CK740326

Overview
NameCK740326
Unique NameCK740326
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length463
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBC13_YEAST (Ubiquitin-conjugating enzyme E2 13 OS=Saccharomyces cerevisiae GN=UBC13 PE=1 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 6.925e-18
Identity = 38/91 (41.76%), Postives = 58/91 (63.74%), Query Frame = 1 
Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVRE 357
            F+L L   +DYP + P VRF+++++HPNI   G ICLD+L+  WSP   +  +L SIQ+LL  PNPN P  ++ A  + +N++    + RE
Sbjct:   52 FELELYLPDDYPMEAPKVRFLTKIYHPNIDRLGRICLDVLKTNWSPALQIRTVLLSIQALLASPNPNDPLANDVAEDWIKNEQGAKAKARE 142

HSP 2 Score: 23.483 bits (49), Expect = 6.925e-18
Identity = 7/24 (29.17%), Postives = 15/24 (62.50%), Query Frame = 3 
Query:    9 PQDNSIMLWNAVIFGPDDTPWDGG 80
            P D+++  +   I GP+ +P++ G
Sbjct:   27 PHDDNLRYFQVTIEGPEQSPYEDG 50
[Close]
BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBC_ASFWA (Ubiquitin-conjugating enzyme E2 OS=African swine fever virus (isolate Warthog/Namibia/Wart80/1980) GN=UBC PE=2 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 8.883e-18
Identity = 44/109 (40.37%), Postives = 60/109 (55.05%), Query Frame = 1 
Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQ--------WSPIYDVAAILTSIQSLLCDPNPNSPANSEAAR-----MFSENKREYHRRVREIVEQS 372
            FK  + F  +YP  PP + F S M+HPNIY DG +C+ IL           WSP   +  IL S+ SLL +PNP+SPAN +AA+     M  E+   Y   V+  V++S
Sbjct:   50 FKAKVAFPPEYPYAPPRLTFTSEMWHPNIYPDGKLCISILHGDNAEEQGMTWSPAQKIDTILLSVISLLNEPNPDSPANVDAAKSYRKLMHKEDLESYPMEVKRTVKKS 158

HSP 2 Score: 25.7942 bits (55), Expect = 8.883e-18
Identity = 8/22 (36.36%), Postives = 15/22 (68.18%), Query Frame = 3 
Query:   15 DNSIMLWNAVIFGPDDTPWDGG 80
            +N +  W+ ++ GP DT ++GG
Sbjct:   27 ENDMTEWDVILRGPPDTLYEGG 48
[Close]
BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBC_ASFM2 (Ubiquitin-conjugating enzyme E2 OS=African swine fever virus (isolate Tick/Malawi/Lil 20-1/1983) GN=UBC PE=2 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 1.156e-17
Identity = 42/109 (38.53%), Postives = 63/109 (57.80%), Query Frame = 1 
Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQ--------WSPIYDVAAILTSIQSLLCDPNPNSPANSEAAR-----MFSENKREYHRRVREIVEQS 372
            FK  + F   YP +PP + F S M+HPNIY+DG +C+ IL           WSP   +  +L S+ SLL +PNP+SPAN +AA+     ++ E+   Y   V++ V++S
Sbjct:   50 FKAKIVFPPKYPYEPPRLTFTSEMWHPNIYSDGKLCISILHGDNAEEQGMTWSPAQKIDTVLLSVISLLNEPNPDSPANVDAAKSYRKYLYKEDLESYPMEVKKTVKKS 158

HSP 2 Score: 24.6386 bits (52), Expect = 1.156e-17
Identity = 7/22 (31.82%), Postives = 16/22 (72.73%), Query Frame = 3 
Query:   15 DNSIMLWNAVIFGPDDTPWDGG 80
            ++++  W+ ++ GP DT ++GG
Sbjct:   27 EDNLTEWDVILKGPPDTLYEGG 48
[Close]
BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBC_ASFP4 (Ubiquitin-conjugating enzyme E2 OS=African swine fever virus (isolate Tick/South Africa/Pretoriuskop Pr4/1996) GN=UBC PE=2 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.953e-17
Identity = 42/109 (38.53%), Postives = 62/109 (56.88%), Query Frame = 1 
Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQ--------WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMF-----SENKREYHRRVREIVEQS 372
            FK  + F  +YP +PP + F S M+HPNIY+DG +C+ IL           WSP+  +  IL S+ SLL +PN +SPAN +AA+ +      E+   Y   V+  V++S
Sbjct:   50 FKAKIGFPPNYPYEPPRLTFTSEMWHPNIYSDGRVCISILHGDNAEEQGMTWSPVQKIDTILLSVISLLNEPNTDSPANVDAAKSYRRFVHKEDLESYPMEVKRTVKKS 158

HSP 2 Score: 25.7942 bits (55), Expect = 1.953e-17
Identity = 8/22 (36.36%), Postives = 16/22 (72.73%), Query Frame = 3 
Query:   15 DNSIMLWNAVIFGPDDTPWDGG 80
            +N++  W+ ++ GP DT ++GG
Sbjct:   27 ENNMTEWDVILKGPADTLYEGG 48
[Close]
BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UB2E2_MOUSE (Ubiquitin-conjugating enzyme E2 E2 OS=Mus musculus GN=Ube2e2 PE=2 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 1.959e-17
Identity = 40/91 (43.96%), Postives = 55/91 (60.44%), Query Frame = 1 
Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVRE 357
            F L + F+ DYP KPP V F +R++H NI + G ICLDIL++ WSP   ++ +L SI SLL D NP  P     A  +  N+ E+ R  R+
Sbjct:  104 FFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQ 194

HSP 2 Score: 25.409 bits (54), Expect = 1.959e-17
Identity = 9/29 (31.03%), Postives = 17/29 (58.62%), Query Frame = 3 
Query:    9 PQDNSIMLWNAVIFGPDDTPWDGGTV*ID 95
            P+ ++I  W + I GP  + ++GG   +D
Sbjct:   79 PKGDNIYEWRSTILGPPGSVYEGGVFFLD 107
[Close]
BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UB2E2_HUMAN (Ubiquitin-conjugating enzyme E2 E2 OS=Homo sapiens GN=UBE2E2 PE=1 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 1.959e-17
Identity = 40/91 (43.96%), Postives = 55/91 (60.44%), Query Frame = 1 
Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVRE 357
            F L + F+ DYP KPP V F +R++H NI + G ICLDIL++ WSP   ++ +L SI SLL D NP  P     A  +  N+ E+ R  R+
Sbjct:  104 FFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQ 194

HSP 2 Score: 25.409 bits (54), Expect = 1.959e-17
Identity = 9/29 (31.03%), Postives = 17/29 (58.62%), Query Frame = 3 
Query:    9 PQDNSIMLWNAVIFGPDDTPWDGGTV*ID 95
            P+ ++I  W + I GP  + ++GG   +D
Sbjct:   79 PKGDNIYEWRSTILGPPGSVYEGGVFFLD 107
[Close]
BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UB2E1_MOUSE (Ubiquitin-conjugating enzyme E2 E1 OS=Mus musculus GN=Ube2e1 PE=2 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 1.961e-17
Identity = 40/91 (43.96%), Postives = 55/91 (60.44%), Query Frame = 1 
Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVRE 357
            F L + FT +YP KPP V F +R++H NI + G ICLDIL++ WSP   ++ +L SI SLL D NP  P     A  +  N+ E+ R  R+
Sbjct:   96 FFLDITFTPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQ 186

HSP 2 Score: 25.409 bits (54), Expect = 1.961e-17
Identity = 9/29 (31.03%), Postives = 17/29 (58.62%), Query Frame = 3 
Query:    9 PQDNSIMLWNAVIFGPDDTPWDGGTV*ID 95
            P+ ++I  W + I GP  + ++GG   +D
Sbjct:   71 PKGDNIYEWRSTILGPPGSVYEGGVFFLD 99
[Close]
BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UB2E1_HUMAN (Ubiquitin-conjugating enzyme E2 E1 OS=Homo sapiens GN=UBE2E1 PE=1 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 1.961e-17
Identity = 40/91 (43.96%), Postives = 55/91 (60.44%), Query Frame = 1 
Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVRE 357
            F L + FT +YP KPP V F +R++H NI + G ICLDIL++ WSP   ++ +L SI SLL D NP  P     A  +  N+ E+ R  R+
Sbjct:   96 FFLDITFTPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQ 186

HSP 2 Score: 25.409 bits (54), Expect = 1.961e-17
Identity = 9/29 (31.03%), Postives = 17/29 (58.62%), Query Frame = 3 
Query:    9 PQDNSIMLWNAVIFGPDDTPWDGGTV*ID 95
            P+ ++I  W + I GP  + ++GG   +D
Sbjct:   71 PKGDNIYEWRSTILGPPGSVYEGGVFFLD 99
[Close]
BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBC21_ARATH (Probable ubiquitin carrier protein E2 21 OS=Arabidopsis thaliana GN=UBC21 PE=1 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.976e-17
Identity = 32/78 (41.03%), Postives = 52/78 (66.67%), Query Frame = 1 
Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNI-YADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARM 315
            F+L     E YP +PP VRF++++FHPN+ +  G ICLDIL+N WSP + + ++  +I +L+  P P+SP N ++  +
Sbjct:   54 FQLAFSVPEPYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNL 131

HSP 2 Score: 31.5722 bits (70), Expect = 1.976e-17
Identity = 13/30 (43.33%), Postives = 19/30 (63.33%), Query Frame = 3 
Query:   15 DNSIMLWNAVIFGPDDTPWDGGTV*IDTSV 104
            D +I  W A+I GP +TP++GG   +  SV
Sbjct:   31 DTNIFKWTALIKGPSETPYEGGVFQLAFSV 60
[Close]
BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBCB_DICDI (Ubiquitin conjugating enzyme E2 B OS=Dictyostelium discoideum GN=ubcB PE=1 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 1.982e-17
Identity = 34/92 (36.96%), Postives = 62/92 (67.39%), Query Frame = 1 
Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNI-YADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVRE 357
            F + +    DYP KPPT++F ++++HPNI  +DG+IC ++  + WSP   +  +LT+I+S+L DPNP++P  +E A+ F  ++  +++  +E
Sbjct:   51 FSMDIDIPADYPFKPPTLKFTTKIYHPNIKTSDGAICAEVF-STWSPQLKILDVLTTIRSILTDPNPDNPLETEIAQQFKTDRNAFNKTAKE 141

HSP 2 Score: 26.1794 bits (56), Expect = 1.982e-17
Identity = 8/30 (26.67%), Postives = 18/30 (60.00%), Query Frame = 3 
Query:   15 DNSIMLWNAVIFGPDDTPWDGGTV*IDTSV 104
            D+++  W A + GP+ +P++ G   +D  +
Sbjct:   28 DDNLQKWKATVQGPEGSPFEKGVFSMDIDI 57
The following BLAST results are available for this feature:
BLAST of CK740326 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 225
Match NameE-valueIdentityDescription
UBC13_YEAST6.925e-1841.76Ubiquitin-conjugating enzyme E2 13 OS=Saccharomyce... [more]
UBC_ASFWA8.883e-1840.37Ubiquitin-conjugating enzyme E2 OS=African swine f... [more]
UBC_ASFM21.156e-1738.53Ubiquitin-conjugating enzyme E2 OS=African swine f... [more]
UBC_ASFP41.953e-1738.53Ubiquitin-conjugating enzyme E2 OS=African swine f... [more]
UB2E2_MOUSE1.959e-1743.96Ubiquitin-conjugating enzyme E2 E2 OS=Mus musculus... [more]
UB2E2_HUMAN1.959e-1743.96Ubiquitin-conjugating enzyme E2 E2 OS=Homo sapiens... [more]
UB2E1_MOUSE1.961e-1743.96Ubiquitin-conjugating enzyme E2 E1 OS=Mus musculus... [more]
UB2E1_HUMAN1.961e-1743.96Ubiquitin-conjugating enzyme E2 E1 OS=Homo sapiens... [more]
UBC21_ARATH1.976e-1741.03Probable ubiquitin carrier protein E2 21 OS=Arabid... [more]
UBCB_DICDI1.982e-1736.96Ubiquitin conjugating enzyme E2 B OS=Dictyostelium... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_6470 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE63H07 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK740326 ID=CK740326; Name=CK740326; organism=Citrus sinensis; type=EST; length=463bp
GTGGTGCTCCTCAGGACAACAGTATAATGCTTTGGAATGCTGTTATATTC
GGGCCGGATGACACCCCATGGGATGGAGGTACCGTTTAAATTGACACTTC
AGTTCACAGAGGATTATCCAAATAAGCCACCAACAGTGCGCTTTGTTTCT
CGGATGTTCCATCCAAATATTTATGCGGATGGAAGCATTTGCTTGGACAT
TCTACAAAATCAGTGGAGTCCAATTTACGATGTAGCAGCAATACTCACGT
CAATTCAGTCATTGCTTTGTGACCCAAATCCAAACTCTCCTGCAAATTCG
GAGGCAGCACGGATGTTTAGCGAGAACAAGCGTGAATACCATAGGAGAGT
ACGGGAAATTGTTGAGCAGAGTTGGACTGCTGACTAGTGGCAACTTGTTG
GAATGTAGGGAACTTCAAAAATAGTCCAATGGCTTGTTCTAAATAACATT
TAAGATTCAAAAG
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