EY736121

Overview
NameEY736121
Unique NameEY736121
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length972
Libraries
Library NameType
Sweet orange fruit, development stadium (6 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY736121 vs. ExPASy Swiss-Prot
Match: GPMI_PSEP1 (2,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=gpmI PE=3 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 5.326e-15
Identity = 30/72 (41.67%), Postives = 42/72 (58.33%), Query Frame = 3 
Query:   99 GEYVVHNGVRTFACSETVKFGHVTCFWNGNRSGYFDSNLEEYVEIPSDSGITFNVQPTMKALEIADRAKKAI 314
            GEY+  NG      +ET K+ HVT F++G R   F+   EE + IPS    T+++QP M A E+ DR  +AI
Sbjct:  315 GEYLAKNGKTQLRIAETEKYAHVTFFFSGGREEPFEG--EERILIPSPKVATYDLQPEMSAPEVTDRIVEAI 384

HSP 2 Score: 43.5134 bits (101), Expect = 5.326e-15
Identity = 25/52 (48.08%), Postives = 29/52 (55.77%), Query Frame = 1 
Query:  328 FHQVRVNLPNIDMVVHTGDIEADVVACNAADNTVKIIIDAIHKFGGVYLVEA 483
            F  + VN  N DMV HTG  EA V A  A D  V  I+DA+ K GG  L+ A
Sbjct:  389 FDVIVVNYANGDMVGHTGVFEAAVKAVEALDTCVGRIVDALEKVGGEALITA 440
[Close]
BLAST of EY736121 vs. ExPASy Swiss-Prot
Match: GPMI_PSEPK (2,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Pseudomonas putida (strain KT2440) GN=gpmI PE=3 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 6.920e-15
Identity = 30/72 (41.67%), Postives = 42/72 (58.33%), Query Frame = 3 
Query:   99 GEYVVHNGVRTFACSETVKFGHVTCFWNGNRSGYFDSNLEEYVEIPSDSGITFNVQPTMKALEIADRAKKAI 314
            GEY+  NG      +ET K+ HVT F++G R   F+   EE + IPS    T+++QP M A E+ DR  +AI
Sbjct:  315 GEYLAKNGKTQLRIAETEKYAHVTFFFSGGREEPFEG--EERILIPSPKVATYDLQPEMSAPEVTDRIVEAI 384

HSP 2 Score: 43.1282 bits (100), Expect = 6.920e-15
Identity = 25/52 (48.08%), Postives = 29/52 (55.77%), Query Frame = 1 
Query:  328 FHQVRVNLPNIDMVVHTGDIEADVVACNAADNTVKIIIDAIHKFGGVYLVEA 483
            F  + VN  N DMV HTG  EA V A  A D  V  I+DA+ K GG  L+ A
Sbjct:  389 FDVIVVNYANGDMVGHTGVFEAAVKAVEALDTCVGRIVDALDKVGGEALITA 440
[Close]
BLAST of EY736121 vs. ExPASy Swiss-Prot
Match: GPMI_PSEPG (2,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Pseudomonas putida (strain GB-1) GN=gpmI PE=3 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.168e-14
Identity = 29/72 (40.28%), Postives = 42/72 (58.33%), Query Frame = 3 
Query:   99 GEYVVHNGVRTFACSETVKFGHVTCFWNGNRSGYFDSNLEEYVEIPSDSGITFNVQPTMKALEIADRAKKAI 314
            GEY+  NG      +ET K+ HVT F++G R   F+   EE + IPS    T+++QP M A ++ DR  +AI
Sbjct:  315 GEYLAKNGKTQLRIAETEKYAHVTFFFSGGREEPFEG--EERILIPSPKVATYDLQPEMNAPQVTDRIVEAI 384

HSP 2 Score: 43.5134 bits (101), Expect = 1.168e-14
Identity = 25/52 (48.08%), Postives = 29/52 (55.77%), Query Frame = 1 
Query:  328 FHQVRVNLPNIDMVVHTGDIEADVVACNAADNTVKIIIDAIHKFGGVYLVEA 483
            F  + VN  N DMV HTG  EA V A  A D  V  I+DA+ K GG  L+ A
Sbjct:  389 FDVIVVNYANGDMVGHTGVFEAAVKAVEALDTCVGRIVDALEKVGGEALITA 440
[Close]
BLAST of EY736121 vs. ExPASy Swiss-Prot
Match: GPMI_PSEE4 (2,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Pseudomonas entomophila (strain L48) GN=gpmI PE=3 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 1.972e-14
Identity = 30/72 (41.67%), Postives = 42/72 (58.33%), Query Frame = 3 
Query:   99 GEYVVHNGVRTFACSETVKFGHVTCFWNGNRSGYFDSNLEEYVEIPSDSGITFNVQPTMKALEIADRAKKAI 314
            GEY+  NG      +ET K+ HVT F++G R   F+   EE + IPS    T+++QP M A E+ DR  +AI
Sbjct:  315 GEYLAKNGKTQLRIAETEKYAHVTFFFSGGREEPFEG--EERILIPSPKVATYDLQPEMNAPEVTDRIVEAI 384

HSP 2 Score: 41.5874 bits (96), Expect = 1.972e-14
Identity = 24/52 (46.15%), Postives = 30/52 (57.69%), Query Frame = 1 
Query:  328 FHQVRVNLPNIDMVVHTGDIEADVVACNAADNTVKIIIDAIHKFGGVYLVEA 483
            F  + VN  N DMV HTG  EA V A  A D+ V  I++A+ K GG  L+ A
Sbjct:  389 FDVIVVNYANGDMVGHTGVFEAAVKAVEALDSCVGRIVEALGKVGGEALITA 440
[Close]
BLAST of EY736121 vs. ExPASy Swiss-Prot
Match: GPMI_GLUOX (2,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Gluconobacter oxydans GN=gpmI PE=3 SV=1)

HSP 1 Score: 51.2174 bits (121), Expect = 5.619e-14
Identity = 29/72 (40.28%), Postives = 39/72 (54.17%), Query Frame = 3 
Query:   99 GEYVVHNGVRTFACSETVKFGHVTCFWNGNRSGYFDSNLEEYVEIPSDSGITFNVQPTMKALEIADRAKKAI 314
            G+ V   G      +ET K+ HVT F NG R   FD   EE + +PS    T+++QP M A E+ D+A  AI
Sbjct:  315 GDVVAKAGRTQLRMAETEKYPHVTYFLNGGREVQFDG--EERILVPSPKVATYDLQPEMSAPELTDKAVAAI 384

HSP 2 Score: 45.4394 bits (106), Expect = 5.619e-14
Identity = 25/57 (43.86%), Postives = 30/57 (52.63%), Query Frame = 1 
Query:  328 FHQVRVNLPNIDMVVHTGDIEADVVACNAADNTVKIIIDAIHKFGGVYLVEAGSWLC 498
            F  + +N  N DMV HTG + A + AC   D  +  I DAI K GGV LV A    C
Sbjct:  389 FDMIVLNFANPDMVGHTGSLSAAIKACETVDQGLGRINDAIVKAGGVLLVTADHGNC 445
[Close]
BLAST of EY736121 vs. ExPASy Swiss-Prot
Match: GPMI_SHEAM (2,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Shewanella amazonensis (strain ATCC BAA-1098 / SB2B) GN=gpmI PE=3 SV=1)

HSP 1 Score: 48.521 bits (114), Expect = 1.231e-13
Identity = 26/72 (36.11%), Postives = 41/72 (56.94%), Query Frame = 3 
Query:   99 GEYVVHNGVRTFACSETVKFGHVTCFWNGNRSGYFDSNLEEYVEIPSDSGITFNVQPTMKALEIADRAKKAI 314
            GE + ++G      SET K+ HVT F+NG +   FD   E+ + I S    T+++QP M + E+ D+  +AI
Sbjct:  317 GEVLQNSGKTQLRISETEKYAHVTFFFNGGKEQPFDG--EDRILINSPKVATYDLQPEMSSTELTDKLVEAI 386

HSP 2 Score: 46.9802 bits (110), Expect = 1.231e-13
Identity = 22/46 (47.83%), Postives = 29/46 (63.04%), Query Frame = 1 
Query:  346 NLPNIDMVVHTGDIEADVVACNAADNTVKIIIDAIHKFGGVYLVEA 483
            N PN DMV HTG+ +A V AC A D  +  ++DA+ K GG  L+ A
Sbjct:  397 NYPNGDMVGHTGNFDAAVKACEAVDTCIGRVVDALAKVGGECLITA 442
[Close]
BLAST of EY736121 vs. ExPASy Swiss-Prot
Match: GPMI_PSEMY (2,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Pseudomonas mendocina (strain ymp) GN=gpmI PE=3 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 2.699e-13
Identity = 35/104 (33.65%), Postives = 53/104 (50.96%), Query Frame = 3 
Query:    3 PRGFFFFIYKKXNNVRXLAGTGPESRGSTTSSGEYVVHNGVRTFACSETVKFGHVTCFWNGNRSGYFDSNLEEYVEIPSDSGITFNVQPTMKALEIADRAKKAI 314
            P+  F  + +   ++   A   PE+   T   GEY+  NG      +ET K+ HVT F++G R   F+   EE + IPS    T++++P M A E+ DR   AI
Sbjct:  284 PQVGFVMLTQYAASIPAPAAFAPEAL--TNVLGEYLAKNGKTQLRIAETEKYAHVTFFFSGGREEPFEG--EERILIPSPDVATYDLKPEMSAPEVTDRIVDAI 383

HSP 2 Score: 39.2762 bits (90), Expect = 2.699e-13
Identity = 23/55 (41.82%), Postives = 28/55 (50.91%), Query Frame = 1 
Query:  319 NPTFHQVRVNLPNIDMVVHTGDIEADVVACNAADNTVKIIIDAIHKFGGVYLVEA 483
            N  +  + VN  N DMV HTG  EA V A    D  V  I +A+ K GG  L+ A
Sbjct:  385 NQRYDVIIVNYANGDMVGHTGVFEAAVKAVETLDTCVGRITEALQKVGGEALITA 439
[Close]
BLAST of EY736121 vs. ExPASy Swiss-Prot
Match: GPMI_SALRD (2,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Salinibacter ruber (strain DSM 13855) GN=gpmI PE=3 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 4.549e-13
Identity = 29/73 (39.73%), Postives = 42/73 (57.53%), Query Frame = 3 
Query:   99 GEYVVHNGVRTFACSETVKFGHVTCFWNGNRSGYFDSNLEEYVEIPSDSGITFNVQPTMKALEIADRAKKAIR 317
            GE +   G R    +ET K+ HVT F++G R   FD   E+ V +PS    T++ QP M A E+ADR  +++R
Sbjct:  323 GEVLSARGGRQLRAAETEKYAHVTYFFSGGREAPFDG--EDRVLVPSPKVDTYDQQPEMSAPELADRVSRSLR 393

HSP 2 Score: 39.2762 bits (90), Expect = 4.549e-13
Identity = 18/40 (45.00%), Postives = 23/40 (57.50%), Query Frame = 1 
Query:  343 VNLPNIDMVVHTGDIEADVVACNAADNTVKIIIDAIHKFG 462
            +N  N DMV HTGD EA V AC A D   + +++A    G
Sbjct:  402 LNFANPDMVGHTGDFEAAVAACEAVDRGARQVVEAARDQG 441
[Close]
BLAST of EY736121 vs. ExPASy Swiss-Prot
Match: GPMI_PSEPF (2,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Pseudomonas fluorescens (strain Pf0-1) GN=gpmI PE=3 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 4.553e-13
Identity = 29/72 (40.28%), Postives = 41/72 (56.94%), Query Frame = 3 
Query:   99 GEYVVHNGVRTFACSETVKFGHVTCFWNGNRSGYFDSNLEEYVEIPSDSGITFNVQPTMKALEIADRAKKAI 314
            G+Y+  NG      +ET K+ HVT F++G R   F    EE + IPS    T+++QP M A E+ DR  +AI
Sbjct:  314 GDYLAKNGKTQLRIAETEKYAHVTFFFSGGREEPFPG--EERILIPSPKVATYDLQPEMSAPEVTDRIVEAI 383

HSP 2 Score: 39.2762 bits (90), Expect = 4.553e-13
Identity = 22/55 (40.00%), Postives = 29/55 (52.73%), Query Frame = 1 
Query:  319 NPTFHQVRVNLPNIDMVVHTGDIEADVVACNAADNTVKIIIDAIHKFGGVYLVEA 483
            N  +  + VN  N DMV H+G  EA V A    D  V  I++A+ K GG  L+ A
Sbjct:  385 NQRYDVIVVNYANGDMVGHSGVFEAAVKAVECLDTCVGRIVEALEKVGGEALITA 439
[Close]
BLAST of EY736121 vs. ExPASy Swiss-Prot
Match: GPMI_METFK (2,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) GN=gpmI PE=3 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 5.910e-13
Identity = 28/72 (38.89%), Postives = 42/72 (58.33%), Query Frame = 3 
Query:   99 GEYVVHNGVRTFACSETVKFGHVTCFWNGNRSGYFDSNLEEYVEIPSDSGITFNVQPTMKALEIADRAKKAI 314
            GEYV  +G++    +ET K+ HVT F+NG     F    E+ + +PS    T+++QP M A E+ DR + AI
Sbjct:  318 GEYVAQHGLKQLRIAETEKYPHVTFFFNGGEEQVFAG--EDRILVPSPKVATYDLQPEMSAYEVTDRLEAAI 387

HSP 2 Score: 36.5798 bits (83), Expect = 5.910e-13
Identity = 16/46 (34.78%), Postives = 24/46 (52.17%), Query Frame = 1 
Query:  346 NLPNIDMVVHTGDIEADVVACNAADNTVKIIIDAIHKFGGVYLVEA 483
            N  N DMV H+GD+ A + A    D  +  I+ A+   GG  ++ A
Sbjct:  398 NYANCDMVGHSGDLSASIKAVETIDTCIARIVKAMESIGGQMIITA 443
The following BLAST results are available for this feature:
BLAST of EY736121 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 63
Match NameE-valueIdentityDescription
GPMI_PSEP15.326e-1541.672,3-bisphosphoglycerate-independent phosphoglycera... [more]
GPMI_PSEPK6.920e-1541.672,3-bisphosphoglycerate-independent phosphoglycera... [more]
GPMI_PSEPG1.168e-1440.282,3-bisphosphoglycerate-independent phosphoglycera... [more]
GPMI_PSEE41.972e-1441.672,3-bisphosphoglycerate-independent phosphoglycera... [more]
GPMI_GLUOX5.619e-1440.282,3-bisphosphoglycerate-independent phosphoglycera... [more]
GPMI_SHEAM1.231e-1336.112,3-bisphosphoglycerate-independent phosphoglycera... [more]
GPMI_PSEMY2.699e-1333.652,3-bisphosphoglycerate-independent phosphoglycera... [more]
GPMI_SALRD4.549e-1339.732,3-bisphosphoglycerate-independent phosphoglycera... [more]
GPMI_PSEPF4.553e-1340.282,3-bisphosphoglycerate-independent phosphoglycera... [more]
GPMI_METFK5.910e-1338.892,3-bisphosphoglycerate-independent phosphoglycera... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-705-012-C01-CT.F Sweet orange fruit, development stadium (6 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY736121 ID=EY736121; Name=EY736121; organism=Citrus sinensis; type=EST; length=972bp
TGCCCCGTGGCTTTTTTTTTTTTATTTATAAAAAAANGAATAACGTCCGT
ANCCTTGCAGGTACTGGTCCGGAATCCCGTGGATCGACCACGTCGTCCGG
TGAATATGTAGTGCATAATGGTGTCCGTACTTTTGCTTGCAGTGAGACTG
TCAAGTTTGGACATGTCACTTGCTTCTGGAATGGAAACCGCTCTGGGTAT
TTCGACTCGAATTTGGAGGAATATGTGGAAATTCCTAGTGATTCTGGAAT
TACTTTCAATGTACAGCCCACAATGAAGGCTTTGGAGATTGCTGACAGGG
CAAAGAAGGCCATCCGTTAATCCCACGTTCCACCAGGTACGTGTGAACCT
ACCAAATATTGACATGGTGGTGCACACCGGAGATATTGAGGCAGACGTGG
TGGCATGCAATGCTGCTGATAACACTGTCAAAATAATCATTGATGCAATA
CACAAATTTGGTGGAGTATATCTTGTGGAAGCAGGATCATGGCTATGCTG
ACGACATGGTTCGTAAGGAACCCAGTCGGGCGAGGCCCTCCTTCCGGATG
GCATACTTCCCCTCGTCGCGTCCTCACCGCCGTACCCCCCCTCCGGTGCA
TTCCCCGGTCCTCGAGGGCTCAATCCCTTCAGCTCCCCGTTCCGATCGCT
CCTCCGGGGGGGCTCGGCACCCCTGCCAGGAGAATGGGCCCATACCAAGC
GGACTACCTAGTAGGTTAGTACGGCCCAGCCCCGGCCCGCTCGCGCCCTC
GGCCCCCCGCCTCCCCCCTCCACCCCCGGCCCTCCGTCGCACCCCCCCCC
CCCACCCCTCCCGCCCCGCCCACCCCCCCCCCCCCGCCCCCCCCCCCCCC
TCTCGCCCGCCACCCGCCCCCCCCCCCCCCCCCCCCCCACCTCTCGGCTC
CCCCCCCCCCTCACAACCTCCACCCCCCCCCGACCAAACTCTCAACCCGC
CTCCACGCCCCTAATCACACAC
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