EY735673 | Citrus Genome Database

Overview


Name	EY735673
Unique Name	EY735673
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	861

Libraries


Library Name	Type
Sweet orange fruit, development stadium (6 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY735673 vs. ExPASy Swiss-Prot
Match: INO1_MESCR (Inositol-3-phosphate synthase OS=Mesembryanthemum crystallinum PE=2 SV=1)

HSP 1 Score: 252.677 bits (644), Expect = 6.577e-75
Identity = 120/141 (85.11%), Postives = 132/141 (93.62%), Query Frame = 3

Query:  264 MFIENFKVESPNVKYTDHEIHSVYDYETTELVHENRN--GTYQWIVKPKTVKYEFKTDVHVPKLGVMLVGWGGNNGSTLTGGVISNREGISWATKDTVQQANYFGSLTQASAIRVGSYNGEEIYAPFKSILPMVNPDDIVF 680
            MFIE+FKVESPNVKYT++EI SVY+Y+TTELVHENR   G YQWIVKPKTV+Y FKTD  VPKLGVMLVGWGGNNGSTLTGGVI+NREGISWATKD +QQANYFGSLTQAS+IRVGS+NGEEIYAPFKS+LPMVNPDD+VF
Sbjct:    1 MFIESFKVESPNVKYTENEIESVYNYDTTELVHENRKDAGGYQWIVKPKTVQYHFKTDTRVPKLGVMLVGWGGNNGSTLTGGVIANREGISWATKDKIQQANYFGSLTQASSIRVGSFNGEEIYAPFKSLLPMVNPDDVVF 141

HSP 2 Score: 40.4318 bits (93), Expect = 6.577e-75
Identity = 16/31 (51.61%), Postives = 23/31 (74.19%), Query Frame = 2

Query:  722 LARAKMLGYDLQKQFKAYLESMVPLPGNFEP 814
            + RA++   DLQKQ + Y+E MVPLPG ++P
Sbjct:  155 MTRARVFDIDLQKQLRPYMEHMVPLPGIYDP 185

HSP 3 Score: 31.187 bits (69), Expect = 6.577e-75
Identity = 12/15 (80.00%), Postives = 14/15 (93.33%), Query Frame = 1

Query:  679 FGGWDISGLNLADAI 723
            FGGWDIS +NLADA+
Sbjct:  141 FGGWDISDMNLADAM 155

[Close]

BLAST of EY735673 vs. ExPASy Swiss-Prot
Match: INO1_SPIPO (Inositol-3-phosphate synthase OS=Spirodela polyrrhiza GN=TUR1 PE=2 SV=1)

HSP 1 Score: 244.973 bits (624), Expect = 1.116e-74
Identity = 114/139 (82.01%), Postives = 128/139 (92.09%), Query Frame = 3

Query:  264 MFIENFKVESPNVKYTDHEIHSVYDYETTELVHENRNGTYQWIVKPKTVKYEFKTDVHVPKLGVMLVGWGGNNGSTLTGGVISNREGISWATKDTVQQANYFGSLTQASAIRVGSYNGEEIYAPFKSILPMVNPDDIVF 680
            MFIE F+VESPNVKY D EI SVY YETTELVHE RNG+YQW+VKPK+V+Y+FKTD  VP+LGVMLVGWGGNNGSTLT GVI+NREGISW TK+ VQQANYFGSLTQ+S+IRVGS+NGEEIYAPFKS+LPMVNPD+IVF
Sbjct:    1 MFIEKFRVESPNVKYGDGEIESVYSYETTELVHEVRNGSYQWVVKPKSVQYQFKTDTRVPRLGVMLVGWGGNNGSTLTAGVIANREGISWVTKEKVQQANYFGSLTQSSSIRVGSFNGEEIYAPFKSLLPMVNPDEIVF 139

HSP 2 Score: 44.2838 bits (103), Expect = 1.116e-74
Identity = 19/31 (61.29%), Postives = 24/31 (77.42%), Query Frame = 2

Query:  722 LARAKMLGYDLQKQFKAYLESMVPLPGNFEP 814
            + RAK+L  DLQKQ + Y+ESMVPLPG + P
Sbjct:  153 MGRAKVLDIDLQKQLRPYMESMVPLPGIYNP 183

HSP 3 Score: 34.2686 bits (77), Expect = 1.116e-74
Identity = 13/19 (68.42%), Postives = 17/19 (89.47%), Query Frame = 1

Query:  673 LCFGGWDISGLNLADAIGQ 729
            + FGGWDIS +NLADA+G+
Sbjct:  137 IVFGGWDISDMNLADAMGR 155

[Close]

BLAST of EY735673 vs. ExPASy Swiss-Prot
Match: INO1_ORYSJ (Inositol-3-phosphate synthase OS=Oryza sativa subsp. japonica GN=INO1 PE=2 SV=2)

HSP 1 Score: 228.024 bits (580), Expect = 2.117e-69
Identity = 107/139 (76.98%), Postives = 123/139 (88.49%), Query Frame = 3

Query:  264 MFIENFKVESPNVKYTDHEIHSVYDYETTELVHENRNGTYQWIVKPKTVKYEFKTDVHVPKLGVMLVGWGGNNGSTLTGGVISNREGISWATKDTVQQANYFGSLTQASAIRVGSYNGEEIYAPFKSILPMVNPDDIVF 680
            MFIE+F+VESP+V+Y   EI S Y Y+TTELVHE+ +G  +WIV+PK+V+Y F+T   VPKLGVMLVGWGGNNGSTLT GVI+NREGISWATKD VQQANY+GSLTQAS IRVGSYNGEEIYAPFKS+LPMVNPDD+VF
Sbjct:    1 MFIESFRVESPHVRYGAAEIESDYQYDTTELVHESHDGASRWIVRPKSVRYNFRTTTTVPKLGVMLVGWGGNNGSTLTAGVIANREGISWATKDKVQQANYYGSLTQASTIRVGSYNGEEIYAPFKSLLPMVNPDDLVF 139

HSP 2 Score: 45.0542 bits (105), Expect = 2.117e-69
Identity = 19/31 (61.29%), Postives = 25/31 (80.65%), Query Frame = 2

Query:  722 LARAKMLGYDLQKQFKAYLESMVPLPGNFEP 814
            + RAK+L  DLQKQ + Y+ESMVPLPG ++P
Sbjct:  153 MTRAKVLDIDLQKQLRPYMESMVPLPGIYDP 183

HSP 3 Score: 32.7278 bits (73), Expect = 2.117e-69
Identity = 13/17 (76.47%), Postives = 15/17 (88.24%), Query Frame = 1

Query:  673 LCFGGWDISGLNLADAI 723
            L FGGWDIS +NLADA+
Sbjct:  137 LVFGGWDISNMNLADAM 153

[Close]

BLAST of EY735673 vs. ExPASy Swiss-Prot
Match: INO1_MAIZE (Inositol-3-phosphate synthase OS=Zea mays PE=2 SV=2)

HSP 1 Score: 229.565 bits (584), Expect = 2.757e-69
Identity = 108/139 (77.70%), Postives = 123/139 (88.49%), Query Frame = 3

Query:  264 MFIENFKVESPNVKYTDHEIHSVYDYETTELVHENRNGTYQWIVKPKTVKYEFKTDVHVPKLGVMLVGWGGNNGSTLTGGVISNREGISWATKDTVQQANYFGSLTQASAIRVGSYNGEEIYAPFKSILPMVNPDDIVF 680
            MFIE+F+VESP+V+Y   EI S Y Y+TTELVHE ++G  +W+V+PK+VKY F+T   VPKLGVMLVGWGGNNGSTLT GVI+NREGISWATKD VQQANY+GSLTQAS IRVGSYNGEEIYAPFKS+LPMVNPDDIVF
Sbjct:    1 MFIESFRVESPHVRYGPMEIESEYRYDTTELVHEGKDGASRWVVRPKSVKYNFRTRTAVPKLGVMLVGWGGNNGSTLTAGVIANREGISWATKDKVQQANYYGSLTQASTIRVGSYNGEEIYAPFKSLLPMVNPDDIVF 139

HSP 2 Score: 45.0542 bits (105), Expect = 2.757e-69
Identity = 19/31 (61.29%), Postives = 25/31 (80.65%), Query Frame = 2

Query:  722 LARAKMLGYDLQKQFKAYLESMVPLPGNFEP 814
            + RAK+L  DLQKQ + Y+ESMVPLPG ++P
Sbjct:  153 MTRAKVLDIDLQKQLRPYMESMVPLPGIYDP 183

HSP 3 Score: 30.8018 bits (68), Expect = 2.757e-69
Identity = 11/17 (64.71%), Postives = 15/17 (88.24%), Query Frame = 1

Query:  673 LCFGGWDISGLNLADAI 723
            + FGGWDIS +NLAD++
Sbjct:  137 IVFGGWDISNMNLADSM 153

[Close]

BLAST of EY735673 vs. ExPASy Swiss-Prot
Match: INO1_HORVU (Inositol-3-phosphate synthase OS=Hordeum vulgare PE=2 SV=1)

HSP 1 Score: 226.868 bits (577), Expect = 1.751e-68
Identity = 107/139 (76.98%), Postives = 121/139 (87.05%), Query Frame = 3

Query:  264 MFIENFKVESPNVKYTDHEIHSVYDYETTELVHENRNGTYQWIVKPKTVKYEFKTDVHVPKLGVMLVGWGGNNGSTLTGGVISNREGISWATKDTVQQANYFGSLTQASAIRVGSYNGEEIYAPFKSILPMVNPDDIVF 680
            MFIE+F+VESP V+Y   EI S Y Y+TTELVHE+ +G  +W+V+PK+V Y FKT+  VPKLGVMLVGWGGNNGSTL  GVI+NREGISWATKD VQQANYFGSLTQAS IRVGSYNGEEIYAPFKS+LPMVNPDD+VF
Sbjct:    1 MFIESFRVESPKVRYGAGEIESEYRYDTTELVHESHDGASKWVVRPKSVNYHFKTNTTVPKLGVMLVGWGGNNGSTLMAGVIANREGISWATKDKVQQANYFGSLTQASTIRVGSYNGEEIYAPFKSLLPMVNPDDLVF 139

HSP 2 Score: 43.1282 bits (100), Expect = 1.751e-68
Identity = 18/31 (58.06%), Postives = 24/31 (77.42%), Query Frame = 2

Query:  722 LARAKMLGYDLQKQFKAYLESMVPLPGNFEP 814
            + RAK+L  DLQKQ + Y+ESM PLPG ++P
Sbjct:  153 MTRAKVLDIDLQKQLRPYMESMCPLPGIYDP 183

HSP 3 Score: 32.7278 bits (73), Expect = 1.751e-68
Identity = 13/17 (76.47%), Postives = 15/17 (88.24%), Query Frame = 1

Query:  673 LCFGGWDISGLNLADAI 723
            L FGGWDIS +NLADA+
Sbjct:  137 LVFGGWDISSMNLADAM 153

[Close]

BLAST of EY735673 vs. ExPASy Swiss-Prot
Match: INO1_DICDI (Inositol-3-phosphate synthase OS=Dictyostelium discoideum GN=ino1 PE=3 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 5.598e-39
Identity = 75/137 (54.74%), Postives = 100/137 (72.99%), Query Frame = 3

Query:  273 ENFKVESPNVKYTDHEIHSVYDYETTELVHENRNGTYQWIVKPKTVKYEFKTDVHVPKLGVMLVGWGGNNGSTLTGGVISNREGISWATKDTVQQANYFGSLTQASAIRVG-SYNGEEIYAPFKSILPMVNPDDIVF 680
            E+FKV SPNVKYTD  I S Y Y+TT++  +N NG  + IV+P   KY FKT+  VP++GVM+VG  GNNG+T+ GGVI+NREG+ W TK  +Q  NYFGS+  +S IR+G   NG + Y P K+++PMV+P+DIVF
Sbjct:    7 ESFKVNSPNVKYTDEHIISDYTYQTTKV--QNVNG--ELIVEPVDQKYIFKTERKVPRMGVMIVGLCGNNGTTVVGGVIANREGLCWNTKQGLQTPNYFGSVVMSSTIRMGMDENGCDAYIPLKNLIPMVHPNDIVF 139

HSP 2 Score: 29.6462 bits (65), Expect = 5.598e-39
Identity = 11/17 (64.71%), Postives = 14/17 (82.35%), Query Frame = 1

Query:  673 LCFGGWDISGLNLADAI 723
            + FGGWDI+  NLADA+
Sbjct:  137 IVFGGWDINNANLADAM 153

HSP 3 Score: 28.1054 bits (61), Expect = 5.598e-39
Identity = 11/31 (35.48%), Postives = 20/31 (64.52%), Query Frame = 2

Query:  722 LARAKMLGYDLQKQFKAYLESMVPLPGNFEP 814
            + RA++  YDLQ Q   +++++ PLP  + P
Sbjct:  153 MQRAQVFDYDLQVQLIPHMKNITPLPSIYFP 183

[Close]

BLAST of EY735673 vs. ExPASy Swiss-Prot
Match: INO1B_XENLA (Inositol-3-phosphate synthase 1-B OS=Xenopus laevis GN=isyna1-B PE=2 SV=2)

HSP 1 Score: 141.739 bits (356), Expect = 1.227e-38
Identity = 70/136 (51.47%), Postives = 97/136 (71.32%), Query Frame = 3

Query:  273 ENFKVESPNVKYTDHEIHSVYDYETTELVHENRNGTYQWIVKPKTVKYEFKTDVHVPKLGVMLVGWGGNNGSTLTGGVISNREGISWATKDTVQQANYFGSLTQASAIRVGSYNGEEIYAPFKSILPMVNPDDIVF 680
            E FKVESP+V+Y    I + Y Y+TT+ V+E +  T    VKP + K+ F T+  VPKLGVMLVGWGGNNG+T+T  V++NR G+SW TK   + ANY+GSL Q+S + +G  +  E++ PF+ +LPMVNP+D+VF
Sbjct:    3 EMFKVESPHVRYLKDVIEADYSYDTTQ-VYEEKGVTK---VKPCSTKFTFHTERKVPKLGVMLVGWGGNNGTTVTAAVLANRLGLSWMTKTGKKVANYYGSLFQSSTVCLGRGSSGEVFVPFRDLLPMVNPNDLVF 134

HSP 2 Score: 30.8018 bits (68), Expect = 1.227e-38
Identity = 13/17 (76.47%), Postives = 14/17 (82.35%), Query Frame = 1

Query:  673 LCFGGWDISGLNLADAI 723
            L F GWDIS LNLADA+
Sbjct:  132 LVFDGWDISSLNLADAM 148

HSP 3 Score: 29.6462 bits (65), Expect = 1.227e-38
Identity = 12/29 (41.38%), Postives = 19/29 (65.52%), Query Frame = 2

Query:  728 RAKMLGYDLQKQFKAYLESMVPLPGNFEP 814
            RA++L + LQ+Q + Y+E M P P  + P
Sbjct:  150 RAEVLDWQLQEQLRPYMEKMKPRPSIYIP 178

[Close]

BLAST of EY735673 vs. ExPASy Swiss-Prot
Match: INO1A_XENLA (Inositol-3-phosphate synthase 1-A OS=Xenopus laevis GN=isyna1-A PE=2 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 1.299e-37
Identity = 70/136 (51.47%), Postives = 97/136 (71.32%), Query Frame = 3

Query:  273 ENFKVESPNVKYTDHEIHSVYDYETTELVHENRNGTYQWIVKPKTVKYEFKTDVHVPKLGVMLVGWGGNNGSTLTGGVISNREGISWATKDTVQQANYFGSLTQASAIRVGSYNGEEIYAPFKSILPMVNPDDIVF 680
            E FKVESP+V+Y    I + Y+Y+TT+ V+E +  T    VKP + K+ F T+  VPKLGVMLVGWGGNNG+T+T  V++NR G+SW TK   + ANY+GSL Q+S   +GS    +++ PF+ +LPMV+P+DIVF
Sbjct:    3 EKFKVESPHVRYLKDVIEADYNYDTTQ-VYEEKGVTK---VKPCSTKFTFHTERKVPKLGVMLVGWGGNNGTTVTAAVLANRLGLSWMTKTGKKVANYYGSLFQSSTACLGSGPAGDVFVPFRDLLPMVHPNDIVF 134

HSP 2 Score: 30.0314 bits (66), Expect = 1.299e-37
Identity = 12/17 (70.59%), Postives = 14/17 (82.35%), Query Frame = 1

Query:  673 LCFGGWDISGLNLADAI 723
            + F GWDIS LNLADA+
Sbjct:  132 IVFDGWDISSLNLADAM 148

HSP 3 Score: 28.8758 bits (63), Expect = 1.299e-37
Identity = 11/29 (37.93%), Postives = 19/29 (65.52%), Query Frame = 2

Query:  728 RAKMLGYDLQKQFKAYLESMVPLPGNFEP 814
            RA++L + +Q+Q + Y+E M P P  + P
Sbjct:  150 RAEVLDWQIQEQLRPYMEKMKPRPSIYIP 178

[Close]

BLAST of EY735673 vs. ExPASy Swiss-Prot
Match: INO1_HUMAN (Inositol-3-phosphate synthase 1 OS=Homo sapiens GN=ISYNA1 PE=1 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 9.585e-34
Identity = 65/135 (48.15%), Postives = 91/135 (67.41%), Query Frame = 3

Query:  279 FKVESPNVKYTDHEIHSVYDYETTELVHENRNGTYQWIVKPKTVKYEFKTDVHVPKLGVMLVGWGGNNGSTLTGGVISNREGISWATKDTVQQANYFGSLTQASAIRVG-SYNGEEIYAPFKSILPMVNPDDIVF 680
            F VESP+V Y    I + Y+Y TT +  E   G  +  V P + ++ F+T   VP+LGVMLVGWGGNNGSTLT  V++NR  +SW T+   ++ANY+GSLTQA  + +G    G+E++ PF ++LPMV P+D+VF
Sbjct:    7 FFVESPDVVYGPEAIEAQYEYRTTRVSREG--GVLK--VHPTSTRFTFRTARQVPRLGVMLVGWGGNNGSTLTAAVLANRLRLSWPTRSGRKEANYYGSLTQAGTVSLGLDAEGQEVFVPFSAVLPMVAPNDLVF 137

HSP 2 Score: 29.261 bits (64), Expect = 9.585e-34
Identity = 12/17 (70.59%), Postives = 14/17 (82.35%), Query Frame = 1

Query:  673 LCFGGWDISGLNLADAI 723
            L F GWDIS LNLA+A+
Sbjct:  135 LVFDGWDISSLNLAEAM 151

HSP 3 Score: 26.1794 bits (56), Expect = 9.585e-34
Identity = 11/31 (35.48%), Postives = 20/31 (64.52%), Query Frame = 2

Query:  722 LARAKMLGYDLQKQFKAYLESMVPLPGNFEP 814
            + RAK+L + LQ+Q   ++E++ P P  + P
Sbjct:  151 MRRAKVLDWGLQEQLWPHMEALRPRPSVYIP 181

[Close]

BLAST of EY735673 vs. ExPASy Swiss-Prot
Match: INO1_MACFA (Inositol-3-phosphate synthase 1 OS=Macaca fascicularis GN=ISYNA1 PE=2 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 1.245e-33
Identity = 65/135 (48.15%), Postives = 91/135 (67.41%), Query Frame = 3

Query:  279 FKVESPNVKYTDHEIHSVYDYETTELVHENRNGTYQWIVKPKTVKYEFKTDVHVPKLGVMLVGWGGNNGSTLTGGVISNREGISWATKDTVQQANYFGSLTQASAIRVG-SYNGEEIYAPFKSILPMVNPDDIVF 680
            F VESP+V Y    I + Y+Y TT +  E   G  +  V P + ++ F+T   VP+LGVMLVGWGGNNGSTLT  V++NR  +SW T+   ++ANY+GSLTQA  + +G    G+E++ PF ++LPMV P+D+VF
Sbjct:    7 FFVESPDVVYGPEAIEAQYEYRTTRVSREG--GVLK--VHPTSTRFTFRTARQVPRLGVMLVGWGGNNGSTLTAAVLANRLRLSWPTRSGRKEANYYGSLTQAGTVSLGLDAEGQEVFVPFSALLPMVAPNDLVF 137

HSP 2 Score: 29.261 bits (64), Expect = 1.245e-33
Identity = 12/17 (70.59%), Postives = 14/17 (82.35%), Query Frame = 1

Query:  673 LCFGGWDISGLNLADAI 723
            L F GWDIS LNLA+A+
Sbjct:  135 LVFDGWDISSLNLAEAM 151

HSP 3 Score: 26.1794 bits (56), Expect = 1.245e-33
Identity = 11/31 (35.48%), Postives = 20/31 (64.52%), Query Frame = 2

Query:  722 LARAKMLGYDLQKQFKAYLESMVPLPGNFEP 814
            + RAK+L + LQ+Q   ++E++ P P  + P
Sbjct:  151 MRRAKVLDWGLQEQLWPHMEALRPRPSVYIP 181

The following BLAST results are available for this feature:

BLAST of EY735673 vs. ExPASy Swiss-Prot

BLAST of EY735673 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 27


Match Name	E-value	Identity	Description
INO1_MESCR	6.577e-75	85.11	Inositol-3-phosphate synthase OS=Mesembryanthemum ...	[more]
INO1_SPIPO	1.116e-74	82.01	Inositol-3-phosphate synthase OS=Spirodela polyrrh...	[more]
INO1_ORYSJ	2.117e-69	76.98	Inositol-3-phosphate synthase OS=Oryza sativa subs...	[more]
INO1_MAIZE	2.757e-69	77.70	Inositol-3-phosphate synthase OS=Zea mays PE=2 SV=...	[more]
INO1_HORVU	1.751e-68	76.98	Inositol-3-phosphate synthase OS=Hordeum vulgare P...	[more]
INO1_DICDI	5.598e-39	54.74	Inositol-3-phosphate synthase OS=Dictyostelium dis...	[more]
INO1B_XENLA	1.227e-38	51.47	Inositol-3-phosphate synthase 1-B OS=Xenopus laevi...	[more]
INO1A_XENLA	1.299e-37	51.47	Inositol-3-phosphate synthase 1-A OS=Xenopus laevi...	[more]
INO1_HUMAN	9.585e-34	48.15	Inositol-3-phosphate synthase 1 OS=Homo sapiens GN...	[more]
INO1_MACFA	1.245e-33	48.15	Inositol-3-phosphate synthase 1 OS=Macaca fascicul...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C3-705-003-E08-CT.F Sweet orange fruit, development stadium (6 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY735673 ID=EY735673; Name=EY735673; organism=Citrus sinensis; type=EST; length=861bp
CCACGCGTCCGCTAGTTTCGAAAGTTAAAAGAGTGTTGAGTCGAGTGGGA
AGTGAGAATTGAGAAGGAGAGTTGAGTTCGGAGACAGCAAGACGAAACAG
CTCTTCTTCCAATAAATCTCAAAAGCATTTGTTTGGTTTTATTTAAAGCA
ATTTTCTCAGCAAACTCTTCTTCTAATAAATTTTAAAGCATTTGTTTGGT
TTTTATTTAAAGCAATTTTCTCAGCGAAGTTTTCAATTTTTTCTCTAAAA
TTTTCCTGGGAAAATGTTTATCGAAAATTTCAAGGTTGAGAGCCCAAATG
TGAAGTACACAGATCATGAGATTCACTCTGTGTACGATTATGAAACCACT
GAGCTTGTTCATGAGAACAGAAATGGCACTTATCAGTGGATTGTCAAGCC
CAAAACTGTCAAATATGAGTTCAAAACCGATGTTCATGTCCCTAAACTTG
GGGTTATGCTTGTGGGTTGGGGTGGAAACAACGGCTCAACTCTCACGGGT
GGTGTTATCTCGAATCGAGAGGGAATCTCCTGGGCCACAAAGGACACTGT
GCAACAAGCCAATTATTTTGGTTCACTTACCCAAGCATCAGCAATTAGAG
TTGGGTCTTACAATGGAGAAGAGATTTATGCTCCATTCAAGAGCATTCTC
CCTATGGTGAACCCAGATGACATTGTGTTTTGGAGGATGGGACATTAGTG
GACTGAACTTGGCTGATGCCATTGGCCAGGGCTAAAATGTTGGGATATGA
TCTTCAAAAACAATTTAAGGCGTACTTGGAATCAATGGTCCCACTTCCTG
GAAACTTTGAACCTTGAGTGCCTTGCTGCCAATCAGCGGGCCCGTGCCAA
CAACCTGATCG