CK740285 | Citrus Genome Database

Overview


Name	CK740285
Unique Name	CK740285
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	563

Libraries


Library Name	Type
Ridge pineapple sweet orange entire seedling	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig1234	contig	Csv1_Contig1234:1050..1610.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_CITK8 (S-adenosylmethionine synthetase OS=Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) GN=metK PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.730e-20
Identity = 38/80 (47.50%), Postives = 54/80 (67.50%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  295 CEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369

HSP 2 Score: 44.2838 bits (103), Expect = 1.730e-20
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK+IVA G
Sbjct:  262 AFSGKDPSKVDRSAAYAARYVAKNIVAAG 290

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_SORC5 (S-adenosylmethionine synthetase OS=Sorangium cellulosum (strain So ce56) GN=metK PE=3 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 2.246e-20
Identity = 37/80 (46.25%), Postives = 55/80 (68.75%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C VQ++YAIGV +P+ V V+++GTG++ +  + K + ++FD RP  +   LDL +     + KTAAYGHFGRD  +FTWE
Sbjct:  297 CEVQIAYAIGVAQPVGVHVNTFGTGRVGEDVLEKYIMQNFDMRPKAIIEQLDLLK---PIYRKTAAYGHFGRD--EFTWE 371

HSP 2 Score: 42.743 bits (99), Expect = 2.246e-20
Identity = 19/28 (67.86%), Postives = 22/28 (78.57%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVAN 86
            AFSGKDPTKVDRS  Y  R  AK++VA+
Sbjct:  264 AFSGKDPTKVDRSACYYARYVAKNVVAS 291

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_CORJK (S-adenosylmethionine synthetase OS=Corynebacterium jeikeium (strain K411) GN=metK PE=3 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 2.247e-20
Identity = 36/78 (46.15%), Postives = 50/78 (64.10%), Query Frame = 1

Query:  109 VQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            VQV+YAIG   P+ ++V+++GT K P + I   V++ FD RP  +   LDLKR     + +T+AYGHFGR D D  WE
Sbjct:  318 VQVAYAIGRATPVGLYVETFGTEKAPVEAIQAAVRQVFDLRPAAILQELDLKR---PIYAQTSAYGHFGRTDLDLPWE 392

HSP 2 Score: 46.595 bits (109), Expect = 2.247e-20
Identity = 22/29 (75.86%), Postives = 25/29 (86.21%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRSGAY +R  AK+IVA G
Sbjct:  283 AFSGKDPSKVDRSGAYAMRWVAKNIVAAG 311

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_STAES (S-adenosylmethionine synthetase OS=Staphylococcus epidermidis (strain ATCC 12228) GN=metK PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 2.248e-20
Identity = 35/80 (43.75%), Postives = 54/80 (67.50%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C VQ++YAIGV EP+S+ ++++ TGK+ +  +++ V++ FD RP  +   LDLK+     + +TAAYGHFGR D    WE
Sbjct:  308 CEVQLAYAIGVAEPVSISINTFDTGKVSEARLVEAVRKHFDLRPAGIIKMLDLKQ---PIYRQTAAYGHFGRTDVLLPWE 384

HSP 2 Score: 45.0542 bits (105), Expect = 2.248e-20
Identity = 22/32 (68.75%), Postives = 23/32 (71.88%), Query Frame = 3

Query:    6 FSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQ 101
            FSGKDPTKVDRS AY  R  AK+IVA G   Q
Sbjct:  276 FSGKDPTKVDRSAAYAARYVAKNIVAAGLAKQ 307

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_STAEQ (S-adenosylmethionine synthetase OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) GN=metK PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 2.248e-20
Identity = 35/80 (43.75%), Postives = 54/80 (67.50%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C VQ++YAIGV EP+S+ ++++ TGK+ +  +++ V++ FD RP  +   LDLK+     + +TAAYGHFGR D    WE
Sbjct:  308 CEVQLAYAIGVAEPVSISINTFDTGKVSEARLVEAVRKHFDLRPAGIIKMLDLKQ---PIYRQTAAYGHFGRTDVLLPWE 384

HSP 2 Score: 45.0542 bits (105), Expect = 2.248e-20
Identity = 22/32 (68.75%), Postives = 23/32 (71.88%), Query Frame = 3

Query:    6 FSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQ 101
            FSGKDPTKVDRS AY  R  AK+IVA G   Q
Sbjct:  276 FSGKDPTKVDRSAAYAARYVAKNIVAAGLAKQ 307

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_PSEU5 (S-adenosylmethionine synthetase OS=Pseudomonas stutzeri (strain A1501) GN=metK PE=3 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 2.248e-20
Identity = 40/91 (43.96%), Postives = 57/91 (62.64%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFT--WEVVKPLKWEK 369
            C +QVSYAIGV +P S+ ++++GTGK+ D +I+ +V+E FD RP  +T  LDL    +  +  TAAYGHFGR   + T   +      WEK
Sbjct:  296 CEIQVSYAIGVAQPTSISLNTFGTGKLGDDKIIALVREHFDLRPYAITRMLDLL---HPMYRATAAYGHFGRTPYEMTVGADTFTAFTWEK 383

HSP 2 Score: 43.1282 bits (100), Expect = 2.248e-20
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK+IVA G
Sbjct:  263 AFSGKDPSKVDRSAAYAGRYVAKNIVAAG 291

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_ENTFA (S-adenosylmethionine synthetase OS=Enterococcus faecalis GN=metK PE=3 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 2.249e-20
Identity = 34/78 (43.59%), Postives = 56/78 (71.79%), Query Frame = 1

Query:  109 VQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            VQ++YAIGV +P+S+ ++++GT  +P+ ++++ V+++FD RP  +   LDL+R     + +TAAYGHFGR D D  WE
Sbjct:  306 VQLAYAIGVAQPVSISINTFGTSDLPESKLIEAVRKNFDLRPAGIIEMLDLRR---PIYKQTAAYGHFGRTDIDLPWE 380

HSP 2 Score: 41.9726 bits (97), Expect = 2.249e-20
Identity = 21/34 (61.76%), Postives = 24/34 (70.59%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQV 104
            AFSGKD TKVDRS +Y  R  AK+IVA G   +V
Sbjct:  271 AFSGKDATKVDRSASYAARYIAKNIVAAGLAKKV 304

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_KOSOT (S-adenosylmethionine synthetase OS=Kosmotoga olearia (strain TBF 19.5.1) GN=metK PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 2.249e-20
Identity = 35/78 (44.87%), Postives = 53/78 (67.95%), Query Frame = 1

Query:  109 VQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            +Q++YAIGV  P+S  +D++GT K+  +++ K+VKE FDFRP  +   L+L+R     + + AAYGHFGR D +  WE
Sbjct:  306 LQIAYAIGVAHPVSFMIDAHGTEKVALEKLEKVVKEVFDFRPAAIIDKLNLRR---PIYKQVAAYGHFGRIDVEVPWE 380

HSP 2 Score: 45.0542 bits (105), Expect = 2.249e-20
Identity = 22/34 (64.71%), Postives = 25/34 (73.53%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQV 104
            AFSGKDPTKVDRS  Y+ R  AK+IVA G   +V
Sbjct:  271 AFSGKDPTKVDRSAHYMARYVAKNIVAAGLADRV 304

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_ACTPJ (S-adenosylmethionine synthetase OS=Actinobacillus pleuropneumoniae serotype 3 (strain JL03) GN=metK PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 2.250e-20
Identity = 36/80 (45.00%), Postives = 56/80 (70.00%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +Q+SYAIGV +P S+ V+++GTGK+ ++ ++K++ ++FD RP  +   LDL R     + +TAAYGHFGR+   F WE
Sbjct:  295 CEIQLSYAIGVADPTSIMVETFGTGKVSNETLVKLIYQNFDLRPYGLIKMLDLIR---PIYRETAAYGHFGRE--HFPWE 369

HSP 2 Score: 44.2838 bits (103), Expect = 2.250e-20
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK+IVA G
Sbjct:  262 AFSGKDPSKVDRSAAYAARYVAKNIVAAG 290

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_ACTP7 (S-adenosylmethionine synthetase OS=Actinobacillus pleuropneumoniae serotype 7 (strain AP76) GN=metK PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 2.250e-20
Identity = 36/80 (45.00%), Postives = 56/80 (70.00%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +Q+SYAIGV +P S+ V+++GTGK+ ++ ++K++ ++FD RP  +   LDL R     + +TAAYGHFGR+   F WE
Sbjct:  295 CEIQLSYAIGVADPTSIMVETFGTGKVSNETLVKLIYQNFDLRPYGLIKMLDLIR---PIYRETAAYGHFGRE--HFPWE 369

HSP 2 Score: 44.2838 bits (103), Expect = 2.250e-20
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK+IVA G
Sbjct:  262 AFSGKDPSKVDRSAAYAARYVAKNIVAAG 290

The following BLAST results are available for this feature:

BLAST of CK740285 vs. ExPASy Swiss-Prot

BLAST of CK740285 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
METK_CITK8	1.730e-20	47.50	S-adenosylmethionine synthetase OS=Citrobacter kos...	[more]
METK_SORC5	2.246e-20	46.25	S-adenosylmethionine synthetase OS=Sorangium cellu...	[more]
METK_CORJK	2.247e-20	46.15	S-adenosylmethionine synthetase OS=Corynebacterium...	[more]
METK_STAES	2.248e-20	43.75	S-adenosylmethionine synthetase OS=Staphylococcus ...	[more]
METK_STAEQ	2.248e-20	43.75	S-adenosylmethionine synthetase OS=Staphylococcus ...	[more]
METK_PSEU5	2.248e-20	43.96	S-adenosylmethionine synthetase OS=Pseudomonas stu...	[more]
METK_ENTFA	2.249e-20	43.59	S-adenosylmethionine synthetase OS=Enterococcus fa...	[more]
METK_KOSOT	2.249e-20	44.87	S-adenosylmethionine synthetase OS=Kosmotoga olear...	[more]
METK_ACTPJ	2.250e-20	45.00	S-adenosylmethionine synthetase OS=Actinobacillus ...	[more]
METK_ACTP7	2.250e-20	45.00	S-adenosylmethionine synthetase OS=Actinobacillus ...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_6428 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE63C12 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK740285 ID=CK740285; Name=CK740285; organism=Citrus sinensis; type=EST; length=563bp
GTGCTTTCTCTGGAAAAGACCCTACTAAGGTGGATAGGAGTGGTGCATAC
ATTGTTAGGCAGGCTGCTAAGAGCATTGTTGCAAACGGGCCTTGCTCGCA
GGTGCATTGTCCAAGTCTCCTATGCCATTGGTGTGCCTGAGCCATTGTCT
GTTTTTGTGGACTCATATGGAACCGGCAAGATTCCTGACAAGGAGATCCT
CAAAATTGTGAAGGAAAGCTTTGATTTCAGGCCAGGAATGATGACCATCA
ATTTGGATCTCAAAAGGGGCGGTAATGGCAGGTTCTTGAAGACTGCTGCC
TATGGACACTTTGGCAGGGATGACCCAGACTTCACTTGGGAAGTTGTGAA
GCCCCTCAAGTGGGAAAAGCCTCAAGCTTAAATTTGAGTTGAAAAGTGAA
AACTATCCCTGCTGTTCAAGTTTGCTTCTATTGGTAGTGGATGAATAATT
TGAATATTATCTTGCTGCTGCTTTGTAATACTAAGCAAATCTCCTGTGTT
CAGAACTCATACATGTTCCCCCATTCAATTCCTTTCTTTTTCTTCTTTCC
CACTTCTTTTTTG