypium hirsu... [more]
METK4_VITVI1.667e-5593.41S-adenosylmethionine synthetase 4 OS=Vitis vinifer... [more]
METK2_POPTR1.667e-5592.31S-adenosylmethionine synthetase 2 OS=Populus trich... [more]
METK2_PICSI2.173e-5588.04S-adenosylmethionine synthetase 2 OS=Picea sitchen... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_6428 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE63C12 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK740285 ID=CK740285; Name=CK740285; organism=Citrus sinensis; type=EST; length=563bp
GTGCTTTCTCTGGAAAAGACCCTACTAAGGTGGATAGGAGTGGTGCATAC
ATTGTTAGGCAGGCTGCTAAGAGCATTGTTGCAAACGGGCCTTGCTCGCA
GGTGCATTGTCCAAGTCTCCTATGCCATTGGTGTGCCTGAGCCATTGTCT
GTTTTTGTGGACTCATATGGAACCGGCAAGATTCCTGACAAGGAGATCCT
CAAAATTGTGAAGGAAAGCTTTGATTTCAGGCCAGGAATGATGACCATCA
ATTTGGATCTCAAAAGGGGCGGTAATGGCAGGTTCTTGAAGACTGCTGCC
TATGGACACTTTGGCAGGGATGACCCAGACTTCACTTGGGAAGTTGTGAA
GCCCCTCAAGTGGGAAAAGCCTCAAGCTTAAATTTGAGTTGAAAAGTGAA
AACTATCCCTGCTGTTCAAGTTTGCTTCTATTGGTAGTGGATGAATAATT
TGAATATTATCTTGCTGCTGCTTTGTAATACTAAGCAAATCTCCTGTGTT
CAGAACTCATACATGTTCCCCCATTCAATTCCTTTCTTTTTCTTCTTTCC
CACTTCTTTTTTG
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