CK933105

Overview
NameCK933105
Unique NameCK933105
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length797
Libraries
Library NameType
Developing fruit juice sac at 38 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CK933105_ssr43 microsatellite CK933105_ssr43:43..60. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig8025 contig Csv1_Contig8025:1..786. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
BLAST of CK933105 vs. ExPASy Swiss-Prot
Match: RS4_SULIA (30S ribosomal protein S4P OS=Sulfolobus islandicus (strain M.16.27) GN=rps4p PE=3 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 2.402e-23
Identity = 60/160 (37.50%), Postives = 93/160 (58.13%), Query Frame = 1
Query:  121 KTFKKPRRPYEKERLDAELRLVGEYGLRCKRELWRVQYALSRIRNAARDLLTLDEKNPRRIFEGEALLRRMHRYGFLEEGQNKLDYVLALTVENFLERRLQTLVFKSGMAKSIHHARVLIRQRHIRVGRQLVNIPSFVVRVDSQKHIEFSLTSPFGGGRP 600
            K ++ P  P+ KER+  E  L+G+YGLR KRE+W  Q  + + R+ AR LL L      R    + L+ ++ + G L+     +D +L+LT ++ LERRLQT+V+K G+A + + AR LI   HI V  + V  P ++V VD +  I++ +TS F    P
Sbjct:    9 KKWESPGHPWIKERIGYEQELLGKYGLRNKREIWIAQSIIRKFRHQARSLLALPPAE--RAVREKQLVGKLLKMGLLKRETATVDDILSLTEQDLLERRLQTIVYKKGLANTTYQARQLIIHGHIAVNGKRVTSPGYIVNVDEENLIDYYVTSSFKSRPP 166          
BLAST of CK933105 vs. ExPASy Swiss-Prot
Match: RS4_KORCO (30S ribosomal protein S4P OS=Korarchaeum cryptofilum (strain OPF8) GN=rps4p PE=3 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 2.402e-23
Identity = 58/152 (38.16%), Postives = 93/152 (61.18%), Query Frame = 1
Query:  109 RNYGKTFKKPRRPYEKERLDAELRLVGEYGLRCKRELWRVQYALSRIRNAARDLLTLDEKNPRRIFEGEALLRRMHRYGFLEEGQNKLDYVLALTVENFLERRLQTLVFKSGMAKSIHHARVLIRQRHIRVGRQLVNIPSFVVRVDSQKHIE 564
            R + K +++P +P+++  L+   RL G YGLR KRELWR+ +   + R  AR LL   +    ++   E +++++   G L +    LD +L L+VE FLERRLQT+V++ G AKS + AR LI   HIRV  + +  PS++V+++ +  IE
Sbjct:    5 RKHRKKYERPYKPWDRRVLEETNRLAGYYGLRNKRELWRMSFLAKKYRRIARQLLAAPKSESWKV---EPIIKKLQALGILSKDAT-LDDLLDLSVEQFLERRLQTIVWRKGFAKSPYMARQLITHGHIRVNGRRIRQPSYLVKIEEEDKIE 152          
BLAST of CK933105 vs. ExPASy Swiss-Prot
Match: RS4_THEVO (30S ribosomal protein S4P OS=Thermoplasma volcanium GN=rps4p PE=3 SV=1)

HSP 1 Score: 108.997 bits (271), Expect = 3.138e-23
Identity = 64/157 (40.76%), Postives = 95/157 (60.51%), Query Frame = 1
Query:  121 KTFKKPRRPYEKERLDAELRLVGEYGLRCKRELWRVQYALSRIRNAARDL---LTLDEKNPRRIFEGEALLRRMHRYGFLEEGQNKLDYVLALTVENFLERRLQTLVFKSGMAKSIHHARVLIRQRHIRVGRQLVNIPSFVVRVDSQKHIEFSLTSP 582
            K +  PR P+EK+R+D E +++ +YGL+ KRE+WR +  LS IR+ AR L   L   + N ++  + E +++R+ RY  L + +  LD VL+LTVEN L+RRLQT+VFK  +A S   AR LI   HI V  + V +P  +V    +  I +   SP
Sbjct:    9 KKYSTPRHPWEKDRIDEENKILVKYGLKNKREIWRSEAMLSSIRSQARYLRARLRASDANAQK--QLERMIKRLSRYKILSD-KATLDDVLSLTVENILDRRLQTIVFKKNLALSEKQARQLITHGHITVNGRRVTVPGMLVEAQYEDTIAYYENSP 162          
BLAST of CK933105 vs. ExPASy Swiss-Prot
Match: RS4_METHJ (30S ribosomal protein S4P OS=Methanospirillum hungatei (strain JF-1 / DSM 864) GN=rps4p PE=3 SV=1)

HSP 1 Score: 108.997 bits (271), Expect = 3.138e-23
Identity = 63/157 (40.13%), Postives = 93/157 (59.24%), Query Frame = 1
Query:  121 KTFKKPRRPYEKERLDAELRLVGEYGLRCKRELWRVQYALSRIRNAARDLLTLDE---KNPRRIFEGEALLRRMHRYGFLEEGQNKLDYVLALTVENFLERRLQTLVFKSGMAKSIHHARVLIRQRHIRVGRQLVNIPSFVVRVDSQKHIEFSLTSP 582
            K ++ P+RP+E  R++ E RLV EYGLR KRE+W  + AL + R AAR+++ L     +  R   + + L+  + R G L E  + +D VLAL VE  L+RRLQT V++ G A+S   AR  I   HI +G + V IP + V    Q+ I ++ +SP
Sbjct:    9 KQYQTPKRPFELSRIEEETRLVIEYGLRNKREVWIAKGALRKYRKAAREIIALQSTGSEQARVERKKDELIGSLQRSGMLSENAD-IDDVLALKVEQQLDRRLQTQVYRRGFARSPKQARQFITHGHIAIGGRRVTIPGYTVSAKEQEEISYAGSSP 164          
BLAST of CK933105 vs. ExPASy Swiss-Prot
Match: RS4_PYRKO (30S ribosomal protein S4P OS=Pyrococcus kodakaraensis GN=rps4p PE=3 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 9.129e-23
Identity = 66/164 (40.24%), Postives = 94/164 (57.32%), Query Frame = 1
Query:  121 KTFKKPRRPYEKERLDAELRLVGEYGLRCKRELWRVQYALSRIRNAARDLLTLDEKNPRRIFEGEALLRRMHRYGFLEEGQNKLDYVLALTVENFLERRLQTLVFKSGMAKSIHHARVLIRQRHIRVGRQLVNIPSFVVRVDSQKHIEFSLTSPFG-GGRPGRV 609
            K ++ P  P+ KERLD E  L   Y L+ K+ELWR +  L   R  AR LL    K      E + LL+R++R G L      LD VL+LTVE+ LERRLQT+V++ G+A+++  AR LI   HI V  Q++  P ++V  + +  I ++  SPF   G P R+
Sbjct:    9 KKYETPSHPWIKERLDRERVLKRNYALKNKKELWRHETQLKEFRRRARRLLAARGKQAE--IERQQLLQRLYRLGLLP-ADAVLDDVLSLTVEDVLERRLQTIVYRKGLARTMKQARQLIVHGHIEVNGQVIRSPGYLVLREEEDTITYAKGSPFAKEGHPERM 169          
BLAST of CK933105 vs. ExPASy Swiss-Prot
Match: RS4_METBF (30S ribosomal protein S4P OS=Methanosarcina barkeri (strain Fusaro / DSM 804) GN=rps4p PE=3 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 2.034e-22
Identity = 58/160 (36.25%), Postives = 92/160 (57.50%), Query Frame = 1
Query:  121 KTFKKPRRPYEKERLDAELRLVGEYGLRCKRELWRVQYALSRIRNAARDLLTLDEKNPRRIFEG------EALLRRMHRYGFLEEGQNKLDYVLALTVENFLERRLQTLVFKSGMAKSIHHARVLIRQRHIRVGRQLVNIPSFVVRVDSQKHIEFSLTSP 582
            K+F+ PR P+++ R+ +E++LV  YGLR KRE+W+    L   R+ AR LL     +  R  EG      E +L ++ RYG ++   + +D +L+L  EN LERRLQT V + G+A+++  AR  I   HI +  +   +P  +V  + + HI +  TSP
Sbjct:    9 KSFETPRHPWQEARMASEVQLVKAYGLRNKREVWKAASTLRMYRSEARRLLASAANSQERGLEGHQKTQSEEILAKLIRYGIIKSDAD-IDDILSLKTENILERRLQTQVLRLGLARTVVQARQFITHGHIAINGRKATVPGMLVSKEDEMHIGYYATSP 167          
BLAST of CK933105 vs. ExPASy Swiss-Prot
Match: RS4_PYRAE (30S ribosomal protein S4P OS=Pyrobaculum aerophilum GN=rps4p PE=3 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 2.656e-22
Identity = 59/137 (43.07%), Postives = 86/137 (62.77%), Query Frame = 1
Query:  133 KPRRPYEKERLDAELRLVGEYGLRCKRELWRVQYALSRIRNAARDLL--TLDEKNPRRIFEGEALLRRMHRYGFLEEGQNKLDYVLALTVENFLERRLQTLVFKSGMAKSIHHARVLIRQRHIRVGRQLVNIPSFVV 537
            KP++ + K+ L  EL+L+GEYGLR K+ELW  +  L  I   AR LL  T +E+ P  +        ++++ GF+E+   +LD +L+L V   LERRLQT+V++ G+AKSIHHAR LI   H+ V  + V  P F+V
Sbjct:   15 KPKKIWNKQLLLEELQLMGEYGLRNKKELWLARARLKWITRRARSLLSMTAEERAPLEM----PFKEKLYKMGFIEDPNVQLDRILSLDVRAILERRLQTIVYRMGLAKSIHHARQLIVHGHVAVAGRRVTSPGFLV 147          
BLAST of CK933105 vs. ExPASy Swiss-Prot
Match: RS4_PYRCJ (30S ribosomal protein S4P OS=Pyrobaculum calidifontis (strain JCM 11548 / VA1) GN=rps4p PE=3 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 3.469e-22
Identity = 60/137 (43.80%), Postives = 85/137 (62.04%), Query Frame = 1
Query:  133 KPRRPYEKERLDAELRLVGEYGLRCKRELWRVQYALSRIRNAARDLL--TLDEKNPRRIFEGEALLRRMHRYGFLEEGQNKLDYVLALTVENFLERRLQTLVFKSGMAKSIHHARVLIRQRHIRVGRQLVNIPSFVV 537
            KP++ + K+ L  EL+L+GEYGLR K+ELW  +  L  I   AR LL  T +E+ P  +        ++++ GF+E+    LD +L+L V   LERRLQTLVF+ G+AKSIHHAR L+   H+ V  + V  P F+V
Sbjct:   15 KPKKIWNKQLLLEELQLMGEYGLRNKKELWLARARLKWIVRRARALLSMTAEERAPLEV----PFKEKLYKMGFIEDPNVPLDRILSLDVRAILERRLQTLVFRMGLAKSIHHARQLVVHGHVLVAGRRVTSPGFLV 147          
BLAST of CK933105 vs. ExPASy Swiss-Prot
Match: RS4_CALMQ (30S ribosomal protein S4P OS=Caldivirga maquilingensis (strain DSMZ 13496 / IC-167) GN=rps4p PE=3 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 5.917e-22
Identity = 67/152 (44.08%), Postives = 91/152 (59.87%), Query Frame = 1
Query:  133 KPRRPYEKERLDAELRLVGEYGLRCKRELWRVQYALSRIRNAARDLLTLDEKNPRRIFEGEALLR---RMHRYGFLEEGQNKLDYVLALTVENFLERRLQTLVFKSGMAKSIHHARVLIRQRHIRVGRQLVNIPSFVVRVDSQKHIEFSLTS 579
            KP+R +  + L +ELRL+GEYGLR K+ELW  +  L  I++ AR++L+     P  I  GEA  R   R+ R G + +    LD VLAL V   LERRLQTLV++ GMA+SIH AR LI   HI +    V  P ++V  + +  I F+ TS
Sbjct:   15 KPKRLWNSDLLMSELRLIGEYGLRNKKELWLARALLRSIKHRAREILSA----PMEI-RGEAEKRLKDRLFRMGLISDINIPLDSVLALDVTAVLERRLQTLVYRKGMARSIHEARQLIVHGHISINGVRVRSPGYLVPRNLEDGIGFAPTS 161          
BLAST of CK933105 vs. ExPASy Swiss-Prot
Match: RS4_PYRIL (30S ribosomal protein S4P OS=Pyrobaculum islandicum (strain DSM 4184 / JCM 9189) GN=rps4p PE=3 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 1.009e-21
Identity = 59/145 (40.69%), Postives = 89/145 (61.38%), Query Frame = 1
Query:  133 KPRRPYEKERLDAELRLVGEYGLRCKRELWRVQYALSRIRNAARDLLTL--DEKNPRRIFEGEALLRRMHRYGFLEEGQNKLDYVLALTVENFLERRLQTLVFKSGMAKSIHHARVLIRQRHIRVGRQLVNIPSFVVRVDSQKHI 561
            KP++ + K+ L  EL+LVGEYGLR K+ELW  +  L  I   AR LL++  +E+ P  +        ++++ GF+E+    LD +L+L V   LERRLQT+V++ G+AKSI+HAR L+   H+ V  + V  P F+V  D +  I
Sbjct:   15 KPKKIWNKQLLLEELQLVGEYGLRNKKELWLARAHLKWIVRRARSLLSMTPEERAPLEL----PFKEKLYKMGFIEDPNVPLDRILSLDVRAILERRLQTIVYRKGLAKSIYHARQLVVHGHVAVAGRRVTSPGFLVPRDLEDKI 155          
The following BLAST results are available for this feature:
BLAST of CK933105 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 84
Match NameE-valueIdentityDescription
RS4_SULIA2.402e-2337.5030S ribosomal protein S4P OS=Sulfolobus islandicus... [more]
RS4_KORCO2.402e-2338.1630S ribosomal protein S4P OS=Korarchaeum cryptofil... [more]
RS4_THEVO3.138e-2340.7630S ribosomal protein S4P OS=Thermoplasma volcaniu... [more]
RS4_METHJ3.138e-2340.1330S ribosomal protein S4P OS=Methanospirillum hung... [more]
RS4_PYRKO9.129e-2340.2430S ribosomal protein S4P OS=Pyrococcus kodakaraen... [more]
RS4_METBF2.034e-2236.2530S ribosomal protein S4P OS=Methanosarcina barker... [more]
RS4_PYRAE2.656e-2243.0730S ribosomal protein S4P OS=Pyrobaculum aerophilu... [more]
RS4_PYRCJ3.469e-2243.8030S ribosomal protein S4P OS=Pyrobaculum calidifon... [more]
RS4_CALMQ5.917e-2244.0830S ribosomal protein S4P OS=Caldivirga maquilinge... [more]
RS4_PYRIL1.009e-2140.6930S ribosomal protein S4P OS=Pyrobaculum islandicu... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004347_F05 Developing fruit juice sac at 38 DAFB Citrus sinensis cDNA clone JSJune0004_IF_F05 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK933105 ID=CK933105; Name=CK933105; organism=Citrus sinensis; type=EST; length=797bp
GGGGCTCTTCACGCCCTTTGCTTAGGCTTTTACAAAGATCGCAGAGAGAG
AGAGAGAGAGTGAGGGTAGCACCAGCGGCGCGCAAAGATGGTGCACGTTT
CATTCTACCGCAACTATGGGAAAACCTTCAAGAAGCCAAGACGTCCCTAT
GAGAAGGAGCGATTGGATGCTGAGTTGAGGCTTGTGGGAGAGTATGGGCT
TCGCTGCAAGAGGGAACTCTGGAGGGTTCAATATGCATTGAGCCGCATCC
GAAATGCTGCGAGGGATCTTCTCACTCTCGATGAGAAGAACCCACGTCGT
ATTTTCGAGGGTGAGGCCCTTCTCAGGAGGATGCACAGGTACGGCTTCTT
GGAAGAGGGCCAGAACAAGCTTGATTATGTGTTGGCCCTCACTGTGGAGA
ACTTTCTTGAGCGTCGTCTCCAGACCCTTGTGTTCAAGAGTGGCATGGCT
AAGTCAATTCACCATGCTCGAGTGCTCATCAGGCAGAGGCACATTAGGGT
TGGAAGGCAGCTTGTCAACATTCCCTCATTTGTAGTGAGGGTTGATTCCC
AGAAGCACATTGAATTCTCACTCACAAGTCCATTCGGAGGTGGGCGCCCT
GGAAGAGTGAAGAGAAGGAACATCAAGGCTGCTGCCAAGAAGGCCGCAGG
TGGAGATGGGGATGAAGAGGATGAAGAATAAATTACCTGTGTGAACAGAT
AGTTTTTTTATGGTTACAGTAGGAATTCTGGCTGAACTTTGCAAGTGGAC
TTATCTTCTCTAAGTTTTATGTTTTTATTTGCGAATTTTCTTGCTAT
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