EY735677

Overview
NameEY735677
Unique NameEY735677
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length900
Libraries
Library NameType
Sweet orange fruit, development stadium (6 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
EY735677_ssr21 microsatellite EY735677_ssr21:21..32. BLAST: Citrus ESTs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY735677 vs. ExPASy Swiss-Prot
Match: ARL4C_MOUSE (ADP-ribosylation factor-like protein 4C OS=Mus musculus GN=Arl4c PE=2 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 3.166e-32
Identity = 71/144 (49.31%), Postives = 96/144 (66.67%), Query Frame = 2 
Query:  113 SRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKN-----ISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVCG*QQDLPNAMNAGEITGQLGLHSL 529
            S + A + + I+M+GLD+AGKTT+LY+LK  E V T+PTIGFN E ++  N     IS   WDVGGQ+K+RPLW+ Y + T G+I+VVDS D DR+ EA+ ELH++    E +   LLV   +QDLP ++   EI  QL LH L
Sbjct:    6 SNISAFQSLHIVMLGLDSAGKTTVLYRLKFNEFVNTVPTIGFNTEKIKLSNGTAKGISCHFWDVGGQEKLRPLWKSYSRCTDGIIYVVDSVDVDRLEEAKTELHKVTKFAENQGTPLLVIANKQDLPKSLPVAEIEKQLALHEL 149

HSP 2 Score: 21.1718 bits (43), Expect = 3.166e-32
Identity = 7/17 (41.18%), Postives = 11/17 (64.71%), Query Frame = 3 
Query:  543 WYIHSTCATSGEGVFEG 593
            +++   CA  GEG+ EG
Sbjct:  155 YHVQPACAIIGEGLTEG 171
[Close]
BLAST of EY735677 vs. ExPASy Swiss-Prot
Match: ARL4C_HUMAN (ADP-ribosylation factor-like protein 4C OS=Homo sapiens GN=ARL4C PE=2 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 3.166e-32
Identity = 71/144 (49.31%), Postives = 96/144 (66.67%), Query Frame = 2 
Query:  113 SRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKN-----ISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVCG*QQDLPNAMNAGEITGQLGLHSL 529
            S + A + + I+M+GLD+AGKTT+LY+LK  E V T+PTIGFN E ++  N     IS   WDVGGQ+K+RPLW+ Y + T G+I+VVDS D DR+ EA+ ELH++    E +   LLV   +QDLP ++   EI  QL LH L
Sbjct:    6 SNISAFQSLHIVMLGLDSAGKTTVLYRLKFNEFVNTVPTIGFNTEKIKLSNGTAKGISCHFWDVGGQEKLRPLWKSYSRCTDGIIYVVDSVDVDRLEEAKTELHKVTKFAENQGTPLLVIANKQDLPKSLPVAEIEKQLALHEL 149

HSP 2 Score: 21.1718 bits (43), Expect = 3.166e-32
Identity = 7/17 (41.18%), Postives = 11/17 (64.71%), Query Frame = 3 
Query:  543 WYIHSTCATSGEGVFEG 593
            +++   CA  GEG+ EG
Sbjct:  155 YHVQPACAIIGEGLTEG 171
[Close]
BLAST of EY735677 vs. ExPASy Swiss-Prot
Match: ARL14_HUMAN (ADP-ribosylation factor-like protein 14 OS=Homo sapiens GN=ARL14 PE=1 SV=1)

HSP 1 Score: 133.65 bits (335), Expect = 3.166e-32
Identity = 60/126 (47.62%), Postives = 95/126 (75.40%), Query Frame = 2 
Query:  131 KEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEY-KNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVCG*QQDLPNAMNAGEIT 505
            K+ ++L++GLD+AGK+T+LYKLKL + +TTIPTIGFNVE +E  +N S TVWDVGGQ+K+R +W  Y +NT GL++VVDS D+ R+ E++ +   +L  + +++  +++   +QD+P A+ A +IT
Sbjct:   12 KQAQVLLLGLDSAGKSTLLYKLKLAKDITTIPTIGFNVEMIELERNFSLTVWDVGGQEKMRTVWGCYCENTDGLVYVVDSTDKQRLEESQRQFEHILKNEHIKNVPVVLLANKQDMPGALTAEDIT 137

HSP 2 Score: 26.5646 bits (57), Expect = 3.166e-32
Identity = 8/18 (44.44%), Postives = 13/18 (72.22%), Query Frame = 3 
Query:  540 HWYIHSTCATSGEGVFEG 593
            +WY+   CA +GEG+ +G
Sbjct:  150 NWYVQPCCALTGEGLAQG 167
[Close]
BLAST of EY735677 vs. ExPASy Swiss-Prot
Match: ARL2_BOVIN (ADP-ribosylation factor-like protein 2 OS=Bos taurus GN=ARL2 PE=2 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 2.465e-30
Identity = 64/135 (47.41%), Postives = 93/135 (68.89%), Query Frame = 2 
Query:  128 KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVCG*QQDLPNAMNAGEITGQLGLHSLR 532
            ++E+R+LM+GLD AGKTTIL K    +I T  PT+GFN++T+E++     +WDVGGQ  +R  WR+YF++T GLI+VVDS DR R+ + + EL  +L E+ L  A LL+   +QDLP A+++  I   L L S+R
Sbjct:   14 ERELRLLMLGLDNAGKTTILKKFNGEDIDTISPTLGFNIKTLEHRGFKLNIWDVGGQKSLRSYWRNYFESTDGLIWVVDSADRQRMQDCQRELQNLLVEERLAGATLLIFANKQDLPGALSSNAIREALELDSIR 148
[Close]
BLAST of EY735677 vs. ExPASy Swiss-Prot
Match: ARL2_HUMAN (ADP-ribosylation factor-like protein 2 OS=Homo sapiens GN=ARL2 PE=1 SV=4)

HSP 1 Score: 132.109 bits (331), Expect = 4.205e-30
Identity = 64/135 (47.41%), Postives = 93/135 (68.89%), Query Frame = 2 
Query:  128 KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVCG*QQDLPNAMNAGEITGQLGLHSLR 532
            ++E+R+LM+GLD AGKTTIL K    +I T  PT+GFN++T+E++     +WDVGGQ  +R  WR+YF++T GLI+VVDS DR R+ + + EL  +L E+ L  A LL+   +QDLP A+++  I   L L S+R
Sbjct:   14 ERELRLLMLGLDNAGKTTILKKFNGEDIDTISPTLGFNIKTLEHRGFKLNIWDVGGQKSLRSYWRNYFESTDGLIWVVDSADRQRMQDCQRELQSLLVEERLAGATLLIFANKQDLPGALSSNAIREVLELDSIR 148
[Close]
BLAST of EY735677 vs. ExPASy Swiss-Prot
Match: ARL2_MOUSE (ADP-ribosylation factor-like protein 2 OS=Mus musculus GN=Arl2 PE=1 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 5.492e-30
Identity = 63/135 (46.67%), Postives = 92/135 (68.15%), Query Frame = 2 
Query:  128 KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVCG*QQDLPNAMNAGEITGQLGLHSLR 532
            ++E+R+LM+GLD AGKTTIL K    ++ T  PT+GFN++T+E++     +WDVGGQ  +R  WR+YF++T GLI+VVDS DR R+ + + EL  +L E+ L  A LL+   +QDLP A++   I   L L S+R
Sbjct:   14 ERELRLLMLGLDNAGKTTILKKFNGEDVDTISPTLGFNIKTLEHRGFKLNIWDVGGQKSLRSYWRNYFESTDGLIWVVDSADRQRMQDCQRELQSLLVEERLAGATLLIFANKQDLPGALSCNAIQEALELDSIR 148
[Close]
BLAST of EY735677 vs. ExPASy Swiss-Prot
Match: ARL2_CAEEL (ADP-ribosylation factor-like protein 2 OS=Caenorhabditis elegans GN=evl-20 PE=2 SV=2)

HSP 1 Score: 129.798 bits (325), Expect = 6.237e-30
Identity = 66/149 (44.30%), Postives = 102/149 (68.46%), Query Frame = 2 
Query:   92 LSFTKLFSRLFAK-KEMRILMVGLDAAGKTTILYKLKLGEIVTTI-PTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVCG*QQDLPNAMNAGEITGQLGLHSLR 532
            + F K+  +  A+ +EMRIL++GLD AGKTT++ K  L E   TI PT+GF+++TV +K+    +WDVGGQ  +R  W++YF++T  LI+VVDS+DR+R+++  +EL ++L E+ L  A LLV   + DLP A++   I   L LHS++
Sbjct:    1 MGFLKILRKQRAREREMRILILGLDNAGKTTLMKKF-LDEPTDTIEPTLGFDIKTVHFKDFQLNLWDVGGQKSLRSYWKNYFESTDALIWVVDSSDRERLLQCSEELKKLLGEERLAGASLLVLANKSDLPGAIDVNSIAQVLDLHSIK 148

HSP 2 Score: 22.7126 bits (47), Expect = 6.237e-30
Identity = 8/13 (61.54%), Postives = 9/13 (69.23%), Query Frame = 3 
Query:  540 HWYIHSTCATSGE 578
            HW I S CA SG+
Sbjct:  151 HWKIFSCCALSGD 163
[Close]
BLAST of EY735677 vs. ExPASy Swiss-Prot
Match: ARL2_RAT (ADP-ribosylation factor-like protein 2 OS=Rattus norvegicus GN=Arl2 PE=2 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 1.598e-29
Identity = 62/135 (45.93%), Postives = 92/135 (68.15%), Query Frame = 2 
Query:  128 KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVCG*QQDLPNAMNAGEITGQLGLHSLR 532
            ++++R+LM+GLD AGKTTIL K    ++ T  PT+GFN++T+E++     +WDVGGQ  +R  WR+YF++T GLI+VVDS DR R+ + + EL  +L E+ L  A LL+   +QDLP A++   I   L L S+R
Sbjct:   14 ERDVRLLMLGLDNAGKTTILKKFNGEDVDTISPTLGFNIKTLEHRGFKLNIWDVGGQKSLRSYWRNYFESTDGLIWVVDSADRQRMQDCQRELQSLLVEERLAGATLLIFANKQDLPGALSCNAIQEALELDSIR 148
[Close]
BLAST of EY735677 vs. ExPASy Swiss-Prot
Match: ARL4A_HUMAN (ADP-ribosylation factor-like protein 4A OS=Homo sapiens GN=ARL4A PE=2 SV=2)

HSP 1 Score: 125.561 bits (314), Expect = 3.031e-29
Identity = 62/148 (41.89%), Postives = 95/148 (64.19%), Query Frame = 2 
Query:  101 TKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEY-----KNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVCG*QQDLPNAMNAGEITGQLGLHSL 529
            T + S L + +   I+++GLD AGKTT+LY+L+  E V T+PT GFN E ++      K ++F  WDVGGQ+K+RPLW+ Y + T G++FVVDS D +R+ EA+ ELH++    E +   +L+   +QDL N+++  EI   L +  L
Sbjct:    9 TSILSNLPSFQSFHIVILGLDCAGKTTVLYRLQFNEFVNTVPTKGFNTEKIKVTLGNSKTVTFHFWDVGGQEKLRPLWKSYTRCTDGIVFVVDSVDVERMEEAKTELHKITRISENQGVPVLIVANKQDLRNSLSLSEIEKLLAMGEL 156

HSP 2 Score: 24.6386 bits (52), Expect = 3.031e-29
Identity = 8/17 (47.06%), Postives = 12/17 (70.59%), Query Frame = 3 
Query:  543 WYIHSTCATSGEGVFEG 593
            W++  TCA  G+G+ EG
Sbjct:  162 WHLQPTCAIIGDGLKEG 178
[Close]
BLAST of EY735677 vs. ExPASy Swiss-Prot
Match: ARL4A_RAT (ADP-ribosylation factor-like protein 4A OS=Rattus norvegicus GN=Arl4a PE=2 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 3.947e-29
Identity = 62/148 (41.89%), Postives = 95/148 (64.19%), Query Frame = 2 
Query:  101 TKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEY-----KNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVCG*QQDLPNAMNAGEITGQLGLHSL 529
            T + S L + +   I+++GLD AGKTT+LY+L+  E V T+PT GFN E ++      K ++F  WDVGGQ+K+RPLW+ Y + T G++FVVDS D +R+ EA+ ELH++    E +   +L+   +QDL N+++  EI   L +  L
Sbjct:    9 TSILSSLPSFQSFHIVILGLDCAGKTTVLYRLQFNEFVNTVPTKGFNTEKIKVTLGNSKTVTFHFWDVGGQEKLRPLWKSYTRCTDGIVFVVDSVDVERMEEAKTELHKITRISENQGVPVLIVANKQDLRNSLSLSEIEKLLAMGEL 156

HSP 2 Score: 24.6386 bits (52), Expect = 3.947e-29
Identity = 8/17 (47.06%), Postives = 12/17 (70.59%), Query Frame = 3 
Query:  543 WYIHSTCATSGEGVFEG 593
            W++  TCA  G+G+ EG
Sbjct:  162 WHLQPTCAIIGDGLKEG 178
The following BLAST results are available for this feature:
BLAST of EY735677 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 217
Match NameE-valueIdentityDescription
ARL4C_MOUSE3.166e-3249.31ADP-ribosylation factor-like protein 4C OS=Mus mus... [more]
ARL4C_HUMAN3.166e-3249.31ADP-ribosylation factor-like protein 4C OS=Homo sa... [more]
ARL14_HUMAN3.166e-3247.62ADP-ribosylation factor-like protein 14 OS=Homo sa... [more]
ARL2_BOVIN2.465e-3047.41ADP-ribosylation factor-like protein 2 OS=Bos taur... [more]
ARL2_HUMAN4.205e-3047.41ADP-ribosylation factor-like protein 2 OS=Homo sap... [more]
ARL2_MOUSE5.492e-3046.67ADP-ribosylation factor-like protein 2 OS=Mus musc... [more]
ARL2_CAEEL6.237e-3044.30ADP-ribosylation factor-like protein 2 OS=Caenorha... [more]
ARL2_RAT1.598e-2945.93ADP-ribosylation factor-like protein 2 OS=Rattus n... [more]
ARL4A_HUMAN3.031e-2941.89ADP-ribosylation factor-like protein 4A OS=Homo sa... [more]
ARL4A_RAT3.947e-2941.89ADP-ribosylation factor-like protein 4A OS=Rattus ... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-705-003-F01-CT.F Sweet orange fruit, development stadium (6 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY735677 ID=EY735677; Name=EY735677; organism=Citrus sinensis; type=EST; length=900bp
CACGCGTCCGGTATTTTTATAGAGAGAGAGAGGGGCAGTAGATCTGCACC
AAGGCAAAAACCGCTCTCTTCCTCAGTTGTGAAAAATGGGGCTGTCTTTC
ACCAAGCTTTTCAGCAGGCTTTTTGCTAAGAAAGAGATGAGAATTCTCAT
GGTAGGTCTCGATGCAGCTGGTAAGACTACTATCCTGTACAAGCTTAAGC
TCGGAGAGATCGTCACCACCATTCCTACCATTGGATTTAATGTAGAGACC
GTTGAGTATAAGAACATCAGCTTTACTGTCTGGGATGTCGGGGGTCAAGA
CAAGATCCGACCTTTGTGGAGACATTATTTCCAAAACACCCAAGGACTCA
TCTTTGTGGTTGACAGTAATGATCGCGACCGTGTGGTAGAAGCTAGGGAT
GAGTTGCACAGAATGTTGAATGAGGATGAATTGAGAGATGCTGTGCTGCT
TGTATGTGGCTAACAGCAAGATCTTCCAAATGCAATGAATGCTGGTGAGA
TAACTGGACAGCTTGGCCTTCACTCTCTTCGGCGGGGCCCATTGGTATAT
CCATAGCACCTGTGCCACTTCTGGTGAAGGGGTGTTTGAGGGGACTTGAC
TGGCGTCGGGGAGGGGTGGGGGGGTAGGGGTGTGACCACTCCACTTTTTA
TTTGTGGGTTGGCGGTAGCGGGCGAAAGACCAGAGTCATTATGTGGTTTT
TGGTGGTTCTTGTGTTTGCACCCGCCGGATTATGTGGGGTGGTCGCGGTT
TCTGTGGAGCTGGCTGTGTCCGCTGTCGTCGTGTCTGGGATTGTGTTGCG
GTGCTGCTGGGTTAGGACGCCCGGCACGCCTGTCACCAGCCCGGGAAGAC
TCGAAGCGGTGTGGAGGCCTGAGAGCATCCCCACGGGCAGCCCGGGGGCG
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