CK935599 | Citrus Genome Database

Overview


Name	CK935599
Unique Name	CK935599
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	858

Libraries


Library Name	Type
Developing fruit 24 DAFB	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of CK935599 vs. ExPASy Swiss-Prot
Match: MAO1_SALSV (NAD-dependent malic enzyme OS=Salmonella schwarzengrund (strain CVM19633) GN=sfcA PE=3 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 1.342e-48
Identity = 57/91 (62.64%), Postives = 68/91 (74.73%), Query Frame = 1

Query:  583 NWPERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFM 855
            N P  +I+VIVVTDGERILGLGD G  GMGIP+GKL+LYTA GGI P+  LP+ +DVGTNN+QLL D  Y+G R  R T  EY   +DEF+
Sbjct:  141 NVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRHPRITDDEYYAFVDEFI 231

HSP 2 Score: 94.7449 bits (234), Expect = 1.342e-48
Identity = 44/127 (34.65%), Postives = 81/127 (63.78%), Query Frame = 3

Query:  207 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVFEEL 587
            +G  LL  P  NKG AF+ +ER    L GLLP  V S + Q ++     + ++  + K++ +  +++ NE LFY+L+ +++EE++PV+YTPTVG AC+++  I+RR +G++IS   +  + ++ + +
Sbjct:   16 AGPVLLEFPLLNKGSAFSVEERRNFNLSGLLPEVVESIEEQAERAWLQYQGFKTEIDKHIYLRNIQDTNETLFYRLVQNHLEEMMPVIYTPTVGAACERFSEIYRRARGVFISYPNRHNMDDILQNV 142

BLAST of CK935599 vs. ExPASy Swiss-Prot
Match: MAO1_SALPB (NAD-dependent malic enzyme OS=Salmonella paratyphi B (strain ATCC BAA-1250 / SPB7) GN=sfcA PE=3 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 1.342e-48
Identity = 57/91 (62.64%), Postives = 68/91 (74.73%), Query Frame = 1

Query:  583 NWPERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFM 855
            N P  +I+VIVVTDGERILGLGD G  GMGIP+GKL+LYTA GGI P+  LP+ +DVGTNN+QLL D  Y+G R  R T  EY   +DEF+
Sbjct:  141 NVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRHPRITDDEYYAFVDEFI 231

HSP 2 Score: 94.7449 bits (234), Expect = 1.342e-48
Identity = 44/127 (34.65%), Postives = 81/127 (63.78%), Query Frame = 3

Query:  207 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVFEEL 587
            +G  LL  P  NKG AF+ +ER    L GLLP  V S + Q ++     + ++  + K++ +  +++ NE LFY+L+ +++EE++PV+YTPTVG AC+++  I+RR +G++IS   +  + ++ + +
Sbjct:   16 AGPVLLEFPLLNKGSAFSVEERRNFNLSGLLPEVVESIEEQAERAWLQYQGFKTEIDKHIYLRNIQDTNETLFYRLVQNHLEEMMPVIYTPTVGAACERFSEIYRRARGVFISYPNRHNMDDILQNV 142

BLAST of CK935599 vs. ExPASy Swiss-Prot
Match: MAO1_SALPA (NAD-dependent malic enzyme OS=Salmonella paratyphi A GN=sfcA PE=3 SV=2)

HSP 1 Score: 118.242 bits (295), Expect = 1.342e-48
Identity = 57/91 (62.64%), Postives = 68/91 (74.73%), Query Frame = 1

Query:  583 NWPERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFM 855
            N P  +I+VIVVTDGERILGLGD G  GMGIP+GKL+LYTA GGI P+  LP+ +DVGTNN+QLL D  Y+G R  R T  EY   +DEF+
Sbjct:  141 NVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRHPRITDDEYYAFVDEFI 231

HSP 2 Score: 94.7449 bits (234), Expect = 1.342e-48
Identity = 44/127 (34.65%), Postives = 81/127 (63.78%), Query Frame = 3

Query:  207 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVFEEL 587
            +G  LL  P  NKG AF+ +ER    L GLLP  V S + Q ++     + ++  + K++ +  +++ NE LFY+L+ +++EE++PV+YTPTVG AC+++  I+RR +G++IS   +  + ++ + +
Sbjct:   16 AGPVLLEFPLLNKGSAFSVEERRNFNLSGLLPEVVESIEEQAERAWLQYQGFKTEIDKHIYLRNIQDTNETLFYRLVQNHLEEMMPVIYTPTVGAACERFSEIYRRARGVFISYPNRHNMDDILQNV 142

BLAST of CK935599 vs. ExPASy Swiss-Prot
Match: MAO1_SALNS (NAD-dependent malic enzyme OS=Salmonella newport (strain SL254) GN=sfcA PE=3 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 1.342e-48
Identity = 57/91 (62.64%), Postives = 68/91 (74.73%), Query Frame = 1

Query:  583 NWPERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFM 855
            N P  +I+VIVVTDGERILGLGD G  GMGIP+GKL+LYTA GGI P+  LP+ +DVGTNN+QLL D  Y+G R  R T  EY   +DEF+
Sbjct:  141 NVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRHPRITDDEYYAFVDEFI 231

HSP 2 Score: 94.7449 bits (234), Expect = 1.342e-48
Identity = 44/127 (34.65%), Postives = 81/127 (63.78%), Query Frame = 3

Query:  207 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVFEEL 587
            +G  LL  P  NKG AF+ +ER    L GLLP  V S + Q ++     + ++  + K++ +  +++ NE LFY+L+ +++EE++PV+YTPTVG AC+++  I+RR +G++IS   +  + ++ + +
Sbjct:   16 AGPVLLEFPLLNKGSAFSVEERRNFNLSGLLPEVVESIEEQAERAWLQYQGFKTEIDKHIYLRNIQDTNETLFYRLVQNHLEEMMPVIYTPTVGAACERFSEIYRRARGVFISYPNRHNMDDILQNV 142

BLAST of CK935599 vs. ExPASy Swiss-Prot
Match: MAO1_SALHS (NAD-dependent malic enzyme OS=Salmonella heidelberg (strain SL476) GN=sfcA PE=3 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 1.342e-48
Identity = 57/91 (62.64%), Postives = 68/91 (74.73%), Query Frame = 1

Query:  583 NWPERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFM 855
            N P  +I+VIVVTDGERILGLGD G  GMGIP+GKL+LYTA GGI P+  LP+ +DVGTNN+QLL D  Y+G R  R T  EY   +DEF+
Sbjct:  141 NVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRHPRITDDEYYAFVDEFI 231

HSP 2 Score: 94.7449 bits (234), Expect = 1.342e-48
Identity = 44/127 (34.65%), Postives = 81/127 (63.78%), Query Frame = 3

Query:  207 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVFEEL 587
            +G  LL  P  NKG AF+ +ER    L GLLP  V S + Q ++     + ++  + K++ +  +++ NE LFY+L+ +++EE++PV+YTPTVG AC+++  I+RR +G++IS   +  + ++ + +
Sbjct:   16 AGPVLLEFPLLNKGSAFSVEERRNFNLSGLLPEVVESIEEQAERAWLQYQGFKTEIDKHIYLRNIQDTNETLFYRLVQNHLEEMMPVIYTPTVGAACERFSEIYRRARGVFISYPNRHNMDDILQNV 142

BLAST of CK935599 vs. ExPASy Swiss-Prot
Match: MAO1_SALEP (NAD-dependent malic enzyme OS=Salmonella enteritidis PT4 (strain P125109) GN=sfcA PE=3 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 1.342e-48
Identity = 57/91 (62.64%), Postives = 68/91 (74.73%), Query Frame = 1

Query:  583 NWPERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFM 855
            N P  +I+VIVVTDGERILGLGD G  GMGIP+GKL+LYTA GGI P+  LP+ +DVGTNN+QLL D  Y+G R  R T  EY   +DEF+
Sbjct:  141 NVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRHPRITDDEYYAFVDEFI 231

HSP 2 Score: 94.7449 bits (234), Expect = 1.342e-48
Identity = 44/127 (34.65%), Postives = 81/127 (63.78%), Query Frame = 3

Query:  207 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVFEEL 587
            +G  LL  P  NKG AF+ +ER    L GLLP  V S + Q ++     + ++  + K++ +  +++ NE LFY+L+ +++EE++PV+YTPTVG AC+++  I+RR +G++IS   +  + ++ + +
Sbjct:   16 AGPVLLEFPLLNKGSAFSVEERRNFNLSGLLPEVVESIEEQAERAWLQYQGFKTEIDKHIYLRNIQDTNETLFYRLVQNHLEEMMPVIYTPTVGAACERFSEIYRRARGVFISYPNRHNMDDILQNV 142

BLAST of CK935599 vs. ExPASy Swiss-Prot
Match: MAO1_SALDC (NAD-dependent malic enzyme OS=Salmonella dublin (strain CT_02021853) GN=sfcA PE=3 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 1.342e-48
Identity = 57/91 (62.64%), Postives = 68/91 (74.73%), Query Frame = 1

Query:  583 NWPERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFM 855
            N P  +I+VIVVTDGERILGLGD G  GMGIP+GKL+LYTA GGI P+  LP+ +DVGTNN+QLL D  Y+G R  R T  EY   +DEF+
Sbjct:  141 NVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRHPRITDDEYYAFVDEFI 231

HSP 2 Score: 94.7449 bits (234), Expect = 1.342e-48
Identity = 44/127 (34.65%), Postives = 81/127 (63.78%), Query Frame = 3

Query:  207 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVFEEL 587
            +G  LL  P  NKG AF+ +ER    L GLLP  V S + Q ++     + ++  + K++ +  +++ NE LFY+L+ +++EE++PV+YTPTVG AC+++  I+RR +G++IS   +  + ++ + +
Sbjct:   16 AGPVLLEFPLLNKGSAFSVEERRNFNLSGLLPEVVESIEEQAERAWLQYQGFKTEIDKHIYLRNIQDTNETLFYRLVQNHLEEMMPVIYTPTVGAACERFSEIYRRARGVFISYPNRHNMDDILQNV 142

BLAST of CK935599 vs. ExPASy Swiss-Prot
Match: MAO1_SALCH (NAD-dependent malic enzyme OS=Salmonella choleraesuis GN=sfcA PE=3 SV=2)

HSP 1 Score: 118.242 bits (295), Expect = 1.342e-48
Identity = 57/91 (62.64%), Postives = 68/91 (74.73%), Query Frame = 1

Query:  583 NWPERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFM 855
            N P  +I+VIVVTDGERILGLGD G  GMGIP+GKL+LYTA GGI P+  LP+ +DVGTNN+QLL D  Y+G R  R T  EY   +DEF+
Sbjct:  141 NVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRHPRITDDEYYAFVDEFI 231

HSP 2 Score: 94.7449 bits (234), Expect = 1.342e-48
Identity = 44/127 (34.65%), Postives = 81/127 (63.78%), Query Frame = 3

Query:  207 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVFEEL 587
            +G  LL  P  NKG AF+ +ER    L GLLP  V S + Q ++     + ++  + K++ +  +++ NE LFY+L+ +++EE++PV+YTPTVG AC+++  I+RR +G++IS   +  + ++ + +
Sbjct:   16 AGPVLLEFPLLNKGSAFSVEERRNFNLSGLLPEVVESIEEQAERAWLQYQGFKTEIDKHIYLRNIQDTNETLFYRLVQNHLEEMMPVIYTPTVGAACERFSEIYRRARGVFISYPNRHNMDDILQNV 142

BLAST of CK935599 vs. ExPASy Swiss-Prot
Match: MAO1_SALAR (NAD-dependent malic enzyme OS=Salmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980) GN=sfcA PE=3 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 1.342e-48
Identity = 57/91 (62.64%), Postives = 68/91 (74.73%), Query Frame = 1

Query:  583 NWPERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFM 855
            N P  +I+VIVVTDGERILGLGD G  GMGIP+GKL+LYTA GGI P+  LP+ +DVGTNN+QLL D  Y+G R  R T  EY   +DEF+
Sbjct:  141 NVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRHPRITDDEYYAFVDEFI 231

HSP 2 Score: 94.7449 bits (234), Expect = 1.342e-48
Identity = 44/127 (34.65%), Postives = 81/127 (63.78%), Query Frame = 3

Query:  207 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVFEEL 587
            +G  LL  P  NKG AF+ +ER    L GLLP  V S + Q ++     + ++  + K++ +  +++ NE LFY+L+ +++EE++PV+YTPTVG AC+++  I+RR +G++IS   +  + ++ + +
Sbjct:   16 AGPVLLEFPLLNKGSAFSVEERRNFNLSGLLPEVVESIEEQAERAWLQYQGFKTEIDKHIYLRNIQDTNETLFYRLVQNHLEEMMPVIYTPTVGAACERFSEIYRRARGVFISYPNRHNMDDILQNV 142

BLAST of CK935599 vs. ExPASy Swiss-Prot
Match: MAO1_SALA4 (NAD-dependent malic enzyme OS=Salmonella agona (strain SL483) GN=sfcA PE=3 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 1.342e-48
Identity = 57/91 (62.64%), Postives = 68/91 (74.73%), Query Frame = 1

Query:  583 NWPERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFM 855
            N P  +I+VIVVTDGERILGLGD G  GMGIP+GKL+LYTA GGI P+  LP+ +DVGTNN+QLL D  Y+G R  R T  EY   +DEF+
Sbjct:  141 NVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRHPRITDNEYYAFVDEFI 231

HSP 2 Score: 94.7449 bits (234), Expect = 1.342e-48
Identity = 44/127 (34.65%), Postives = 81/127 (63.78%), Query Frame = 3

Query:  207 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVFEEL 587
            +G  LL  P  NKG AF+ +ER    L GLLP  V S + Q ++     + ++  + K++ +  +++ NE LFY+L+ +++EE++PV+YTPTVG AC+++  I+RR +G++IS   +  + ++ + +
Sbjct:   16 AGPVLLEFPLLNKGSAFSVEERRNFNLSGLLPEVVESIEEQAERAWLQYQGFKTEIDKHIYLRNIQDTNETLFYRLVQNHLEEMMPVIYTPTVGAACERFSEIYRRARGVFISYPNRHNMDDILQNV 142

The following BLAST results are available for this feature:

BLAST of CK935599 vs. ExPASy Swiss-Prot

BLAST of CK935599 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 140


Match Name	E-value	Identity	Description
MAO1_SALSV	1.342e-48	62.64	NAD-dependent malic enzyme OS=Salmonella schwarzen...	[more]
MAO1_SALPB	1.342e-48	62.64	NAD-dependent malic enzyme OS=Salmonella paratyphi...	[more]
MAO1_SALPA	1.342e-48	62.64	NAD-dependent malic enzyme OS=Salmonella paratyphi...	[more]
MAO1_SALNS	1.342e-48	62.64	NAD-dependent malic enzyme OS=Salmonella newport (...	[more]
MAO1_SALHS	1.342e-48	62.64	NAD-dependent malic enzyme OS=Salmonella heidelber...	[more]
MAO1_SALEP	1.342e-48	62.64	NAD-dependent malic enzyme OS=Salmonella enteritid...	[more]
MAO1_SALDC	1.342e-48	62.64	NAD-dependent malic enzyme OS=Salmonella dublin (s...	[more]
MAO1_SALCH	1.342e-48	62.64	NAD-dependent malic enzyme OS=Salmonella choleraes...	[more]
MAO1_SALAR	1.342e-48	62.64	NAD-dependent malic enzyme OS=Salmonella arizonae ...	[more]
MAO1_SALA4	1.342e-48	62.64	NAD-dependent malic enzyme OS=Salmonella agona (st...	[more]

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Properties


Property Name	Value
Genbank description	CGF1004553_G07 Developing fruit 24 DAFB Citrus sinensis cDNA clone T24DAB0004_IIF_G07 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK935599 ID=CK935599; Name=CK935599; organism=Citrus sinensis; type=EST; length=858bp
GGGGTGTTTGTTTGTTTTCGTTTTTGTGAAAAGCAGAAAAAGTAAAAAGA
GTGAGAATGGAGAGCACCATGAAGGAGATGAGAGACGGAGCTTCGGTTCT
CGACTTCGACTCGAAATCCACCGTCGAAGGCGGCGTTGAAGACGTCTACA
GTGAGGATCGTGCCACCGAGGACCAGCTCGTCACTCCCTGGACCATCTCT
GTTGCCAGCGGGTACTGTTTGTTGAGGGATCCACGCCATAACAAAGGGCT
TGCTTTCACTGAGAAAGAGAGGGATGCCCACTACTTGCGCGGCCTTTTGC
CTCCAGCTGTTATAAGTCAACAACTTCAGGAGAAGAAATTGATGAACAGC
ATCAGACAATATGAAGTTCCGCTGCAAAAATATGTGGCCATGATGGAACT
TGAGGAGAGAAATGAAAGACTGTTCTATAAGCTACTGATTGATAATGTTG
AGGAACTTCTCCCAGTCGTTTACACTCCAACTGTTGGTGAAGCTTGCCAG
AAATATGGAAGTATCTTCAGGCGTCCTCAGGGTCTATACATAAGTCTGAA
AGAGAAGGGAAAGATTCTTGAGGTTTTTGAAGAACTGGCCTGAAAGGAGT
ATTCAGGTTATTGTTGTCACAGATGGTGAGCGCATTTTGGGACTTGGGGA
TCTTGGCTGTCAGGGGATGGGAATTCCTGTTGGGAAATTGGCTTTGTACA
CAGCACTTGGAGGCATTCGTCCCTCAGCATGTTTGCCCATAACCGTTGAT
GTGGGTACAAACAATGAGCAATTGTTGAAGGATGAATTCTACATTGGTCT
ACGACAGAGGAGGGCAACCGGACAGGAATATGCTGAACTTCTAGATGAAT
TCATGAGT