CK740285 | Citrus Genome Database

Overview


Name	CK740285
Unique Name	CK740285
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	563

Libraries


Library Name	Type
Ridge pineapple sweet orange entire seedling	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig1234	contig	Csv1_Contig1234:1050..1610.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK2_ATRNU (S-adenosylmethionine synthetase 2 OS=Atriplex nummularia GN=SAMS2 PE=1 SV=1)

HSP 1 Score: 170.629 bits (431), Expect = 2.142e-52
Identity = 80/92 (86.96%), Postives = 87/92 (94.57%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 378
            CIVQ+SYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKE+FDFRPGM+ INLDL +GG+ R+LKTAAYGHFGRDD DFTWE VKPLKWEKPQA
Sbjct:  306 CIVQISYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKETFDFRPGMIAINLDLLKGGS-RYLKTAAYGHFGRDDADFTWETVKPLKWEKPQA 396

HSP 2 Score: 55.0694 bits (131), Expect = 2.142e-52
Identity = 27/29 (93.10%), Postives = 27/29 (93.10%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRSGAYI RQAAKSIVA G
Sbjct:  273 AFSGKDPTKVDRSGAYIARQAAKSIVAAG 301

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK1_ATRNU (S-adenosylmethionine synthetase 1 OS=Atriplex nummularia GN=SAMS1 PE=1 SV=2)

HSP 1 Score: 170.629 bits (431), Expect = 2.142e-52
Identity = 80/92 (86.96%), Postives = 87/92 (94.57%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 378
            CIVQ+SYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKE+FDFRPGM+ INLDL +GG+ R+LKTAAYGHFGRDD DFTWE VKPLKWEKPQA
Sbjct:  306 CIVQISYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKETFDFRPGMIAINLDLLKGGS-RYLKTAAYGHFGRDDADFTWETVKPLKWEKPQA 396

HSP 2 Score: 55.0694 bits (131), Expect = 2.142e-52
Identity = 27/29 (93.10%), Postives = 27/29 (93.10%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRSGAYI RQAAKSIVA G
Sbjct:  273 AFSGKDPTKVDRSGAYIARQAAKSIVAAG 301

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK3_PICSI (S-adenosylmethionine synthetase 3 OS=Picea sitchensis GN=METK3 PE=2 SV=1)

HSP 1 Score: 168.703 bits (426), Expect = 2.793e-52
Identity = 77/89 (86.52%), Postives = 85/89 (95.51%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEK 369
            C+VQVSYAIGVPEPLSVFVDSYGTG IPDK IL+++K++FDFRPGM+TINLDLKRGGNGRF KTAAYGHFGRDDPDFTWE+VKPLK EK
Sbjct:  302 CLVQVSYAIGVPEPLSVFVDSYGTGTIPDKVILELIKKNFDFRPGMITINLDLKRGGNGRFQKTAAYGHFGRDDPDFTWEIVKPLKQEK 390

HSP 2 Score: 56.6102 bits (135), Expect = 2.793e-52
Identity = 28/29 (96.55%), Postives = 28/29 (96.55%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRSGAYIVRQAAKSIVA G
Sbjct:  269 AFSGKDPTKVDRSGAYIVRQAAKSIVAAG 297

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK1_DAUCA (S-adenosylmethionine synthetase 1 OS=Daucus carota GN=SAMS1 PE=2 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 3.636e-52
Identity = 88/140 (62.86%), Postives = 92/140 (65.71%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEIL------------------------------------------------KIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 378
            CIVQVSYAIGVPEPLSVFVDSYGTGKIPD+EIL                                                KIVKE+FDFRPGM++INLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA
Sbjct:  302 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDREILQIVKETFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGTGKIPDREILKIVKETFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 441

HSP 2 Score: 57.7658 bits (138), Expect = 3.636e-52
Identity = 28/29 (96.55%), Postives = 29/29 (100.00%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRSGAYIVRQAAKSIVA+G
Sbjct:  269 AFSGKDPTKVDRSGAYIVRQAAKSIVASG 297

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK1_HORVU (S-adenosylmethionine synthetase 1 OS=Hordeum vulgare GN=SAM1 PE=2 SV=1)

HSP 1 Score: 168.318 bits (425), Expect = 6.185e-52
Identity = 79/92 (85.87%), Postives = 88/92 (95.65%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 378
            CIVQ+SYAIGVPEPLSVFVDSYGTGKIPD+EILK+VKE+FDFRPGM+TINLDLK+GGN RF+KTAAYGHFGRDD DFTWEVVKPLK++K  A
Sbjct:  304 CIVQISYAIGVPEPLSVFVDSYGTGKIPDREILKLVKENFDFRPGMITINLDLKKGGN-RFIKTAAYGHFGRDDADFTWEVVKPLKFDKASA 394

HSP 2 Score: 55.8398 bits (133), Expect = 6.185e-52
Identity = 26/29 (89.66%), Postives = 28/29 (96.55%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRSGAYI RQAAKSI+A+G
Sbjct:  271 AFSGKDPTKVDRSGAYIARQAAKSIIASG 299

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK1_PEA (S-adenosylmethionine synthetase 1 (Fragment) OS=Pisum sativum GN=SAMS1 PE=2 SV=2)

HSP 1 Score: 167.933 bits (424), Expect = 8.069e-52
Identity = 78/88 (88.64%), Postives = 85/88 (96.59%), Query Frame = 1

Query:  106 IVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEK 369
            IVQ+  AIGVPEPLSVFVD+YGTGKIPD+EILKIVKE+FDFRPGM++INLDL RGGNGRFLKTAAYGHFGR+DPDFTWEVVKPLKWEK
Sbjct:  278 IVQLLRAIGVPEPLSVFVDTYGTGKIPDREILKIVKETFDFRPGMISINLDLLRGGNGRFLKTAAYGHFGREDPDFTWEVVKPLKWEK 365

HSP 2 Score: 55.8398 bits (133), Expect = 8.069e-52
Identity = 27/29 (93.10%), Postives = 28/29 (96.55%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDR GAYIVRQAAKSIVA+G
Sbjct:  244 AFSGKDPTKVDRRGAYIVRQAAKSIVASG 272

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK1_TRIMO (S-adenosylmethionine synthetase 1 OS=Triticum monococcum GN=SAMS1 PE=2 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 1.370e-51
Identity = 79/92 (85.87%), Postives = 88/92 (95.65%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 378
            CIVQ+SYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKE+FDFRPGM++INLDLK+GGN RF+KTAAYGHFGR+D DFTWEVVKPLK++K  A
Sbjct:  304 CIVQISYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKKGGN-RFIKTAAYGHFGREDADFTWEVVKPLKFDKASA 394

HSP 2 Score: 55.8398 bits (133), Expect = 1.370e-51
Identity = 26/29 (89.66%), Postives = 28/29 (96.55%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRSGAYI RQAAKSI+A+G
Sbjct:  271 AFSGKDPTKVDRSGAYIARQAAKSIIASG 299

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK3_HORVU (S-adenosylmethionine synthetase 3 OS=Hordeum vulgare GN=SAM3 PE=2 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 1.786e-51
Identity = 78/92 (84.78%), Postives = 88/92 (95.65%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 378
            CIVQ+SYAIGVPEPLSVFVDSYGTGKIPD+EILK+VKE+FDFRPGM++INLDLK+GGN RF+KTAAYGHFGRDD DFTWEVVKPLK++K  A
Sbjct:  304 CIVQISYAIGVPEPLSVFVDSYGTGKIPDREILKLVKENFDFRPGMISINLDLKKGGN-RFIKTAAYGHFGRDDADFTWEVVKPLKFDKASA 394

HSP 2 Score: 55.8398 bits (133), Expect = 1.786e-51
Identity = 26/29 (89.66%), Postives = 28/29 (96.55%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRSGAYI RQAAKSI+A+G
Sbjct:  271 AFSGKDPTKVDRSGAYIARQAAKSIIASG 299

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_CAMSI (S-adenosylmethionine synthetase OS=Camellia sinensis GN=SAM PE=2 SV=1)

HSP 1 Score: 162.925 bits (411), Expect = 1.786e-51
Identity = 76/80 (95.00%), Postives = 78/80 (97.50%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            CIVQVSYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKESFDFRPGM+ INLDLKRGGN RFLKTAAYGHFGRDDPDFTWE
Sbjct:  302 CIVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKESFDFRPGMIAINLDLKRGGNSRFLKTAAYGHFGRDDPDFTWE 381

HSP 2 Score: 59.6918 bits (143), Expect = 1.786e-51
Identity = 29/29 (100.00%), Postives = 29/29 (100.00%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRSGAYIVRQAAKSIVANG
Sbjct:  269 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 297

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_MUSAC (S-adenosylmethionine synthetase OS=Musa acuminata GN=METK PE=2 SV=1)

HSP 1 Score: 167.548 bits (423), Expect = 5.156e-51
Identity = 81/92 (88.04%), Postives = 87/92 (94.57%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 378
            CIVQVSYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKE+FDFRPG++TINL LKRGG  R+LKTAAYGHFGRDDPDFTWEVVKPL+ EKP A
Sbjct:  303 CIVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKENFDFRPGLITINLGLKRGGY-RYLKTAAYGHFGRDDPDFTWEVVKPLELEKPAA 393

HSP 2 Score: 53.5286 bits (127), Expect = 5.156e-51
Identity = 25/28 (89.29%), Postives = 27/28 (96.43%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVAN 86
            AFSGKDPTKVDRSGAY+ RQAAKSIVA+
Sbjct:  270 AFSGKDPTKVDRSGAYVARQAAKSIVAS 297

The following BLAST results are available for this feature:

BLAST of CK740285 vs. ExPASy Swiss-Prot

BLAST of CK740285 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
METK2_ATRNU	2.142e-52	86.96	S-adenosylmethionine synthetase 2 OS=Atriplex numm...	[more]
METK1_ATRNU	2.142e-52	86.96	S-adenosylmethionine synthetase 1 OS=Atriplex numm...	[more]
METK3_PICSI	2.793e-52	86.52	S-adenosylmethionine synthetase 3 OS=Picea sitchen...	[more]
METK1_DAUCA	3.636e-52	62.86	S-adenosylmethionine synthetase 1 OS=Daucus carota...	[more]
METK1_HORVU	6.185e-52	85.87	S-adenosylmethionine synthetase 1 OS=Hordeum vulga...	[more]
METK1_PEA	8.069e-52	88.64	S-adenosylmethionine synthetase 1 (Fragment) OS=Pi...	[more]
METK1_TRIMO	1.370e-51	85.87	S-adenosylmethionine synthetase 1 OS=Triticum mono...	[more]
METK3_HORVU	1.786e-51	84.78	S-adenosylmethionine synthetase 3 OS=Hordeum vulga...	[more]
METK_CAMSI	1.786e-51	95.00	S-adenosylmethionine synthetase OS=Camellia sinens...	[more]
METK_MUSAC	5.156e-51	88.04	S-adenosylmethionine synthetase OS=Musa acuminata ...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_6428 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE63C12 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK740285 ID=CK740285; Name=CK740285; organism=Citrus sinensis; type=EST; length=563bp
GTGCTTTCTCTGGAAAAGACCCTACTAAGGTGGATAGGAGTGGTGCATAC
ATTGTTAGGCAGGCTGCTAAGAGCATTGTTGCAAACGGGCCTTGCTCGCA
GGTGCATTGTCCAAGTCTCCTATGCCATTGGTGTGCCTGAGCCATTGTCT
GTTTTTGTGGACTCATATGGAACCGGCAAGATTCCTGACAAGGAGATCCT
CAAAATTGTGAAGGAAAGCTTTGATTTCAGGCCAGGAATGATGACCATCA
ATTTGGATCTCAAAAGGGGCGGTAATGGCAGGTTCTTGAAGACTGCTGCC
TATGGACACTTTGGCAGGGATGACCCAGACTTCACTTGGGAAGTTGTGAA
GCCCCTCAAGTGGGAAAAGCCTCAAGCTTAAATTTGAGTTGAAAAGTGAA
AACTATCCCTGCTGTTCAAGTTTGCTTCTATTGGTAGTGGATGAATAATT
TGAATATTATCTTGCTGCTGCTTTGTAATACTAAGCAAATCTCCTGTGTT
CAGAACTCATACATGTTCCCCCATTCAATTCCTTTCTTTTTCTTCTTTCC
CACTTCTTTTTTG