CK740326 | Citrus Genome Database

Overview


Name	CK740326
Unique Name	CK740326
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	463

Libraries


Library Name	Type
Ridge pineapple sweet orange entire seedling	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig2264	contig	Csv1_Contig2264:281..742.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBE2N_RAT (Ubiquitin-conjugating enzyme E2 N OS=Rattus norvegicus GN=Ube2n PE=1 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 1.862e-18
Identity = 36/84 (42.86%), Postives = 55/84 (65.48%), Query Frame = 1

Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKRE 336
            FKL L   E+YP   P VRF+++++HPN+   G ICLDIL+++WSP   +  +L SIQ+LL  PNP+ P  ++ A  +  N+ +
Sbjct:   52 FKLELFLPEEYPMAAPKVRFMTKIYHPNVDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKSNEAQ 135

HSP 2 Score: 28.8758 bits (63), Expect = 1.862e-18
Identity = 10/25 (40.00%), Postives = 18/25 (72.00%), Query Frame = 3

Query:    9 PQDNSIMLWNAVIFGPDDTPWDGGT 83
            P +++   ++ VI GP D+P++GGT
Sbjct:   27 PDESNARYFHVVIAGPQDSPFEGGT 51

[Close]

BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBE2N_PONAB (Ubiquitin-conjugating enzyme E2 N OS=Pongo abelii GN=UBE2N PE=2 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 2.422e-18
Identity = 36/84 (42.86%), Postives = 55/84 (65.48%), Query Frame = 1

Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKRE 336
            FKL L   E+YP   P VRF+++++HPN+   G ICLDIL+++WSP   +  +L SIQ+LL  PNP+ P  ++ A  +  N+ +
Sbjct:   52 FKLELFLPEEYPMAAPKVRFMTKIYHPNVDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQ 135

HSP 2 Score: 28.8758 bits (63), Expect = 2.422e-18
Identity = 10/25 (40.00%), Postives = 18/25 (72.00%), Query Frame = 3

Query:    9 PQDNSIMLWNAVIFGPDDTPWDGGT 83
            P +++   ++ VI GP D+P++GGT
Sbjct:   27 PDESNARYFHVVIAGPQDSPFEGGT 51

[Close]

BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBE2N_MOUSE (Ubiquitin-conjugating enzyme E2 N OS=Mus musculus GN=Ube2n PE=1 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 2.422e-18
Identity = 36/84 (42.86%), Postives = 55/84 (65.48%), Query Frame = 1

Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKRE 336
            FKL L   E+YP   P VRF+++++HPN+   G ICLDIL+++WSP   +  +L SIQ+LL  PNP+ P  ++ A  +  N+ +
Sbjct:   52 FKLELFLPEEYPMAAPKVRFMTKIYHPNVDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQ 135

HSP 2 Score: 28.8758 bits (63), Expect = 2.422e-18
Identity = 10/25 (40.00%), Postives = 18/25 (72.00%), Query Frame = 3

Query:    9 PQDNSIMLWNAVIFGPDDTPWDGGT 83
            P +++   ++ VI GP D+P++GGT
Sbjct:   27 PDESNARYFHVVIAGPQDSPFEGGT 51

[Close]

BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBE2N_HUMAN (Ubiquitin-conjugating enzyme E2 N OS=Homo sapiens GN=UBE2N PE=1 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 2.422e-18
Identity = 36/84 (42.86%), Postives = 55/84 (65.48%), Query Frame = 1

Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKRE 336
            FKL L   E+YP   P VRF+++++HPN+   G ICLDIL+++WSP   +  +L SIQ+LL  PNP+ P  ++ A  +  N+ +
Sbjct:   52 FKLELFLPEEYPMAAPKVRFMTKIYHPNVDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQ 135

HSP 2 Score: 28.8758 bits (63), Expect = 2.422e-18
Identity = 10/25 (40.00%), Postives = 18/25 (72.00%), Query Frame = 3

Query:    9 PQDNSIMLWNAVIFGPDDTPWDGGT 83
            P +++   ++ VI GP D+P++GGT
Sbjct:   27 PDESNARYFHVVIAGPQDSPFEGGT 51

[Close]

BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBE2N_BOVIN (Ubiquitin-conjugating enzyme E2 N OS=Bos taurus GN=UBE2N PE=2 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 2.422e-18
Identity = 36/84 (42.86%), Postives = 55/84 (65.48%), Query Frame = 1

Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKRE 336
            FKL L   E+YP   P VRF+++++HPN+   G ICLDIL+++WSP   +  +L SIQ+LL  PNP+ P  ++ A  +  N+ +
Sbjct:   52 FKLELFLPEEYPMAAPKVRFMTKIYHPNVDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQ 135

HSP 2 Score: 28.8758 bits (63), Expect = 2.422e-18
Identity = 10/25 (40.00%), Postives = 18/25 (72.00%), Query Frame = 3

Query:    9 PQDNSIMLWNAVIFGPDDTPWDGGT 83
            P +++   ++ VI GP D+P++GGT
Sbjct:   27 PDESNARYFHVVIAGPQDSPFEGGT 51

[Close]

BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBC_ASFB7 (Ubiquitin-conjugating enzyme E2 OS=African swine fever virus (strain Badajoz 1971 Vero-adapted) GN=UBC PE=2 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 3.110e-18
Identity = 43/109 (39.45%), Postives = 62/109 (56.88%), Query Frame = 1

Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQ--------WSPIYDVAAILTSIQSLLCDPNPNSPANSEAAR-----MFSENKREYHRRVREIVEQS 372
            FK  + F  +YP  PP + F S M+HPNIY DG +C+ IL           WSP   +  IL S+ SLL +PNP+SPAN +AA+     ++ E+   Y   V++ V++S
Sbjct:   50 FKAKVAFPPEYPYAPPKLTFTSEMWHPNIYPDGRLCISILHGDNAEEQGMTWSPAQKIDTILLSVISLLNEPNPDSPANVDAAKSYRKYVYKEDLESYPMEVKKTVKKS 158

HSP 2 Score: 27.335 bits (59), Expect = 3.110e-18
Identity = 9/22 (40.91%), Postives = 16/22 (72.73%), Query Frame = 3

Query:   15 DNSIMLWNAVIFGPDDTPWDGG 80
            +N+I  W+ ++ GP DT ++GG
Sbjct:   27 ENNITEWDVILRGPPDTLYEGG 48

[Close]

BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: Y1A1_ENCCU (Probable ubiquitin-conjugating enzyme E2 ECU01_1010 OS=Encephalitozoon cuniculi GN=ECU01_1010 PE=1 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 3.741e-18
Identity = 46/126 (36.51%), Postives = 72/126 (57.14%), Query Frame = 1

Query:   34 GMLLYSGRMTPHGMEVPFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ--------------NQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVREIVEQ 369
            G  LY+GR+         K  ++F   YP +PPT++FVS+MFHPNIY DG +C+ IL+              ++W+P+ ++  I+ SI  +L  PN +SPAN +A+ M+ +N  EY + V  I  +
Sbjct:   76 GDSLYAGRI--------LKAVMKFPSSYPLRPPTLKFVSKMFHPNIYEDGKMCISILEEDKQQDSSVFGDPKDKWTPVQNIRTIVMSIVVILNSPNISSPANVDASVMYRDNPEEYIKEVIRIARE 193

[Close]

BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBC7_YEAST (Ubiquitin-conjugating enzyme E2-18 kDa OS=Saccharomyces cerevisiae GN=UBC7 PE=1 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 4.082e-18
Identity = 40/111 (36.04%), Postives = 62/111 (55.86%), Query Frame = 1

Query:   61 TPHGMEVPFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDIL-------------QNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVR 354
            TP+   V F   L+F +DYP  PP + F   + HPNIY +G +C+ IL             + +WSP+  V  IL S+ S+L +PN  S AN +A  ++ +N+ E+ R+V+
Sbjct:   47 TPYADGV-FNAKLEFPKDYPLSPPKLTFTPSILHPNIYPNGEVCISILHSPGDDPNMYELAEERWSPVQSVEKILLSVMSMLSEPNIESGANIDACILWRDNRPEFERQVK 156

HSP 2 Score: 30.8018 bits (68), Expect = 4.082e-18
Identity = 11/26 (42.31%), Postives = 17/26 (65.38%), Query Frame = 3

Query:    3 GAPQDNSIMLWNAVIFGPDDTPWDGG 80
            G   +N+I +W+ +I GP DTP+  G
Sbjct:   27 GPKSENNIFIWDCLIQGPPDTPYADG 52

[Close]

BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBC9_MESAU (SUMO-conjugating enzyme UBC9 OS=Mesocricetus auratus GN=UBE2I PE=2 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 4.088e-18
Identity = 37/92 (40.22%), Postives = 55/92 (59.78%), Query Frame = 1

Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ--NQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVR 354
            FKL + F +DYP+ PP  +F   +FHPN+Y  G++CL IL+    W P   +  +   IQ LL +PN   PA +EA  ++ +N+ EY +R R
Sbjct:   58 FKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITINQLFIGIQELLNEPNIQEPAQAEAYTIYCQNRVEYEKRFR 149

HSP 2 Score: 26.1794 bits (56), Expect = 4.088e-18
Identity = 9/20 (45.00%), Postives = 12/20 (60.00%), Query Frame = 3

Query:   21 SIMLWNAVIFGPDDTPWDGG 80
            ++M W   I G   TPW+GG
Sbjct:   37 NLMNWECAIPGKKGTPWEGG 56

[Close]

BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBC9_PAGMA (SUMO-conjugating enzyme UBC9 OS=Pagrus major GN=ube2i PE=2 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 5.316e-18
Identity = 40/92 (43.48%), Postives = 57/92 (61.96%), Query Frame = 1

Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ--NQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVR 354
            FKL + F +DYP+ PP  +F   +FHPN+Y  G++CL IL+    W P   +  IL  IQ LL +PN   PA +EA  +F +++ EY +RVR
Sbjct:   58 FKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIFCQDRTEYEKRVR 149

HSP 2 Score: 21.1718 bits (43), Expect = 5.316e-18
Identity = 8/20 (40.00%), Postives = 11/20 (55.00%), Query Frame = 3

Query:   21 SIMLWNAVIFGPDDTPWDGG 80
            ++M     I G   TPW+GG
Sbjct:   37 NLMNGECAIPGKKGTPWEGG 56

The following BLAST results are available for this feature:

BLAST of CK740326 vs. ExPASy Swiss-Prot

BLAST of CK740326 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 225


Match Name	E-value	Identity	Description
UBE2N_RAT	1.862e-18	42.86	Ubiquitin-conjugating enzyme E2 N OS=Rattus norveg...	[more]
UBE2N_PONAB	2.422e-18	42.86	Ubiquitin-conjugating enzyme E2 N OS=Pongo abelii ...	[more]
UBE2N_MOUSE	2.422e-18	42.86	Ubiquitin-conjugating enzyme E2 N OS=Mus musculus ...	[more]
UBE2N_HUMAN	2.422e-18	42.86	Ubiquitin-conjugating enzyme E2 N OS=Homo sapiens ...	[more]
UBE2N_BOVIN	2.422e-18	42.86	Ubiquitin-conjugating enzyme E2 N OS=Bos taurus GN...	[more]
UBC_ASFB7	3.110e-18	39.45	Ubiquitin-conjugating enzyme E2 OS=African swine f...	[more]
Y1A1_ENCCU	3.741e-18	36.51	Probable ubiquitin-conjugating enzyme E2 ECU01_101...	[more]
UBC7_YEAST	4.082e-18	36.04	Ubiquitin-conjugating enzyme E2-18 kDa OS=Saccharo...	[more]
UBC9_MESAU	4.088e-18	40.22	SUMO-conjugating enzyme UBC9 OS=Mesocricetus aurat...	[more]
UBC9_PAGMA	5.316e-18	43.48	SUMO-conjugating enzyme UBC9 OS=Pagrus major GN=ub...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_6470 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE63H07 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK740326 ID=CK740326; Name=CK740326; organism=Citrus sinensis; type=EST; length=463bp
GTGGTGCTCCTCAGGACAACAGTATAATGCTTTGGAATGCTGTTATATTC
GGGCCGGATGACACCCCATGGGATGGAGGTACCGTTTAAATTGACACTTC
AGTTCACAGAGGATTATCCAAATAAGCCACCAACAGTGCGCTTTGTTTCT
CGGATGTTCCATCCAAATATTTATGCGGATGGAAGCATTTGCTTGGACAT
TCTACAAAATCAGTGGAGTCCAATTTACGATGTAGCAGCAATACTCACGT
CAATTCAGTCATTGCTTTGTGACCCAAATCCAAACTCTCCTGCAAATTCG
GAGGCAGCACGGATGTTTAGCGAGAACAAGCGTGAATACCATAGGAGAGT
ACGGGAAATTGTTGAGCAGAGTTGGACTGCTGACTAGTGGCAACTTGTTG
GAATGTAGGGAACTTCAAAAATAGTCCAATGGCTTGTTCTAAATAACATT
TAAGATTCAAAAG