CK932833

Overview
NameCK932833
Unique NameCK932833
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length759
Libraries
Library NameType
Developing fruit juice sac at 38 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CK932833 vs. ExPASy Swiss-Prot
Match: PAL1_RUBID (Phenylalanine ammonia-lyase 1 OS=Rubus idaeus GN=PAL1 PE=2 SV=1)

HSP 1 Score: 412.92 bits (1060), Expect = 9.453e-115
Identity = 203/252 (80.56%), Postives = 223/252 (88.49%), Query Frame = 1
Query:    4 AMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKTAEAVDILKLMSSTFLVALCQAIDLRHLEENLKHTVKNTVSQVAKKVLTVGASGELHPSRFCEKDLLKAADREHVFAYIDDPCSATYPLMQKLRQVLVEHALNNGENEKTANSSIFQKIAAFEEELKTVLPKEVENARQTVENGSPTIPNRIKECRSYPLYRLVREELGSNFLTGEKVTSPGEEFDKVFTAMCQGKIIDPMLECLREWNGAP 759
            AMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKT+EAVDILKLMSSTFLVALCQAIDLRHLEENLK  VK TVS VAK+ LTV  +GELHPSRF EKDLL   DRE++F+YIDDPC ATYPLMQKLR  LVEHAL NGE E++AN+SIF KIAAFEEELKT+LPKEV+NAR  +ENG   IPNRIKECRSYPLYR VREELG++ LTGEK+ SPGEE  KVF A+C GK++DP+LECL+EWNGAP
Sbjct:  455 AMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKTSEAVDILKLMSSTFLVALCQAIDLRHLEENLKIVVKTTVSNVAKRTLTVSPNGELHPSRFSEKDLLTVVDREYLFSYIDDPCLATYPLMQKLRAELVEHALKNGERERSANTSIFHKIAAFEEELKTILPKEVDNARIEIENGKSEIPNRIKECRSYPLYRFVREELGTSLLTGEKIKSPGEECYKVFNAICAGKLVDPLLECLKEWNGAP 706          
BLAST of CK932833 vs. ExPASy Swiss-Prot
Match: PAL1_PHAVU (Phenylalanine ammonia-lyase class 1 (Fragment) OS=Phaseolus vulgaris PE=2 SV=2)

HSP 1 Score: 412.535 bits (1059), Expect = 1.235e-114
Identity = 200/252 (79.37%), Postives = 224/252 (88.89%), Query Frame = 1
Query:    4 AMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKTAEAVDILKLMSSTFLVALCQAIDLRHLEENLKHTVKNTVSQVAKKVLTVGASGELHPSRFCEKDLLKAADREHVFAYIDDPCSATYPLMQKLRQVLVEHALNNGENEKTANSSIFQKIAAFEEELKTVLPKEVENARQTVENGSPTIPNRIKECRSYPLYRLVREELGSNFLTGEKVTSPGEEFDKVFTAMCQGKIIDPMLECLREWNGAP 759
            AMASYCSELQ+LANPVT+HVQSAEQHNQDVNSL LIS+RKT E+++ILKLMSSTFL+ LCQAIDLRHLEENLK +VKNTVSQV+K+ LT G +GELHPSRFCEKDLLK  DRE+VF+YIDDP S TYPLMQKLRQVLV+HAL N ENEK  N+SIFQKIA FEEELKT+LPKEVE+ R   E+G   IPN+IKECRSYPLY+ VREELG+  LTGEKV SPGEEFDK+FTA+CQGKIIDP+LECL EWNGAP
Sbjct:  251 AMASYCSELQYLANPVTSHVQSAEQHNQDVNSLDLISARKTNESIEILKLMSSTFLMGLCQAIDLRHLEENLKSSVKNTVSQVSKRTLTTGGNGELHPSRFCEKDLLKVVDREYVFSYIDDPYSGTYPLMQKLRQVLVDHALINAENEKDVNTSIFQKIATFEEELKTILPKEVESTRAAYESGKAAIPNKIKECRSYPLYKFVREELGTGLLTGEKVKSPGEEFDKLFTAICQGKIIDPLLECLGEWNGAP 502          
BLAST of CK932833 vs. ExPASy Swiss-Prot
Match: PAL4_POPKI (Phenylalanine ammonia-lyase G4 (Fragment) OS=Populus kitakamiensis GN=PALG4 PE=3 SV=1)

HSP 1 Score: 411.379 bits (1056), Expect = 2.751e-114
Identity = 202/252 (80.16%), Postives = 228/252 (90.48%), Query Frame = 1
Query:    4 AMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKTAEAVDILKLMSSTFLVALCQAIDLRHLEENLKHTVKNTVSQVAKKVLTVGASGELHPSRFCEKDLLKAADREHVFAYIDDPCSATYPLMQKLRQVLVEHALNNGENEKTANSSIFQKIAAFEEELKTVLPKEVENARQTVENGSPTIPNRIKECRSYPLYRLVREELGSNFLTGEKVTSPGEEFDKVFTAMCQGKIIDPMLECLREWNGAP 759
            AMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLIS+RKTAEAV+IL +MS+T+LVALCQAIDLRH+EENLK+TVKNTVSQVAK+VLT+G +GELHPSRFCEKDLLK  DRE+VF YIDDPCSATYPLMQKLRQVLV+HAL NGE E+ +++SIFQKI AFEEELK +LPKEVE+AR  +ENG+P IPNRI + RSYPLY+ VREELG+  LTGEKV SPGEEFDKVFTA+C GK+IDP    L+EWNGAP
Sbjct:  316 AMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISARKTAEAVEILNVMSTTWLVALCQAIDLRHIEENLKNTVKNTVSQVAKRVLTMGFNGELHPSRFCEKDLLKVVDREYVFTYIDDPCSATYPLMQKLRQVLVDHALMNGEKEQNSSTSIFQKIGAFEEELKILLPKEVESARLELENGNPAIPNRITDRRSYPLYKFVREELGTVLLTGEKVGSPGEEFDKVFTAICAGKLIDPCWSVLKEWNGAP 567          
BLAST of CK932833 vs. ExPASy Swiss-Prot
Match: PAL2_POPKI (Phenylalanine ammonia-lyase G2B OS=Populus kitakamiensis GN=PALG2B PE=3 SV=1)

HSP 1 Score: 409.838 bits (1052), Expect = 8.003e-114
Identity = 200/252 (79.37%), Postives = 227/252 (90.08%), Query Frame = 1
Query:    4 AMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKTAEAVDILKLMSSTFLVALCQAIDLRHLEENLKHTVKNTVSQVAKKVLTVGASGELHPSRFCEKDLLKAADREHVFAYIDDPCSATYPLMQKLRQVLVEHALNNGENEKTANSSIFQKIAAFEEELKTVLPKEVENARQTVENGSPTIPNRIKECRSYPLYRLVREELGSNFLTGEKVTSPGEEFDKVFTAMCQGKIIDPMLECLREWNGAP 759
            AMASYCSELQFL    TNHVQSAEQHNQDVNSLGLISSRKTAEA+DILKLMS+TFLV LC ++DLRH+EENLK+TVK +VSQ+ + VLT+G +GELHPSRFCEKDLLK  DREHVF+YIDDPCSATYPLMQKLRQVLVEHAL NGE  + + +SIFQKI +FEEELKT+LPKEVE+AR  VENG+P IPNRIKECRSYPLY+ VREELG++ LTGEKV SPGEEFDKVFTA+C GK+IDP+LECL+EW+GAP
Sbjct:  456 AMASYCSELQFLDQSCTNHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSTTFLVGLCHSVDLRHIEENLKNTVKISVSQLPR-VLTMGFNGELHPSRFCEKDLLKVVDREHVFSYIDDPCSATYPLMQKLRQVLVEHALVNGEKVRNSTTSIFQKIGSFEEELKTLLPKEVESARLEVENGNPAIPNRIKECRSYPLYKFVREELGTSLLTGEKVKSPGEEFDKVFTAICAGKLIDPLLECLKEWDGAP 706          
BLAST of CK932833 vs. ExPASy Swiss-Prot
Match: PAL1_SOLLC (Phenylalanine ammonia-lyase OS=Solanum lycopersicum GN=PAL PE=2 SV=1)

HSP 1 Score: 408.297 bits (1048), Expect = 2.328e-113
Identity = 199/252 (78.97%), Postives = 224/252 (88.89%), Query Frame = 1
Query:    4 AMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKTAEAVDILKLMSSTFLVALCQAIDLRHLEENLKHTVKNTVSQVAKKVLTVGASGELHPSRFCEKDLLKAADREHVFAYIDDPCSATYPLMQKLRQVLVEHALNNGENEKTANSSIFQKIAAFEEELKTVLPKEVENARQTVENGSPTIPNRIKECRSYPLYRLVREELGSNFLTGEKVTSPGEEFDKVFTAMCQGKIIDPMLECLREWNGAP 759
            AMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLIS+RKTA+AVDILK+MSST+LVALCQAIDLRHLEENLK  VKNTVSQVAK+ LT+GA+GELHP+RF EK+LL+  DRE++FAY DDPCS+ YPLMQKLRQVLV+ A+ NGE+EK  NSSIFQKI AFE+EL  VLPKEVE+ R   E+G+P I NRI ECRSYPLYRLVREELG+  LTGEKV SPGEE DKVFTA+C G+IIDP+LECL+ WNGAP
Sbjct:  449 AMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISARKTAKAVDILKIMSSTYLVALCQAIDLRHLEENLKSVVKNTVSQVAKRTLTMGANGELHPARFSEKELLRVVDREYLFAYADDPCSSNYPLMQKLRQVLVDQAMKNGESEKNVNSSIFQKIGAFEDELIAVLPKEVESVRAVFESGNPLIRNRITECRSYPLYRLVREELGTELLTGEKVRSPGEEIDKVFTAICNGQIIDPLLECLKSWNGAP 700          
BLAST of CK932833 vs. ExPASy Swiss-Prot
Match: PAL1_LITER (Phenylalanine ammonia-lyase 1 OS=Lithospermum erythrorhizon PE=2 SV=1)

HSP 1 Score: 407.912 bits (1047), Expect = 3.041e-113
Identity = 198/252 (78.57%), Postives = 221/252 (87.70%), Query Frame = 1
Query:    4 AMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKTAEAVDILKLMSSTFLVALCQAIDLRHLEENLKHTVKNTVSQVAKKVLTVGASGELHPSRFCEKDLLKAADREHVFAYIDDPCSATYPLMQKLRQVLVEHALNNGENEKTANSSIFQKIAAFEEELKTVLPKEVENARQTVENGSPTIPNRIKECRSYPLYRLVREELGSNFLTGEKVTSPGEEFDKVFTAMCQGKIIDPMLECLREWNGAP 759
            AMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKT+EAV+ILKLMSS+FLVAL QA+DLRH+EEN++  VKNTVSQVAK+ LT G +GELHPSRF EKDLL+  DRE+VFAY DDPC  TYPLMQKLR+ LV HAL+NGENEK  N+SIF KIA FEEELK +LPKEVENAR +VENG P I NRI+ECRSYPLY+ VREELG+  LTGEKV SPGEE DKVFTAMC+GK++DP+L CL  WNGAP
Sbjct:  455 AMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKTSEAVEILKLMSSSFLVALFQAVDLRHIEENVRLAVKNTVSQVAKRTLTTGVNGELHPSRFSEKDLLRVVDREYVFAYADDPCLTTYPLMQKLRETLVGHALDNGENEKDVNTSIFHKIAIFEEELKAILPKEVENARASVENGIPAISNRIEECRSYPLYKFVREELGTELLTGEKVRSPGEELDKVFTAMCEGKLVDPLLACLEAWNGAP 706          
BLAST of CK932833 vs. ExPASy Swiss-Prot
Match: PAL2_LITER (Phenylalanine ammonia-lyase 2 OS=Lithospermum erythrorhizon PE=2 SV=1)

HSP 1 Score: 399.053 bits (1024), Expect = 1.413e-110
Identity = 192/252 (76.19%), Postives = 219/252 (86.90%), Query Frame = 1
Query:    4 AMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKTAEAVDILKLMSSTFLVALCQAIDLRHLEENLKHTVKNTVSQVAKKVLTVGASGELHPSRFCEKDLLKAADREHVFAYIDDPCSATYPLMQKLRQVLVEHALNNGENEKTANSSIFQKIAAFEEELKTVLPKEVENARQTVENGSPTIPNRIKECRSYPLYRLVREELGSNFLTGEKVTSPGEEFDKVFTAMCQGKIIDPMLECLREWNGAP 759
            AMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKT+EAV+ILKLMSS+FLVALCQA+DLRH+EEN++  VK TVSQVAKK L +G  G LHPSRF EK+LL+  DRE+VFAY DDPCSATYPLMQKLR+VLV HAL N  NEK A++SIF KI  FEEELK +LPKEVENAR +VENG+P IPN+I+ECRSYPLY+ VR ELG+  LTGEKV SPGEE D+VF A+C+GK++DP+L CL  WNGAP
Sbjct:  450 AMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKTSEAVEILKLMSSSFLVALCQAVDLRHIEENVRLAVKKTVSQVAKKTLNIGVDGVLHPSRFSEKELLRVVDREYVFAYADDPCSATYPLMQKLREVLVSHALANSGNEKDASTSIFHKIGVFEEELKGILPKEVENARASVENGTPAIPNKIEECRSYPLYKFVRGELGTELLTGEKVRSPGEELDQVFNALCEGKLVDPLLACLEAWNGAP 701          
BLAST of CK932833 vs. ExPASy Swiss-Prot
Match: PAL4_ARATH (Phenylalanine ammonia-lyase 4 OS=Arabidopsis thaliana GN=PAL4 PE=1 SV=1)

HSP 1 Score: 387.882 bits (995), Expect = 3.257e-107
Identity = 190/252 (75.40%), Postives = 215/252 (85.32%), Query Frame = 1
Query:    4 AMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKTAEAVDILKLMSSTFLVALCQAIDLRHLEENLKHTVKNTVSQVAKKVLTVGASGELHPSRFCEKDLLKAADREHVFAYIDDPCSATYPLMQKLRQVLVEHALNNGENEKTANSSIFQKIAAFEEELKTVLPKEVENARQTVENGSPTIPNRIKECRSYPLYRLVREELGSNFLTGEKVTSPGEEFDKVFTAMCQGKIIDPMLECLREWNGAP 759
            AMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKTAEAVDILKLMS+T+LVALCQA+DLRHLEENLK  VK+ VSQVAK+VLTVGA+GELHPSRF E+D+L+  DRE+VF+Y DDPCS TYPLMQKLR +LV+HAL + E E  + +S+F KI AFE ELK +LPKEVE  R   E G+  I NRIKECRSYPLYR VR+EL +  LTGE V SPGEEFDKVF A+  GK+IDP+LECL+EWNGAP
Sbjct:  452 AMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKTAEAVDILKLMSTTYLVALCQAVDLRHLEENLKKAVKSAVSQVAKRVLTVGANGELHPSRFTERDVLQVVDREYVFSYADDPCSLTYPLMQKLRHILVDHALADPEREANSATSVFHKIGAFEAELKLLLPKEVERVRVEYEEGTSAIANRIKECRSYPLYRFVRDELNTELLTGENVRSPGEEFDKVFLAISDGKLIDPLLECLKEWNGAP 703          
BLAST of CK932833 vs. ExPASy Swiss-Prot
Match: PALY_MALDO (Phenylalanine ammonia-lyase (Fragment) OS=Malus domestica GN=PAL PE=2 SV=1)

HSP 1 Score: 382.104 bits (980), Expect = 1.787e-105
Identity = 185/229 (80.79%), Postives = 205/229 (89.52%), Query Frame = 1
Query:   73 EQHNQDVNSLGLISSRKTAEAVDILKLMSSTFLVALCQAIDLRHLEENLKHTVKNTVSQVAKKVLTVGASGELHPSRFCEKDLLKAADREHVFAYIDDPCSATYPLMQKLRQVLVEHALNNGENEKTANSSIFQKIAAFEEELKTVLPKEVENARQTVENGSPTIPNRIKECRSYPLYRLVREELGSNFLTGEKVTSPGEEFDKVFTAMCQGKIIDPMLECLREWNGAP 759
            EQHNQDVNSLGLISSRKTAEAVDILKLMSSTFLVALCQ+IDLRHLEENL++TVKNTVSQVAK+ LT G +GELHPSRFCEKDLLK  DRE+VFAYIDDPCSATYPLMQKLR+VL+EHAL NGE+EK A++SIFQKI AFEEELK +LPKEVE+AR  +E G+  +PNRI ECRSYPLY+ VREELG  +LTGEKV SPGEE DKVF A+CQGKIIDP+L CL  WNGAP
Sbjct:    3 EQHNQDVNSLGLISSRKTAEAVDILKLMSSTFLVALCQSIDLRHLEENLRNTVKNTVSQVAKRTLTTGVNGELHPSRFCEKDLLKVVDREYVFAYIDDPCSATYPLMQKLREVLIEHALTNGESEKNASTSIFQKIGAFEEELKALLPKEVESARSAIEGGNAAVPNRIAECRSYPLYKFVREELGGEYLTGEKVRSPGEECDKVFQAICQGKIIDPILGCLEGWNGAP 231          
BLAST of CK932833 vs. ExPASy Swiss-Prot
Match: PALY_WHEAT (Phenylalanine ammonia-lyase OS=Triticum aestivum GN=PAL PE=3 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 3.726e-103
Identity = 187/252 (74.21%), Postives = 207/252 (82.14%), Query Frame = 1
Query:    4 AMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKTAEAVDILKLMSSTFLVALCQAIDLRHLEENLKHTVKNTVSQVAKKVLTVGASGELHPSRFCEKDLLKAADREHVFAYIDDPCSATYPLMQKLRQVLVEHALNNGENEKTANSSIFQKIAAFEEELKTVLPKEVENARQTVENGSPTIPNRIKECRSYPLYRLVREELGSNFLTGEKVTSPGEEFDKVFTAMCQGKIIDPMLECLREWNGAP 759
            AMASYCSELQFL NPVTNHVQSAEQHNQDVNSLGLISSRKTAEA+DILKLMSSTFLVALCQAIDLRHLEEN+K+ VK+ V  VA+K L+   +G LH +RFCEKDLL   DRE VFAY DDPCSA YPLMQK+R VLVEHAL NGE E    +S+F K+A FE+EL+ VLPKEVE AR  VENG+    NRI ECRSYPLYR VR+ELG+ +LTGEK  SPGEE DKVF AM QGK ID +LECL+EWNG P
Sbjct:  445 AMASYCSELQFLGNPVTNHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENVKNAVKSCVKTVARKTLSTDNNGHLHNARFCEKDLLLTIDREAVFAYADDPCSANYPLMQKMRAVLVEHALANGEAEAHVETSVFAKLAMFEQELRAVLPKEVEAARSAVENGTAAQQNRIAECRSYPLYRFVRKELGTEYLTGEKTRSPGEEVDKVFVAMNQGKHIDALLECLKEWNGEP 696          
The following BLAST results are available for this feature:
BLAST of CK932833 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 52
Match NameE-valueIdentityDescription
PAL1_RUBID9.453e-11580.56Phenylalanine ammonia-lyase 1 OS=Rubus idaeus GN=P... [more]
PAL1_PHAVU1.235e-11479.37Phenylalanine ammonia-lyase class 1 (Fragment) OS=... [more]
PAL4_POPKI2.751e-11480.16Phenylalanine ammonia-lyase G4 (Fragment) OS=Popul... [more]
PAL2_POPKI8.003e-11479.37Phenylalanine ammonia-lyase G2B OS=Populus kitakam... [more]
PAL1_SOLLC2.328e-11378.97Phenylalanine ammonia-lyase OS=Solanum lycopersicu... [more]
PAL1_LITER3.041e-11378.57Phenylalanine ammonia-lyase 1 OS=Lithospermum eryt... [more]
PAL2_LITER1.413e-11076.19Phenylalanine ammonia-lyase 2 OS=Lithospermum eryt... [more]
PAL4_ARATH3.257e-10775.40Phenylalanine ammonia-lyase 4 OS=Arabidopsis thali... [more]
PALY_MALDO1.787e-10580.79Phenylalanine ammonia-lyase (Fragment) OS=Malus do... [more]
PALY_WHEAT3.726e-10374.21Phenylalanine ammonia-lyase OS=Triticum aestivum G... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004350_H01 Developing fruit juice sac at 38 DAFB Citrus sinensis cDNA clone JSJune0004_IVF_H01 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK932833 ID=CK932833; Name=CK932833; organism=Citrus sinensis; type=EST; length=759bp
GGGGCTATGGCTTCATATTGTTCCGAGCTCCAATTTCTTGCCAATCCTGT
TACTAACCACGTCCAAAGTGCTGAACAGCACAACCAAGATGTAAACTCCT
TGGGATTGATCTCTTCCAGGAAGACTGCTGAAGCAGTCGACATCCTGAAG
CTCATGTCTTCCACATTCTTGGTAGCGCTTTGCCAGGCTATTGATTTGAG
ACATTTGGAGGAGAATTTGAAGCATACAGTCAAGAATACTGTCAGCCAAG
TAGCTAAGAAAGTCTTAACCGTCGGTGCTAGTGGAGAGCTTCATCCATCA
AGATTCTGTGAGAAGGATCTGCTCAAAGCGGCTGATCGCGAACACGTCTT
TGCATACATTGATGACCCCTGCAGTGCTACCTATCCATTGATGCAAAAGC
TAAGGCAAGTACTTGTTGAGCACGCACTGAACAATGGAGAGAACGAGAAG
ACTGCCAACTCTTCAATCTTCCAGAAGATTGCAGCCTTTGAGGAGGAATT
GAAAACCGTTTTGCCCAAAGAAGTTGAGAACGCCAGGCAGACTGTTGAGA
ATGGAAGTCCAACAATTCCCAACAGGATCAAAGAATGCAGGTCTTACCCT
TTGTACAGGTTAGTGAGGGAGGAGCTCGGGTCTAATTTTCTAACAGGGGA
AAAAGTTACATCGCCCGGCGAGGAATTTGACAAAGTGTTTACAGCAATGT
GCCAGGGCAAGATCATTGATCCAATGCTGGAATGTCTCAGGGAATGGAAT
GGCGCCCCT
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