EY735758

Overview
NameEY735758
Unique NameEY735758
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length464
Libraries
Library NameType
Sweet orange fruit, development stadium (6 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_BORA1 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Bordetella avium (strain 197N) GN=metE PE=3 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 1.938e-17
Identity = 50/104 (48.08%), Postives = 63/104 (60.58%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSL-GI*ACFYLDWGVPPFEITTVVV 310
            P  MTV WSS AQS+T +P+KGMLTGP  IL W FVR++ PR      +ALAL+DE+ DLEK     I + +  +REG  L       YLDW V  F ++T  V
Sbjct:  540 PAPMTVGWSSYAQSLTDKPVKGMLTGPVTILQWSFVRDDQPREATCRQLALALRDEVLDLEKAGVKVIQIDEPAIREGLPLRRADWQAYLDWAVDCFRLSTAGV 643

HSP 2 Score: 20.4014 bits (41), Expect = 1.938e-17
Identity = 9/13 (69.23%), Postives = 10/13 (76.92%), Query Frame = 3 
Query:  369 IYTLDADVIPIET 407
            I  +DADVI IET
Sbjct:  665 IAAMDADVITIET 677
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_MARAV (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Marinobacter aquaeolei (strain ATCC 700491 / DSM 11845 / VT8) GN=metE PE=3 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 2.425e-17
Identity = 46/99 (46.46%), Postives = 64/99 (64.65%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSL-GI*ACFYLDWGVPPFEI 295
            P+AMTV W   AQS+T +P+KGMLTGP  IL+W FVR++ PR ++   +ALA+++E+QDLEK     I + +  LREG  L       YL+W +  F I
Sbjct:  533 PQAMTVEWIRYAQSLTDKPVKGMLTGPVTILNWSFVRDDQPRKDSCLQLALAIREEVQDLEKAGVNIIQIDEAALREGLPLRQSDWATYLEWAINSFRI 631
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_BURCJ (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia cepacia (strain J2315 / LMG 16656) GN=metE PE=3 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 2.425e-17
Identity = 49/100 (49.00%), Postives = 63/100 (63.00%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSL-GI*ACFYLDWGVPPFEIT 298
            PKAMTV W + AQS+T +PMKGMLTGP  IL+W FVR++ PR  +   +ALA++DE+ DLE+     I + +  LREG  L       YL W V  F IT
Sbjct:  538 PKAMTVEWITYAQSLTNKPMKGMLTGPVTILNWSFVRDDQPRSVSCYQLALAIRDEVLDLEQAGVRVIQIDEAALREGLPLRRAQWSEYLKWAVESFRIT 637
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_BURCH (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia cenocepacia (strain HI2424) GN=metE PE=3 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 2.425e-17
Identity = 49/100 (49.00%), Postives = 63/100 (63.00%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSL-GI*ACFYLDWGVPPFEIT 298
            PKAMTV W + AQS+T +PMKGMLTGP  IL+W FVR++ PR  +   +ALA+++E+ DLEK     I + +  LREG  L       YL W V  F IT
Sbjct:  538 PKAMTVEWIAYAQSLTRKPMKGMLTGPVTILNWSFVRDDQPRAVSCYQLALAIREEVLDLEKAGVRVIQIDEAALREGLPLRRAQWSEYLKWAVESFRIT 637
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_BURCC (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia cenocepacia (strain MC0-3) GN=metE PE=3 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 2.425e-17
Identity = 49/100 (49.00%), Postives = 63/100 (63.00%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSL-GI*ACFYLDWGVPPFEIT 298
            PKAMTV W + AQS+T +PMKGMLTGP  IL+W FVR++ PR  +   +ALA+++E+ DLEK     I + +  LREG  L       YL W V  F IT
Sbjct:  538 PKAMTVEWIAYAQSLTRKPMKGMLTGPVTILNWSFVRDDQPRAVSCYQLALAIREEVLDLEKAGVRVIQIDEAALREGLPLRRAQWSEYLKWAVESFRIT 637
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_BURCA (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia cenocepacia (strain AU 1054) GN=metE PE=3 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 2.425e-17
Identity = 49/100 (49.00%), Postives = 63/100 (63.00%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSL-GI*ACFYLDWGVPPFEIT 298
            PKAMTV W + AQS+T +PMKGMLTGP  IL+W FVR++ PR  +   +ALA+++E+ DLEK     I + +  LREG  L       YL W V  F IT
Sbjct:  538 PKAMTVEWIAYAQSLTRKPMKGMLTGPVTILNWSFVRDDQPRAVSCYQLALAIREEVLDLEKAGVRVIQIDEAALREGLPLRRAQWSEYLKWAVESFRIT 637
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_BORPD (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Bordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448) GN=metE PE=3 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 2.520e-17
Identity = 49/104 (47.12%), Postives = 64/104 (61.54%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSL-GI*ACFYLDWGVPPFEITTVVV 310
            P  MTV WSS AQS+T +P+KGMLTGP  IL W FVR++ PR +    +ALAL+DE+ DLE+     I + +  +REG  L       YLDW V  F ++T  V
Sbjct:  540 PAPMTVGWSSYAQSLTDKPVKGMLTGPVTILQWSFVRDDQPREQTCRQLALALRDEVVDLEQAGVRVIQIDEPAIREGLPLRRADWQAYLDWAVDCFRLSTAGV 643

HSP 2 Score: 20.4014 bits (41), Expect = 2.520e-17
Identity = 9/13 (69.23%), Postives = 10/13 (76.92%), Query Frame = 3 
Query:  369 IYTLDADVIPIET 407
            I  +DADVI IET
Sbjct:  665 IAAMDADVITIET 677
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_BURS3 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia sp. (strain 383) GN=metE PE=3 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 3.167e-17
Identity = 49/100 (49.00%), Postives = 63/100 (63.00%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSLGI*AC-FYLDWGVPPFEIT 298
            PKAMTV W + AQS+T +PMKGMLTGP  IL+W FVR++ PR  +   +ALA+++E+ DLEK     I + +  LREG  L       YL W V  F IT
Sbjct:  538 PKAMTVEWITYAQSLTNKPMKGMLTGPVTILNWSFVRDDQPRSVSCYQLALAIREEVLDLEKAGVRVIQIDEAALREGLPLRRAQWGEYLKWAVESFRIT 637
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_XYLFT (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Xylella fastidiosa (strain Temecula1 / ATCC 700964) GN=metE PE=3 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 3.276e-17
Identity = 48/101 (47.52%), Postives = 62/101 (61.39%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSLGI*A-CFYLDWGVPPFEITT 301
            P  MTV WS+ AQS+T +PMKGMLTGP  +L W FVR++  R +    IALAL+DE+QDLEK     I + +  +REG  L       YL+W V  F I +
Sbjct:  534 PAPMTVTWSAYAQSLTDKPMKGMLTGPVTMLQWSFVRDDQERAQTCRQIALALRDEVQDLEKAGIKVIQIDEPAIREGLPLRRGEWADYLNWAVESFRIAS 634

HSP 2 Score: 20.7866 bits (42), Expect = 3.276e-17
Identity = 9/13 (69.23%), Postives = 10/13 (76.92%), Query Frame = 3 
Query:  369 IYTLDADVIPIET 407
            +  LDADVI IET
Sbjct:  659 VAALDADVISIET 671
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_XYLFM (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Xylella fastidiosa (strain M12) GN=metE PE=3 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 3.276e-17
Identity = 48/101 (47.52%), Postives = 62/101 (61.39%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSLGI*A-CFYLDWGVPPFEITT 301
            P  MTV WS+ AQS+T +PMKGMLTGP  +L W FVR++  R +    IALAL+DE+QDLEK     I + +  +REG  L       YL+W V  F I +
Sbjct:  534 PAPMTVTWSAYAQSLTDKPMKGMLTGPVTMLQWSFVRDDQERAQTCRQIALALRDEVQDLEKAGIKVIQIDEPAIREGLPLRRGEWADYLNWAVESFRIAS 634

HSP 2 Score: 20.7866 bits (42), Expect = 3.276e-17
Identity = 9/13 (69.23%), Postives = 10/13 (76.92%), Query Frame = 3 
Query:  369 IYTLDADVIPIET 407
            +  LDADVI IET
Sbjct:  659 VAALDADVISIET 671
The following BLAST results are available for this feature:
BLAST of EY735758 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 242
Match NameE-valueIdentityDescription
METE_BORA11.938e-1748.085-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_MARAV2.425e-1746.465-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURCJ2.425e-1749.005-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURCH2.425e-1749.005-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURCC2.425e-1749.005-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURCA2.425e-1749.005-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BORPD2.520e-1747.125-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURS33.167e-1749.005-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_XYLFT3.276e-1747.525-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_XYLFM3.276e-1747.525-methyltetrahydropteroyltriglutamate--homocystein... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-705-004-F09-CT.F Sweet orange fruit, development stadium (6 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY735758 ID=EY735758; Name=EY735758; organism=Citrus sinensis; type=EST; length=464bp
TCCCAAGGCTATGACCGTCTTCTGGTCCTCCTTGGCTCAGAGCATGACTG
CTCGGCCAATGAAGGGAATGCTTACTGGCCCTGGTCACATTCTCCACTGG
GTCTTTGTCAGAAATGAGCCGCCCCGACATGAAAACTGGTAACACATTGC
TTTGGCCCTCCAGGACGAAATCCAGGATCTTGAGAAAGGGCGGTTTACGT
TTATCCCATTGATTAAGGTGCCCTTAAGGGAAGGATGCTCCTTAGGAATC
TGAGCCTGTTTCTACTTGGACTGGGGAGTCCCTCCTTTCGAAATCACCAC
TGTGGTGGTCCGAAACTACCCCGATTCCCCCCCCATTTGGTATTCCAATT
TCAAGAAATTTCCCCCCCATTTATACATTGGATGCTGATGTGATCCCCAT
TGAGACTCTCGTTCTGATGAGAAGCTCCTGTCTGTCTTCCGTGAGGGAGT
CANGTATGGTGCTG
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