EY735758

Overview
NameEY735758
Unique NameEY735758
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length464
Libraries
Library NameType
Sweet orange fruit, development stadium (6 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_ACICJ (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Acidiphilium cryptum (strain JF-5) GN=metE PE=3 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 8.820e-18
Identity = 50/106 (47.17%), Postives = 63/106 (59.43%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSL-GI*ACFYLDWGVPPFEITTVVVRN 316
            P+ MTV W+  AQS+TARP+KGMLTGP  +L W FVR++ PR      IALAL+DE+ DLE      I + +   REG  L       YL W V  F + T VVR+
Sbjct:  538 PRPMTVRWARHAQSLTARPVKGMLTGPVTMLQWSFVRDDLPRETVCRQIALALRDEVADLEAAGLAIIQVDEPAFREGLPLRTADREAYLRWAVSCFRLATAVVRD 643

HSP 2 Score: 23.0978 bits (48), Expect = 8.820e-18
Identity = 10/15 (66.67%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  363 PPIYTLDADVIPIET 407
            P I T+DAD I IET
Sbjct:  661 PAIATMDADAISIET 675
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_XYLFA (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Xylella fastidiosa GN=metE PE=3 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 8.822e-18
Identity = 50/106 (47.17%), Postives = 65/106 (61.32%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSLGI*A-CFYLDWGVPPFEITTVVVRN 316
            P  MTV WS+ AQS+T +PMKGMLTGP  +L W FVR++  R +    IALAL+DE+QDLEK     I + +  +REG  L       YL+W V  F I +  VR+
Sbjct:  534 PAPMTVTWSAYAQSLTDKPMKGMLTGPVTMLQWSFVRDDQERAQTCRQIALALRDEVQDLEKAGIKVIQIDEPAIREGLPLRRGEWADYLNWAVESFRIASSNVRD 639

HSP 2 Score: 20.7866 bits (42), Expect = 8.822e-18
Identity = 9/13 (69.23%), Postives = 10/13 (76.92%), Query Frame = 3 
Query:  369 IYTLDADVIPIET 407
            +  LDADVI IET
Sbjct:  659 VAALDADVISIET 671
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_BURCM (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) GN=metE PE=3 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.088e-17
Identity = 50/100 (50.00%), Postives = 63/100 (63.00%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSLGI*AC-FYLDWGVPPFEIT 298
            PKAMTV W + AQS+T +PMKGMLTGP  IL+W FVR++ PR  +   +ALA++DE+ DLEK     I + +  LREG  L       YL W V  F IT
Sbjct:  538 PKAMTVEWITYAQSLTNKPMKGMLTGPVTILNWSFVRDDQPRSVSCYQLALAIRDEVLDLEKAGVRVIQIDEAALREGLPLRRAQWGEYLKWAVEAFRIT 637
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_BURA4 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia ambifaria (strain MC40-6) GN=metE PE=3 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.088e-17
Identity = 50/100 (50.00%), Postives = 63/100 (63.00%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSLGI*AC-FYLDWGVPPFEIT 298
            PKAMTV W + AQS+T +PMKGMLTGP  IL+W FVR++ PR  +   +ALA++DE+ DLEK     I + +  LREG  L       YL W V  F IT
Sbjct:  538 PKAMTVEWITYAQSLTNKPMKGMLTGPVTILNWSFVRDDQPRSVSCYQLALAIRDEVLDLEKAGVRVIQIDEAALREGLPLRRAQWGEYLKWAVEAFRIT 637
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_RHOPT (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Rhodopseudomonas palustris (strain TIE-1) GN=metE PE=3 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 1.146e-17
Identity = 51/106 (48.11%), Postives = 65/106 (61.32%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSLGI*AC-FYLDWGVPPFEITTVVVRN 316
            PK MTV W   AQS+T RPMKGMLTGP  IL+W FVR++ PR      IALA++DE+ DLE+   T I + +  LREG  L       YLDW V  F + +  V++
Sbjct:  561 PKPMTVGWWKYAQSLTGRPMKGMLTGPVTILNWSFVRDDVPRSTACLQIALAIRDEVGDLEQAGATMIQIDEAALREGLPLRRSEWKGYLDWAVECFRLCSSGVKD 666

HSP 2 Score: 20.4014 bits (41), Expect = 1.146e-17
Identity = 9/13 (69.23%), Postives = 10/13 (76.92%), Query Frame = 3 
Query:  369 IYTLDADVIPIET 407
            I  +DADVI IET
Sbjct:  686 IAAMDADVISIET 698
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_RHOPA (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Rhodopseudomonas palustris GN=metE PE=3 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 1.146e-17
Identity = 51/106 (48.11%), Postives = 65/106 (61.32%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSLGI*AC-FYLDWGVPPFEITTVVVRN 316
            PK MTV W   AQS+T RPMKGMLTGP  IL+W FVR++ PR      IALA++DE+ DLE+   T I + +  LREG  L       YLDW V  F + +  V++
Sbjct:  561 PKPMTVGWWKYAQSLTGRPMKGMLTGPVTILNWSFVRDDVPRSTACLQIALAIRDEVGDLEQAGATMIQIDEAALREGLPLRRSEWKGYLDWAVECFRLCSSGVKD 666

HSP 2 Score: 20.4014 bits (41), Expect = 1.146e-17
Identity = 9/13 (69.23%), Postives = 10/13 (76.92%), Query Frame = 3 
Query:  369 IYTLDADVIPIET 407
            I  +DADVI IET
Sbjct:  686 IAAMDADVISIET 698
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_NEIMF (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Neisseria meningitidis serogroup C / serotype 2a (strain ATCC 700532 / FAM18) GN=metE PE=3 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 1.422e-17
Identity = 65/154 (42.21%), Postives = 81/154 (52.60%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSL-GI*ACFYLDWGVPPFEITTVVVRNYPDSPPI----WYSNFKKFPPHL-YIGC*CDPH*DSRSDEKLLSVFRE 445
            P+AMTV WS+ AQS+T RPMKGMLTGP  IL W FVRN+ PR      IALAL DE+ DLEK     I + +  +REG  L       YL+W    F +++       DS  I     YS F    P +  +         SRSD +LL+ F E
Sbjct:  531 PEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFVRNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAGESFRLSSA---GCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGE 681
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_NEIMB (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Neisseria meningitidis serogroup B GN=metE PE=1 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 1.422e-17
Identity = 65/154 (42.21%), Postives = 81/154 (52.60%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSL-GI*ACFYLDWGVPPFEITTVVVRNYPDSPPI----WYSNFKKFPPHL-YIGC*CDPH*DSRSDEKLLSVFRE 445
            P+AMTV WS+ AQS+T RPMKGMLTGP  IL W FVRN+ PR      IALAL DE+ DLEK     I + +  +REG  L       YL+W    F +++       DS  I     YS F    P +  +         SRSD +LL+ F E
Sbjct:  531 PEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFVRNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAGESFRLSSA---GCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGE 681
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_NEIMA (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Neisseria meningitidis serogroup A GN=metE PE=3 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 1.422e-17
Identity = 65/154 (42.21%), Postives = 81/154 (52.60%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSL-GI*ACFYLDWGVPPFEITTVVVRNYPDSPPI----WYSNFKKFPPHL-YIGC*CDPH*DSRSDEKLLSVFRE 445
            P+AMTV WS+ AQS+T RPMKGMLTGP  IL W FVRN+ PR      IALAL DE+ DLEK     I + +  +REG  L       YL+W    F +++       DS  I     YS F    P +  +         SRSD +LL+ F E
Sbjct:  531 PEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFVRNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAGESFRLSST---GCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGE 681
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_NITWN (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Nitrobacter winogradskyi (strain Nb-255 / ATCC 25391) GN=metE PE=3 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 1.938e-17
Identity = 49/99 (49.49%), Postives = 62/99 (62.63%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSLGI*AC-FYLDWGVPPFEI 295
            P+ MTV W   AQS+TA+PMKGMLTGP  IL+W FVR++ PR      IALA++DE+ DLE+   T I + +  LREG  L       YLDW V  F +
Sbjct:  549 PRPMTVGWWKYAQSLTAKPMKGMLTGPVTILNWSFVRDDVPRSLACRQIALAIRDEVSDLEQAGATMIQIDEAALREGLPLRRSEWKTYLDWAVECFRL 647

HSP 2 Score: 20.7866 bits (42), Expect = 1.938e-17
Identity = 9/13 (69.23%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:  369 IYTLDADVIPIET 407
            I ++DADVI IET
Sbjct:  674 IASMDADVISIET 686
The following BLAST results are available for this feature:
BLAST of EY735758 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 242
Match NameE-valueIdentityDescription
METE_ACICJ8.820e-1847.175-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_XYLFA8.822e-1847.175-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURCM1.088e-1750.005-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURA41.088e-1750.005-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_RHOPT1.146e-1748.115-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_RHOPA1.146e-1748.115-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_NEIMF1.422e-1742.215-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_NEIMB1.422e-1742.215-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_NEIMA1.422e-1742.215-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_NITWN1.938e-1749.495-methyltetrahydropteroyltriglutamate--homocystein... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-705-004-F09-CT.F Sweet orange fruit, development stadium (6 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY735758 ID=EY735758; Name=EY735758; organism=Citrus sinensis; type=EST; length=464bp
TCCCAAGGCTATGACCGTCTTCTGGTCCTCCTTGGCTCAGAGCATGACTG
CTCGGCCAATGAAGGGAATGCTTACTGGCCCTGGTCACATTCTCCACTGG
GTCTTTGTCAGAAATGAGCCGCCCCGACATGAAAACTGGTAACACATTGC
TTTGGCCCTCCAGGACGAAATCCAGGATCTTGAGAAAGGGCGGTTTACGT
TTATCCCATTGATTAAGGTGCCCTTAAGGGAAGGATGCTCCTTAGGAATC
TGAGCCTGTTTCTACTTGGACTGGGGAGTCCCTCCTTTCGAAATCACCAC
TGTGGTGGTCCGAAACTACCCCGATTCCCCCCCCATTTGGTATTCCAATT
TCAAGAAATTTCCCCCCCATTTATACATTGGATGCTGATGTGATCCCCAT
TGAGACTCTCGTTCTGATGAGAAGCTCCTGTCTGTCTTCCGTGAGGGAGT
CANGTATGGTGCTG
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