CK935645

Overview
NameCK935645
Unique NameCK935645
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length677
Libraries
Library NameType
Developing fruit 24 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig1498 contig Csv1_Contig1498:56..735. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CK935645 vs. ExPASy Swiss-Prot
Match: NDK3_ARATH (Nucleoside diphosphate kinase III, chloroplastic/mitochondrial OS=Arabidopsis thaliana GN=NDPK3 PE=1 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 1.472e-43
Identity = 73/135 (54.07%), Postives = 99/135 (73.33%), Query Frame = 2 
Query:    2 GILSHVCCAISLNSQNKLVSLNLKEMEQTFIMIKPDGVQRGLVGEIIIRFEKKGFSLKGLKLMTVDRPFAEKHYEDLSSKPFFGSLIEYITSGPVVAMIWEGEGVVKTGRTIIGATNPAQSAPGTIRGDLAIVTG 406
            G+L+    A  +  Q  L +    EME+TFI IKPDGVQRGL+ EII RFE+KGF L G+K++   + FA+KHY DL  +PFF  L ++++SGPV+AM+WEG+GV++ GR +IGAT+P +S PGTIRGDLA+  G
Sbjct:   67 GLLALPAAAYMIQDQEVLAA----EMERTFIAIKPDGVQRGLISEIISRFERKGFKLVGIKVIVPSKDFAQKHYHDLKERPFFNGLCDFLSSGPVIAMVWEGDGVIRYGRKLIGATDPQKSEPGTIRGDLAVTVG 197

HSP 2 Score: 40.817 bits (94), Expect = 1.472e-43
Identity = 18/37 (48.65%), Postives = 26/37 (70.27%), Query Frame = 1 
Query:  406 NIIHGSDSVESAQKEIALWF-PDGPINWQSSLHPWIY 513
            NIIHGSD  E+A+ EI+LWF P   +++ S+   W+Y
Sbjct:  199 NIIHGSDGPETAKDEISLWFKPQELVSYTSNSEKWLY 235
[Close]
BLAST of CK935645 vs. ExPASy Swiss-Prot
Match: NDK2_SPIOL (Nucleoside diphosphate kinase 2, chloroplastic OS=Spinacia oleracea GN=NDPK2 PE=1 SV=1)

HSP 1 Score: 154.836 bits (390), Expect = 1.472e-43
Identity = 73/114 (64.04%), Postives = 92/114 (80.70%), Query Frame = 2 
Query:   65 NLKEMEQTFIMIKPDGVQRGLVGEIIIRFEKKGFSLKGLKLMTVDRPFAEKHYEDLSSKPFFGSLIEYITSGPVVAMIWEGEGVVKTGRTIIGATNPAQSAPGTIRGDLAIVTG 406
            +++++E+T+IMIKPDGVQRGLVGEII RFEKKGF L GLK+    +  AE+HY+DL +K F+  LI+YITSGPVV M WEG GVV + R +IGAT+P Q+ PGTIRGDLA+ TG
Sbjct:   81 SMEQVEETYIMIKPDGVQRGLVGEIISRFEKKGFKLIGLKMYPCPKELAEEHYKDLKAKSFYQKLIDYITSGPVVCMAWEGVGVVASSRKLIGATDPLQAEPGTIRGDLAVQTG 194

HSP 2 Score: 43.5134 bits (101), Expect = 1.472e-43
Identity = 17/38 (44.74%), Postives = 26/38 (68.42%), Query Frame = 1 
Query:  406 NIIHGSDSVESAQKEIALWFPDGPI-NWQSSLHPWIYE 516
            N++HGSDS ++ ++EI LWF +G I  W  +  PW+ E
Sbjct:  196 NVVHGSDSPDNGKREIGLWFKEGEICQWTPAQAPWLRE 233
[Close]
BLAST of CK935645 vs. ExPASy Swiss-Prot
Match: NDKA_DROME (Nucleoside diphosphate kinase OS=Drosophila melanogaster GN=awd PE=1 SV=3)

HSP 1 Score: 147.902 bits (372), Expect = 1.487e-43
Identity = 70/109 (64.22%), Postives = 82/109 (75.23%), Query Frame = 2 
Query:   80 EQTFIMIKPDGVQRGLVGEIIIRFEKKGFSLKGLKLMTVDRPFAEKHYEDLSSKPFFGSLIEYITSGPVVAMIWEGEGVVKTGRTIIGATNPAQSAPGTIRGDLAIVTG 406
            E+TFIM+KPDGVQRGLVG+II RFE+KGF L  LK     +   EKHY DLS++PFF  L+ Y+ SGPVV M+WEG  VVKTGR ++GATNPA S PGTIRGD  I  G
Sbjct:    6 ERTFIMVKPDGVQRGLVGKIIERFEQKGFKLVALKFTWASKELLEKHYADLSARPFFPGLVNYMNSGPVVPMVWEGLNVVKTGRQMLGATNPADSLPGTIRGDFCIQVG 114

HSP 2 Score: 50.447 bits (119), Expect = 1.487e-43
Identity = 23/38 (60.53%), Postives = 28/38 (73.68%), Query Frame = 1 
Query:  406 NIIHGSDSVESAQKEIALWFPDGP-INWQSSLHPWIYE 516
            NIIHGSD+VESA+KEIALWF +   + W  +   WIYE
Sbjct:  116 NIIHGSDAVESAEKEIALWFNEKELVTWTPAAKDWIYE 153
[Close]
BLAST of CK935645 vs. ExPASy Swiss-Prot
Match: NDKA_DROYA (Nucleoside diphosphate kinase (Fragment) OS=Drosophila yakuba GN=awd PE=2 SV=1)

HSP 1 Score: 147.902 bits (372), Expect = 1.488e-43
Identity = 70/109 (64.22%), Postives = 82/109 (75.23%), Query Frame = 2 
Query:   80 EQTFIMIKPDGVQRGLVGEIIIRFEKKGFSLKGLKLMTVDRPFAEKHYEDLSSKPFFGSLIEYITSGPVVAMIWEGEGVVKTGRTIIGATNPAQSAPGTIRGDLAIVTG 406
            E+TFIM+KPDGVQRGLVG+II RFE+KGF L  LK     +   EKHY DLS++PFF  L+ Y+ SGPVV M+WEG  VVKTGR ++GATNPA S PGTIRGD  I  G
Sbjct:    3 ERTFIMVKPDGVQRGLVGKIIERFEQKGFKLVALKFTWASKELLEKHYADLSARPFFPGLVNYMNSGPVVPMVWEGLNVVKTGRQMLGATNPADSLPGTIRGDFCIQVG 111

HSP 2 Score: 50.447 bits (119), Expect = 1.488e-43
Identity = 23/38 (60.53%), Postives = 28/38 (73.68%), Query Frame = 1 
Query:  406 NIIHGSDSVESAQKEIALWFPDGP-INWQSSLHPWIYE 516
            NIIHGSD+VESA+KEIALWF +   + W  +   WIYE
Sbjct:  113 NIIHGSDAVESAEKEIALWFNEKELVTWTPAAKDWIYE 150
[Close]
BLAST of CK935645 vs. ExPASy Swiss-Prot
Match: NDK_NEUCR (Nucleoside diphosphate kinase OS=Neurospora crassa GN=ndk-1 PE=2 SV=2)

HSP 1 Score: 151.754 bits (382), Expect = 1.937e-43
Identity = 71/113 (62.83%), Postives = 85/113 (75.22%), Query Frame = 2 
Query:   68 LKEMEQTFIMIKPDGVQRGLVGEIIIRFEKKGFSLKGLKLMTVDRPFAEKHYEDLSSKPFFGSLIEYITSGPVVAMIWEGEGVVKTGRTIIGATNPAQSAPGTIRGDLAIVTG 406
            +   EQTFI +KPDGVQRGLVG II RFE +GF L  +KL    +   EKHYEDL++KPFF  LI+Y+ SGP+ AM+WEG+  VKTGRTI+GATNP  SAPGTIRGD A+  G
Sbjct:    1 MSNQEQTFIAVKPDGVQRGLVGNIISRFENRGFKLVAMKLTQPGQAHLEKHYEDLNTKPFFAGLIKYMNSGPICAMVWEGKDAVKTGRTILGATNPLASAPGTIRGDFALDMG 113

HSP 2 Score: 46.2098 bits (108), Expect = 1.937e-43
Identity = 21/38 (55.26%), Postives = 26/38 (68.42%), Query Frame = 1 
Query:  406 NIIHGSDSVESAQKEIALWFPDGPIN-WQSSLHPWIYE 516
            N+ HGSDSVE+A+KEIALWF    +N W      WI+E
Sbjct:  115 NVCHGSDSVENAKKEIALWFKPEELNQWNHHSAAWIFE 152
[Close]
BLAST of CK935645 vs. ExPASy Swiss-Prot
Match: NDKB_RAT (Nucleoside diphosphate kinase B OS=Rattus norvegicus GN=Nme2 PE=1 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 1.937e-43
Identity = 67/113 (59.29%), Postives = 82/113 (72.57%), Query Frame = 2 
Query:   68 LKEMEQTFIMIKPDGVQRGLVGEIIIRFEKKGFSLKGLKLMTVDRPFAEKHYEDLSSKPFFGSLIEYITSGPVVAMIWEGEGVVKTGRTIIGATNPAQSAPGTIRGDLAIVTG 406
            +  +E+TFI IKPDGVQRGLVGEII RFE+KGF L  +K +       ++HY DL  +PFF  L++Y+ SGPVVAM+WEG  VVKTGR ++G TNPA S PGTIRGD  I  G
Sbjct:    1 MANLERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVAMKFLRASEEHLKQHYIDLKDRPFFPGLVKYMNSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVG 113

HSP 2 Score: 55.0694 bits (131), Expect = 1.937e-43
Identity = 25/38 (65.79%), Postives = 31/38 (81.58%), Query Frame = 1 
Query:  406 NIIHGSDSVESAQKEIALWF-PDGPINWQSSLHPWIYE 516
            NIIHGSDSVESA+KEI LWF P+  I+++S  H W+YE
Sbjct:  115 NIIHGSDSVESAEKEIGLWFKPEELIDYKSCAHDWVYE 152
[Close]
BLAST of CK935645 vs. ExPASy Swiss-Prot
Match: NDKA1_BOVIN (Nucleoside diphosphate kinase A 1 OS=Bos taurus GN=NME1-1 PE=1 SV=3)

HSP 1 Score: 142.895 bits (359), Expect = 1.937e-43
Identity = 67/109 (61.47%), Postives = 80/109 (73.39%), Query Frame = 2 
Query:   80 EQTFIMIKPDGVQRGLVGEIIIRFEKKGFSLKGLKLMTVDRPFAEKHYEDLSSKPFFGSLIEYITSGPVVAMIWEGEGVVKTGRTIIGATNPAQSAPGTIRGDLAIVTG 406
            E+TFI IKPDGVQRGL+GEII RFE+KGF L  +K M       ++HY DL  +PFF  L++Y+ SGPVVAM+WEG  VVKTGR ++G TNPA S PGTIRGD  I  G
Sbjct:    5 ERTFIAIKPDGVQRGLIGEIIKRFEQKGFRLVAMKFMRASEDLLKEHYIDLKDRPFFAGLVKYMHSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVG 113

HSP 2 Score: 55.0694 bits (131), Expect = 1.937e-43
Identity = 26/38 (68.42%), Postives = 31/38 (81.58%), Query Frame = 1 
Query:  406 NIIHGSDSVESAQKEIALWF-PDGPINWQSSLHPWIYE 516
            NIIHGSDSVESA+KEIALWF P+  +N++S    WIYE
Sbjct:  115 NIIHGSDSVESAEKEIALWFHPEELVNYKSCAQNWIYE 152
[Close]
BLAST of CK935645 vs. ExPASy Swiss-Prot
Match: NDKA_PONAB (Nucleoside diphosphate kinase A OS=Pongo abelii GN=NME1 PE=2 SV=1)

HSP 1 Score: 146.747 bits (369), Expect = 2.524e-43
Identity = 70/109 (64.22%), Postives = 81/109 (74.31%), Query Frame = 2 
Query:   80 EQTFIMIKPDGVQRGLVGEIIIRFEKKGFSLKGLKLMTVDRPFAEKHYEDLSSKPFFGSLIEYITSGPVVAMIWEGEGVVKTGRTIIGATNPAQSAPGTIRGDLAIVTG 406
            E+TFI IKPDGVQRGLVGEII RFE+KGF L GLK M       ++HY DL  +PFF  L++Y+ SGPVVAM+WEG  VVKTGR ++G TNPA S PGTIRGD  I  G
Sbjct:    5 ERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVGLKFMQASEDLLKEHYVDLKDRPFFAGLVKYMHSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVG 113

HSP 2 Score: 50.8322 bits (120), Expect = 2.524e-43
Identity = 24/38 (63.16%), Postives = 29/38 (76.32%), Query Frame = 1 
Query:  406 NIIHGSDSVESAQKEIALWF-PDGPINWQSSLHPWIYE 516
            NIIHGSDSVESA+KEI LWF P+  +++ S    WIYE
Sbjct:  115 NIIHGSDSVESAEKEIGLWFHPEELVDYTSCAQNWIYE 152
[Close]
BLAST of CK935645 vs. ExPASy Swiss-Prot
Match: NDKA_HUMAN (Nucleoside diphosphate kinase A OS=Homo sapiens GN=NME1 PE=1 SV=1)

HSP 1 Score: 146.747 bits (369), Expect = 2.524e-43
Identity = 70/109 (64.22%), Postives = 81/109 (74.31%), Query Frame = 2 
Query:   80 EQTFIMIKPDGVQRGLVGEIIIRFEKKGFSLKGLKLMTVDRPFAEKHYEDLSSKPFFGSLIEYITSGPVVAMIWEGEGVVKTGRTIIGATNPAQSAPGTIRGDLAIVTG 406
            E+TFI IKPDGVQRGLVGEII RFE+KGF L GLK M       ++HY DL  +PFF  L++Y+ SGPVVAM+WEG  VVKTGR ++G TNPA S PGTIRGD  I  G
Sbjct:    5 ERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVGLKFMQASEDLLKEHYVDLKDRPFFAGLVKYMHSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVG 113

HSP 2 Score: 50.8322 bits (120), Expect = 2.524e-43
Identity = 24/38 (63.16%), Postives = 29/38 (76.32%), Query Frame = 1 
Query:  406 NIIHGSDSVESAQKEIALWF-PDGPINWQSSLHPWIYE 516
            NIIHGSDSVESA+KEI LWF P+  +++ S    WIYE
Sbjct:  115 NIIHGSDSVESAEKEIGLWFHPEELVDYTSCAQNWIYE 152
[Close]
BLAST of CK935645 vs. ExPASy Swiss-Prot
Match: NDKB_MOUSE (Nucleoside diphosphate kinase B OS=Mus musculus GN=Nme2 PE=1 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 3.290e-43
Identity = 67/113 (59.29%), Postives = 82/113 (72.57%), Query Frame = 2 
Query:   68 LKEMEQTFIMIKPDGVQRGLVGEIIIRFEKKGFSLKGLKLMTVDRPFAEKHYEDLSSKPFFGSLIEYITSGPVVAMIWEGEGVVKTGRTIIGATNPAQSAPGTIRGDLAIVTG 406
            +  +E+TFI IKPDGVQRGLVGEII RFE+KGF L  +K +       ++HY DL  +PFF  L++Y+ SGPVVAM+WEG  VVKTGR ++G TNPA S PGTIRGD  I  G
Sbjct:    1 MANLERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVAMKFLRASEEHLKQHYIDLKDRPFFPGLVKYMNSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVG 113

HSP 2 Score: 54.299 bits (129), Expect = 3.290e-43
Identity = 25/38 (65.79%), Postives = 31/38 (81.58%), Query Frame = 1 
Query:  406 NIIHGSDSVESAQKEIALWF-PDGPINWQSSLHPWIYE 516
            NIIHGSDSVESA+KEI LWF P+  I+++S  H W+YE
Sbjct:  115 NIIHGSDSVESAEKEIHLWFKPEELIDYKSCAHDWVYE 152
The following BLAST results are available for this feature:
BLAST of CK935645 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
NDK3_ARATH1.472e-4354.07Nucleoside diphosphate kinase III, chloroplastic/m... [more]
NDK2_SPIOL1.472e-4364.04Nucleoside diphosphate kinase 2, chloroplastic OS=... [more]
NDKA_DROME1.487e-4364.22Nucleoside diphosphate kinase OS=Drosophila melano... [more]
NDKA_DROYA1.488e-4364.22Nucleoside diphosphate kinase (Fragment) OS=Drosop... [more]
NDK_NEUCR1.937e-4362.83Nucleoside diphosphate kinase OS=Neurospora crassa... [more]
NDKB_RAT1.937e-4359.29Nucleoside diphosphate kinase B OS=Rattus norvegic... [more]
NDKA1_BOVIN1.937e-4361.47Nucleoside diphosphate kinase A 1 OS=Bos taurus GN... [more]
NDKA_PONAB2.524e-4364.22Nucleoside diphosphate kinase A OS=Pongo abelii GN... [more]
NDKA_HUMAN2.524e-4364.22Nucleoside diphosphate kinase A OS=Homo sapiens GN... [more]
NDKB_MOUSE3.290e-4359.29Nucleoside diphosphate kinase B OS=Mus musculus GN... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004553_C05 Developing fruit 24 DAFB Citrus sinensis cDNA clone T24DAB0004_IIF_C05 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK935645 ID=CK935645; Name=CK935645; organism=Citrus sinensis; type=EST; length=677bp
GGGGATTCTCTCGCATGTGTGCTGTGCTATTTCACTCAATTCTCAAAACA
AGTTGGTTTCTCTGAATCTCAAAGAAATGGAACAAACTTTCATCATGATC
AAGCCCGATGGGGTCCAAAGAGGCCTTGTTGGTGAAATCATCATCAGATT
TGAGAAGAAGGGTTTCTCTTTGAAGGGTTTGAAGCTTATGACTGTGGACC
GCCCTTTTGCTGAGAAGCACTATGAGGATTTGTCATCGAAGCCTTTCTTT
GGTTCTCTGATTGAGTACATTACCTCTGGTCCTGTTGTTGCTATGATTTG
GGAGGGTGAAGGCGTTGTTAAGACTGGCCGCACTATTATTGGAGCCACCA
ACCCTGCGCAGTCTGCTCCTGGAACCATCCGTGGTGATTTGGCTATCGTG
ACGGGAATATTATCCATGGAAGTGACTCAGTTGAGAGTGCCCAGAAAGAA
ATCGCCTTGTGGTTCCCTGATGGCCCTATTAACTGGCAGAGCAGCCTTCA
CCCTTGGATCTATGAGTAAACTAATGTTTGGTTGAGGAGCCTAATTGTTC
CTGCAGTTAGACTTATCCCTATCCCTATCCCTATTTCCTTATTTATGGTT
ACTCCTTGTTGGTGAACTGTTATGAAGTATTGAGTTAAATTGAGACCGAA
AAAAAAAAAAAAAAAAAAAAAAAAAAA
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