CK740285 | Citrus Genome Database

Overview


Name	CK740285
Unique Name	CK740285
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	563

Libraries


Library Name	Type
Ridge pineapple sweet orange entire seedling	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_PSEAE (S-adenosylmethionine synthetase OS=Pseudomonas aeruginosa GN=metK PE=3 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 3.568e-21
Identity = 40/91 (43.96%), Postives = 59/91 (64.84%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFT--WEVVKPLKWEK 369
            C +QVSYAIGV +P S+ ++++GTGK+ D++I+++V+E FD RP  +T  LDL    +  +  TAAYGHFGR   + T   +      WEK
Sbjct:  296 CEIQVSYAIGVAQPTSISINTFGTGKVSDEKIVQLVREHFDLRPYAITKMLDLL---HPMYQPTAAYGHFGRHPFELTVDGDTFTAFTWEK 383

HSP 2 Score: 43.1282 bits (100), Expect = 3.568e-21
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK+IVA G
Sbjct:  263 AFSGKDPSKVDRSAAYAGRYVAKNIVAAG 291

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_PSEAB (S-adenosylmethionine synthetase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=metK PE=3 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 3.568e-21
Identity = 40/91 (43.96%), Postives = 59/91 (64.84%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFT--WEVVKPLKWEK 369
            C +QVSYAIGV +P S+ ++++GTGK+ D++I+++V+E FD RP  +T  LDL    +  +  TAAYGHFGR   + T   +      WEK
Sbjct:  296 CEIQVSYAIGVAQPTSISINTFGTGKVSDEKIVQLVREHFDLRPYAITKMLDLL---HPMYQPTAAYGHFGRHPFELTVDGDTFTAFTWEK 383

HSP 2 Score: 43.1282 bits (100), Expect = 3.568e-21
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK+IVA G
Sbjct:  263 AFSGKDPSKVDRSAAYAGRYVAKNIVAAG 291

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_PSEA8 (S-adenosylmethionine synthetase OS=Pseudomonas aeruginosa (strain LESB58) GN=metK PE=3 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 3.568e-21
Identity = 40/91 (43.96%), Postives = 59/91 (64.84%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFT--WEVVKPLKWEK 369
            C +QVSYAIGV +P S+ ++++GTGK+ D++I+++V+E FD RP  +T  LDL    +  +  TAAYGHFGR   + T   +      WEK
Sbjct:  296 CEIQVSYAIGVAQPTSISINTFGTGKVSDEKIVQLVREHFDLRPYAITKMLDLL---HPMYQPTAAYGHFGRHPFELTVDGDTFTAFTWEK 383

HSP 2 Score: 43.1282 bits (100), Expect = 3.568e-21
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK+IVA G
Sbjct:  263 AFSGKDPSKVDRSAAYAGRYVAKNIVAAG 291

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_PSEA7 (S-adenosylmethionine synthetase OS=Pseudomonas aeruginosa (strain PA7) GN=metK PE=3 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 3.568e-21
Identity = 40/91 (43.96%), Postives = 59/91 (64.84%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFT--WEVVKPLKWEK 369
            C +QVSYAIGV +P S+ ++++GTGK+ D++I+++V+E FD RP  +T  LDL    +  +  TAAYGHFGR   + T   +      WEK
Sbjct:  296 CEIQVSYAIGVAQPTSISINTFGTGKVSDEKIVQLVREHFDLRPYAITKMLDLL---HPMYQPTAAYGHFGRHPFELTVDGDTFTAFTWEK 383

HSP 2 Score: 43.1282 bits (100), Expect = 3.568e-21
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK+IVA G
Sbjct:  263 AFSGKDPSKVDRSAAYAGRYVAKNIVAAG 291

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_THEFY (S-adenosylmethionine synthetase OS=Thermobifida fusca (strain YX) GN=metK PE=3 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 3.568e-21
Identity = 37/78 (47.44%), Postives = 53/78 (67.95%), Query Frame = 1

Query:  109 VQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            VQV+YAIG   P+ VF++++GT K+  +++ K V E FD RP  +  +LDL R     + +T+ YGHFGR+ PDFTWE
Sbjct:  309 VQVAYAIGKAHPVGVFLETFGTEKVAPEQLEKAVLEVFDLRPAAIIRDLDLLR---PIYSQTSVYGHFGRELPDFTWE 383

HSP 2 Score: 44.669 bits (104), Expect = 3.568e-21
Identity = 22/34 (64.71%), Postives = 25/34 (73.53%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQV 104
            AFSGKDP+KVDRS AY  R  AK+IVA G   +V
Sbjct:  274 AFSGKDPSKVDRSAAYATRWVAKNIVAAGLADRV 307

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_LEUMM (S-adenosylmethionine synthetase OS=Leuconostoc mesenteroides subsp. mesenteroides (strain ATCC 8293 / NCDO 523) GN=metK PE=3 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 3.571e-21
Identity = 40/84 (47.62%), Postives = 59/84 (70.24%), Query Frame = 1

Query:  109 VQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLK 360
            +QVSYAIGV  P+S+ VDS+GT K+ +++I +I+ + FDFRP  +  NL+L+R     + +TAA+GHFGR D D  WE +  +K
Sbjct:  299 LQVSYAIGVARPVSLNVDSFGTAKVSEEKINEIITKLFDFRPLAIINNLNLRR---PIYKQTAAFGHFGRTDIDLPWESLDKVK 379

HSP 2 Score: 41.5874 bits (96), Expect = 3.571e-21
Identity = 20/29 (68.97%), Postives = 22/29 (75.86%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKD TKVDRS AY  R  AK++VA G
Sbjct:  264 AFSGKDATKVDRSAAYYARYVAKNMVAAG 292

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_PROMH (S-adenosylmethionine synthetase OS=Proteus mirabilis (strain HI4320) GN=metK PE=3 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 3.571e-21
Identity = 40/80 (50.00%), Postives = 54/80 (67.50%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ V+++GT KIP  +++ +V+E FD RP  +   LDL    +  + KTAAYGHFGR   +F WE
Sbjct:  295 CEIQVSYAIGVAEPTSIMVETFGTEKIPTSQLILLVREFFDLRPYGLIQMLDLL---HPIYQKTAAYGHFGR--AEFPWE 369

HSP 2 Score: 44.2838 bits (103), Expect = 3.571e-21
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK+IVA G
Sbjct:  262 AFSGKDPSKVDRSAAYAARYVAKNIVAAG 290

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_BACSK (S-adenosylmethionine synthetase OS=Bacillus clausii (strain KSM-K16) GN=metK PE=3 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 4.640e-21
Identity = 36/80 (45.00%), Postives = 55/80 (68.75%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C VQ++YAIGV +P+S+ V+++GTGK  +  ++++V++ FD RP  +   LDL+R     + +TAAYGHFGR D    WE
Sbjct:  311 CEVQLAYAIGVAQPVSIAVNTFGTGKAAEDVLVELVRKHFDLRPAGIIRMLDLRR---PIYKQTAAYGHFGRTDVPLPWE 387

HSP 2 Score: 45.0542 bits (105), Expect = 4.640e-21
Identity = 22/29 (75.86%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKD TKVDRSGAY  R  AK+IVA G
Sbjct:  278 AFSGKDATKVDRSGAYAARYVAKNIVAAG 306

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_BACSU (S-adenosylmethionine synthetase OS=Bacillus subtilis GN=metK PE=3 SV=2)

HSP 1 Score: 79.337 bits (194), Expect = 4.641e-21
Identity = 35/80 (43.75%), Postives = 58/80 (72.50%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C VQ++YAIGV +P+S+ ++++G+GK  +++++++V+ +FD RP  +   LDL+R     + +TAAYGHFGR D D  WE
Sbjct:  309 CEVQLAYAIGVAQPVSISINTFGSGKASEEKLIEVVRNNFDLRPAGIIKMLDLRR---PIYKQTAAYGHFGRHDVDLPWE 385

HSP 2 Score: 41.2022 bits (95), Expect = 4.641e-21
Identity = 20/27 (74.07%), Postives = 21/27 (77.78%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVA 83
            AFSGKD TKVDRS AY  R  AK+IVA
Sbjct:  276 AFSGKDATKVDRSAAYAARYVAKNIVA 302

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_STAHJ (S-adenosylmethionine synthetase OS=Staphylococcus haemolyticus (strain JCSC1435) GN=metK PE=3 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 4.642e-21
Identity = 36/80 (45.00%), Postives = 55/80 (68.75%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C VQ++YAIGV EP+S+ +D++ TGK+ + ++++ V+ +FD RP  +   LDLK   +  + +TAAYGHFGR D    WE
Sbjct:  308 CEVQLAYAIGVAEPVSISIDTFKTGKVSEAQLVEAVRANFDLRPAGIIKMLDLK---HPIYKQTAAYGHFGRTDVLLPWE 384

HSP 2 Score: 45.0542 bits (105), Expect = 4.642e-21
Identity = 22/32 (68.75%), Postives = 23/32 (71.88%), Query Frame = 3

Query:    6 FSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQ 101
            FSGKDPTKVDRS AY  R  AK+IVA G   Q
Sbjct:  276 FSGKDPTKVDRSAAYAARYVAKNIVAAGLADQ 307

The following BLAST results are available for this feature:

BLAST of CK740285 vs. ExPASy Swiss-Prot

BLAST of CK740285 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
METK_PSEAE	3.568e-21	43.96	S-adenosylmethionine synthetase OS=Pseudomonas aer...	[more]
METK_PSEAB	3.568e-21	43.96	S-adenosylmethionine synthetase OS=Pseudomonas aer...	[more]
METK_PSEA8	3.568e-21	43.96	S-adenosylmethionine synthetase OS=Pseudomonas aer...	[more]
METK_PSEA7	3.568e-21	43.96	S-adenosylmethionine synthetase OS=Pseudomonas aer...	[more]
METK_THEFY	3.568e-21	47.44	S-adenosylmethionine synthetase OS=Thermobifida fu...	[more]
METK_LEUMM	3.571e-21	47.62	S-adenosylmethionine synthetase OS=Leuconostoc mes...	[more]
METK_PROMH	3.571e-21	50.00	S-adenosylmethionine synthetase OS=Proteus mirabil...	[more]
METK_BACSK	4.640e-21	45.00	S-adenosylmethionine synthetase OS=Bacillus clausi...	[more]
METK_BACSU	4.641e-21	43.75	S-adenosylmethionine synthetase OS=Bacillus subtil...	[more]
METK_STAHJ	4.642e-21	45.00	S-adenosylmethionine synthetase OS=Staphylococcus ...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_6428 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE63C12 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK740285 ID=CK740285; Name=CK740285; organism=Citrus sinensis; type=EST; length=563bp
GTGCTTTCTCTGGAAAAGACCCTACTAAGGTGGATAGGAGTGGTGCATAC
ATTGTTAGGCAGGCTGCTAAGAGCATTGTTGCAAACGGGCCTTGCTCGCA
GGTGCATTGTCCAAGTCTCCTATGCCATTGGTGTGCCTGAGCCATTGTCT
GTTTTTGTGGACTCATATGGAACCGGCAAGATTCCTGACAAGGAGATCCT
CAAAATTGTGAAGGAAAGCTTTGATTTCAGGCCAGGAATGATGACCATCA
ATTTGGATCTCAAAAGGGGCGGTAATGGCAGGTTCTTGAAGACTGCTGCC
TATGGACACTTTGGCAGGGATGACCCAGACTTCACTTGGGAAGTTGTGAA
GCCCCTCAAGTGGGAAAAGCCTCAAGCTTAAATTTGAGTTGAAAAGTGAA
AACTATCCCTGCTGTTCAAGTTTGCTTCTATTGGTAGTGGATGAATAATT
TGAATATTATCTTGCTGCTGCTTTGTAATACTAAGCAAATCTCCTGTGTT
CAGAACTCATACATGTTCCCCCATTCAATTCCTTTCTTTTTCTTCTTTCC
CACTTCTTTTTTG