CK740326 | Citrus Genome Database

Overview


Name	CK740326
Unique Name	CK740326
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	463

Libraries


Library Name	Type
Ridge pineapple sweet orange entire seedling	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig2264	contig	Csv1_Contig2264:281..742.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBC31_ARATH (Probable ubiquitin carrier protein E2 31 OS=Arabidopsis thaliana GN=UBC31 PE=2 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 3.608e-20
Identity = 38/91 (41.76%), Postives = 59/91 (64.84%), Query Frame = 1

Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVRE 357
            F L+++F  DYP KPP   F + ++HPNI  +GSIC++IL+++W+P   V  +L SI  LL  PNP+ P   E  ++F  N+ ++ +R RE
Sbjct:   56 FNLSIKFPTDYPFKPPKFTFKTPIYHPNINDEGSICMNILKDKWTPALMVEKVLLSILLLLEKPNPDDPLVPEIGQLFKNNRFQFDQRARE 146

HSP 2 Score: 32.7278 bits (73), Expect = 3.608e-20
Identity = 13/21 (61.90%), Postives = 16/21 (76.19%), Query Frame = 3

Query:   18 NSIMLWNAVIFGPDDTPWDGG 80
            N+I  W AVI GPD TP++GG
Sbjct:   34 NNIYEWTAVIRGPDGTPYEGG 54

BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBC9_DICDI (Sumo-conjugating enzyme ubc9 OS=Dictyostelium discoideum GN=ubc9 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 7.935e-20
Identity = 41/92 (44.57%), Postives = 56/92 (60.87%), Query Frame = 1

Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQ--WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVR 354
            + L ++FTEDYP+KPP  RF    FHPN+Y  G++CL IL  +  W P   +  +L  IQ LL +PNP SPA      +F  NK EY ++V+
Sbjct:   58 YPLIMEFTEDYPSKPPKCRFPKDFFHPNVYPSGTVCLSILNEEADWKPSVTIKTVLLGIQDLLDNPNPKSPAQQLPIHLFLTNKEEYDKKVK 149

HSP 2 Score: 23.0978 bits (48), Expect = 7.935e-20
Identity = 8/20 (40.00%), Postives = 12/20 (60.00%), Query Frame = 3

Query:   21 SIMLWNAVIFGPDDTPWDGG 80
            ++ +WN  I G   T W+GG
Sbjct:   37 NLYVWNCGIPGKTKTNWEGG 56

BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBC9_XENTR (SUMO-conjugating enzyme UBC9 OS=Xenopus tropicalis GN=ube2i PE=2 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.746e-19
Identity = 40/92 (43.48%), Postives = 57/92 (61.96%), Query Frame = 1

Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ--NQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVR 354
            FKL + F +DYP+ PP  +F   +FHPN+Y  G++CL IL+    W P   +  IL  IQ LL +PN   PA +EA  ++ +N+ EY +RVR
Sbjct:   58 FKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVR 149

HSP 2 Score: 26.1794 bits (56), Expect = 1.746e-19
Identity = 9/20 (45.00%), Postives = 12/20 (60.00%), Query Frame = 3

Query:   21 SIMLWNAVIFGPDDTPWDGG 80
            ++M W   I G   TPW+GG
Sbjct:   37 NLMNWECAIPGKKGTPWEGG 56

BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBC9_XENLA (SUMO-conjugating enzyme UBC9 OS=Xenopus laevis GN=ube2i PE=1 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.746e-19
Identity = 40/92 (43.48%), Postives = 57/92 (61.96%), Query Frame = 1

Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ--NQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVR 354
            FKL + F +DYP+ PP  +F   +FHPN+Y  G++CL IL+    W P   +  IL  IQ LL +PN   PA +EA  ++ +N+ EY +RVR
Sbjct:   58 FKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVR 149

HSP 2 Score: 26.1794 bits (56), Expect = 1.746e-19
Identity = 9/20 (45.00%), Postives = 12/20 (60.00%), Query Frame = 3

Query:   21 SIMLWNAVIFGPDDTPWDGG 80
            ++M W   I G   TPW+GG
Sbjct:   37 NLMNWECAIPGKKGTPWEGG 56

BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBC9_SPETR (SUMO-conjugating enzyme UBC9 OS=Spermophilus tridecemlineatus GN=UBE2I PE=1 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.746e-19
Identity = 40/92 (43.48%), Postives = 57/92 (61.96%), Query Frame = 1

Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ--NQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVR 354
            FKL + F +DYP+ PP  +F   +FHPN+Y  G++CL IL+    W P   +  IL  IQ LL +PN   PA +EA  ++ +N+ EY +RVR
Sbjct:   58 FKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVR 149

HSP 2 Score: 26.1794 bits (56), Expect = 1.746e-19
Identity = 9/20 (45.00%), Postives = 12/20 (60.00%), Query Frame = 3

Query:   21 SIMLWNAVIFGPDDTPWDGG 80
            ++M W   I G   TPW+GG
Sbjct:   37 NLMNWECAIPGKKGTPWEGG 56

BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBC9_RAT (SUMO-conjugating enzyme UBC9 OS=Rattus norvegicus GN=Ube2i PE=1 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.746e-19
Identity = 40/92 (43.48%), Postives = 57/92 (61.96%), Query Frame = 1

Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ--NQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVR 354
            FKL + F +DYP+ PP  +F   +FHPN+Y  G++CL IL+    W P   +  IL  IQ LL +PN   PA +EA  ++ +N+ EY +RVR
Sbjct:   58 FKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVR 149

HSP 2 Score: 26.1794 bits (56), Expect = 1.746e-19
Identity = 9/20 (45.00%), Postives = 12/20 (60.00%), Query Frame = 3

Query:   21 SIMLWNAVIFGPDDTPWDGG 80
            ++M W   I G   TPW+GG
Sbjct:   37 NLMNWECAIPGKKGTPWEGG 56

BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBC9_MOUSE (SUMO-conjugating enzyme UBC9 OS=Mus musculus GN=Ube2i PE=1 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.746e-19
Identity = 40/92 (43.48%), Postives = 57/92 (61.96%), Query Frame = 1

Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ--NQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVR 354
            FKL + F +DYP+ PP  +F   +FHPN+Y  G++CL IL+    W P   +  IL  IQ LL +PN   PA +EA  ++ +N+ EY +RVR
Sbjct:   58 FKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVR 149

HSP 2 Score: 26.1794 bits (56), Expect = 1.746e-19
Identity = 9/20 (45.00%), Postives = 12/20 (60.00%), Query Frame = 3

Query:   21 SIMLWNAVIFGPDDTPWDGG 80
            ++M W   I G   TPW+GG
Sbjct:   37 NLMNWECAIPGKKGTPWEGG 56

BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBC9_HUMAN (SUMO-conjugating enzyme UBC9 OS=Homo sapiens GN=UBE2I PE=1 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.746e-19
Identity = 40/92 (43.48%), Postives = 57/92 (61.96%), Query Frame = 1

Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ--NQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVR 354
            FKL + F +DYP+ PP  +F   +FHPN+Y  G++CL IL+    W P   +  IL  IQ LL +PN   PA +EA  ++ +N+ EY +RVR
Sbjct:   58 FKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVR 149

HSP 2 Score: 26.1794 bits (56), Expect = 1.746e-19
Identity = 9/20 (45.00%), Postives = 12/20 (60.00%), Query Frame = 3

Query:   21 SIMLWNAVIFGPDDTPWDGG 80
            ++M W   I G   TPW+GG
Sbjct:   37 NLMNWECAIPGKKGTPWEGG 56

BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBC9_CHICK (SUMO-conjugating enzyme UBC9 OS=Gallus gallus GN=UBE2I PE=2 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.746e-19
Identity = 40/92 (43.48%), Postives = 57/92 (61.96%), Query Frame = 1

Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ--NQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVR 354
            FKL + F +DYP+ PP  +F   +FHPN+Y  G++CL IL+    W P   +  IL  IQ LL +PN   PA +EA  ++ +N+ EY +RVR
Sbjct:   58 FKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVR 149

HSP 2 Score: 26.1794 bits (56), Expect = 1.746e-19
Identity = 9/20 (45.00%), Postives = 12/20 (60.00%), Query Frame = 3

Query:   21 SIMLWNAVIFGPDDTPWDGG 80
            ++M W   I G   TPW+GG
Sbjct:   37 NLMNWECAIPGKKGTPWEGG 56

BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBC9B_DANRE (SUMO-conjugating enzyme UBC9-B OS=Danio rerio GN=ube2ib PE=1 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.746e-19
Identity = 40/92 (43.48%), Postives = 57/92 (61.96%), Query Frame = 1

Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ--NQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVR 354
            FKL + F +DYP+ PP  +F   +FHPN+Y  G++CL IL+    W P   +  IL  IQ LL +PN   PA +EA  ++ +N+ EY +RVR
Sbjct:   58 FKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVR 149

HSP 2 Score: 26.1794 bits (56), Expect = 1.746e-19
Identity = 9/20 (45.00%), Postives = 12/20 (60.00%), Query Frame = 3

Query:   21 SIMLWNAVIFGPDDTPWDGG 80
            ++M W   I G   TPW+GG
Sbjct:   37 NLMNWECAIPGKKGTPWEGG 56

The following BLAST results are available for this feature:

BLAST of CK740326 vs. ExPASy Swiss-Prot

BLAST of CK740326 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 225


Match Name	E-value	Identity	Description
UBC31_ARATH	3.608e-20	41.76	Probable ubiquitin carrier protein E2 31 OS=Arabid...	[more]
UBC9_DICDI	7.935e-20	44.57	Sumo-conjugating enzyme ubc9 OS=Dictyostelium disc...	[more]
UBC9_XENTR	1.746e-19	43.48	SUMO-conjugating enzyme UBC9 OS=Xenopus tropicalis...	[more]
UBC9_XENLA	1.746e-19	43.48	SUMO-conjugating enzyme UBC9 OS=Xenopus laevis GN=...	[more]
UBC9_SPETR	1.746e-19	43.48	SUMO-conjugating enzyme UBC9 OS=Spermophilus tride...	[more]
UBC9_RAT	1.746e-19	43.48	SUMO-conjugating enzyme UBC9 OS=Rattus norvegicus ...	[more]
UBC9_MOUSE	1.746e-19	43.48	SUMO-conjugating enzyme UBC9 OS=Mus musculus GN=Ub...	[more]
UBC9_HUMAN	1.746e-19	43.48	SUMO-conjugating enzyme UBC9 OS=Homo sapiens GN=UB...	[more]
UBC9_CHICK	1.746e-19	43.48	SUMO-conjugating enzyme UBC9 OS=Gallus gallus GN=U...	[more]
UBC9B_DANRE	1.746e-19	43.48	SUMO-conjugating enzyme UBC9-B OS=Danio rerio GN=u...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_6470 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE63H07 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK740326 ID=CK740326; Name=CK740326; organism=Citrus sinensis; type=EST; length=463bp
GTGGTGCTCCTCAGGACAACAGTATAATGCTTTGGAATGCTGTTATATTC
GGGCCGGATGACACCCCATGGGATGGAGGTACCGTTTAAATTGACACTTC
AGTTCACAGAGGATTATCCAAATAAGCCACCAACAGTGCGCTTTGTTTCT
CGGATGTTCCATCCAAATATTTATGCGGATGGAAGCATTTGCTTGGACAT
TCTACAAAATCAGTGGAGTCCAATTTACGATGTAGCAGCAATACTCACGT
CAATTCAGTCATTGCTTTGTGACCCAAATCCAAACTCTCCTGCAAATTCG
GAGGCAGCACGGATGTTTAGCGAGAACAAGCGTGAATACCATAGGAGAGT
ACGGGAAATTGTTGAGCAGAGTTGGACTGCTGACTAGTGGCAACTTGTTG
GAATGTAGGGAACTTCAAAAATAGTCCAATGGCTTGTTCTAAATAACATT
TAAGATTCAAAAG