CK740285

Overview
NameCK740285
Unique NameCK740285
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length563
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_PSYCK (S-adenosylmethionine synthetase OS=Psychrobacter cryohalolentis (strain K5) GN=metK PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 1.330e-20
Identity = 40/94 (42.55%), Postives = 58/94 (61.70%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPD--FTWEVVKPLKWEKPQA 378
            C VQVSYAIGV EP S+ ++++GT KI + EI+ +++  FD RP  +T  L+L +     + +TA++GHFGR   +  FTWE     K  K  A
Sbjct:  296 CEVQVSYAIGVAEPTSISINTFGTNKISNDEIISLIRTHFDLRPYGITRMLNLLQ---PMYQQTASFGHFGRQGSETAFTWEKTDKAKILKADA 386

HSP 2 Score: 45.8246 bits (107), Expect = 1.330e-20
Identity = 22/29 (75.86%), Postives = 24/29 (82.76%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY VR  AK+IVA G
Sbjct:  263 AFSGKDPSKVDRSAAYAVRYVAKNIVAAG 291
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_HAEPS (S-adenosylmethionine synthetase OS=Haemophilus parasuis serovar 5 (strain SH0165) GN=metK PE=3 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 1.330e-20
Identity = 37/80 (46.25%), Postives = 55/80 (68.75%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +Q+SYAIG+ EP S+ V+++GTGK+ ++  +++V+E FD RP  +   LDL R     + +TAAYGHFGR+   F WE
Sbjct:  295 CEIQLSYAIGIAEPTSIMVETFGTGKVSNEVFVRLVREHFDLRPYGLIKMLDLIR---PIYRETAAYGHFGRE--HFPWE 369

HSP 2 Score: 44.2838 bits (103), Expect = 1.330e-20
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK+IVA G
Sbjct:  262 AFSGKDPSKVDRSAAYAARYVAKNIVAAG 290
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_DEIGD (S-adenosylmethionine synthetase OS=Deinococcus geothermalis (strain DSM 11300) GN=metK PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 1.728e-20
Identity = 37/79 (46.84%), Postives = 53/79 (67.09%), Query Frame = 1 
Query:  106 IVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            +V+V+YAIG   P+S+ VD+YGTG + D+ +  +V+  FD RP  +   LDL+R     + +TAAYGHFGR  P+F WE
Sbjct:  316 LVEVAYAIGRAHPVSLRVDTYGTGTLSDERLADLVRAHFDARPQAIIAELDLQR---PIYAQTAAYGHFGR--PEFPWE 389

HSP 2 Score: 45.4394 bits (106), Expect = 1.728e-20
Identity = 22/29 (75.86%), Postives = 23/29 (79.31%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRS AY  R  AK+IVA G
Sbjct:  282 AFSGKDPTKVDRSAAYYARYIAKNIVAAG 310
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_CORK4 (S-adenosylmethionine synthetase OS=Corynebacterium kroppenstedtii (strain DSM 44385 / CCUG 35717) GN=metK PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.728e-20
Identity = 38/80 (47.50%), Postives = 51/80 (63.75%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C VQV+YAIG   P+ ++V+++GT K P   I + V++ FD RP  +   LDLKR     + +TAAYGHFGR D D  WE
Sbjct:  310 CEVQVAYAIGRANPVGLYVETFGTEKEPISAIQEAVQKVFDLRPAAIIRELDLKR---PIYAQTAAYGHFGRTDLDLPWE 386

HSP 2 Score: 44.2838 bits (103), Expect = 1.728e-20
Identity = 21/29 (72.41%), Postives = 24/29 (82.76%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY +R  AK+IVA G
Sbjct:  277 AFSGKDPSKVDRSAAYAMRWVAKNIVAAG 305
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_SHISS (S-adenosylmethionine synthetase OS=Shigella sonnei (strain Ss046) GN=metK PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.730e-20
Identity = 38/80 (47.50%), Postives = 54/80 (67.50%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  295 CEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369

HSP 2 Score: 44.2838 bits (103), Expect = 1.730e-20
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK+IVA G
Sbjct:  262 AFSGKDPSKVDRSAAYAARYVAKNIVAAG 290
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_SHIFL (S-adenosylmethionine synthetase OS=Shigella flexneri GN=metK PE=3 SV=2)

HSP 1 Score: 74.3294 bits (181), Expect = 1.730e-20
Identity = 38/80 (47.50%), Postives = 54/80 (67.50%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  295 CEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369

HSP 2 Score: 44.2838 bits (103), Expect = 1.730e-20
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK+IVA G
Sbjct:  262 AFSGKDPSKVDRSAAYAARYVAKNIVAAG 290
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_SHIF8 (S-adenosylmethionine synthetase OS=Shigella flexneri serotype 5b (strain 8401) GN=metK PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.730e-20
Identity = 38/80 (47.50%), Postives = 54/80 (67.50%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  295 CEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369

HSP 2 Score: 44.2838 bits (103), Expect = 1.730e-20
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK+IVA G
Sbjct:  262 AFSGKDPSKVDRSAAYAARYVAKNIVAAG 290
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_SHIDS (S-adenosylmethionine synthetase OS=Shigella dysenteriae serotype 1 (strain Sd197) GN=metK PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.730e-20
Identity = 38/80 (47.50%), Postives = 54/80 (67.50%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  295 CEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369

HSP 2 Score: 44.2838 bits (103), Expect = 1.730e-20
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK+IVA G
Sbjct:  262 AFSGKDPSKVDRSAAYAARYVAKNIVAAG 290
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_SHIBS (S-adenosylmethionine synthetase OS=Shigella boydii serotype 4 (strain Sb227) GN=metK PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.730e-20
Identity = 38/80 (47.50%), Postives = 54/80 (67.50%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  295 CEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369

HSP 2 Score: 44.2838 bits (103), Expect = 1.730e-20
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK+IVA G
Sbjct:  262 AFSGKDPSKVDRSAAYAARYVAKNIVAAG 290
[Close]
BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_SHIB3 (S-adenosylmethionine synthetase OS=Shigella boydii serotype 18 (strain CDC 3083-94 / BS512) GN=metK PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.730e-20
Identity = 38/80 (47.50%), Postives = 54/80 (67.50%), Query Frame = 1 
Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  295 CEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369

HSP 2 Score: 44.2838 bits (103), Expect = 1.730e-20
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3 
Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK+IVA G
Sbjct:  262 AFSGKDPSKVDRSAAYAARYVAKNIVAAG 290
The following BLAST results are available for this feature:
BLAST of CK740285 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK_PSYCK1.330e-2042.55S-adenosylmethionine synthetase OS=Psychrobacter c... [more]
METK_HAEPS1.330e-2046.25S-adenosylmethionine synthetase OS=Haemophilus par... [more]
METK_DEIGD1.728e-2046.84S-adenosylmethionine synthetase OS=Deinococcus geo... [more]
METK_CORK41.728e-2047.50S-adenosylmethionine synthetase OS=Corynebacterium... [more]
METK_SHISS1.730e-2047.50S-adenosylmethionine synthetase OS=Shigella sonnei... [more]
METK_SHIFL1.730e-2047.50S-adenosylmethionine synthetase OS=Shigella flexne... [more]
METK_SHIF81.730e-2047.50S-adenosylmethionine synthetase OS=Shigella flexne... [more]
METK_SHIDS1.730e-2047.50S-adenosylmethionine synthetase OS=Shigella dysent... [more]
METK_SHIBS1.730e-2047.50S-adenosylmethionine synthetase OS=Shigella boydii... [more]
METK_SHIB31.730e-2047.50S-adenosylmethionine synthetase OS=Shigella boydii... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_6428 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE63C12 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK740285 ID=CK740285; Name=CK740285; organism=Citrus sinensis; type=EST; length=563bp
GTGCTTTCTCTGGAAAAGACCCTACTAAGGTGGATAGGAGTGGTGCATAC
ATTGTTAGGCAGGCTGCTAAGAGCATTGTTGCAAACGGGCCTTGCTCGCA
GGTGCATTGTCCAAGTCTCCTATGCCATTGGTGTGCCTGAGCCATTGTCT
GTTTTTGTGGACTCATATGGAACCGGCAAGATTCCTGACAAGGAGATCCT
CAAAATTGTGAAGGAAAGCTTTGATTTCAGGCCAGGAATGATGACCATCA
ATTTGGATCTCAAAAGGGGCGGTAATGGCAGGTTCTTGAAGACTGCTGCC
TATGGACACTTTGGCAGGGATGACCCAGACTTCACTTGGGAAGTTGTGAA
GCCCCTCAAGTGGGAAAAGCCTCAAGCTTAAATTTGAGTTGAAAAGTGAA
AACTATCCCTGCTGTTCAAGTTTGCTTCTATTGGTAGTGGATGAATAATT
TGAATATTATCTTGCTGCTGCTTTGTAATACTAAGCAAATCTCCTGTGTT
CAGAACTCATACATGTTCCCCCATTCAATTCCTTTCTTTTTCTTCTTTCC
CACTTCTTTTTTG
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