CK740285 | Citrus Genome Database

Overview


Name	CK740285
Unique Name	CK740285
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	563

Libraries


Library Name	Type
Ridge pineapple sweet orange entire seedling	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_OSTTA (S-adenosylmethionine synthetase OS=Ostreococcus tauri GN=METK PE=3 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 3.244e-37
Identity = 59/85 (69.41%), Postives = 69/85 (81.18%), Query Frame = 1

Query:  106 IVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPD-FTWEVVKPL 357
            + Q+SYAIGV +PLS+ VD+YG+GKIPD EIL+ VKE FDFR GM+  +LDLKRGGN R+  TAAYGHFGRDD D FTWE V PL
Sbjct:  302 LFQISYAIGVAQPLSIHVDTYGSGKIPDSEILEKVKEKFDFRAGMIGKSLDLKRGGNKRYQTTAAYGHFGRDDTDVFTWEKVVPL 386

HSP 2 Score: 53.5286 bits (127), Expect = 3.244e-37
Identity = 26/29 (89.66%), Postives = 26/29 (89.66%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRSGAYI RQ AKSIVA G
Sbjct:  268 AFSGKDPTKVDRSGAYIARQVAKSIVAAG 296

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK2_PEA (S-adenosylmethionine synthetase 2 OS=Pisum sativum GN=SAMS2 PE=2 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 9.345e-37
Identity = 54/70 (77.14%), Postives = 62/70 (88.57%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHF 312
            C+VQVSYAIGVPEPLSVFVDSYGTGKIPD+EIL IVKE+FDFRPGM++I+LDL RGGNGRF + +    F
Sbjct:  304 CLVQVSYAIGVPEPLSVFVDSYGTGKIPDREILNIVKEAFDFRPGMISISLDLLRGGNGRFFEDSCIWTF 373

HSP 2 Score: 58.5362 bits (140), Expect = 9.345e-37
Identity = 28/29 (96.55%), Postives = 29/29 (100.00%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRSGAYIVR+AAKSIVANG
Sbjct:  271 AFSGKDPTKVDRSGAYIVREAAKSIVANG 299

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_DICDI (S-adenosylmethionine synthetase OS=Dictyostelium discoideum GN=metK PE=1 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 1.912e-30
Identity = 54/86 (62.79%), Postives = 64/86 (74.42%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLK 360
            C+VQVSY+IGV +PLSVFVD+YGTGK  D EIL I+  +FD RPG +  +L L R     F KTA+ GHFGR+DPDFTWE VK LK
Sbjct:  301 CLVQVSYSIGVAKPLSVFVDTYGTGKKTDAEILAIIDNNFDLRPGCLMRDLQLTR---PIFQKTASGGHFGRNDPDFTWETVKELK 383

HSP 2 Score: 44.669 bits (104), Expect = 1.912e-30
Identity = 22/29 (75.86%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKD TKVDRSGAY  R  AKS+VA G
Sbjct:  268 AFSGKDATKVDRSGAYAARWIAKSLVAAG 296

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_GEOSM (S-adenosylmethionine synthetase OS=Geobacter sp. (strain M21) GN=metK PE=3 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 2.374e-27
Identity = 49/80 (61.25%), Postives = 57/80 (71.25%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C VQV+YAIGV EP+SV VD  GTGKIP K I +IV+E FD RP  +   LDL R     + KTAAYGHFGR+ P+FTWE
Sbjct:  299 CEVQVAYAIGVAEPVSVMVDCNGTGKIPSKRISEIVREVFDMRPRAIIEQLDLLR---PIYRKTAAYGHFGRELPEFTWE 375

HSP 2 Score: 47.3654 bits (111), Expect = 2.374e-27
Identity = 21/33 (63.64%), Postives = 27/33 (81.82%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQ 101
            AFSGKDP+KVDRS AY+ R  AK++VA+G C +
Sbjct:  266 AFSGKDPSKVDRSAAYMGRYVAKNLVASGVCER 298

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_SYNWW (S-adenosylmethionine synthetase OS=Syntrophomonas wolfei subsp. wolfei (strain Goettingen) GN=metK PE=3 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 6.815e-27
Identity = 43/80 (53.75%), Postives = 62/80 (77.50%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QV+YAIGV  PLS+ V+++GTGKI D +I++++K++FD RP  +  +LDL+R     + KTAAYGHFGR+D +FTWE
Sbjct:  306 CEIQVAYAIGVANPLSINVNTFGTGKISDDKIIELIKKNFDLRPAAIIRDLDLRR---PIYRKTAAYGHFGREDAEFTWE 382

HSP 2 Score: 45.8246 bits (107), Expect = 6.815e-27
Identity = 21/29 (72.41%), Postives = 25/29 (86.21%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRS +Y++R  AK+IVA G
Sbjct:  273 AFSGKDPTKVDRSASYMLRYVAKNIVAAG 301

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_CELJU (S-adenosylmethionine synthetase OS=Cellvibrio japonicus (strain Ueda107) GN=metK PE=3 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 6.818e-27
Identity = 46/80 (57.50%), Postives = 61/80 (76.25%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ ++++GTGK+PD EI+K+V+E FD RP  +   L+LKR     + +TAAYGHFGR+ PDFTWE
Sbjct:  296 CEIQVSYAIGVAEPTSISINTFGTGKLPDAEIIKLVREHFDLRPRGLIEMLNLKR---PIYRQTAAYGHFGRELPDFTWE 372

HSP 2 Score: 43.1282 bits (100), Expect = 6.818e-27
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK+IVA G
Sbjct:  263 AFSGKDPSKVDRSAAYAGRYVAKNIVAAG 291

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_GEOUR (S-adenosylmethionine synthetase OS=Geobacter uraniireducens (strain Rf4) GN=metK PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.504e-26
Identity = 48/80 (60.00%), Postives = 57/80 (71.25%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C VQV+YAIGV EP+SV VD+ GTGKIP   I +IV+E FD RP  +   LDL R     + KTAAYGHFGR+ P+FTWE
Sbjct:  299 CEVQVAYAIGVAEPVSVMVDTSGTGKIPSARIAQIVREVFDLRPRAIIEQLDLLR---PIYKKTAAYGHFGRELPEFTWE 375

HSP 2 Score: 45.8246 bits (107), Expect = 1.504e-26
Identity = 21/33 (63.64%), Postives = 26/33 (78.79%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQ 101
            AFSGKDP+KVDRS AY+ R  AK++VA G C +
Sbjct:  266 AFSGKDPSKVDRSAAYMGRYVAKNLVAAGLCEK 298

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_DECAR (S-adenosylmethionine synthetase OS=Dechloromonas aromatica (strain RCB) GN=metK PE=3 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 1.957e-26
Identity = 44/80 (55.00%), Postives = 59/80 (73.75%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C+VQVSYAIGV EP S+ V++YGTGK+ ++ +  +V++ FD RP  +   LDL R     + KTAAYGHFGRD+P+FTWE
Sbjct:  297 CLVQVSYAIGVAEPTSIMVETYGTGKVSNETLTALVRKHFDLRPKGIVNMLDLLR---PIYQKTAAYGHFGRDEPEFTWE 373

HSP 2 Score: 45.0542 bits (105), Expect = 1.957e-26
Identity = 22/33 (66.67%), Postives = 25/33 (75.76%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQ 101
            AFSGKDP+KVDRS AY  R  AK+IVA G  S+
Sbjct:  264 AFSGKDPSKVDRSAAYATRYVAKNIVAAGLASR 296

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_GEOSL (S-adenosylmethionine synthetase OS=Geobacter sulfurreducens GN=metK PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 1.957e-26
Identity = 47/80 (58.75%), Postives = 57/80 (71.25%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C VQV+YAIGV EP+SV VD+ GTGKIP   I +I++E FD RP  +   LDL R     + KTAAYGHFGR+ P+FTWE
Sbjct:  299 CEVQVAYAIGVAEPVSVMVDTAGTGKIPSARIAEIIREVFDLRPRAIIEQLDLLR---PIYRKTAAYGHFGRELPEFTWE 375

HSP 2 Score: 45.8246 bits (107), Expect = 1.957e-26
Identity = 21/33 (63.64%), Postives = 26/33 (78.79%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQ 101
            AFSGKDP+KVDRS AY+ R  AK++VA G C +
Sbjct:  266 AFSGKDPSKVDRSAAYMGRYVAKNLVAAGLCER 298

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_FERNB (S-adenosylmethionine synthetase OS=Fervidobacterium nodosum (strain ATCC 35602 / DSM 5306 / Rt17-B1) GN=metK PE=3 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 2.547e-26
Identity = 45/88 (51.14%), Postives = 66/88 (75.00%), Query Frame = 1

Query:  106 IVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE---VVKPLK 360
            ++QV+YAIGV  P+S+ ++++GT K+ ++++ K+V+E FDFRPG +   LDL R     + KTAAYGHFGR+DPDFTWE   +V+ LK
Sbjct:  307 MIQVAYAIGVARPVSIMINTFGTAKVDEEKLRKVVEEIFDFRPGAIIKKLDLLR---PIYRKTAAYGHFGRNDPDFTWERTDMVRELK 391

HSP 2 Score: 43.5134 bits (101), Expect = 2.547e-26
Identity = 20/29 (68.97%), Postives = 22/29 (75.86%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRS  Y  R  AK++VA G
Sbjct:  273 AFSGKDPTKVDRSAHYFARYVAKNVVAAG 301

The following BLAST results are available for this feature:

BLAST of CK740285 vs. ExPASy Swiss-Prot

BLAST of CK740285 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
METK_OSTTA	3.244e-37	69.41	S-adenosylmethionine synthetase OS=Ostreococcus ta...	[more]
METK2_PEA	9.345e-37	77.14	S-adenosylmethionine synthetase 2 OS=Pisum sativum...	[more]
METK_DICDI	1.912e-30	62.79	S-adenosylmethionine synthetase OS=Dictyostelium d...	[more]
METK_GEOSM	2.374e-27	61.25	S-adenosylmethionine synthetase OS=Geobacter sp. (...	[more]
METK_SYNWW	6.815e-27	53.75	S-adenosylmethionine synthetase OS=Syntrophomonas ...	[more]
METK_CELJU	6.818e-27	57.50	S-adenosylmethionine synthetase OS=Cellvibrio japo...	[more]
METK_GEOUR	1.504e-26	60.00	S-adenosylmethionine synthetase OS=Geobacter urani...	[more]
METK_DECAR	1.957e-26	55.00	S-adenosylmethionine synthetase OS=Dechloromonas a...	[more]
METK_GEOSL	1.957e-26	58.75	S-adenosylmethionine synthetase OS=Geobacter sulfu...	[more]
METK_FERNB	2.547e-26	51.14	S-adenosylmethionine synthetase OS=Fervidobacteriu...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_6428 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE63C12 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK740285 ID=CK740285; Name=CK740285; organism=Citrus sinensis; type=EST; length=563bp
GTGCTTTCTCTGGAAAAGACCCTACTAAGGTGGATAGGAGTGGTGCATAC
ATTGTTAGGCAGGCTGCTAAGAGCATTGTTGCAAACGGGCCTTGCTCGCA
GGTGCATTGTCCAAGTCTCCTATGCCATTGGTGTGCCTGAGCCATTGTCT
GTTTTTGTGGACTCATATGGAACCGGCAAGATTCCTGACAAGGAGATCCT
CAAAATTGTGAAGGAAAGCTTTGATTTCAGGCCAGGAATGATGACCATCA
ATTTGGATCTCAAAAGGGGCGGTAATGGCAGGTTCTTGAAGACTGCTGCC
TATGGACACTTTGGCAGGGATGACCCAGACTTCACTTGGGAAGTTGTGAA
GCCCCTCAAGTGGGAAAAGCCTCAAGCTTAAATTTGAGTTGAAAAGTGAA
AACTATCCCTGCTGTTCAAGTTTGCTTCTATTGGTAGTGGATGAATAATT
TGAATATTATCTTGCTGCTGCTTTGTAATACTAAGCAAATCTCCTGTGTT
CAGAACTCATACATGTTCCCCCATTCAATTCCTTTCTTTTTCTTCTTTCC
CACTTCTTTTTTG