EY735758

Overview
NameEY735758
Unique NameEY735758
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length464
Libraries
Library NameType
Sweet orange fruit, development stadium (6 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_BURM9 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia mallei (strain NCTC 10229) GN=metE PE=3 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 4.258e-17
Identity = 48/99 (48.48%), Postives = 60/99 (60.61%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSL-GI*ACFYLDWGVPPFEI 295
            P+ MTV W+S AQS++A+P+KGMLTGP  +L W FVR++ PR      IALAL+ E  DLEK     I + +  LREG  L       YLDW V  F I
Sbjct:  533 PEPMTVGWASYAQSLSAKPVKGMLTGPVTMLQWSFVRDDQPRATTALQIALALRQETLDLEKAGIGMIQIDEPALREGLPLKARERAAYLDWAVRAFGI 631

HSP 2 Score: 23.483 bits (49), Expect = 4.258e-17
Identity = 11/15 (73.33%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  363 PPIYTLDADVIPIET 407
            P I  LDADVI IET
Sbjct:  656 PSIAALDADVISIET 670
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_BURM7 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia mallei (strain NCTC 10247) GN=metE PE=3 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 4.258e-17
Identity = 48/99 (48.48%), Postives = 60/99 (60.61%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSL-GI*ACFYLDWGVPPFEI 295
            P+ MTV W+S AQS++A+P+KGMLTGP  +L W FVR++ PR      IALAL+ E  DLEK     I + +  LREG  L       YLDW V  F I
Sbjct:  533 PEPMTVGWASYAQSLSAKPVKGMLTGPVTMLQWSFVRDDQPRATTALQIALALRQETLDLEKAGIGMIQIDEPALREGLPLKARERAAYLDWAVRAFGI 631

HSP 2 Score: 23.483 bits (49), Expect = 4.258e-17
Identity = 11/15 (73.33%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  363 PPIYTLDADVIPIET 407
            P I  LDADVI IET
Sbjct:  656 PSIAALDADVISIET 670
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_BURVG (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia vietnamiensis (strain G4 / LMG 22486) GN=metE PE=3 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 5.402e-17
Identity = 48/106 (45.28%), Postives = 65/106 (61.32%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSLGI*AC-FYLDWGVPPFEITTVVVRN 316
            P AMTV W + AQS+T++PMKGMLTGP  IL+W FVR++ PR  +   +ALA++DE+ DLE+     I + +  LREG  L       YL W +  F I    VR+
Sbjct:  538 PSAMTVEWIAYAQSLTSKPMKGMLTGPVTILNWSFVRDDQPRSVSCYQLALAIRDEVLDLERAGVRVIQIDEAALREGLPLRRAQWPAYLKWAIDAFRIAANGVRD 643
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_PARL1 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Parvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966) GN=metE PE=3 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 7.195e-17
Identity = 49/106 (46.23%), Postives = 62/106 (58.49%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSLGI*AC-FYLDWGVPPFEITTVVVRN 316
            P+ MTV WS  AQS+T RP+KGMLTGP  IL W FVR++ PR E    IALA++DE+ DLE      I + +  LREG  L       YL W    F ++  V R+
Sbjct:  536 PEPMTVEWSRYAQSLTDRPVKGMLTGPVTILQWSFVRDDQPRSETCRQIALAIRDEVYDLEAAGLGMIQIDEPALREGLPLHRSEWDTYLRWACECFRLSVCVARD 641

HSP 2 Score: 20.7866 bits (42), Expect = 7.195e-17
Identity = 10/13 (76.92%), Postives = 10/13 (76.92%), Query Frame = 3 
Query:  369 IYTLDADVIPIET 407
            I  LDADVI IET
Sbjct:  661 IADLDADVISIET 673
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_CHRVO (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Chromobacterium violaceum GN=metE PE=3 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 9.353e-17
Identity = 47/101 (46.53%), Postives = 60/101 (59.41%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSL-GI*ACFYLDWGVPPFEITT 301
            PK MTV W+  AQS+TA+P+KGMLTGP  IL W FVR++ PR E    IALAL DE+ DLE      I + +  +REG  L       YL W    F +++
Sbjct:  531 PKPMTVDWARYAQSLTAKPVKGMLTGPVTILQWSFVRDDLPRREVCRQIALALNDEVLDLEAAGIRVIQIDEPAIREGLPLKRAGRDVYLAWAGEAFRLSS 631

HSP 2 Score: 23.483 bits (49), Expect = 9.353e-17
Identity = 11/15 (73.33%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  363 PPIYTLDADVIPIET 407
            P I  LDADVI IET
Sbjct:  654 PAIAALDADVITIET 668
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_BURP8 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia phymatum (strain DSM 17167 / STM815) GN=metE PE=3 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 1.203e-16
Identity = 48/99 (48.48%), Postives = 61/99 (61.62%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSLGI*AC-FYLDWGVPPFEI 295
            PKAMTV W   AQ+ TA+PMKGMLTGP  IL+W FVR++ PR  +   +ALA+++E+ DLEK     I + +  LREG  L       YL W V  F I
Sbjct:  538 PKAMTVEWICYAQAQTAKPMKGMLTGPVTILNWSFVRDDQPRSVSCRQLALAIREEVLDLEKAGVRVIQIDEAALREGLPLRRSQWNEYLQWAVESFRI 636
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_SACD2 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=metE PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 1.216e-16
Identity = 48/104 (46.15%), Postives = 64/104 (61.54%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSLGI*AC-FYLDWGVPPFEITTVVV 310
            P+ +TV W+  AQS+T++P+KGMLTGP  +L W FVR++ PR      IALAL+ E+ DLE    T I + +  LREG  L       YLDW V  F+I+T  V
Sbjct:  539 PRKITVDWAEYAQSLTSKPVKGMLTGPITMLQWSFVRDDQPRKITAQQIALALRSEVNDLEAAGITIIQVDEPALREGLPLRKKDWQNYLDWAVEAFKISTAGV 642

HSP 2 Score: 20.7866 bits (42), Expect = 1.216e-16
Identity = 10/13 (76.92%), Postives = 10/13 (76.92%), Query Frame = 3 
Query:  369 IYTLDADVIPIET 407
            I  LDADVI IET
Sbjct:  664 IADLDADVITIET 676
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_BORPE (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Bordetella pertussis GN=metE PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 1.580e-16
Identity = 48/104 (46.15%), Postives = 62/104 (59.62%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSL-GI*ACFYLDWGVPPFEITTVVV 310
            P  MTV WS+ AQS+T +P+KGMLTGP  IL W FVR++ PR      +ALAL+DE+ DLE      I + +  +REG  L       YLDW V  F ++T  V
Sbjct:  540 PAPMTVGWSAYAQSLTDKPVKGMLTGPVTILQWSFVRDDQPREATCRQLALALRDEVVDLEAAGIRVIQIDEPAIREGLPLRRADWRAYLDWAVDCFRLSTAGV 643

HSP 2 Score: 20.4014 bits (41), Expect = 1.580e-16
Identity = 9/13 (69.23%), Postives = 10/13 (76.92%), Query Frame = 3 
Query:  369 IYTLDADVIPIET 407
            I  +DADVI IET
Sbjct:  665 IAAMDADVITIET 677
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_BORPA (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Bordetella parapertussis GN=metE PE=3 SV=2)

HSP 1 Score: 84.7297 bits (208), Expect = 1.580e-16
Identity = 48/104 (46.15%), Postives = 62/104 (59.62%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSL-GI*ACFYLDWGVPPFEITTVVV 310
            P  MTV WS+ AQS+T +P+KGMLTGP  IL W FVR++ PR      +ALAL+DE+ DLE      I + +  +REG  L       YLDW V  F ++T  V
Sbjct:  540 PAPMTVGWSAYAQSLTDKPVKGMLTGPVTILQWSFVRDDQPREATCRQLALALRDEVVDLEAAGIRVIQIDEPAIREGLPLRRADWRAYLDWAVDCFRLSTAGV 643

HSP 2 Score: 20.4014 bits (41), Expect = 1.580e-16
Identity = 9/13 (69.23%), Postives = 10/13 (76.92%), Query Frame = 3 
Query:  369 IYTLDADVIPIET 407
            I  +DADVI IET
Sbjct:  665 IAAMDADVITIET 677
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_BORBR (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Bordetella bronchiseptica GN=metE PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 1.580e-16
Identity = 48/104 (46.15%), Postives = 62/104 (59.62%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSL-GI*ACFYLDWGVPPFEITTVVV 310
            P  MTV WS+ AQS+T +P+KGMLTGP  IL W FVR++ PR      +ALAL+DE+ DLE      I + +  +REG  L       YLDW V  F ++T  V
Sbjct:  540 PAPMTVGWSAYAQSLTDKPVKGMLTGPVTILQWSFVRDDQPREATCRQLALALRDEVVDLEAAGIRVIQIDEPAIREGLPLRRADWRAYLDWAVDCFRLSTAGV 643

HSP 2 Score: 20.4014 bits (41), Expect = 1.580e-16
Identity = 9/13 (69.23%), Postives = 10/13 (76.92%), Query Frame = 3 
Query:  369 IYTLDADVIPIET 407
            I  +DADVI IET
Sbjct:  665 IAAMDADVITIET 677
The following BLAST results are available for this feature:
BLAST of EY735758 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 242
Match NameE-valueIdentityDescription
METE_BURM94.258e-1748.485-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURM74.258e-1748.485-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURVG5.402e-1745.285-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_PARL17.195e-1746.235-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_CHRVO9.353e-1746.535-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURP81.203e-1648.485-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_SACD21.216e-1646.155-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BORPE1.580e-1646.155-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BORPA1.580e-1646.155-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BORBR1.580e-1646.155-methyltetrahydropteroyltriglutamate--homocystein... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-705-004-F09-CT.F Sweet orange fruit, development stadium (6 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY735758 ID=EY735758; Name=EY735758; organism=Citrus sinensis; type=EST; length=464bp
TCCCAAGGCTATGACCGTCTTCTGGTCCTCCTTGGCTCAGAGCATGACTG
CTCGGCCAATGAAGGGAATGCTTACTGGCCCTGGTCACATTCTCCACTGG
GTCTTTGTCAGAAATGAGCCGCCCCGACATGAAAACTGGTAACACATTGC
TTTGGCCCTCCAGGACGAAATCCAGGATCTTGAGAAAGGGCGGTTTACGT
TTATCCCATTGATTAAGGTGCCCTTAAGGGAAGGATGCTCCTTAGGAATC
TGAGCCTGTTTCTACTTGGACTGGGGAGTCCCTCCTTTCGAAATCACCAC
TGTGGTGGTCCGAAACTACCCCGATTCCCCCCCCATTTGGTATTCCAATT
TCAAGAAATTTCCCCCCCATTTATACATTGGATGCTGATGTGATCCCCAT
TGAGACTCTCGTTCTGATGAGAAGCTCCTGTCTGTCTTCCGTGAGGGAGT
CANGTATGGTGCTG
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