CK740326 | Citrus Genome Database

Overview


Name	CK740326
Unique Name	CK740326
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	463

Libraries


Library Name	Type
Ridge pineapple sweet orange entire seedling	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig2264	contig	Csv1_Contig2264:281..742.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBE2B_RABIT (Ubiquitin-conjugating enzyme E2 B OS=Oryctolagus cuniculus GN=UBE2B PE=2 SV=1)

HSP 1 Score: 167.548 bits (423), Expect = 9.672e-49
Identity = 74/97 (76.29%), Postives = 90/97 (92.78%), Query Frame = 1

Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVREIVEQSW 375
            FKL ++F+E+YPNKPPTVRF+S+MFHPN+YADGSICLDILQN+WSP YDV++ILTSIQSLL +PNPNSPANS+AA+++ ENKREY +RV  IVEQSW
Sbjct:   53 FKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSW 149

HSP 2 Score: 45.8246 bits (107), Expect = 9.672e-49
Identity = 18/27 (66.67%), Postives = 23/27 (85.19%), Query Frame = 3

Query:    3 GAPQDNSIMLWNAVIFGPDDTPWDGGT 83
            GAP +N+IM WNAVIFGP+ TP++ GT
Sbjct:   26 GAPSENNIMQWNAVIFGPEGTPFEDGT 52

[Close]

BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBE2B_MOUSE (Ubiquitin-conjugating enzyme E2 B OS=Mus musculus GN=Ube2b PE=1 SV=1)

HSP 1 Score: 167.548 bits (423), Expect = 9.672e-49
Identity = 74/97 (76.29%), Postives = 90/97 (92.78%), Query Frame = 1

Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVREIVEQSW 375
            FKL ++F+E+YPNKPPTVRF+S+MFHPN+YADGSICLDILQN+WSP YDV++ILTSIQSLL +PNPNSPANS+AA+++ ENKREY +RV  IVEQSW
Sbjct:   53 FKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSW 149

HSP 2 Score: 45.8246 bits (107), Expect = 9.672e-49
Identity = 18/27 (66.67%), Postives = 23/27 (85.19%), Query Frame = 3

Query:    3 GAPQDNSIMLWNAVIFGPDDTPWDGGT 83
            GAP +N+IM WNAVIFGP+ TP++ GT
Sbjct:   26 GAPSENNIMQWNAVIFGPEGTPFEDGT 52

[Close]

BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBE2B_HUMAN (Ubiquitin-conjugating enzyme E2 B OS=Homo sapiens GN=UBE2B PE=1 SV=1)

HSP 1 Score: 167.548 bits (423), Expect = 9.672e-49
Identity = 74/97 (76.29%), Postives = 90/97 (92.78%), Query Frame = 1

Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVREIVEQSW 375
            FKL ++F+E+YPNKPPTVRF+S+MFHPN+YADGSICLDILQN+WSP YDV++ILTSIQSLL +PNPNSPANS+AA+++ ENKREY +RV  IVEQSW
Sbjct:   53 FKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSW 149

HSP 2 Score: 45.8246 bits (107), Expect = 9.672e-49
Identity = 18/27 (66.67%), Postives = 23/27 (85.19%), Query Frame = 3

Query:    3 GAPQDNSIMLWNAVIFGPDDTPWDGGT 83
            GAP +N+IM WNAVIFGP+ TP++ GT
Sbjct:   26 GAPSENNIMQWNAVIFGPEGTPFEDGT 52

[Close]

BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBE2B_BOVIN (Ubiquitin-conjugating enzyme E2 B OS=Bos taurus GN=UBE2B PE=2 SV=1)

HSP 1 Score: 167.548 bits (423), Expect = 9.672e-49
Identity = 74/97 (76.29%), Postives = 90/97 (92.78%), Query Frame = 1

Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVREIVEQSW 375
            FKL ++F+E+YPNKPPTVRF+S+MFHPN+YADGSICLDILQN+WSP YDV++ILTSIQSLL +PNPNSPANS+AA+++ ENKREY +RV  IVEQSW
Sbjct:   53 FKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSW 149

HSP 2 Score: 45.8246 bits (107), Expect = 9.672e-49
Identity = 18/27 (66.67%), Postives = 23/27 (85.19%), Query Frame = 3

Query:    3 GAPQDNSIMLWNAVIFGPDDTPWDGGT 83
            GAP +N+IM WNAVIFGP+ TP++ GT
Sbjct:   26 GAPSENNIMQWNAVIFGPEGTPFEDGT 52

[Close]

BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBC1_CAEEL (Ubiquitin-conjugating enzyme E2 1 OS=Caenorhabditis elegans GN=ubc-1 PE=1 SV=1)

HSP 1 Score: 171.014 bits (432), Expect = 3.623e-48
Identity = 76/97 (78.35%), Postives = 92/97 (94.85%), Query Frame = 1

Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVREIVEQSW 375
            FKL+L+FTE+YPNKPPTV+F+S+MFHPN+YADGSICLDILQN+WSP YDVAAILTSIQSLL +PNPNSPANS AA+++ EN+REY +RV++IVEQSW
Sbjct:   53 FKLSLEFTEEYPNKPPTVKFISKMFHPNVYADGSICLDILQNRWSPTYDVAAILTSIQSLLDEPNPNSPANSLAAQLYQENRREYEKRVQQIVEQSW 149

HSP 2 Score: 40.4318 bits (93), Expect = 3.623e-48
Identity = 14/27 (51.85%), Postives = 22/27 (81.48%), Query Frame = 3

Query:    3 GAPQDNSIMLWNAVIFGPDDTPWDGGT 83
            GAP +++I+ W A+IFGP +TP++ GT
Sbjct:   26 GAPTEDNILTWEAIIFGPQETPFEDGT 52

[Close]

BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBC2_USTMA (Ubiquitin-conjugating enzyme E2 2 OS=Ustilago maydis GN=UBC2 PE=3 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 6.034e-45
Identity = 72/100 (72.00%), Postives = 84/100 (84.00%), Query Frame = 1

Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVREIVEQSWTAD 384
            FKL L F E YPNKPPTV+F+S+MFHPN+YA+G +CLDILQN+WSP YDVAAILTSIQSLL DPNPNSPAN+EAA ++ EN +EY RRV+  VE SW  D
Sbjct:   53 FKLVLTFDESYPNKPPTVKFLSKMFHPNVYANGELCLDILQNRWSPTYDVAAILTSIQSLLHDPNPNSPANAEAASLYRENMKEYVRRVKATVEASWLDD 152

HSP 2 Score: 43.1282 bits (100), Expect = 6.034e-45
Identity = 17/27 (62.96%), Postives = 23/27 (85.19%), Query Frame = 3

Query:    3 GAPQDNSIMLWNAVIFGPDDTPWDGGT 83
            GAP  +++M+WNAVIFGP DTP++ GT
Sbjct:   26 GAPCADNLMIWNAVIFGPADTPFEDGT 52

[Close]

BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBC2_CRYNE (Ubiquitin-conjugating enzyme E2 2 OS=Cryptococcus neoformans GN=UBC2 PE=3 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 2.272e-44
Identity = 71/97 (73.20%), Postives = 85/97 (87.63%), Query Frame = 1

Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVREIVEQSW 375
            F+LTL FT+ YPNKPPTVRF+S+MFHPNIYA+G +CLDILQN+WSP YDVAAILTS+QSLL DPNP SPAN +AA++F EN +EY RRV++ VE SW
Sbjct:   53 FRLTLTFTDAYPNKPPTVRFISKMFHPNIYANGELCLDILQNRWSPTYDVAAILTSVQSLLNDPNPASPANVDAAQLFKENLKEYERRVKKTVELSW 149

HSP 2 Score: 41.2022 bits (95), Expect = 2.272e-44
Identity = 15/27 (55.56%), Postives = 23/27 (85.19%), Query Frame = 3

Query:    3 GAPQDNSIMLWNAVIFGPDDTPWDGGT 83
            G+P  ++IM+WNAVIFGP +TP++ G+
Sbjct:   26 GSPNPDNIMVWNAVIFGPPETPFEDGS 52

[Close]

BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBC2_SCHPO (Ubiquitin-conjugating enzyme E2 2 OS=Schizosaccharomyces pombe GN=rhp6 PE=1 SV=3)

HSP 1 Score: 151.754 bits (382), Expect = 3.485e-42
Identity = 71/97 (73.20%), Postives = 81/97 (83.51%), Query Frame = 1

Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVREIVEQSW 375
            FKL L F E YPNKPP V+FVS MFHPN+YA+G +CLDILQN+WSP YDVAAILTSIQSLL DPN  SPAN+EAA++  ENK+EY RRVR+ VE SW
Sbjct:   53 FKLVLSFDEQYPNKPPLVKFVSTMFHPNVYANGELCLDILQNRWSPTYDVAAILTSIQSLLNDPNNASPANAEAAQLHRENKKEYVRRVRKTVEDSW 149

HSP 2 Score: 39.6614 bits (91), Expect = 3.485e-42
Identity = 15/26 (57.69%), Postives = 21/26 (80.77%), Query Frame = 3

Query:    6 APQDNSIMLWNAVIFGPDDTPWDGGT 83
            +P  +++MLWNAVI GP DTP++ GT
Sbjct:   27 SPVSDNVMLWNAVIIGPADTPFEDGT 52

[Close]

BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBC2_TRIHA (Ubiquitin-conjugating enzyme E2 2 OS=Trichoderma harzianum GN=UBC2 PE=2 SV=1)

HSP 1 Score: 151.754 bits (382), Expect = 2.223e-41
Identity = 66/97 (68.04%), Postives = 83/97 (85.57%), Query Frame = 1

Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVREIVEQSW 375
            F+L +QF E YPNKPP V+F+S+MFHPN+YA+G +CLDILQN+WSP YDVAA+LTSIQSLL DPN  SPAN EA+ ++ +N+REY +RVRE VE+SW
Sbjct:   53 FRLVMQFEEQYPNKPPQVKFISQMFHPNVYANGELCLDILQNRWSPTYDVAAVLTSIQSLLNDPNTGSPANVEASNLYKDNRREYIKRVRETVERSW 149

HSP 2 Score: 36.965 bits (84), Expect = 2.223e-41
Identity = 14/26 (53.85%), Postives = 20/26 (76.92%), Query Frame = 3

Query:    6 APQDNSIMLWNAVIFGPDDTPWDGGT 83
            +P  +++M WNAVI GP DTP++ GT
Sbjct:   27 SPIPDNVMTWNAVIIGPADTPFEDGT 52

[Close]

BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBC2_NECHA (Ubiquitin-conjugating enzyme E2 2 OS=Nectria haematococca GN=UBC2 PE=3 SV=1)

HSP 1 Score: 149.828 bits (377), Expect = 6.409e-41
Identity = 65/97 (67.01%), Postives = 81/97 (83.51%), Query Frame = 1

Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVREIVEQSW 375
            F+L +QF E YPNKPP V+F+S MFHPN+YA G +CLDILQN+WSP YDVAA+LTSIQSLL DPN  SPAN EA+ ++ +N++EY +RVRE VE+SW
Sbjct:   53 FRLVMQFEEQYPNKPPQVKFISEMFHPNVYATGELCLDILQNRWSPTYDVAAVLTSIQSLLNDPNTGSPANVEASNLYKDNRKEYTKRVRETVEKSW 149

HSP 2 Score: 37.3502 bits (85), Expect = 6.409e-41
Identity = 14/26 (53.85%), Postives = 20/26 (76.92%), Query Frame = 3

Query:    6 APQDNSIMLWNAVIFGPDDTPWDGGT 83
            +P  +++M WNAVI GP DTP++ GT
Sbjct:   27 SPVPDNVMTWNAVIIGPADTPFEDGT 52

The following BLAST results are available for this feature:

BLAST of CK740326 vs. ExPASy Swiss-Prot

BLAST of CK740326 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 225


Match Name	E-value	Identity	Description
UBE2B_RABIT	9.672e-49	76.29	Ubiquitin-conjugating enzyme E2 B OS=Oryctolagus c...	[more]
UBE2B_MOUSE	9.672e-49	76.29	Ubiquitin-conjugating enzyme E2 B OS=Mus musculus ...	[more]
UBE2B_HUMAN	9.672e-49	76.29	Ubiquitin-conjugating enzyme E2 B OS=Homo sapiens ...	[more]
UBE2B_BOVIN	9.672e-49	76.29	Ubiquitin-conjugating enzyme E2 B OS=Bos taurus GN...	[more]
UBC1_CAEEL	3.623e-48	78.35	Ubiquitin-conjugating enzyme E2 1 OS=Caenorhabditi...	[more]
UBC2_USTMA	6.034e-45	72.00	Ubiquitin-conjugating enzyme E2 2 OS=Ustilago mayd...	[more]
UBC2_CRYNE	2.272e-44	73.20	Ubiquitin-conjugating enzyme E2 2 OS=Cryptococcus ...	[more]
UBC2_SCHPO	3.485e-42	73.20	Ubiquitin-conjugating enzyme E2 2 OS=Schizosacchar...	[more]
UBC2_TRIHA	2.223e-41	68.04	Ubiquitin-conjugating enzyme E2 2 OS=Trichoderma h...	[more]
UBC2_NECHA	6.409e-41	67.01	Ubiquitin-conjugating enzyme E2 2 OS=Nectria haema...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_6470 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE63H07 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK740326 ID=CK740326; Name=CK740326; organism=Citrus sinensis; type=EST; length=463bp
GTGGTGCTCCTCAGGACAACAGTATAATGCTTTGGAATGCTGTTATATTC
GGGCCGGATGACACCCCATGGGATGGAGGTACCGTTTAAATTGACACTTC
AGTTCACAGAGGATTATCCAAATAAGCCACCAACAGTGCGCTTTGTTTCT
CGGATGTTCCATCCAAATATTTATGCGGATGGAAGCATTTGCTTGGACAT
TCTACAAAATCAGTGGAGTCCAATTTACGATGTAGCAGCAATACTCACGT
CAATTCAGTCATTGCTTTGTGACCCAAATCCAAACTCTCCTGCAAATTCG
GAGGCAGCACGGATGTTTAGCGAGAACAAGCGTGAATACCATAGGAGAGT
ACGGGAAATTGTTGAGCAGAGTTGGACTGCTGACTAGTGGCAACTTGTTG
GAATGTAGGGAACTTCAAAAATAGTCCAATGGCTTGTTCTAAATAACATT
TAAGATTCAAAAG