CK932932 | Citrus Genome Database

Overview


Name	CK932932
Unique Name	CK932932
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	699

Libraries


Library Name	Type
Developing fruit juice sac at 38 DAFB	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
CK932932_ssr83	microsatellite	CK932932_ssr83:83..109.	BLAST: Citrus ESTs to Prunus persica proteins V1
CK932932_ssr105	microsatellite	CK932932_ssr105:105..116.	BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig9730	contig	Csv1_Contig9730:1..693.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CK932932 vs. ExPASy Swiss-Prot
Match: PIP11_ORYSJ (Aquaporin PIP1-1 OS=Oryza sativa subsp. japonica GN=PIP1-1 PE=2 SV=1)

HSP 1 Score: 231.876 bits (590), Expect = 5.585e-65
Identity = 111/158 (70.25%), Postives = 125/158 (79.11%), Query Frame = 2

Query:  167 KDYVDPPPAPLIDMAELKLWSFYRALIAEFVATLLFLYVSVATVIGHKKQSDACGGVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAVAFGLFLARKVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTF 640
            KDY +PPPAPL +  ELK WSFYRA IAEFVAT LFLY+++ TV+G  K S  C  VG+ GIAW+FGGMIF LVYCTAGISGGHINPAV FGLFLARK+SL RA+ Y+V QCLGAICG G+VK F +  Y   GGGAN VASGY KG  LGAEI+GTF
Sbjct:   32 KDYKEPPPAPLFEPGELKSWSFYRAGIAEFVATFLFLYITILTVMGVSKSSSKCATVGIQGIAWSFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAIFYIVMQCLGAICGAGVVKGFQQGLYMGNGGGANVVASGYTKGDGLGAEIVGTF 189

HSP 2 Score: 38.1206 bits (87), Expect = 5.585e-65
Identity = 17/20 (85.00%), Postives = 19/20 (95.00%), Query Frame = 3

Query:  639 FVLVYTVFSATDPKRSARDS 698
            F+LVYTVFSATD KR+ARDS
Sbjct:  189 FILVYTVFSATDAKRNARDS 208

[Close]

BLAST of CK932932 vs. ExPASy Swiss-Prot
Match: PIP12_ORYSJ (Probable aquaporin PIP1-2 OS=Oryza sativa subsp. japonica GN=PIP1-2 PE=2 SV=3)

HSP 1 Score: 230.335 bits (586), Expect = 1.615e-64
Identity = 110/158 (69.62%), Postives = 124/158 (78.48%), Query Frame = 2

Query:  167 KDYVDPPPAPLIDMAELKLWSFYRALIAEFVATLLFLYVSVATVIGHKKQSDACGGVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAVAFGLFLARKVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTF 640
            KDY +PPPAPL +  ELK WSFYRA IAEF+AT LFLY++V TV+G    +  C  VG+ GIAW+FGGMIF LVYCTAGISGGHINPAV FGLFLARK+SL RA+ YMV QCLGAICG G+VK F K  Y + GGGAN VA GY KG  LGAEI+GTF
Sbjct:   31 KDYKEPPPAPLFEPGELKSWSFYRAGIAEFMATFLFLYITVLTVMGVNNSTSKCATVGIQGIAWSFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRALFYMVMQCLGAICGAGVVKGFQKGLYETTGGGANVVAPGYTKGDGLGAEIVGTF 188

HSP 2 Score: 38.1206 bits (87), Expect = 1.615e-64
Identity = 17/20 (85.00%), Postives = 19/20 (95.00%), Query Frame = 3

Query:  639 FVLVYTVFSATDPKRSARDS 698
            F+LVYTVFSATD KR+ARDS
Sbjct:  188 FILVYTVFSATDAKRNARDS 207

[Close]

BLAST of CK932932 vs. ExPASy Swiss-Prot
Match: PIP15_MAIZE (Aquaporin PIP1-5 OS=Zea mays GN=PIP1-5 PE=2 SV=1)

HSP 1 Score: 228.024 bits (580), Expect = 2.746e-64
Identity = 110/158 (69.62%), Postives = 122/158 (77.22%), Query Frame = 2

Query:  167 KDYVDPPPAPLIDMAELKLWSFYRALIAEFVATLLFLYVSVATVIGHKKQSDACGGVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAVAFGLFLARKVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTF 640
            KDY +PPPAPL +  EL  WSFYRA IAEFVAT LFLY+S+ TV+G  K S  C  VG+ GIAW+FGGMIF LVYCTAGISGGHINPAV FGLFLARK+SL RA+ YMV QCLGAICG G+VK F +  Y   GGGAN V  GY KG  LGAEI+GTF
Sbjct:   31 KDYKEPPPAPLFEAEELTSWSFYRAGIAEFVATFLFLYISILTVMGVSKSSSKCATVGIQGIAWSFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRALFYMVMQCLGAICGAGVVKGFQEGLYMGAGGGANAVNPGYTKGDGLGAEIVGTF 188

HSP 2 Score: 39.6614 bits (91), Expect = 2.746e-64
Identity = 19/20 (95.00%), Postives = 19/20 (95.00%), Query Frame = 3

Query:  639 FVLVYTVFSATDPKRSARDS 698
            FVLVYTVFSATD KRSARDS
Sbjct:  188 FVLVYTVFSATDAKRSARDS 207

[Close]

BLAST of CK932932 vs. ExPASy Swiss-Prot
Match: PIP13_MAIZE (Aquaporin PIP1-3/PIP1-4 OS=Zea mays GN=PIP1-3 PE=2 SV=1)

HSP 1 Score: 228.794 bits (582), Expect = 4.668e-64
Identity = 109/158 (68.99%), Postives = 124/158 (78.48%), Query Frame = 2

Query:  167 KDYVDPPPAPLIDMAELKLWSFYRALIAEFVATLLFLYVSVATVIGHKKQSDACGGVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAVAFGLFLARKVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTF 640
            KDY +PPPAPL +  ELK WSFYRA IAEFVAT LFLY++V TV+G  K +  C  VG+ GIAW+FGGMIF LVYCTAGISGGHINPAV FGLFLARK+SL RA+ Y++ QCLGAICG G+VK F +  Y   GGGAN VA GY KG  LGAEI+GTF
Sbjct:   35 KDYKEPPPAPLFEPGELKSWSFYRAGIAEFVATFLFLYITVLTVMGVSKSTSKCATVGIQGIAWSFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFQQGLYMGNGGGANVVAPGYTKGDGLGAEIVGTF 192

HSP 2 Score: 38.1206 bits (87), Expect = 4.668e-64
Identity = 17/20 (85.00%), Postives = 19/20 (95.00%), Query Frame = 3

Query:  639 FVLVYTVFSATDPKRSARDS 698
            F+LVYTVFSATD KR+ARDS
Sbjct:  192 FILVYTVFSATDAKRNARDS 211

[Close]

BLAST of CK932932 vs. ExPASy Swiss-Prot
Match: PIP16_MAIZE (Aquaporin PIP1-6 OS=Zea mays GN=PIP1-6 PE=2 SV=1)

HSP 1 Score: 228.024 bits (580), Expect = 6.085e-64
Identity = 109/159 (68.55%), Postives = 124/159 (77.99%), Query Frame = 2

Query:  164 GKDYVDPPPAPLIDMAELKLWSFYRALIAEFVATLLFLYVSVATVIGHKKQSDACGGVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAVAFGLFLARKVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTF 640
            G+DY +PP APL + +EL  WSFYRA IAEFVAT LFLYV+V TV+G  K    CG VG+ GIAWAFGGMIF LVYCTAG+SGGHINPAV FGL LARK+SL RAV Y+V QCLGA+CG G+VKAF    Y S GGGAN V+ GY KG  LGAE++GTF
Sbjct:   36 GRDYSEPPAAPLFEASELSSWSFYRAGIAEFVATFLFLYVTVLTVMGVSKSPSKCGTVGIQGIAWAFGGMIFALVYCTAGVSGGHINPAVTFGLLLARKLSLTRAVYYVVMQCLGAVCGAGVVKAFGSALYESAGGGANAVSPGYTKGDGLGAEVVGTF 194

HSP 2 Score: 38.5058 bits (88), Expect = 6.085e-64
Identity = 18/20 (90.00%), Postives = 19/20 (95.00%), Query Frame = 3

Query:  639 FVLVYTVFSATDPKRSARDS 698
            FVLVYTVFSATD KR+ARDS
Sbjct:  194 FVLVYTVFSATDAKRTARDS 213

[Close]

BLAST of CK932932 vs. ExPASy Swiss-Prot
Match: PIP12_MAIZE (Aquaporin PIP1-2 OS=Zea mays GN=PIP1-2 PE=1 SV=1)

HSP 1 Score: 227.254 bits (578), Expect = 1.350e-63
Identity = 107/158 (67.72%), Postives = 124/158 (78.48%), Query Frame = 2

Query:  167 KDYVDPPPAPLIDMAELKLWSFYRALIAEFVATLLFLYVSVATVIGHKKQSDACGGVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAVAFGLFLARKVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTF 640
            KDY +PPPAPL +  ELK WSFYRA IAEFVAT LFLY+++ TV+G  K +  C  VG+ GIAW+FGGMIF LVYCTAGISGGHINPAV FGLFLARK+SL RA+ Y++ QCLGA+CG G+VK F +  Y   GGGAN VA GY KG  LGAEI+GTF
Sbjct:   32 KDYKEPPPAPLFEPGELKSWSFYRAGIAEFVATFLFLYITILTVMGVSKSTSKCATVGIQGIAWSFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRALFYIIMQCLGAVCGAGVVKGFQQGLYMGNGGGANVVAPGYTKGDGLGAEIVGTF 189

HSP 2 Score: 38.1206 bits (87), Expect = 1.350e-63
Identity = 17/20 (85.00%), Postives = 19/20 (95.00%), Query Frame = 3

Query:  639 FVLVYTVFSATDPKRSARDS 698
            F+LVYTVFSATD KR+ARDS
Sbjct:  189 FILVYTVFSATDAKRNARDS 208

[Close]

BLAST of CK932932 vs. ExPASy Swiss-Prot
Match: PIP13_ORYSJ (Aquaporin PIP 1-3 OS=Oryza sativa subsp. japonica GN=PIP1-3 PE=2 SV=2)

HSP 1 Score: 222.631 bits (566), Expect = 2.500e-62
Identity = 107/158 (67.72%), Postives = 121/158 (76.58%), Query Frame = 2

Query:  167 KDYVDPPPAPLIDMAELKLWSFYRALIAEFVATLLFLYVSVATVIGHKKQSDACGGVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAVAFGLFLARKVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTF 640
            KDY +PP AP+ ++ EL  WSFYRA IAEFVAT LFLY+S+ TV+G  K +  C  VG+ GIAW+FGGMIF LVYCTAGISGGHINPAV FGLFLARK+SL RAV YM  QCLGAICG G+VK F +  Y   GGGAN V  GY KG  LGAEI+GTF
Sbjct:   31 KDYREPPAAPVFEVEELTSWSFYRAGIAEFVATFLFLYISILTVMGVNKSASKCATVGIQGIAWSFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAVFYMAMQCLGAICGAGVVKGFQRGLYMGSGGGANAVNPGYTKGDGLGAEIVGTF 188

HSP 2 Score: 38.5058 bits (88), Expect = 2.500e-62
Identity = 18/20 (90.00%), Postives = 19/20 (95.00%), Query Frame = 3

Query:  639 FVLVYTVFSATDPKRSARDS 698
            FVLVYTVFSATD KR+ARDS
Sbjct:  188 FVLVYTVFSATDAKRNARDS 207

[Close]

BLAST of CK932932 vs. ExPASy Swiss-Prot
Match: PIP2_PEA (Probable aquaporin PIP-type 7a OS=Pisum sativum GN=TRG-31 PE=2 SV=2)

HSP 1 Score: 221.861 bits (564), Expect = 2.500e-62
Identity = 109/159 (68.55%), Postives = 124/159 (77.99%), Query Frame = 2

Query:  167 KDYVDPPPAPLIDMAELKLWSFYRALIAEFVATLLFLYVSVATVIGHKKQSDACGGVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAVAFGLFLARKVSLIRAVAYMVAQCLGAICGVGLVKAFM-KHEYNSLGGGANTVASGYNKGSALGAEIIGTF 640
            KDY +PPPAPL + +EL  WSFYRA IAEF+AT LFLY++V TV+G  ++S  C  VG+ GIAWAFGGMIF LVYCTAGISGGHINPAV FGLFLARK+SL RA+ YMV Q LGAICG G+VK F  K  +  L GGAN VA GY KG  LGAEI+GTF
Sbjct:   32 KDYQEPPPAPLFEPSELTSWSFYRAGIAEFIATFLFLYITVLTVMGVVRESSKCKTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAIFYMVMQVLGAICGAGVVKGFEGKQRFGDLNGGANFVAPGYTKGDGLGAEIVGTF 190

HSP 2 Score: 39.2762 bits (90), Expect = 2.500e-62
Identity = 18/20 (90.00%), Postives = 19/20 (95.00%), Query Frame = 3

Query:  639 FVLVYTVFSATDPKRSARDS 698
            F+LVYTVFSATD KRSARDS
Sbjct:  190 FILVYTVFSATDAKRSARDS 209

[Close]

BLAST of CK932932 vs. ExPASy Swiss-Prot
Match: PIP11_MAIZE (Aquaporin PIP1-1 OS=Zea mays GN=PIP1-1 PE=1 SV=1)

HSP 1 Score: 212.616 bits (540), Expect = 5.491e-59
Identity = 105/158 (66.46%), Postives = 119/158 (75.32%), Query Frame = 2

Query:  167 KDYVDPPPAPLIDMAELKLWSFYRALIAEFVATLLFLYVSVATVIGHKKQSDACGGVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAVAFGLFLARKVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTF 640
            KDY +PPPAPL +  ELK WSFYR  IAEFVAT LFLY+S+ TV+G  K +  C  VG+ GIAW+FGGMI  LVYCTAGIS GHINPAV FGLFLARK+SL RAV Y++ QCLGAICG G+VK F +  Y   GG  N VA GY KG  LGAEI+GTF
Sbjct:   31 KDYKEPPPAPLFEPGELKSWSFYRPGIAEFVATFLFLYISILTVMGVSKSTSKCATVGIQGIAWSFGGMILALVYCTAGIS-GHINPAVTFGLFLARKLSLTRAVFYIIMQCLGAICGRGVVKGFQQGLYMGNGGRRNVVAPGYTKGDGLGAEIVGTF 187

HSP 2 Score: 37.3502 bits (85), Expect = 5.491e-59
Identity = 17/20 (85.00%), Postives = 18/20 (90.00%), Query Frame = 3

Query:  639 FVLVYTVFSATDPKRSARDS 698
            F+LVYTVFSATD KR ARDS
Sbjct:  187 FILVYTVFSATDAKRRARDS 206

[Close]

BLAST of CK932932 vs. ExPASy Swiss-Prot
Match: PIP28_ORYSJ (Probable aquaporin PIP2-8 OS=Oryza sativa subsp. japonica GN=PIP2-8 PE=2 SV=1)

HSP 1 Score: 204.527 bits (519), Expect = 5.979e-58
Identity = 101/159 (63.52%), Postives = 123/159 (77.36%), Query Frame = 2

Query:  167 KDYVDPPPAPLIDMAELKLWSFYRALIAEFVATLLFLYVSVATVIGHKKQSDACGGVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAVAFGLFLARKVSLIRAVAYMVAQCLGAICGVGLVKAFM-KHEYNSLGGGANTVASGYNKGSALGAEIIGTF 640
            KDY DPPPAPL+D  EL  WS YRA IAEF ATLL + +SV+TVIG K+QS   GG G+LGIAWAFGG+IFVLVYCTAGISGGH+NPAV F + LAR+VSL RA  Y +AQC+GA+CG GL +A     +Y   GGGAN +A+GY+ G+ + AE++GTF
Sbjct:   12 KDYQDPPPAPLVDTGELGKWSLYRAAIAEFTATLLLVCISVSTVIGEKRQSGE-GGAGVLGIAWAFGGLIFVLVYCTAGISGGHMNPAVTFAMVLARRVSLPRAALYTMAQCVGAVCGAGLARAMHGGGQYARHGGGANELAAGYSAGAGVVAEMVGTF 169

HSP 2 Score: 41.9726 bits (97), Expect = 5.979e-58
Identity = 20/27 (74.07%), Postives = 22/27 (81.48%), Query Frame = 3

Query:  618 VLRLLVPFVLVYTVFSATDPKRSARDS 698
            V  ++  FVLVYTVFSATDPKR ARDS
Sbjct:  162 VAEMVGTFVLVYTVFSATDPKRKARDS 188

The following BLAST results are available for this feature:

BLAST of CK932932 vs. ExPASy Swiss-Prot

BLAST of CK932932 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 124


Match Name	E-value	Identity	Description
PIP11_ORYSJ	5.585e-65	70.25	Aquaporin PIP1-1 OS=Oryza sativa subsp. japonica G...	[more]
PIP12_ORYSJ	1.615e-64	69.62	Probable aquaporin PIP1-2 OS=Oryza sativa subsp. j...	[more]
PIP15_MAIZE	2.746e-64	69.62	Aquaporin PIP1-5 OS=Zea mays GN=PIP1-5 PE=2 SV=1	[more]
PIP13_MAIZE	4.668e-64	68.99	Aquaporin PIP1-3/PIP1-4 OS=Zea mays GN=PIP1-3 PE=2...	[more]
PIP16_MAIZE	6.085e-64	68.55	Aquaporin PIP1-6 OS=Zea mays GN=PIP1-6 PE=2 SV=1	[more]
PIP12_MAIZE	1.350e-63	67.72	Aquaporin PIP1-2 OS=Zea mays GN=PIP1-2 PE=1 SV=1	[more]
PIP13_ORYSJ	2.500e-62	67.72	Aquaporin PIP 1-3 OS=Oryza sativa subsp. japonica ...	[more]
PIP2_PEA	2.500e-62	68.55	Probable aquaporin PIP-type 7a OS=Pisum sativum GN...	[more]
PIP11_MAIZE	5.491e-59	66.46	Aquaporin PIP1-1 OS=Zea mays GN=PIP1-1 PE=1 SV=1	[more]
PIP28_ORYSJ	5.979e-58	63.52	Probable aquaporin PIP2-8 OS=Oryza sativa subsp. j...	[more]

Pages

Properties


Property Name	Value
Genbank description	CGF1004349_F09 Developing fruit juice sac at 38 DAFB Citrus sinensis cDNA clone JSJune0004_IIIF_F09 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK932932 ID=CK932932; Name=CK932932; organism=Citrus sinensis; type=EST; length=699bp
GGGGACAGACGCTACCTTTTCTCGAGACCAGAGAGGGTGTGTGCTTTTCT
TTGATTGTTCCAAAGAGAAATCGAAATCTTGTGAAGAAGAAGAAGAAGAA
GAAGAAGAAAGAAAGATGTCAAAGGAGGTGAATGAAGAAGGACAAACTCA
CCGCCATCACCATGGCAAGGACTACGTGGACCCACCACCAGCACCACTCA
TTGACATGGCTGAGCTCAAGCTCTGGTCCTTTTACAGAGCTCTCATAGCC
GAGTTCGTGGCCACTCTCCTCTTCCTCTACGTCAGTGTTGCCACTGTCAT
TGGCCACAAGAAACAAAGTGATGCCTGTGGTGGTGTTGGCCTTTTGGGTA
TTGCATGGGCCTTTGGTGGCATGATCTTCGTCCTTGTTTACTGCACCGCT
GGCATTTCTGGTGGTCACATTAACCCTGCTGTGGCGTTTGGGCTGTTCTT
GGCAAGGAAGGTATCATTGATCAGGGCTGTGGCTTACATGGTGGCTCAGT
GCTTGGGTGCCATTTGCGGTGTTGGTTTGGTCAAGGCCTTCATGAAGCAT
GAGTACAACTCACTTGGTGGTGGTGCTAACACTGTGGCTTCTGGCTACAA
CAAAGGATCAGCCTTGGGTGCTGAGATTATTGGTACCTTTCGTGCTTGTT
TACACAGTCTTCTCGGCCACAGACCCCAAGAGAAGCGCTCGTGACTCTC