CK740285 | Citrus Genome Database

Overview


Name	CK740285
Unique Name	CK740285
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	563

Libraries


Library Name	Type
Ridge pineapple sweet orange entire seedling	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_ECOHS (S-adenosylmethionine synthetase OS=Escherichia coli O9:H4 (strain HS) GN=metK PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.730e-20
Identity = 38/80 (47.50%), Postives = 54/80 (67.50%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  295 CEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369

HSP 2 Score: 44.2838 bits (103), Expect = 1.730e-20
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK+IVA G
Sbjct:  262 AFSGKDPSKVDRSAAYAARYVAKNIVAAG 290

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_ECODH (S-adenosylmethionine synthetase OS=Escherichia coli (strain K12 / DH10B) GN=metK PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.730e-20
Identity = 38/80 (47.50%), Postives = 54/80 (67.50%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  295 CEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369

HSP 2 Score: 44.2838 bits (103), Expect = 1.730e-20
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK+IVA G
Sbjct:  262 AFSGKDPSKVDRSAAYAARYVAKNIVAAG 290

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_ECOBW (S-adenosylmethionine synthetase OS=Escherichia coli (strain K12 / BW2952) GN=metK PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.730e-20
Identity = 38/80 (47.50%), Postives = 54/80 (67.50%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  295 CEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369

HSP 2 Score: 44.2838 bits (103), Expect = 1.730e-20
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK+IVA G
Sbjct:  262 AFSGKDPSKVDRSAAYAARYVAKNIVAAG 290

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_ECO8A (S-adenosylmethionine synthetase OS=Escherichia coli O8 (strain IAI1) GN=metK PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.730e-20
Identity = 38/80 (47.50%), Postives = 54/80 (67.50%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  295 CEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369

HSP 2 Score: 44.2838 bits (103), Expect = 1.730e-20
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK+IVA G
Sbjct:  262 AFSGKDPSKVDRSAAYAARYVAKNIVAAG 290

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_ECO7I (S-adenosylmethionine synthetase OS=Escherichia coli O7:K1 (strain IAI39 / ExPEC) GN=metK PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.730e-20
Identity = 38/80 (47.50%), Postives = 54/80 (67.50%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  295 CEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369

HSP 2 Score: 44.2838 bits (103), Expect = 1.730e-20
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK+IVA G
Sbjct:  262 AFSGKDPSKVDRSAAYAARYVAKNIVAAG 290

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_ECO5E (S-adenosylmethionine synthetase OS=Escherichia coli O157:H7 (strain EC4115 / EHEC) GN=metK PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.730e-20
Identity = 38/80 (47.50%), Postives = 54/80 (67.50%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  295 CEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369

HSP 2 Score: 44.2838 bits (103), Expect = 1.730e-20
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK+IVA G
Sbjct:  262 AFSGKDPSKVDRSAAYAARYVAKNIVAAG 290

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_ECO57 (S-adenosylmethionine synthetase OS=Escherichia coli O157:H7 GN=metK PE=3 SV=2)

HSP 1 Score: 74.3294 bits (181), Expect = 1.730e-20
Identity = 38/80 (47.50%), Postives = 54/80 (67.50%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  295 CEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369

HSP 2 Score: 44.2838 bits (103), Expect = 1.730e-20
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK+IVA G
Sbjct:  262 AFSGKDPSKVDRSAAYAARYVAKNIVAAG 290

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_ECO55 (S-adenosylmethionine synthetase OS=Escherichia coli (strain 55989 / EAEC) GN=metK PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.730e-20
Identity = 38/80 (47.50%), Postives = 54/80 (67.50%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  295 CEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369

HSP 2 Score: 44.2838 bits (103), Expect = 1.730e-20
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK+IVA G
Sbjct:  262 AFSGKDPSKVDRSAAYAARYVAKNIVAAG 290

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_ECO27 (S-adenosylmethionine synthetase OS=Escherichia coli O127:H6 (strain E2348/69 / EPEC) GN=metK PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.730e-20
Identity = 38/80 (47.50%), Postives = 54/80 (67.50%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  295 CEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369

HSP 2 Score: 44.2838 bits (103), Expect = 1.730e-20
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK+IVA G
Sbjct:  262 AFSGKDPSKVDRSAAYAARYVAKNIVAAG 290

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_ECO24 (S-adenosylmethionine synthetase OS=Escherichia coli O139:H28 (strain E24377A / ETEC) GN=metK PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.730e-20
Identity = 38/80 (47.50%), Postives = 54/80 (67.50%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  295 CEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369

HSP 2 Score: 44.2838 bits (103), Expect = 1.730e-20
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK+IVA G
Sbjct:  262 AFSGKDPSKVDRSAAYAARYVAKNIVAAG 290

The following BLAST results are available for this feature:

BLAST of CK740285 vs. ExPASy Swiss-Prot

BLAST of CK740285 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
METK_ECOHS	1.730e-20	47.50	S-adenosylmethionine synthetase OS=Escherichia col...	[more]
METK_ECODH	1.730e-20	47.50	S-adenosylmethionine synthetase OS=Escherichia col...	[more]
METK_ECOBW	1.730e-20	47.50	S-adenosylmethionine synthetase OS=Escherichia col...	[more]
METK_ECO8A	1.730e-20	47.50	S-adenosylmethionine synthetase OS=Escherichia col...	[more]
METK_ECO7I	1.730e-20	47.50	S-adenosylmethionine synthetase OS=Escherichia col...	[more]
METK_ECO5E	1.730e-20	47.50	S-adenosylmethionine synthetase OS=Escherichia col...	[more]
METK_ECO57	1.730e-20	47.50	S-adenosylmethionine synthetase OS=Escherichia col...	[more]
METK_ECO55	1.730e-20	47.50	S-adenosylmethionine synthetase OS=Escherichia col...	[more]
METK_ECO27	1.730e-20	47.50	S-adenosylmethionine synthetase OS=Escherichia col...	[more]
METK_ECO24	1.730e-20	47.50	S-adenosylmethionine synthetase OS=Escherichia col...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_6428 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE63C12 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK740285 ID=CK740285; Name=CK740285; organism=Citrus sinensis; type=EST; length=563bp
GTGCTTTCTCTGGAAAAGACCCTACTAAGGTGGATAGGAGTGGTGCATAC
ATTGTTAGGCAGGCTGCTAAGAGCATTGTTGCAAACGGGCCTTGCTCGCA
GGTGCATTGTCCAAGTCTCCTATGCCATTGGTGTGCCTGAGCCATTGTCT
GTTTTTGTGGACTCATATGGAACCGGCAAGATTCCTGACAAGGAGATCCT
CAAAATTGTGAAGGAAAGCTTTGATTTCAGGCCAGGAATGATGACCATCA
ATTTGGATCTCAAAAGGGGCGGTAATGGCAGGTTCTTGAAGACTGCTGCC
TATGGACACTTTGGCAGGGATGACCCAGACTTCACTTGGGAAGTTGTGAA
GCCCCTCAAGTGGGAAAAGCCTCAAGCTTAAATTTGAGTTGAAAAGTGAA
AACTATCCCTGCTGTTCAAGTTTGCTTCTATTGGTAGTGGATGAATAATT
TGAATATTATCTTGCTGCTGCTTTGTAATACTAAGCAAATCTCCTGTGTT
CAGAACTCATACATGTTCCCCCATTCAATTCCTTTCTTTTTCTTCTTTCC
CACTTCTTTTTTG