CK740285 | Citrus Genome Database

Overview


Name	CK740285
Unique Name	CK740285
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	563

Libraries


Library Name	Type
Ridge pineapple sweet orange entire seedling	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig1234	contig	Csv1_Contig1234:1050..1610.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_THIDA (S-adenosylmethionine synthetase OS=Thiobacillus denitrificans (strain ATCC 25259) GN=metK PE=3 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 2.548e-26
Identity = 44/81 (54.32%), Postives = 62/81 (76.54%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEV 345
            C+VQVSYAIGV +P S+ VD++GTGKIP+ +I+++++  FD RP  +   LDL R     + +TA+YGHFGRD+P+FTWEV
Sbjct:  298 CMVQVSYAIGVAKPTSLMVDTFGTGKIPEAKIVELIQAHFDLRPKGIVNMLDLLR---PIYTRTASYGHFGRDEPEFTWEV 375

HSP 2 Score: 43.1282 bits (100), Expect = 2.548e-26
Identity = 21/29 (72.41%), Postives = 23/29 (79.31%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDP+KVDRS AY  R  AK+IVA G
Sbjct:  265 AFSGKDPSKVDRSAAYAGRYVAKNIVAAG 293

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_GEOMG (S-adenosylmethionine synthetase OS=Geobacter metallireducens (strain GS-15 / ATCC 53774 / DSM 7210) GN=metK PE=3 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 2.548e-26
Identity = 48/80 (60.00%), Postives = 57/80 (71.25%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C VQV+YAIGV EP+SV VD+ GTGKI  K I +IV+E FD RP  +   LDL R     + KTAAYGHFGR+ P+FTWE
Sbjct:  299 CEVQVAYAIGVAEPVSVMVDTAGTGKISSKRIAEIVREVFDLRPRAIIEQLDLLR---PIYRKTAAYGHFGRELPEFTWE 375

HSP 2 Score: 45.8246 bits (107), Expect = 2.548e-26
Identity = 21/33 (63.64%), Postives = 26/33 (78.79%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQ 101
            AFSGKDP+KVDRS AY+ R  AK++VA G C +
Sbjct:  266 AFSGKDPSKVDRSAAYMGRYVAKNLVAAGLCEK 298

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_NITMU (S-adenosylmethionine synthetase OS=Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849) GN=metK PE=3 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 5.615e-26
Identity = 44/80 (55.00%), Postives = 60/80 (75.00%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C VQ++YAIGV  P+S+ VD++GTGKIPD +I+K+++  FD RP  +   LDL R     + KTAAYGHFGRD+P+F+WE
Sbjct:  297 CEVQMAYAIGVARPVSLMVDTFGTGKIPDDKIVKLIERHFDLRPRGIIHGLDLLR---PIYEKTAAYGHFGRDEPEFSWE 373

HSP 2 Score: 43.1282 bits (100), Expect = 5.615e-26
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQ 101
            AFSGKDP+KVDRS AY  R  AK++VA G  S+
Sbjct:  264 AFSGKDPSKVDRSAAYAGRYVAKNLVAAGIASR 296

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_SYMTH (S-adenosylmethionine synthetase OS=Symbiobacterium thermophilum GN=metK PE=3 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 5.616e-26
Identity = 41/80 (51.25%), Postives = 58/80 (72.50%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C VQV+YAIGV  P+S+ V ++GTGK+PD+ + ++V+E+FD RPG +  +LDL+R     + + AAYGHFGR D D  WE
Sbjct:  308 CEVQVAYAIGVAHPVSILVTTFGTGKLPDERLAQLVRETFDLRPGAIIRDLDLRR---PIYRQVAAYGHFGRPDLDLPWE 384

HSP 2 Score: 47.7506 bits (112), Expect = 5.616e-26
Identity = 23/29 (79.31%), Postives = 24/29 (82.76%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANG 89
            AFSGKDPTKVDRSGAY  R  AK+IVA G
Sbjct:  275 AFSGKDPTKVDRSGAYAARWVAKNIVAAG 303

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_CHRVO (S-adenosylmethionine synthetase OS=Chromobacterium violaceum GN=metK PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 7.312e-26
Identity = 45/80 (56.25%), Postives = 61/80 (76.25%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ VD++GT KIP+++I+++VK+ FD RP  +   LDL R   G   KTAAYGHFGR++P+F+WE
Sbjct:  299 CQIQVSYAIGVAEPTSIAVDTFGTNKIPNEKIVELVKKHFDLRPKGIIQMLDLLRPIYG---KTAAYGHFGREEPEFSWE 375

HSP 2 Score: 43.5134 bits (101), Expect = 7.312e-26
Identity = 22/33 (66.67%), Postives = 24/33 (72.73%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQ 101
            AFSGKDP+KVDRS AY  R  AK+IVA G   Q
Sbjct:  266 AFSGKDPSKVDRSAAYAGRYVAKNIVAAGLARQ 298

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_NITSB (S-adenosylmethionine synthetase OS=Nitratiruptor sp. (strain SB155-2) GN=metK PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 7.313e-26
Identity = 41/78 (52.56%), Postives = 56/78 (71.79%), Query Frame = 1

Query:  109 VQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            VQ++YAIGV EP+S+ VDS+GT K+PD+++   V++ FD  P  +   LDL R     + KTAAYGHFGR+ P+FTWE
Sbjct:  297 VQIAYAIGVVEPVSIMVDSHGTAKVPDEKLEACVRDLFDLTPRGIIKTLDLLR---PIYRKTAAYGHFGRELPEFTWE 371

HSP 2 Score: 51.9878 bits (123), Expect = 7.313e-26
Identity = 23/33 (69.70%), Postives = 27/33 (81.82%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQ 101
            AFSGKDPTKVDRSGAY  R  AK++VA+G C +
Sbjct:  262 AFSGKDPTKVDRSGAYAARYVAKNLVASGACEK 294

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_NEIMF (S-adenosylmethionine synthetase OS=Neisseria meningitidis serogroup C / serotype 2a (strain ATCC 700532 / FAM18) GN=metK PE=3 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 9.518e-26
Identity = 42/80 (52.50%), Postives = 60/80 (75.00%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ +D++GTGKI +++++ +V+E FD RP  +   LDL R     + K+AAYGHFGR++P+FTWE
Sbjct:  299 CQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLVREHFDLRPKGIVQMLDLLR---PIYSKSAAYGHFGREEPEFTWE 375

HSP 2 Score: 45.0542 bits (105), Expect = 9.518e-26
Identity = 22/33 (66.67%), Postives = 25/33 (75.76%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQ 101
            AFSGKDP+KVDRS AY  R  AK+IVA G  +Q
Sbjct:  266 AFSGKDPSKVDRSAAYACRYVAKNIVAAGLATQ 298

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_NEIMB (S-adenosylmethionine synthetase OS=Neisseria meningitidis serogroup B GN=metK PE=3 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 9.518e-26
Identity = 42/80 (52.50%), Postives = 60/80 (75.00%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ +D++GTGKI +++++ +V+E FD RP  +   LDL R     + K+AAYGHFGR++P+FTWE
Sbjct:  299 CQIQVSYAIGVAEPTSISIDTFGTGKISEEKLIALVREHFDLRPKGIVQMLDLLR---PIYSKSAAYGHFGREEPEFTWE 375

HSP 2 Score: 45.0542 bits (105), Expect = 9.518e-26
Identity = 22/33 (66.67%), Postives = 25/33 (75.76%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQ 101
            AFSGKDP+KVDRS AY  R  AK+IVA G  +Q
Sbjct:  266 AFSGKDPSKVDRSAAYACRYVAKNIVAAGLATQ 298

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_NEIMA (S-adenosylmethionine synthetase OS=Neisseria meningitidis serogroup A GN=metK PE=3 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 9.518e-26
Identity = 42/80 (52.50%), Postives = 60/80 (75.00%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ +D++GTGKI +++++ +V+E FD RP  +   LDL R     + K+AAYGHFGR++P+FTWE
Sbjct:  299 CQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLVREHFDLRPKGIVQMLDLLR---PIYSKSAAYGHFGREEPEFTWE 375

HSP 2 Score: 45.0542 bits (105), Expect = 9.518e-26
Identity = 22/33 (66.67%), Postives = 25/33 (75.76%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQ 101
            AFSGKDP+KVDRS AY  R  AK+IVA G  +Q
Sbjct:  266 AFSGKDPSKVDRSAAYACRYVAKNIVAAGLATQ 298

[Close]

BLAST of CK740285 vs. ExPASy Swiss-Prot
Match: METK_NEIM0 (S-adenosylmethionine synthetase OS=Neisseria meningitidis serogroup C (strain 053442) GN=metK PE=3 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 9.518e-26
Identity = 42/80 (52.50%), Postives = 60/80 (75.00%), Query Frame = 1

Query:  103 CIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 342
            C +QVSYAIGV EP S+ +D++GTGKI +++++ +V+E FD RP  +   LDL R     + K+AAYGHFGR++P+FTWE
Sbjct:  299 CQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLVREHFDLRPKGIVQMLDLLR---PIYSKSAAYGHFGREEPEFTWE 375

HSP 2 Score: 45.0542 bits (105), Expect = 9.518e-26
Identity = 22/33 (66.67%), Postives = 25/33 (75.76%), Query Frame = 3

Query:    3 AFSGKDPTKVDRSGAYIVRQAAKSIVANGPCSQ 101
            AFSGKDP+KVDRS AY  R  AK+IVA G  +Q
Sbjct:  266 AFSGKDPSKVDRSAAYACRYVAKNIVAAGLATQ 298

The following BLAST results are available for this feature:

BLAST of CK740285 vs. ExPASy Swiss-Prot

BLAST of CK740285 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
METK_THIDA	2.548e-26	54.32	S-adenosylmethionine synthetase OS=Thiobacillus de...	[more]
METK_GEOMG	2.548e-26	60.00	S-adenosylmethionine synthetase OS=Geobacter metal...	[more]
METK_NITMU	5.615e-26	55.00	S-adenosylmethionine synthetase OS=Nitrosospira mu...	[more]
METK_SYMTH	5.616e-26	51.25	S-adenosylmethionine synthetase OS=Symbiobacterium...	[more]
METK_CHRVO	7.312e-26	56.25	S-adenosylmethionine synthetase OS=Chromobacterium...	[more]
METK_NITSB	7.313e-26	52.56	S-adenosylmethionine synthetase OS=Nitratiruptor s...	[more]
METK_NEIMF	9.518e-26	52.50	S-adenosylmethionine synthetase OS=Neisseria menin...	[more]
METK_NEIMB	9.518e-26	52.50	S-adenosylmethionine synthetase OS=Neisseria menin...	[more]
METK_NEIMA	9.518e-26	52.50	S-adenosylmethionine synthetase OS=Neisseria menin...	[more]
METK_NEIM0	9.518e-26	52.50	S-adenosylmethionine synthetase OS=Neisseria menin...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_6428 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE63C12 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK740285 ID=CK740285; Name=CK740285; organism=Citrus sinensis; type=EST; length=563bp
GTGCTTTCTCTGGAAAAGACCCTACTAAGGTGGATAGGAGTGGTGCATAC
ATTGTTAGGCAGGCTGCTAAGAGCATTGTTGCAAACGGGCCTTGCTCGCA
GGTGCATTGTCCAAGTCTCCTATGCCATTGGTGTGCCTGAGCCATTGTCT
GTTTTTGTGGACTCATATGGAACCGGCAAGATTCCTGACAAGGAGATCCT
CAAAATTGTGAAGGAAAGCTTTGATTTCAGGCCAGGAATGATGACCATCA
ATTTGGATCTCAAAAGGGGCGGTAATGGCAGGTTCTTGAAGACTGCTGCC
TATGGACACTTTGGCAGGGATGACCCAGACTTCACTTGGGAAGTTGTGAA
GCCCCTCAAGTGGGAAAAGCCTCAAGCTTAAATTTGAGTTGAAAAGTGAA
AACTATCCCTGCTGTTCAAGTTTGCTTCTATTGGTAGTGGATGAATAATT
TGAATATTATCTTGCTGCTGCTTTGTAATACTAAGCAAATCTCCTGTGTT
CAGAACTCATACATGTTCCCCCATTCAATTCCTTTCTTTTTCTTCTTTCC
CACTTCTTTTTTG