EY735758

Overview
NameEY735758
Unique NameEY735758
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length464
Libraries
Library NameType
Sweet orange fruit, development stadium (6 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_HAES1 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Haemophilus somnus (strain 129Pt) GN=metE PE=3 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 1.330e-15
Identity = 59/149 (39.60%), Postives = 77/149 (51.68%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSL-GI*ACFYLDWGVPPFEITTV-VVRNYPDSPPIWYSNFKKFPPHL-YIGC*CDPH*DSRSDEKLLSVF 439
            PKAMTV WS  AQS+T + MKGMLTGP  IL W FVRN+ PR      I +AL DE+ DLEK     I + +  +REG  L       YL W    F ++++ V  +      + YS F    P +  +         SRSD +LL+ F
Sbjct:  531 PKAMTVRWSKYAQSLTKKVMKGMLTGPVTILQWSFVRNDIPRSTVCKQIGVALSDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDTYLQWAGEAFRLSSMGVADDTQIHTHMCYSEFNDILPAIAALDADVITIETSRSDMELLTAF 679
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_SERP5 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Serratia proteamaculans (strain 568) GN=metE PE=3 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 1.672e-15
Identity = 46/106 (43.40%), Postives = 63/106 (59.43%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSL-GI*ACFYLDWGVPPFEITTVVVRN 316
            P+A+TV W+  AQS+T +P+KGMLTGP  IL W F R +  R      IALAL+DE++DLEK     I + +  LREG  L       YL+W V  F++   V ++
Sbjct:  532 PEAITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPREDVTREVIAKQIALALRDEVEDLEKAGIGIIQIDEPALREGLPLRQSDWAAYLNWAVDAFKLNAAVAKD 637

HSP 2 Score: 21.1718 bits (43), Expect = 1.672e-15
Identity = 10/13 (76.92%), Postives = 10/13 (76.92%), Query Frame = 3 
Query:  369 IYTLDADVIPIET 407
            I  LDADVI IET
Sbjct:  657 IAALDADVITIET 669
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_KLEP7 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) GN=metE PE=3 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 1.672e-15
Identity = 47/106 (44.34%), Postives = 61/106 (57.55%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSLGI*AC-FYLDWGVPPFEITTVVVRN 316
            P+A+TV W+  AQS+TA+P+KGMLTGP  IL W F R +  R      IALAL+DE+ DLE      I + +  LREG  L       YL WGV  F +   V ++
Sbjct:  529 PQAITVDWAKYAQSLTAKPVKGMLTGPVTILCWSFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLKRSDWDAYLQWGVEAFRLNAAVAKD 634

HSP 2 Score: 21.1718 bits (43), Expect = 1.672e-15
Identity = 10/13 (76.92%), Postives = 10/13 (76.92%), Query Frame = 3 
Query:  369 IYTLDADVIPIET 407
            I  LDADVI IET
Sbjct:  654 IAALDADVITIET 666
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_LACLA (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Lactococcus lactis subsp. lactis GN=metE PE=3 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 1.738e-15
Identity = 50/129 (38.76%), Postives = 73/129 (56.59%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSLGI*ACF--YLDWGVPPFEITTVVVRNYPDS---PPIWYSNFKKFPPHL 373
            P+AMTV WS+ AQS T++P+KGMLTGP  IL+W F R +     +   +ALA+Q+E+ DLEK     I + +  LRE   L     +  YLDW +P F +    V+  P++     + YS F+   P +
Sbjct:  528 PEAMTVRWSAYAQSQTSKPVKGMLTGPVTILNWSFPREDISLKASTLQLALAVQEEVLDLEKAGIKIIQIDEAALREKLPLRKSDWYKEYLDWAIPAFRLVHSKVK--PETQIHTHMCYSEFEDIIPSI 654
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_ERWT9 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Erwinia tasmaniensis (strain DSM 17950 / Et1/99) GN=metE PE=3 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 2.172e-15
Identity = 47/110 (42.73%), Postives = 63/110 (57.27%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSL-----GI*ACFYLDWGVPPFEITTVVVRN 316
            P+A+TV W+  AQS+T +P+KGMLTGP  IL W F R +  R      IALAL+DE++DLEK     I + +  LREG  L     G     YL+W V  F +   V ++
Sbjct:  530 PEAITVNWAKYAQSLTDKPVKGMLTGPVTILCWSFPREDVSRETIAKQIALALRDEVEDLEKAGIGIIQIDEPALREGLPLHQSDWGA----YLEWAVDAFRLNAAVAQD 635

HSP 2 Score: 21.1718 bits (43), Expect = 2.172e-15
Identity = 10/13 (76.92%), Postives = 10/13 (76.92%), Query Frame = 3 
Query:  369 IYTLDADVIPIET 407
            I  LDADVI IET
Sbjct:  655 IAALDADVITIET 667
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_RALEU (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Ralstonia eutropha GN=metE PE=3 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 2.820e-15
Identity = 46/106 (43.40%), Postives = 63/106 (59.43%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSL-GI*ACFYLDWGVPPFEITTVVVRN 316
            P+ MTV  +  AQS+T RPMKGMLTGP  +L W FVR++ PR      +ALA++DE+ DLE+     I + +  LREG  L       YLDW V  F ++   V++
Sbjct:  530 PEPMTVDTARYAQSLTERPMKGMLTGPITMLQWSFVRDDQPRATTARQLALAIRDEVCDLEQAGIRVIQIDEPALREGLPLRRADWDAYLDWAVTAFRLSASGVQD 635

HSP 2 Score: 22.7126 bits (47), Expect = 2.820e-15
Identity = 10/15 (66.67%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  363 PPIYTLDADVIPIET 407
            P I  +DADVI IET
Sbjct:  653 PAIAAMDADVITIET 667
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_RALEH (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Ralstonia eutropha (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) GN=metE PE=3 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 2.820e-15
Identity = 46/106 (43.40%), Postives = 63/106 (59.43%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSL-GI*ACFYLDWGVPPFEITTVVVRN 316
            P+ MTV  +  AQS+T RPMKGMLTGP  +L W FVR++ PR      +ALA++DE+ DLE+     I + +  LREG  L       YLDW V  F ++   V++
Sbjct:  530 PEPMTVDTARYAQSLTERPMKGMLTGPITMLQWSFVRDDQPRATTARQLALAIRDEVCDLEQAGIRVIQIDEPALREGLPLRRADWDAYLDWAVTAFRLSASGVQD 635

HSP 2 Score: 22.7126 bits (47), Expect = 2.820e-15
Identity = 10/15 (66.67%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  363 PPIYTLDADVIPIET 407
            P I  +DADVI IET
Sbjct:  653 PAIAAMDADVITIET 667
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_BRAJA (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Bradyrhizobium japonicum GN=metE PE=3 SV=2)

HSP 1 Score: 80.4925 bits (197), Expect = 2.821e-15
Identity = 46/99 (46.46%), Postives = 60/99 (60.61%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSLGI*AC-FYLDWGVPPFEI 295
            PK +TV W   AQS+T++PMK MLTGP  IL+W FVR++ PR E    +ALA++DE++DLE      I + +  LREG  L       YLDW    F I
Sbjct:  551 PKPITVEWWRYAQSLTSKPMKAMLTGPVTILNWSFVRDDIPRSEVCRQLALAMRDEVRDLENAGAAMIQIDEAALREGLPLRRSDWKAYLDWAGDCFRI 649

HSP 2 Score: 20.4014 bits (41), Expect = 2.821e-15
Identity = 9/13 (69.23%), Postives = 10/13 (76.92%), Query Frame = 3 
Query:  369 IYTLDADVIPIET 407
            I  +DADVI IET
Sbjct:  676 IAAMDADVISIET 688
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_RHILO (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Rhizobium loti GN=metE PE=3 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 2.821e-15
Identity = 45/101 (44.55%), Postives = 60/101 (59.41%), Query Frame = 2 
Query:   11 MTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSL-GI*ACFYLDWGVPPFEITTVVV 310
            MT+ W   AQS+T +P+KGMLTGP  IL+W FVR++ PR      +ALA++DE+ DLEK     I + +  LREG  L       YLDW V  F + +  V
Sbjct:  551 MTLHWWRYAQSLTPKPVKGMLTGPVTILNWSFVRDDLPRSAVCRQVALAIRDEVSDLEKAGAKMIQIDEAALREGLPLREADWQAYLDWAVECFRLASTAV 651

HSP 2 Score: 20.4014 bits (41), Expect = 2.821e-15
Identity = 9/13 (69.23%), Postives = 10/13 (76.92%), Query Frame = 3 
Query:  369 IYTLDADVIPIET 407
            I  +DADVI IET
Sbjct:  673 IAAMDADVISIET 685
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_MESSB (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Mesorhizobium sp. (strain BNC1) GN=metE PE=3 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 2.822e-15
Identity = 46/105 (43.81%), Postives = 62/105 (59.05%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSL-GI*ACFYLDWGVPPFEITTVVVR 313
            P+ MTV W++ AQS+T +P+KGMLTGP  +L W FVR++ PR      IA AL+DE+ DLE      I + +  LREG  L       YLDW V  F + +  V+
Sbjct:  545 PQPMTVDWATYAQSLTDKPVKGMLTGPVTMLQWSFVRDDLPRETVCRQIAFALRDEVSDLEAAGIGVIQIDEPALREGLPLREAERPAYLDWAVECFRLASSGVK 649

HSP 2 Score: 20.4014 bits (41), Expect = 2.822e-15
Identity = 9/13 (69.23%), Postives = 10/13 (76.92%), Query Frame = 3 
Query:  369 IYTLDADVIPIET 407
            I  +DADVI IET
Sbjct:  670 IAAMDADVISIET 682
The following BLAST results are available for this feature:
BLAST of EY735758 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 242
Match NameE-valueIdentityDescription
METE_HAES11.330e-1539.605-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_SERP51.672e-1543.405-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_KLEP71.672e-1544.345-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_LACLA1.738e-1538.765-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_ERWT92.172e-1542.735-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_RALEU2.820e-1543.405-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_RALEH2.820e-1543.405-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BRAJA2.821e-1546.465-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_RHILO2.821e-1544.555-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_MESSB2.822e-1543.815-methyltetrahydropteroyltriglutamate--homocystein... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-705-004-F09-CT.F Sweet orange fruit, development stadium (6 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY735758 ID=EY735758; Name=EY735758; organism=Citrus sinensis; type=EST; length=464bp
TCCCAAGGCTATGACCGTCTTCTGGTCCTCCTTGGCTCAGAGCATGACTG
CTCGGCCAATGAAGGGAATGCTTACTGGCCCTGGTCACATTCTCCACTGG
GTCTTTGTCAGAAATGAGCCGCCCCGACATGAAAACTGGTAACACATTGC
TTTGGCCCTCCAGGACGAAATCCAGGATCTTGAGAAAGGGCGGTTTACGT
TTATCCCATTGATTAAGGTGCCCTTAAGGGAAGGATGCTCCTTAGGAATC
TGAGCCTGTTTCTACTTGGACTGGGGAGTCCCTCCTTTCGAAATCACCAC
TGTGGTGGTCCGAAACTACCCCGATTCCCCCCCCATTTGGTATTCCAATT
TCAAGAAATTTCCCCCCCATTTATACATTGGATGCTGATGTGATCCCCAT
TGAGACTCTCGTTCTGATGAGAAGCTCCTGTCTGTCTTCCGTGAGGGAGT
CANGTATGGTGCTG
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