CK740285 | Citrus Genome Database

Overview


Name	CK740285
Unique Name	CK740285
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	563

Libraries


Library Name	Type
Ridge pineapple sweet orange entire seedling	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_BRAJA (S-adenosylmethionine synthetase OS=Bradyrhizobium japonicum GN=metK PE=3 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 4.642e-21
Identity = 34/83 (40.96%), Postives = 57/83 (68.67%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGR---DDPDFTWE 342
            C +Q++YAIGV  PLS+++D++GTGK+P++++ K   ++ D  P  +  +LDL R     + +T+AYGHFGR   ++  F+WE
Sbjct:  307 CTLQLAYAIGVARPLSIYIDTHGTGKVPEEQLEKAAAQAMDLTPRGIRSHLDLNR---PIYARTSAYGHFGRTPDNEGGFSWE 386

HSP 2 Score: 45.8246 bits (107), Expect = 4.642e-21
Identity = 22/29 (75.86%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRS AY  R  AK+IVA G
Sbjct:  274 AFSGKDPTKVDRSAAYAARYVAKNIVAAG 302

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_LACBA (S-adenosylmethionine synthetase OS=Lactobacillus brevis (strain ATCC 367 / JCM 1170) GN=metK PE=3 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 4.643e-21
Identity = 39/80 (48.75%), Postives = 55/80 (68.75%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C VQ++YAIGV EP+SV+V+++GTG + + ++   V++ FD RP  +   LDLKR     + +TAAYGHFGR D D  WE
Sbjct:  306 CEVQIAYAIGVAEPVSVYVNTFGTGTVAEAKLATAVRDLFDLRPAGIIQMLDLKR---PIYKQTAAYGHFGRTDIDLPWE 382

HSP 2 Score: 39.6614 bits (91), Expect = 4.643e-21
Identity = 19/27 (70.37%), Postives = 21/27 (77.78%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVA 83
            AFSGKD TKVDRS +Y  R  AK+IVA
Sbjct:  273 AFSGKDATKVDRSASYAARYIAKNIVA 299

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_YERE8 (S-adenosylmethionine synthetase OS=Yersinia enterocolitica serotype O:8 / biotype 1B (strain 8081) GN=metK PE=3 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 4.645e-21
Identity = 39/80 (48.75%), Postives = 55/80 (68.75%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ V+++GTGKI +  ++ +V+E F+ RP  +   LDL    +  + KTAAYGHFGR+  +F WE
Sbjct:  295 CEIQVSYAIGVAEPTSIMVETFGTGKIAEDRLVALVREFFELRPYGLIQMLDLL---HPIYRKTAAYGHFGRE--EFPWE 369

HSP 2 Score: 44.2838 bits (103), Expect = 4.645e-21
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK+IVA G
Sbjct:  262 AFSGKDPSKVDRSAAYAARYVAKNIVAAG 290

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_XANCP (S-adenosylmethionine synthetase OS=Xanthomonas campestris pv. campestris GN=metK PE=3 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 6.036e-21
Identity = 40/79 (50.63%), Postives = 54/79 (68.35%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTW 339
            C VQVSYAIGV EP S+ V ++GTGKI D+ I K++++ FD RP  +   LDL    +  + +TA+YGHFGR   DFT+
Sbjct:  297 CEVQVSYAIGVAEPTSISVTTFGTGKIADELIEKLIRKHFDLRPFGIIQMLDLI---HPMYQQTASYGHFGRKPKDFTY 372

HSP 2 Score: 43.8986 bits (102), Expect = 6.036e-21
Identity = 20/29 (68.97%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK++VA G
Sbjct:  264 AFSGKDPSKVDRSAAYAARYVAKNVVAAG 292

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_XANCB (S-adenosylmethionine synthetase OS=Xanthomonas campestris pv. campestris (strain B100) GN=metK PE=3 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 6.036e-21
Identity = 40/79 (50.63%), Postives = 54/79 (68.35%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTW 339
            C VQVSYAIGV EP S+ V ++GTGKI D+ I K++++ FD RP  +   LDL    +  + +TA+YGHFGR   DFT+
Sbjct:  297 CEVQVSYAIGVAEPTSISVTTFGTGKIADELIEKLIRKHFDLRPFGIIQMLDLI---HPMYQQTASYGHFGRKPKDFTY 372

HSP 2 Score: 43.8986 bits (102), Expect = 6.036e-21
Identity = 20/29 (68.97%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK++VA G
Sbjct:  264 AFSGKDPSKVDRSAAYAARYVAKNVVAAG 292

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_XANC8 (S-adenosylmethionine synthetase OS=Xanthomonas campestris pv. campestris (strain 8004) GN=metK PE=3 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 6.036e-21
Identity = 40/79 (50.63%), Postives = 54/79 (68.35%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTW 339
            C VQVSYAIGV EP S+ V ++GTGKI D+ I K++++ FD RP  +   LDL    +  + +TA+YGHFGR   DFT+
Sbjct:  297 CEVQVSYAIGVAEPTSISVTTFGTGKIADELIEKLIRKHFDLRPFGIIQMLDLI---HPMYQQTASYGHFGRKPKDFTY 372

HSP 2 Score: 43.8986 bits (102), Expect = 6.036e-21
Identity = 20/29 (68.97%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK++VA G
Sbjct:  264 AFSGKDPSKVDRSAAYAARYVAKNVVAAG 292

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_XYLFM (S-adenosylmethionine synthetase OS=Xylella fastidiosa (strain M12) GN=metK PE=3 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 6.036e-21
Identity = 41/96 (42.71%), Postives = 58/96 (60.42%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE-------VVKPLKWEK 369
            C VQVSYAIGV EP+S+ V ++GTGK+PD +I K++++ FD RP  +   LDL    +  +  +A+YGHFGR   +F +        V     WEK
Sbjct:  297 CEVQVSYAIGVAEPISISVTTFGTGKVPDDKIEKLIRQHFDLRPYGIIKMLDLI---HPIYQPSASYGHFGRKPREFAYTNSNGGRVVATAFSWEK 389

HSP 2 Score: 44.2838 bits (103), Expect = 6.036e-21
Identity = 20/29 (68.97%), Postives = 24/29 (82.76%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS +Y VR  AK++VA G
Sbjct:  264 AFSGKDPSKVDRSASYAVRYVAKNVVAAG 292

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_CORU7 (S-adenosylmethionine synthetase OS=Corynebacterium urealyticum (strain ATCC 43042 / DSM 7109) GN=metK PE=3 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 6.037e-21
Identity = 38/78 (48.72%), Postives = 52/78 (66.67%), Query Frame = 1

Query:  109 VQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            VQV+YAIGV  P+ ++V+++GT K P ++I + V E FD RP  +   LDL R     + +TAAYGHFGR+D D  WE
Sbjct:  312 VQVAYAIGVARPVGLYVETFGTEKAPVEDIQRAVAEVFDLRPASILRELDLLR---PIYAQTAAYGHFGRNDLDLPWE 386

HSP 2 Score: 43.8986 bits (102), Expect = 6.037e-21
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK+IVA G
Sbjct:  277 AFSGKDPSKVDRSAAYATRWVAKNIVAAG 305

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_NEUCR (S-adenosylmethionine synthetase OS=Neurospora crassa GN=eth-1 PE=2 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 6.039e-21
Identity = 40/86 (46.51%), Postives = 60/86 (69.77%), Query Frame = 1

Query:  106 IVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKW 363
            +VQ+SYAIGV EPLS++VD+YGT     +E+++I++ +FD RPG++   LDL +     +L+TA  GHFG +   F+WE  K LK+
Sbjct:  314 LVQLSYAIGVAEPLSIYVDTYGTSDKTSEELVQIIRNNFDLRPGVIVKELDLAK---PIYLQTAKNGHFGTNQ-SFSWEKPKALKF 395

HSP 2 Score: 40.0466 bits (92), Expect = 6.039e-21
Identity = 20/29 (68.97%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKD +KVDRS AY+ R  AKS+VA G
Sbjct:  280 AFSGKDFSKVDRSAAYVGRWIAKSLVAAG 308

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_DESAP (S-adenosylmethionine synthetase OS=Desulforudis audaxviator (strain MP104C) GN=metK PE=3 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 6.039e-21
Identity = 37/80 (46.25%), Postives = 54/80 (67.50%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C V V+YAIGV +P+++ VD++GTG I ++ + K+V E FD RP  +  +LDL+R     + + AAYGHFGR D +  WE
Sbjct:  304 CEVHVAYAIGVAKPVALRVDTFGTGLINEERLEKLVLEHFDLRPAAIIHHLDLRR---PIYRQVAAYGHFGRGDLNLPWE 380

HSP 2 Score: 45.4394 bits (106), Expect = 6.039e-21
Identity = 22/33 (66.67%), Postives = 25/33 (75.76%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQ 101
            AFSGKDPTKVDRS +Y  R  AK+IVA G  S+
Sbjct:  271 AFSGKDPTKVDRSASYAARYVAKNIVAAGLASR 303

The following BLAST results are available for this feature:

BLAST of CK740285 vs. ExPASy Swiss-Prot

BLAST of CK740285 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
METK_BRAJA	4.642e-21	40.96	S-adenosylmethionine synthetase OS=Bradyrhizobium ...	[more]
METK_LACBA	4.643e-21	48.75	S-adenosylmethionine synthetase OS=Lactobacillus b...	[more]
METK_YERE8	4.645e-21	48.75	S-adenosylmethionine synthetase OS=Yersinia entero...	[more]
METK_XANCP	6.036e-21	50.63	S-adenosylmethionine synthetase OS=Xanthomonas cam...	[more]
METK_XANCB	6.036e-21	50.63	S-adenosylmethionine synthetase OS=Xanthomonas cam...	[more]
METK_XANC8	6.036e-21	50.63	S-adenosylmethionine synthetase OS=Xanthomonas cam...	[more]
METK_XYLFM	6.036e-21	42.71	S-adenosylmethionine synthetase OS=Xylella fastidi...	[more]
METK_CORU7	6.037e-21	48.72	S-adenosylmethionine synthetase OS=Corynebacterium...	[more]
METK_NEUCR	6.039e-21	46.51	S-adenosylmethionine synthetase OS=Neurospora cras...	[more]
METK_DESAP	6.039e-21	46.25	S-adenosylmethionine synthetase OS=Desulforudis au...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_6428 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE63C12 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK740285 ID=CK740285; Name=CK740285; organism=Citrus sinensis; type=EST; length=563bp
GTGCTTTCTCTGGAAAAGACCCTACTAAGGTGGATAGGAGTGGTGCATAC
ATTGTTAGGCAGGCTGCTAAGAGCATTGTTGCAAACGGGCCTTGCTCGCA
GGTGCATTGTCCAAGTCTCCTATGCCATTGGTGTGCCTGAGCCATTGTCT
GTTTTTGTGGACTCATATGGAACCGGCAAGATTCCTGACAAGGAGATCCT
CAAAATTGTGAAGGAAAGCTTTGATTTCAGGCCAGGAATGATGACCATCA
ATTTGGATCTCAAAAGGGGCGGTAATGGCAGGTTCTTGAAGACTGCTGCC
TATGGACACTTTGGCAGGGATGACCCAGACTTCACTTGGGAAGTTGTGAA
GCCCCTCAAGTGGGAAAAGCCTCAAGCTTAAATTTGAGTTGAAAAGTGAA
AACTATCCCTGCTGTTCAAGTTTGCTTCTATTGGTAGTGGATGAATAATT
TGAATATTATCTTGCTGCTGCTTTGTAATACTAAGCAAATCTCCTGTGTT
CAGAACTCATACATGTTCCCCCATTCAATTCCTTTCTTTTTCTTCTTTCC
CACTTCTTTTTTG