EY736386 | Citrus Genome Database

Overview


Name	EY736386
Unique Name	EY736386
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	870

Libraries


Library Name	Type
Sweet orange fruit, development stadium (6 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY736386 vs. ExPASy Swiss-Prot
Match: G3P2_TRIKO (Glyceraldehyde-3-phosphate dehydrogenase 2 OS=Trichoderma koningii GN=gpd2 PE=1 SV=3)

HSP 1 Score: 162.54 bits (410), Expect = 6.799e-41
Identity = 78/137 (56.93%), Postives = 99/137 (72.26%), Query Frame = 1

Query:  358 KVGINGFGRIGRLXXXXXXXXXXXXXXXXNDPFIDAKYMAYMFKYDSTHGVFKGTIKVVDDSTLEINGKLIKVFSKRDPAEIPWGD*GVDYVVESSGVFTTIAKASAHMKGGAKKVVISAPSADAPMFVVGVNEKTY 768
            KVGINGFGRIGR+                NDPFI+  Y AYM KYDS+HG+FKG ++V D   L +NGK ++ +++R+PA+I W + G +YVVES+GVFTT  KA AH+ GGAKKV+ISAPSADAPM+V+GVNE  Y
Sbjct:    5 KVGINGFGRIGRIVFRNAVEHPDIEVVAVNDPFIETTYAAYMLKYDSSHGLFKGEVEV-DGKDLVVNGKKVRFYTERNPADIKWSETGAEYVVESTGVFTTTEKAKAHLVGGAKKVIISAPSADAPMYVMGVNESDY 140

HSP 2 Score: 25.409 bits (54), Expect = 6.799e-41
Identity = 10/24 (41.67%), Postives = 15/24 (62.50%), Query Frame = 3

Query:  762 DIQANMNIVSNGKCTTNGLLLLAR 833
            D   + +++SN  CTTN L  LA+
Sbjct:  139 DYDGSADVISNASCTTNCLAPLAK 162

HSP 3 Score: 21.9422 bits (45), Expect = 6.799e-41
Identity = 8/18 (44.44%), Postives = 13/18 (72.22%), Query Frame = 2

Query:  815 LAPPCKGVQEEFGILEGV 868
            LAP  K + + +GI+EG+
Sbjct:  157 LAPLAKVINDNYGIVEGL 174

[Close]

BLAST of EY736386 vs. ExPASy Swiss-Prot
Match: G3PC_MESCR (Glyceraldehyde-3-phosphate dehydrogenase, cytosolic OS=Mesembryanthemum crystallinum GN=GAPC PE=2 SV=1)

HSP 1 Score: 158.303 bits (399), Expect = 6.801e-41
Identity = 84/139 (60.43%), Postives = 92/139 (66.19%), Query Frame = 1

Query:  358 KVGINGFGRIGRLXXXXXXXXXXXXXXXXNDPFIDAKYMAYMFKYDSTHGVFKG-TIKVVDDSTLEINGKLIKVFSKRDPAEIPWGD*GVDYVVESSGVFTTIAKASAHMKGGAKKVVISAPSADAPMFVVGVNEKTYK 771
            KVGINGFGRIGRL                NDPFI   YM YMFKYDS HG  K   IK+ D+ TL      + VF  R+P EIPWG  G D+VVES+GVFT   KA+AH+KGGAKKVVISAPS DAPMFVVGVNE  YK
Sbjct:    5 KVGINGFGRIGRLVARVILQRDDCELVAVNDPFISTDYMTYMFKYDSVHGQCKSHEIKLKDEKTLLFGETPVAVFGCRNPEEIPWGQAGADFVVESTGVFTDKDKAAAHLKGGAKKVVISAPSKDAPMFVVGVNEHEYK 143

HSP 2 Score: 28.4906 bits (62), Expect = 6.801e-41
Identity = 12/24 (50.00%), Postives = 18/24 (75.00%), Query Frame = 3

Query:  762 DIQANMNIVSNGKCTTNGLLLLAR 833
            + ++++NIVSN  CTTN L  LA+
Sbjct:  141 EYKSDLNIVSNASCTTNCLAPLAK 164

HSP 3 Score: 23.0978 bits (48), Expect = 6.801e-41
Identity = 9/18 (50.00%), Postives = 13/18 (72.22%), Query Frame = 2

Query:  815 LAPPCKGVQEEFGILEGV 868
            LAP  K + + FGI+EG+
Sbjct:  159 LAPLAKVINDRFGIVEGL 176

[Close]

BLAST of EY736386 vs. ExPASy Swiss-Prot
Match: G3P2_CAEBR (Glyceraldehyde-3-phosphate dehydrogenase 2 OS=Caenorhabditis briggsae GN=gpd-2 PE=3 SV=1)

HSP 1 Score: 165.622 bits (418), Expect = 7.913e-41
Identity = 87/140 (62.14%), Postives = 99/140 (70.71%), Query Frame = 1

Query:  361 VGINGFGRIGRLXXXXXXXXXXXXXXXXNDPFIDAKYMAYMFKYDSTHGVFKGTIKVVDDSTLEIN-GKL---IKVFSKRDPAEIPWGD*GVDYVVESSGVFTTIAKASAHMKGGAKKVVISAPSADAPMFVVGVNEKTY 768
            VGINGFGRIGRL                NDPFI   YM Y+F+YDSTHG FKGT+K   D  +  N GK    IKV++ +DPAEI WG  G DYVVES+GVFTTI KA+AH+KGGAKKV+ISAPSADAPMFVVGVN + Y
Sbjct:    6 VGINGFGRIGRLVLRAAVEKDSVNVVAVNDPFISIDYMVYLFQYDSTHGRFKGTVKHEGDYLIVANEGKSQHKIKVYNSKDPAEIQWGAAGADYVVESTGVFTTIEKANAHLKGGAKKVIISAPSADAPMFVVGVNHEKY 145

HSP 2 Score: 23.483 bits (49), Expect = 7.913e-41
Identity = 9/18 (50.00%), Postives = 13/18 (72.22%), Query Frame = 2

Query:  815 LAPPCKGVQEEFGILEGV 868
            LAP  K + + FGI+EG+
Sbjct:  163 LAPLAKVINDNFGIIEGL 180

[Close]

BLAST of EY736386 vs. ExPASy Swiss-Prot
Match: G3P1_GLORO (Glyceraldehyde-3-phosphate dehydrogenase 1 (Fragment) OS=Globodera rostochiensis GN=GPD-1 PE=2 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 7.919e-41
Identity = 86/143 (60.14%), Postives = 98/143 (68.53%), Query Frame = 1

Query:  358 KVGINGFGRIGRLXXXXXXXXXXXXXXXXNDPFIDAKYMAYMFKYDSTHGVFKGTIKV-VDDSTLEINGKL---IKVFSKRDPAEIPWGD*GVDYVVESSGVFTTIAKASAHMKGGAKKVVISAPSADAPMFVVGVNEKTYKP 774
            KVGINGFGRIGRL                NDPFI+  YM YMF YDSTHG F G I     +  +E  GK    IKVF+ +DPAEI W + G +YV+ES+GVFTTI KASAH+KGGAKKVVISAPSADAPM+V+GVNE  Y P
Sbjct:    5 KVGINGFGRIGRLALRAAVEKDTVQVVAINDPFIELDYMVYMFNYDSTHGRFNGKISTSAGNLVVEKEGKATHTIKVFNLKDPAEIKWAEVGAEYVIESTGVFTTIEKASAHLKGGAKKVVISAPSADAPMYVMGVNEDKYDP 147

HSP 2 Score: 25.409 bits (54), Expect = 7.919e-41
Identity = 10/18 (55.56%), Postives = 14/18 (77.78%), Query Frame = 2

Query:  815 LAPPCKGVQEEFGILEGV 868
            LAP  K + +EFGI+EG+
Sbjct:  163 LAPLAKVINDEFGIIEGL 180

[Close]

BLAST of EY736386 vs. ExPASy Swiss-Prot
Match: G3P_CANAL (Glyceraldehyde-3-phosphate dehydrogenase OS=Candida albicans GN=TDH1 PE=1 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 8.846e-41
Identity = 83/139 (59.71%), Postives = 94/139 (67.63%), Query Frame = 1

Query:  358 KVGINGFGRIGRLXXXXXXXXXXXXXXXXNDPFIDAKYMAYMFKYDSTHGVFKGTIKVVDDSTLEINGKLIKVFSKRDPAEIPWGD*GVDYVVESSGVFTTIAKASAHMKGGAKKVVISAPSADAPMFVVGVNEKTYKP 774
            K+GINGFGRIGRL                NDPFI   Y AYMFKYDSTHG +KG +    D  L I+G  IKVF +RDPA IPWG  GVDYV+ES+GVFT +  A  H+  GAKKV+I+APSADAPMFVVGVNE  Y P
Sbjct:    4 KIGINGFGRIGRLVLRVALGRKDIEVVAVNDPFIAPDYAAYMFKYDSTHGRYKGEVTASGDD-LVIDGHKIKVFQERDPANIPWGKSGVDYVIESTGVFTKVEGAQKHIDAGAKKVIITAPSADAPMFVVGVNEDKYTP 141

HSP 2 Score: 24.6386 bits (52), Expect = 8.846e-41
Identity = 10/20 (50.00%), Postives = 14/20 (70.00%), Query Frame = 3

Query:  774 NMNIVSNGKCTTNGLLLLAR 833
            ++ I+SN  CTTN L  LA+
Sbjct:  142 DLKIISNASCTTNCLAPLAK 161

HSP 3 Score: 21.557 bits (44), Expect = 8.846e-41
Identity = 10/18 (55.56%), Postives = 12/18 (66.67%), Query Frame = 2

Query:  815 LAPPCKGVQEEFGILEGV 868
            LAP  K V + FGI EG+
Sbjct:  156 LAPLAKVVNDTFGIEEGL 173

[Close]

BLAST of EY736386 vs. ExPASy Swiss-Prot
Match: G3P_PHACH (Glyceraldehyde-3-phosphate dehydrogenase OS=Phanerochaete chrysosporium GN=GPD PE=3 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 1.150e-40
Identity = 81/137 (59.12%), Postives = 95/137 (69.34%), Query Frame = 1

Query:  358 KVGINGFGRIGRLXXXXXXXXXXXXXXXXNDPFIDAKYMAYMFKYDSTHGVFKGTIKVVDDSTLEINGKLIKVFSKRDPAEIPWGD*GVDYVVESSGVFTTIAKASAHMKGGAKKVVISAPSADAPMFVVGVNEKTY 768
            K GINGFGRIGR+                NDPFID +YM YMFKYDS HG FKG+++   D  L + GK I VF+++DPA IPWG  G +Y+VES+GVFTT  KASAH+KG  KKV+ISAPSADAPMFV GVN   Y
Sbjct:    4 KAGINGFGRIGRIVLRNALLHGDIDVVAVNDPFIDLEYMVYMFKYDSVHGRFKGSVEA-KDGKLYVEGKPIHVFAEKDPANIPWGSVGAEYIVESTGVFTTTEKASAHLKGVCKKVIISAPSADAPMFVCGVNLDAY 139

HSP 2 Score: 23.0978 bits (48), Expect = 1.150e-40
Identity = 9/17 (52.94%), Postives = 12/17 (70.59%), Query Frame = 3

Query:  783 IVSNGKCTTNGLLLLAR 833
            ++SN  CTTN L  LA+
Sbjct:  145 VISNASCTTNCLAPLAK 161

HSP 3 Score: 22.3274 bits (46), Expect = 1.150e-40
Identity = 8/18 (44.44%), Postives = 14/18 (77.78%), Query Frame = 2

Query:  815 LAPPCKGVQEEFGILEGV 868
            LAP  K + ++FGI++G+
Sbjct:  156 LAPLAKVIHDKFGIVQGL 173

[Close]

BLAST of EY736386 vs. ExPASy Swiss-Prot
Match: G3P_DICDI (Glyceraldehyde-3-phosphate dehydrogenase OS=Dictyostelium discoideum GN=gpdA PE=1 SV=2)

HSP 1 Score: 160.614 bits (405), Expect = 1.495e-40
Identity = 76/137 (55.47%), Postives = 98/137 (71.53%), Query Frame = 1

Query:  361 VGINGFGRIGRLXXXXXXXXXXXXXXXXNDPFIDAKYMAYMFKYDSTHGVFKGTIKVVDDSTLEINGKLIKVFSKRDPAEIPWGD*GVDYVVESSGVFTTIAKASAHMKGGAKKVVISAPSADAPMFVVGVNEKTYK 771
            +GINGFGRIGRL                N+PF+D KYM YMFKYDSTHG FKGT++ ++   + +NG  I VF+++DPA I W   G +Y+VES+G+F +  KA  H+KGGAKKVVISAPS DAPM+V+GVNE+TY+
Sbjct:    5 IGINGFGRIGRLVLRASLENPECRVLAINEPFMDVKYMVYMFKYDSTHGRFKGTVEDINGEFV-VNGNKIHVFAEKDPANIKWSSVGAEYIVESTGLFLSTEKAGVHLKGGAKKVVISAPSTDAPMYVMGVNEETYE 140

HSP 2 Score: 24.2534 bits (51), Expect = 1.495e-40
Identity = 9/22 (40.91%), Postives = 16/22 (72.73%), Query Frame = 3

Query:  768 QANMNIVSNGKCTTNGLLLLAR 833
            +++ +++SN  CTTN L  LA+
Sbjct:  140 ESSHDVISNASCTTNCLAPLAK 161

HSP 3 Score: 23.8682 bits (50), Expect = 1.495e-40
Identity = 10/18 (55.56%), Postives = 13/18 (72.22%), Query Frame = 2

Query:  815 LAPPCKGVQEEFGILEGV 868
            LAP  K + E FGI+EG+
Sbjct:  156 LAPLAKIIHENFGIVEGL 173

[Close]

BLAST of EY736386 vs. ExPASy Swiss-Prot
Match: G3PC_ANTMA (Glyceraldehyde-3-phosphate dehydrogenase, cytosolic OS=Antirrhinum majus GN=GAPC PE=2 SV=1)

HSP 1 Score: 157.147 bits (396), Expect = 1.495e-40
Identity = 81/139 (58.27%), Postives = 94/139 (67.63%), Query Frame = 1

Query:  358 KVGINGFGRIGRLXXXXXXXXXXXXXXXXNDPFIDAKYMAYMFKYDSTHGVFKG-TIKVVDDSTLEINGKLIKVFSKRDPAEIPWGD*GVDYVVESSGVFTTIAKASAHMKGGAKKVVISAPSADAPMFVVGVNEKTYK 771
            K+GINGFGRIGRL                NDPFI   YM YMFKYDS HG +K   +KV D+ TL    K + VF +R+P EIP    G +Y+VES+GVFT   KA+AH+KGGAKKV+ISAPS DAPMFVVGVNEK YK
Sbjct:    5 KIGINGFGRIGRLVARVALQRDDVELVAVNDPFISTDYMTYMFKYDSVHGAWKHHELKVKDEKTLLFGEKPVVVFGRRNPEEIPRASTGAEYIVESTGVFTDKDKAAAHLKGGAKKVIISAPSKDAPMFVVGVNEKEYK 143

HSP 2 Score: 28.4906 bits (62), Expect = 1.495e-40
Identity = 12/25 (48.00%), Postives = 19/25 (76.00%), Query Frame = 3

Query:  759 KDIQANMNIVSNGKCTTNGLLLLAR 833
            K+ +++++IVSN  CTTN L  LA+
Sbjct:  140 KEYKSDLHIVSNASCTTNCLAPLAK 164

HSP 3 Score: 23.0978 bits (48), Expect = 1.495e-40
Identity = 9/18 (50.00%), Postives = 13/18 (72.22%), Query Frame = 2

Query:  815 LAPPCKGVQEEFGILEGV 868
            LAP  K + + FGI+EG+
Sbjct:  159 LAPLAKVINDRFGIVEGL 176

[Close]

BLAST of EY736386 vs. ExPASy Swiss-Prot
Match: G3P_HOMAM (Glyceraldehyde-3-phosphate dehydrogenase OS=Homarus americanus PE=1 SV=2)

HSP 1 Score: 166.392 bits (420), Expect = 1.913e-40
Identity = 88/149 (59.06%), Postives = 103/149 (69.13%), Query Frame = 1

Query:  355 TKVGINGFGRIGRLXXXXXXXXXXXXXXXXNDPFIDAKYMAYMFKYDSTHGVFKGTIKVVDDSTLEINGKLIKVFSKRDPAEIPWGD*GVDYVVESSGVFTTIAKASAHMKGGAKKVVISAPSADAPMFVVGVNEKTYKPT*TLFLMAS 801
            +K+GINGFGRIGRL                NDPFI  +YM YMFKYDSTHGVFKG +K+ +D  L ++GK I VF++  P  IPW   G +Y+VES+GVFTTI KASAH KGGAKKVVISAPSADAPMFV GVN + Y    T+   AS
Sbjct:    1 SKIGINGFGRIGRLVLRAALSCGAQVVAV-NDPFIALEYMVYMFKYDSTHGVFKGEVKM-EDGALVVDGKKITVFNEMKPENIPWSKAGAEYIVESTGVFTTIEKASAHFKGGAKKVVISAPSADAPMFVCGVNLEKYSKDMTVVSNAS 147

[Close]

BLAST of EY736386 vs. ExPASy Swiss-Prot
Match: G3PD_MAIZE (Glyceraldehyde-3-phosphate dehydrogenase, cytosolic 2 OS=Zea mays GN=GAPC2 PE=2 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 1.943e-40
Identity = 82/141 (58.16%), Postives = 94/141 (66.67%), Query Frame = 1

Query:  349 GNTKVGINGFGRIGRLXXXXXXXXXXXXXXXXNDPFIDAKYMAYMFKYDSTHGVFKGT-IKVVDDSTLEINGKLIKVFSKRDPAEIPWGD*GVDYVVESSGVFTTIAKASAHMKGGAKKVVISAPSADAPMFVVGVNEKTY 768
            G  K+GINGFGRIGRL                NDPFI   YM YMFKYD+ HG +K + I + D  TL    K + VF  R+P EIPWG+ G +YVVES+GVFT   KA+AH+KGGAKKVVISAPS DAPMFVVGVNE  Y
Sbjct:    2 GKIKIGINGFGRIGRLVARVALQSEDVELVAVNDPFITTDYMTYMFKYDTVHGQWKHSDIALKDSKTLLFGEKPVTVFGIRNPEEIPWGEAGAEYVVESTGVFTDKDKAAAHLKGGAKKVVISAPSKDAPMFVVGVNEDKY 142

HSP 2 Score: 27.335 bits (59), Expect = 1.943e-40
Identity = 12/21 (57.14%), Postives = 16/21 (76.19%), Query Frame = 3

Query:  771 ANMNIVSNGKCTTNGLLLLAR 833
            +++NIVSN  CTTN L  LA+
Sbjct:  144 SDVNIVSNASCTTNCLAPLAK 164

HSP 3 Score: 23.483 bits (49), Expect = 1.943e-40
Identity = 9/18 (50.00%), Postives = 13/18 (72.22%), Query Frame = 2

Query:  815 LAPPCKGVQEEFGILEGV 868
            LAP  K + + FGI+EG+
Sbjct:  159 LAPLAKVIHDNFGIIEGL 176

The following BLAST results are available for this feature:

BLAST of EY736386 vs. ExPASy Swiss-Prot

BLAST of EY736386 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 336


Match Name	E-value	Identity	Description
G3P2_TRIKO	6.799e-41	56.93	Glyceraldehyde-3-phosphate dehydrogenase 2 OS=Tric...	[more]
G3PC_MESCR	6.801e-41	60.43	Glyceraldehyde-3-phosphate dehydrogenase, cytosoli...	[more]
G3P2_CAEBR	7.913e-41	62.14	Glyceraldehyde-3-phosphate dehydrogenase 2 OS=Caen...	[more]
G3P1_GLORO	7.919e-41	60.14	Glyceraldehyde-3-phosphate dehydrogenase 1 (Fragme...	[more]
G3P_CANAL	8.846e-41	59.71	Glyceraldehyde-3-phosphate dehydrogenase OS=Candid...	[more]
G3P_PHACH	1.150e-40	59.12	Glyceraldehyde-3-phosphate dehydrogenase OS=Phaner...	[more]
G3P_DICDI	1.495e-40	55.47	Glyceraldehyde-3-phosphate dehydrogenase OS=Dictyo...	[more]
G3PC_ANTMA	1.495e-40	58.27	Glyceraldehyde-3-phosphate dehydrogenase, cytosoli...	[more]
G3P_HOMAM	1.913e-40	59.06	Glyceraldehyde-3-phosphate dehydrogenase OS=Homaru...	[more]
G3PD_MAIZE	1.943e-40	58.16	Glyceraldehyde-3-phosphate dehydrogenase, cytosoli...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C3-705-015-F11-CT.F Sweet orange fruit, development stadium (6 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY736386 ID=EY736386; Name=EY736386; organism=Citrus sinensis; type=EST; length=870bp
CACGCGTCCGCGAAAGCCGCAACAACCCAAACCTCGTTAGGGTTTTTGCG
AGAAGAGAGCTCGAAACAGTGTGCTTAAGCTCAACAAATGGCCTTCTCTT
CTCTCCTCAGATCTACGGCCTCCGCTTCTCTCGTCCGCGCCGACCTCACT
TCCTCGCCGTCCGATCGCGTCAAGGGATCATCGACTGCTGCTTTCAGTCG
CAACCTTAACACTTCGAGCATTTTCGGTACTTCAGTTCCAAGTGGTTCAT
CTTCTTCATCACTACAGACATGTGCTGCAAAGAGTATTCAACCCATCAGA
GCCACAGCTACTGAAATTCCTCCAACAATTCAAAAATCACGGAGTGATGG
AAACACAAAGGTCGGAATCAATGGTTTTGGTCGGATTGGAAGGTTGGTTT
TGCGAGTAGCAGCCTTCAGGGATGATGTTGACGTTGTGGCGGTCAATGAT
CCTTTTATTGATGCCAAATACATGGCTTACATGTTTAAGTATGATTCTAC
TCATGGAGTTTTCAAGGGAACCATCAAGGTCGTGGATGACTCCACCTTAG
AAATTAATGGGAAGCTGATTAAAGTTTTCAGCAAAAGGGACCCGGCAGAG
ATCCCTTGGGGTGACTAGGGGGTTGATTATGTTGTTGAATCTTCTGGAGT
CTTTACAACAATTGCGAAGGCTTCAGCTCACATGAAGGGTGGTGCAAAAA
AGGTGGTAATATCAGCTCCATCAGCTGATGCACCAATGTTTGTGGTAGGA
GTGAATGAAAAGACATACAAGCCAACATGAACATTGTTTCTAATGGCAAG
TGTACAACCAATGGCTTGCTCCTCCTTGCAAGGGGGTTCAGGAGGAGTTT
GGAATTCTGGAAGGCGTAAG