EY735758

Overview
NameEY735758
Unique NameEY735758
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length464
Libraries
Library NameType
Sweet orange fruit, development stadium (6 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_ACTP7 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Actinobacillus pleuropneumoniae serotype 7 (strain AP76) GN=metE PE=3 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 8.624e-15
Identity = 59/152 (38.82%), Postives = 78/152 (51.32%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSL-GI*ACFYLDWGVPPFEITTVVVRNYPD-SPPIWYSNFKKFPPHLYIGC*CD--PH*DSRSDEKLLSVFRE 445
            P+ MTV WS  AQS+T + MKGMLTGP  IL W FVRN+ PR      I +AL DE+ DLEK     I + +  +REG  L       YL W    F ++ + V++       + YS F    P +  G   D      SRSD +LL+ F +
Sbjct:  530 PEPMTVRWSQYAQSLTDKVMKGMLTGPVTILQWSFVRNDIPRSTVCKQIGVALSDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLQWAGEAFRLSYMGVKDDTQIHTHMCYSEFNDILPAI-AGLDADVITIETSRSDMELLTAFAD 680
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_ACTP2 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Actinobacillus pleuropneumoniae serotype 5b (strain L20) GN=metE PE=3 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 8.624e-15
Identity = 59/152 (38.82%), Postives = 78/152 (51.32%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSL-GI*ACFYLDWGVPPFEITTVVVRNYPD-SPPIWYSNFKKFPPHLYIGC*CD--PH*DSRSDEKLLSVFRE 445
            P+ MTV WS  AQS+T + MKGMLTGP  IL W FVRN+ PR      I +AL DE+ DLEK     I + +  +REG  L       YL W    F ++ + V++       + YS F    P +  G   D      SRSD +LL+ F +
Sbjct:  530 PEPMTVRWSQYAQSLTDKVMKGMLTGPVTILQWSFVRNDIPRSTVCKQIGVALSDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLQWAGEAFRLSYMGVKDDTQIHTHMCYSEFNDILPAI-AGLDADVITIETSRSDMELLTAFAD 680
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_BLOFL (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Blochmannia floridanus GN=metE PE=3 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 1.126e-14
Identity = 43/106 (40.57%), Postives = 63/106 (59.43%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSL-GI*ACFYLDWGVPPFEITTVVVRN 316
            P  +T+ W + AQS+T +P+KG+LTGP  I+ W F R + PRH     +ALA++DE+ DLEK     I + +  LREG  L      +YL W +  F+IT   V++
Sbjct:  539 PIPITIPWINYAQSLTNKPVKGILTGPITIMTWSFAREDIPRHMIALQLALAIRDEVMDLEKSGIGVIQIDEPALREGLPLKKSEQSYYLKWAINTFKITVSSVKD 644
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_BAUCH (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Baumannia cicadellinicola subsp. Homalodisca coagulata GN=metE PE=3 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 1.358e-14
Identity = 47/106 (44.34%), Postives = 59/106 (55.66%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSLGI*AC-FYLDWGVPPFEITTVVVRN 316
            PK +TV W+  AQS+T +P+KGMLTGP  IL W F R +  R      IALAL+DE+ DLE      I + +  LREG  L   A   YL W V  F +   V +N
Sbjct:  534 PKPITVEWAWYAQSLTQKPVKGMLTGPVTILCWSFPREDLDRKTIARQIALALRDEVIDLENAGIGIIQIDEPALREGLPLKYSAWKEYLTWAVEAFRLNAAVAKN 639

HSP 2 Score: 20.7866 bits (42), Expect = 1.358e-14
Identity = 9/13 (69.23%), Postives = 10/13 (76.92%), Query Frame = 3 
Query:  369 IYTLDADVIPIET 407
            I  +DADVI IET
Sbjct:  659 IIAMDADVITIET 671
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_ECO5E (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Escherichia coli O157:H7 (strain EC4115 / EHEC) GN=metE PE=3 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 1.358e-14
Identity = 47/106 (44.34%), Postives = 58/106 (54.72%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSLGI*AC-FYLDWGVPPFEITTVVVRN 316
            P  +TV W+  AQSMT +P+KGMLTGP  IL W F R +  R      IALAL+DE+ DLE      I + +  LREG  L       YL WGV  F I   V ++
Sbjct:  529 PAPITVEWAKYAQSMTDKPVKGMLTGPVTILCWSFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEAFRINAAVAKD 634

HSP 2 Score: 21.1718 bits (43), Expect = 1.358e-14
Identity = 10/13 (76.92%), Postives = 10/13 (76.92%), Query Frame = 3 
Query:  369 IYTLDADVIPIET 407
            I  LDADVI IET
Sbjct:  654 IAALDADVITIET 666
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_ECO57 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Escherichia coli O157:H7 GN=metE PE=3 SV=3)

HSP 1 Score: 77.411 bits (189), Expect = 1.358e-14
Identity = 47/106 (44.34%), Postives = 58/106 (54.72%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSLGI*AC-FYLDWGVPPFEITTVVVRN 316
            P  +TV W+  AQSMT +P+KGMLTGP  IL W F R +  R      IALAL+DE+ DLE      I + +  LREG  L       YL WGV  F I   V ++
Sbjct:  529 PAPITVEWAKYAQSMTDKPVKGMLTGPVTILCWSFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEAFRINAAVAKD 634

HSP 2 Score: 21.1718 bits (43), Expect = 1.358e-14
Identity = 10/13 (76.92%), Postives = 10/13 (76.92%), Query Frame = 3 
Query:  369 IYTLDADVIPIET 407
            I  LDADVI IET
Sbjct:  654 IAALDADVITIET 666
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_SALPK (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Salmonella paratyphi A (strain AKU_12601) GN=metE PE=3 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 1.358e-14
Identity = 46/106 (43.40%), Postives = 59/106 (55.66%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSLGI*AC-FYLDWGVPPFEITTVVVRN 316
            P  +TV W+  AQS+T +P+KGMLTGP  IL W F R +  R      IALAL+DE+ DLE      I + +  LREG  L       YL+WGV  F I   V ++
Sbjct:  529 PAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPREDVTRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLEWGVEAFRINAAVAKD 634

HSP 2 Score: 21.557 bits (44), Expect = 1.358e-14
Identity = 10/13 (76.92%), Postives = 10/13 (76.92%), Query Frame = 3 
Query:  369 IYTLDADVIPIET 407
            I  LDADVI IET
Sbjct:  654 IVALDADVITIET 666
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_SALPA (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Salmonella paratyphi A GN=metE PE=3 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 1.358e-14
Identity = 46/106 (43.40%), Postives = 59/106 (55.66%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSLGI*AC-FYLDWGVPPFEITTVVVRN 316
            P  +TV W+  AQS+T +P+KGMLTGP  IL W F R +  R      IALAL+DE+ DLE      I + +  LREG  L       YL+WGV  F I   V ++
Sbjct:  529 PAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPREDVTRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLEWGVEAFRINAAVAKD 634

HSP 2 Score: 21.557 bits (44), Expect = 1.358e-14
Identity = 10/13 (76.92%), Postives = 10/13 (76.92%), Query Frame = 3 
Query:  369 IYTLDADVIPIET 407
            I  LDADVI IET
Sbjct:  654 IVALDADVITIET 666
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_SODGM (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Sodalis glossinidius (strain morsitans) GN=metE PE=3 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 1.471e-14
Identity = 46/106 (43.40%), Postives = 62/106 (58.49%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSLGI*AC-FYLDWGVPPFEITTVVVRN 316
            P+A+T+ W+  AQS+TA+P+KGMLTGP  IL W F R +  R      IALAL+DE+ DLE+     I + +  LREG  L   A   YL W V  F +   V ++
Sbjct:  532 PEAITIEWARYAQSLTAKPVKGMLTGPVTILCWSFPREDVSRKTIARQIALALRDEVADLEQAGIGVIQIDEPALREGLPLKHSAWQDYLTWAVEAFRLNAAVAQD 637
[Close]
BLAST of EY735758 vs. ExPASy Swiss-Prot
Match: METE_PASMU (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Pasteurella multocida GN=metE PE=3 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 1.471e-14
Identity = 59/150 (39.33%), Postives = 77/150 (51.33%), Query Frame = 2 
Query:    2 PKAMTVFWSSLAQSMTARPMKGMLTGPGHILHWVFVRNEPPRHENW*HIALALQDEIQDLEKGRFTFIPLIKVPLREGCSL-GI*ACFYLDWGVPPFEITTVVVRNYPD-SPPIWYSNFKKFPPHLYIGC*CD--PH*DSRSDEKLLSVF 439
            P+ MTV WS  AQS+T + MKGMLTGP  IL W FVRN+ PR      I +AL DE+ DLEK     I + +  +REG  L       YL W    F ++ + V++       + YS F    P +  G   D      SRSD +LL+ F
Sbjct:  531 PEPMTVRWSQYAQSLTNKVMKGMLTGPVTILQWSFVRNDIPRSTVCKQIGVALSDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLQWAGEAFRLSYMGVQDDTQIHTHMCYSEFNDILPAI-AGLDADVITIETSRSDMELLTAF 679
The following BLAST results are available for this feature:
BLAST of EY735758 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 242
Match NameE-valueIdentityDescription
METE_ACTP78.624e-1538.825-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_ACTP28.624e-1538.825-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BLOFL1.126e-1440.575-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BAUCH1.358e-1444.345-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_ECO5E1.358e-1444.345-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_ECO571.358e-1444.345-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_SALPK1.358e-1443.405-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_SALPA1.358e-1443.405-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_SODGM1.471e-1443.405-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_PASMU1.471e-1439.335-methyltetrahydropteroyltriglutamate--homocystein... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-705-004-F09-CT.F Sweet orange fruit, development stadium (6 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY735758 ID=EY735758; Name=EY735758; organism=Citrus sinensis; type=EST; length=464bp
TCCCAAGGCTATGACCGTCTTCTGGTCCTCCTTGGCTCAGAGCATGACTG
CTCGGCCAATGAAGGGAATGCTTACTGGCCCTGGTCACATTCTCCACTGG
GTCTTTGTCAGAAATGAGCCGCCCCGACATGAAAACTGGTAACACATTGC
TTTGGCCCTCCAGGACGAAATCCAGGATCTTGAGAAAGGGCGGTTTACGT
TTATCCCATTGATTAAGGTGCCCTTAAGGGAAGGATGCTCCTTAGGAATC
TGAGCCTGTTTCTACTTGGACTGGGGAGTCCCTCCTTTCGAAATCACCAC
TGTGGTGGTCCGAAACTACCCCGATTCCCCCCCCATTTGGTATTCCAATT
TCAAGAAATTTCCCCCCCATTTATACATTGGATGCTGATGTGATCCCCAT
TGAGACTCTCGTTCTGATGAGAAGCTCCTGTCTGTCTTCCGTGAGGGAGT
CANGTATGGTGCTG
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